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All Journal Mutiara Medika: Jurnal Kedokteran dan Kesehatan International Journal of Cell and Biomedical Science
Agus Widyatmoko, Agus
Bagian Ilmu Penyakit Dalam, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Muhammadiyah Yogyakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology

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Kadar Glukosa Darah pada Penderita Infark Miokard Akut dengan Diabetes Melitus sebagai Faktor Prediktor Kematian Sari, Rina Puspita; Widyatmoko, Agus
Jurnal Mutiara Medika Vol 12, No 2 (2012)
Publisher : Fakultas Kedokteran dan Ilmu Kesehatan Universitas Muhammadiyah Yogyakarta

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Abstract

Infark miokard akut (IMA) merupakan penyebab kematian pertama di Indonesia. Insiden IMA tergantung pada risiko terjadinya aterosklerosis, salah satunya diabetes melitus (DM). Pada pasien DM, kadar glukosa akan meningkat dan beberapa penelitian melaporkan hubungan antara abnormalitas glukosa dan mortalitas tetapi hingga saat ini belum jelas. Penelitian ini bertujuan untuk mengetahui peran kadar glukosa darah terhadap mortalitas pada penderita IMA dengan DM. Penelitian ini menggunakan desain cross sectional dengan melihat data rekam medis 2006-2011 di rumah sakit. Pada 70 kasus yang didiagnosis IMA dengan DM hanya terdapat 38 kasus yang memenuhi kriteria inklusi dan eklusi. Pasien dibagi dua kelompok berdasarkan kadar glukosa darah saat masuk yaitu Grup 1 (<200 mg/dL) dan Grup 2 (> 200 mg/dL). Outcome pasien juga dilihat dan dibagi menjadi 2 kelompok yaitu baik dan meninggal. Hasil analisis Chi square menunjukkan tidak ada hubungan yang signifikan antara kadar glukosa darah dengan kematian pada penderita IMA dengan DM (p = 0.653, PR=2.4, CI=0.354-16.258). Pemeriksaan laboratorium juga tidak menunjukkan perbedaan rata-rata yang signifikan kecuali pemeriksaan leukosit. Disimpulkan kadar glukosa darah tidak berhubungan dengan kematian sehingga tidak dapat digunakan sebagai faktor prediktor kematian pada penderita IMA dengan DM. Kata kunci: infark miokard akut, diabetes melitus, glukosa darah, kematian Acute Myocardial Infarction (AMI) is the the first cause of mortality in Indonesia. The incidence of AMI depends on the risk of atheroschlerosis, one of them is diabetes melitus (DM). In DM’s patient, blood glucose level will increase and some research reported the correlation of glucose abnormal with mortality, but it’s not clear. The research aims to know correlation of blood glucose level to mortality in acute myocardial infarction with diabetes melitus patients. This research used cross sectional design by looked at the medical record datas 2006-2011 in hospital. There are 70 cases which diagnosed as AMI with DM, but only 38 cases which fulfilled inclusion and exclusion criterias. Patients divided into 2 Grups depend on the blood glucose level on admission, Grup 1 (<200 mg/dL), Grup 2 (>200mg/dL). We also looked out the patient’s outcome and divided into 2 Grups, recovered and dead. This result by using Chi square analysis does not show a significant correlation between blood glucose level with death in AMI with DM patients (p = 0.653, PR=2.4, CI=0.354-16.258). Laboratory examination also did not show a significant difference of mean except for leucocyte count. The conclusion is blood glucose level has no correlation with death in AMI with DM, so it can’t used as predictor factor of death in patients AMI with DM.
Pengaruh Senam Ergonomis pada Penderita DM Tipe 2 terhadap Kadar Glukosa Darah Puasa dan Kadar Glukosa 2 Jam Postprandial Fahmi, Gusti Zidni; Widyatmoko, Agus
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 2 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v13i2.1065

Abstract

Pemantauan status metabolik penderita Diabetes Melitus (DM) merupakan hal yang penting dan sebagai bagian dari pengelolaan diabetes melitus. Latihan jasmani merupakan salah satu pilar dari keempat penatalaksanaan pada penderita DM. Salah satu bentuk latihan jasmani adalah senam ergonomis. Kemampuan seseorang untuk mengatur kadar  glukosa plasma agar tetap dalam batas-batas normal dapat ditentukan melalui tes kadar glukosa plasma puasa dan respons glukosa plasma terhadap pemberian glukosa (postprandial) . Penelitian ini bertujuan untuk mengetahui pengaruh senam ergonomis terhadap kadar glukosa darah, khususnya glukosa darah puasa dan 2 jam postprandial pada penderita DM tipe 2. Penelitian ini menggunakan metode cohort eksperimental dengan rancangan penelitian yang digunakan adalah randomized Control Group Pre test-Post test Design. Subyek penelitian adalah penderita DM tipe 2 yang berada di wilayah Tamantirto baik laki-laki maupun perempuan. Jumlah sampel sebanyak 30  responden yang diambil secara acak atau random yang memenuhi kriteria yang ditentukan oleh peneliti. Hasil penelitian diuji dengan uji paired t test,uji t test independent dan uji Chi Square dengan bantuan program computer SPSS versi 15.0. Hasil penelitian didapatkan p 0,005, yang berarti bahwa tidak ada perbedaan yang bermakna antara kelompok intervensi senam ergonomis dengan kelo The monitoring of metabolic status in Diabetes Melitus (DM) patients is the important thing and it becomes part of DM management. The exercise is one of the four pillars of DM management. One of the exercises is ergonomic gymnastics. Someone’s ability in control the plasma blood glucose level to remain in the normal limits could be determined with plasma blood glucose test and the response of plasma glucose to glucose load (Postprandial). This research is to know the influence of ergonomic gymnastics to the blood glucose level; especially fasting blood glucose and postprandial blood glucose in DM type 2 patients. This research is using cohort experimental method with randomized control group pre test-post test design. The subject of this research is the patients of DM type 2 in Tamantirto both man and woman. The number of sample is 30 respondents that were taken randomly and it is fulfill the criteria determined by the researcher. The result of this research is tested with paired t test, independent t test and Chi Square with SPSS computer program version 15.0. The result of this research is obtained p 0,005. It means there’s no significant difference between the intervention groups of ergonomic gymnastics and control group to the decrease of fasting blood glucose level and postprandial blood glucose.
Kadar Albumin dan Perbedaan Kualitas Hidup Penderita Gagal Ginjal Terminal Saat Menjalani Hemodialisis dan Setelah Pindah Ke Dialisis Peritoneal Mandiri Berkesinambungan di RS Dr. Sardjito Yogyakarta Widyatmoko, Agus
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 9, No 2 (2009)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v9i2.1596

Abstract

Plasma albumin levels may be a predictor of quality of life for people with end stage renal failure (ESRF). Objectives of this research is to know the difference quality of life patients with chronic renal failure after moving to self-sustaining peritoneal dialysis with albumin as seen through the HD. Research carried out by testing before and after the patient while undergoing HD, and after moving to the hemodialysis unit Dr. Sardjito Hospital Yogyakarta. Research conducted in the period from January 2005 until September 2005. As the inclusion criteria of chronic renal failure patients undergoing regular HD stable 2x a week and move to DPMB stable for 3 months. Albumin levels measured before the patient started DPMB. Quality of life ofpatients while undergoing HD and after undergoing DPMB measured by Short Form - 36. Statistical tests by using the paired t test with the level of p 0.05. Average albumin level 3.12 ± 0.59 g/dl. At the normal albumin group the mean score obtained at the time of mental health through the HD and 43.15 ± 11.3 after DPMB 67.1 ± 22.5 (p = 0.005). At the low albumin group the mean score obtained mental health when undergoing HD at 46.3 ± 8.1 and after undergoing DPMB 51.1 ± 10.5 (p = 0.054). At the normal albumin group obtained an average score of physical health when undergoing HD and 30.6 ± 15.1 after undergoing DPMB 32.3 ± 8.8 (p = 0.763). At the low albumin group obtained average scores of physical health when undergoing HD and 30.9 ± 9.9 after undergoing DPMB 34.1 ± 18.1 (p = 0.193). Obtained the overall mental health status (MCS) patients who undergo DPMB better than HD (p 0.001) It can concluded that ESRF patients with normal albumin levels had mental health status (MCS) is better when switching from HD to DPMB.Persepsi tentang kesehatan dan kualitas hidup penderita yang menjalani dialisis baik hemodialisis (HD) maupuan dialisis peritoneal mandiri berkesinambungan (DPMB) sangat bervariasi dan berpengaruh terhadap kondisi psikis dan semangat hidupnya. Kadar albumin plasma dapat menjadi prediktor bagi kualitas hidup penderita GGT. Tujuan penelitian ini adalah untuk mengetahui perbedaan kualitas hidup (KH) penderita gagal ginjal kronik setelah pindah ke dialisis peritoneal mandiri berkesinambungan (DPMB) dengan melihat kadar albumin saat menjalani HD. Penelitian dilakukan dengan uji before and after pada penderita saat menjalani HD dan setelah pindah ke DPMB di unit hemodialisa RS Dr. Sardjito Yogyakarta. Penelitian dilakukan pada bulan Januari sampai September 2005. Sebagai kriteria inklusi yaitu penderita gagal ginjal kronik yang menjalani HD rutin stabil 2x seminggu dan berpindah ke DPMB yang stabil selama 3 bulan. Kadar albumin diukur sebelum penderita memulai DPMB. Kualitas hidup penderita saat menjalani HD dan setelah menjalani DPMB diukur dengan Short Form - 36. Uji statistik dengan mengunakan paired t test dengan tingkat kemaknaan bila p 0,05. Hasil penelitian menunjukkan rerata kadar albumin 3,12±0,59 g/dl. Pada kelompok kadar albumin normal didapatkan rerata skor mental health pada saat menjalani HD 43,15 ± 11,3 dan setelah menjalani DPMB 67,1 ± 22,5 (p=0,005). Pada kelompok albumin rendah didapatkan rerata skor mental health pada saat menjalani HD 46,3 ± 8,1 dan setelah menjalani DPMB 51,1 ± 10,5 (p=0,054). Pada kelompok kadar albumin normal didapatkan rerata skor physical health pada saat menjalani HD 30,6 ± 15,1 dan setelah menjalani DPMB 32,3 ± 8,8 (p=0,763). Pada kelompok albumin rendah didapatkan rerata skor physical health pada saat menjalani HD 30,9 ± 9,9 dan setelah menjalani DPMB 34,1 ± 18,1 (p=0,193). Secara keseluruhan didapatkan status mental health (MCS) penderita yang menjalani DPMB lebih baik daripada HD (p 0,001). Disimpulkan bahwa penderita GGT dengan kadar albumin normal memiliki status mental health (MCS) yang lebih baik saat berpindah dari HD ke DPMB.
Perbedaan Kadar Endotelin-1 pada Penderita Hipertensi Stadium 1, 2 dan Bukan Penderita Hipertensi Widyatmoko, Agus
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 10, No 2 (2010)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v10i2.1570

Abstract

Hypertension is a risk factor of cardiovascular disease. Endothelin-1 is a strong vasoconstrictor andrelatedwith angiotensin IIin the arteriapressure regulation. This regulation influence in pathologic condition such as hypertension, congestive heart failure and cronic renal failure. The aim of this study is to compare the levels of endothelin-1 between the patient of hypertension stage 1; stage 2 and no hypertension person. The design of this study is cross sectional. Subjects were resident in the territory of District Health Center 2 Mlati, Sleman, DIY, men and women, aged 18-75 years. Hypertension Stage 1, Stage 2 and no hypertension groups were determined according to Joint National Committee (JNC) 7 criteria. Research subjects used randomly with stratification, fasting at least 8 hours before blood samples were taken for examination to measure the level of endothelin-1. There are 43 patients with hypertension stage 1, 54 patients with hypertension stage 2 and 54 non hypertension person. The difference of endothelin-1 level among groups were analyzed by Anova. This research shows that the increasing levels of endothelin-1 is in a row with increasing of blood pressure in hypertension stage 1 and 2; and the increasing more clear and meaningful in the 50-75 year age of group.Hipertensi merupakan faktor risiko penyakit jantung. Endotelin-1 adalah vasokonstriktor kuat dan berkaitan dengan angiotensin II dalam pengaturan tekanan arteria. Pengaturan ini berpengaruh terhadap kondisi patologis seperti hipertensi, gagal jantung kongesti dan gagal ginjal kronis. Tujuan penelitian ini adalah ingin membandingkan kadar endotelin-1 antara penderita hipertensi stadium 1; stadium 2, dan bukan penderita hipertensi. Rancangan penelitian ini adalah cross sectional. Subjek adalah penduduk di wilayah Kabupaten Puskesmas 2 Mlati, Sleman, DIY, pria dan wanita, usia 18-75 tahun. Hipertensi stadium 1, stadium 2 dan bukan penderita hipertensi ditentukan menurut kriteria JNC 7. Subyek penelitian diambil secara acak dengan stratifikasi, puasa minimal 8 jam sebelum sampel darah diambil untuk pemeriksaan kadar endotelin-1. Ada 43 pasien dengan hipertensi stadium 1, 54 pasien dengan hipertensi stadium 2 dan 54 orang non hipertensi. Perbedaan kadar endotelin-1 antar kelompok dianalisis dengan Anova. Hasil penelitian ini menunjukkan bahwa semakin tinggi kadar endotelin-1 maka semakin meningkat tekanan darah pada hipertensi tahap 1 dan 2; dan peningkatan usia lebih jelas dan berarti pada kelompok usia 50-75 tahun.
Perbedaan Kadar Endotelin-1 pada Penderita Hipertensi Stadium 1, 2 dan Bukan Penderita Hipertensi Widyatmoko, Agus
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 10, No 2 (2010)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v10i2.1570

Abstract

Hypertension is a risk factor of cardiovascular disease. Endothelin-1 is a strong vasoconstrictor andrelatedwith angiotensin IIin the arteriapressure regulation. This regulation influence in pathologic condition such as hypertension, congestive heart failure and cronic renal failure. The aim of this study is to compare the levels of endothelin-1 between the patient of hypertension stage 1; stage 2 and no hypertension person. The design of this study is cross sectional. Subjects were resident in the territory of District Health Center 2 Mlati, Sleman, DIY, men and women, aged 18-75 years. Hypertension Stage 1, Stage 2 and no hypertension groups were determined according to Joint National Committee (JNC) 7 criteria. Research subjects used randomly with stratification, fasting at least 8 hours before blood samples were taken for examination to measure the level of endothelin-1. There are 43 patients with hypertension stage 1, 54 patients with hypertension stage 2 and 54 non hypertension person. The difference of endothelin-1 level among groups were analyzed by Anova. This research shows that the increasing levels of endothelin-1 is in a row with increasing of blood pressure in hypertension stage 1 and 2; and the increasing more clear and meaningful in the 50-75 year age of group.Hipertensi merupakan faktor risiko penyakit jantung. Endotelin-1 adalah vasokonstriktor kuat dan berkaitan dengan angiotensin II dalam pengaturan tekanan arteria. Pengaturan ini berpengaruh terhadap kondisi patologis seperti hipertensi, gagal jantung kongesti dan gagal ginjal kronis. Tujuan penelitian ini adalah ingin membandingkan kadar endotelin-1 antara penderita hipertensi stadium 1; stadium 2, dan bukan penderita hipertensi. Rancangan penelitian ini adalah cross sectional. Subjek adalah penduduk di wilayah Kabupaten Puskesmas 2 Mlati, Sleman, DIY, pria dan wanita, usia 18-75 tahun. Hipertensi stadium 1, stadium 2 dan bukan penderita hipertensi ditentukan menurut kriteria JNC 7. Subyek penelitian diambil secara acak dengan stratifikasi, puasa minimal 8 jam sebelum sampel darah diambil untuk pemeriksaan kadar endotelin-1. Ada 43 pasien dengan hipertensi stadium 1, 54 pasien dengan hipertensi stadium 2 dan 54 orang non hipertensi. Perbedaan kadar endotelin-1 antar kelompok dianalisis dengan Anova. Hasil penelitian ini menunjukkan bahwa semakin tinggi kadar endotelin-1 maka semakin meningkat tekanan darah pada hipertensi tahap 1 dan 2; dan peningkatan usia lebih jelas dan berarti pada kelompok usia 50-75 tahun.
X-Ray Scanning Reduce Soluble Active Molecules of Mesenchymal Stem Cells Putra, Agung; Pasongka, Zenitalia; Widyatmoko, Agus; Prasetio, Ardi; Dirja, Bayu Tirta
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.14

Abstract

Mesenchymal Stem Cells secrete various anti-inflammatory and regenerative SAMs-MSCs that possess immunomodulatory properties and may accelerate wound healing. As a potential agent for therapeutic, SAMs-MSCs should be stable in any condition, including under X-Ray Scanning. The previous study reveal that X-Ray Scanning may induce protein damage. However, the investigation regarding the stability of SAMs-MSCs under X-Ray Scanning is limited, thus this study aimed to investigate the stability of SAMs-MSCs after X-Ray Scanning. Mesenchymal Stem Cell medium was filtrated using the TFF method at 300 and 5 kDa filter cut off and sterilized using 0,1 um syringe. The SAMs-MSCs underwent X-ray Scanning using public Air Port X-Ray twice. The SAMs-MSCs concentration was measured using ELISA. T-test analysis was performed for the statistical analysis with P<0,05. This study revealed that X-ray scanning reduce the concentration of SAMs-MSC. A previous study found that x-ray irradiation may damage protein at 6–18 keV caused by the energy deposited by photoelectrons that are generated by the interaction of X-ray photons and the protein leading to photoelectron-induced damage.
The Effect of Hypoxia on the Soluble Molecules of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Widyatmoko, Agus; Alif, Iffan; Irawan, Risky Candra Satria; Handoyo, Frigi Eko; Sidiq, Husni Ahmad
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.24

Abstract

Background: Umbilical cord-derived stem cells (UC-MSCs) are essential cell sources for cell-based therapies in regenerative medicine. Hypoxia is a key element of the stem cell niche and crucial for the preservation of UC-MSCs properties. The normal growth conditions for UC-MSCs are under atmospheric oxygen concentrations of 20–21%. However, the administration of UC-MSCs in inflammatory conditions provides oxygen-deficient environments. Thus, treating UC-MSCs with low oxygen exposure provides them with more survival and recovery potential. Objective: In this study, we assessed the impact of hypoxia incubation for 12 h on the UC-MSCs proteome. Methods: UC-MSCs were isolated from UC patients regarding informed consent. At passage 5, in 80% confluent, UC-MSCs were incubated in 5% O2 for 12 h. The morphology of UC-MSCs was assessed using a microscope. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were analyzed using ELISA. Results: Hypoxic condition could change their morphology and enhance the cellular density compared to normoxic conditions in vitro. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were significantly increased after the hypoxic condition of UC-MSCs compared to normoxia. Conclusion: Our findings suggest that the hypoxic condition was able to induce survival capacity and soluble molecules secreted by UC-MSCs.
Therapeutic Role of MSC-Secretome in Type 2 Diabetic Models: Correlation between Improved HOMA-IR and Attenuated Pancreatic-Hepatic Structural Alterations Wulandari, Putri Karenina Amalia; Sari, Fikriya Novita; Ardianto, Okky; Widyatmoko, Agus
International Journal of Cell and Biomedical Science Vol 4 No 10 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i10.69

Abstract

Background: Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance, β-cell dysfunction, and chronic hyperglycemia leading to multiorgan complications. Conventional therapies primarily target glycemic control but often fail to prevent progressive pancreatic and hepatic injury. This study investigated the therapeutic potential of hypoxic mesenchymal stem cell (MSC) secretome in improving insulin resistance and restoring tissue integrity in T2DM rat models. Methods: Male Wistar rats were induced with T2DM using a high-fat diet followed by streptozotocin–nicotinamide administration and subsequently treated intraperitoneally with MSC-secretome for four weeks. Fasting blood glucose, serum insulin levels, and HOMA-IR index were assessed, followed by histopathological evaluation of hepatic and pancreatic tissues. Results: The results showed that T2DM was significantly associated with elevated insulin levels and HOMA-IR values compared to the normal group, confirming insulin resistance. Treatment with MSC-secretome markedly reduced both parameters (p < 0.001), suggesting improved insulin sensitivity. Histological analyses revealed substantial hepatic and pancreatic degeneration in untreated diabetic rats, characterized by hepatocellular vacuolization, steatosis, and islet necrosis. Conclusion: Conversely, MSC-secretome treatment demonstrated remarkable restoration of lobular architecture, reduced lipid accumulation, and regeneration of pancreatic islets. These reparative effects are attributed to the secretome’s bioactive components that regulate oxidative stress, inflammation, and cellular regeneration. In conclusion, hypoxic MSC-secretome administration effectively ameliorates insulin resistance and attenuates hepatic and pancreatic damage in T2DM rats, underscoring its potential as a novel non-cell-based therapeutic strategy for metabolic disorders.
Co-Authors Agung Putra Alif, Iffan Ardianto, Okky Dirja, Bayu Tirta Gusti Zidni Fahmi, Gusti Zidni Handoyo, Frigi Eko Irawan, Risky Candra Satria Pasongka, Zenitalia Prasetio, Ardi Rina Puspita Sari Sari, Fikriya Novita Sidiq, Husni Ahmad Wulandari, Putri Karenina Amalia