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All Journal Journal of Science and Technology Research for Pharmacy Life Science Tadwin: Jurnal Ilmu Perpustakaan dan Informasi Jurnal Administrasi Karya Dharma International Journal of Cell and Biomedical Science
Prasetio, Ardi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences

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The Role of Mesenchymal Stem Cell in Controlling Liver Function on Liver Fibrosis Antari, Arini Dewi; Prasetio, Ardi; Nugroho, Baskoro Adi
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.45699

Abstract

ABSTRACT Background: Liver fibrosis (LF) is the end-stage of liver damage characterized by the increase concentration of SGOT and SGPT. A previous study, revealed that liver transplantation as the most effective therapy for LF has limited availability and remains a major challenge. Mesenchymal stem may control inflammation and regenerate damaged tissue leading to accelerated liver function repair. Aim: The purpose of this study was to determine the optimum doze for MSC administration to recover LF. Method: The research used variations doze of MSCs administration of 1x106 and 2x106. Liver firbosis was induced by twice a week intraperitoneal injection of carbon tetrachloride (CCl4) (Sigma-Aldrich) dissolved with olive oil in 1:1 ratio for eight weeks. The concentration of SGPT SGOT was measured at day 9 post MSC administration. One Way Anova was performed to analyze statictical data. Result: This study showed that there was a significant decrease in SGOT concentration (P < 0,05). P2 group treatment showed the lowest concentration of SGOT among the treatment (Figure B). In the same time, the administration of UC-MSCs reduce SGPT concentration insignificantly (P > 0,05). Conclusion: The transplantation of MSCs ameliorated LF by reducing SGPT and SGOT concentration
KEANEKARAGAMAN JENIS TIKUS DAN CECURUT DI GUNUNG UNGARAN JAWA TENGAH Prasetio, Ardi; Setiati, Ning
Life Science Vol 4 No 1 (2015)
Publisher : Universitas Negeri Semarang

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Abstract

Tikus dan cecurut memiliki peran yang penting dalam ekosistem. Gunung Ungaran sebagai salah satu habitat alami tikus dan cecurut mulai terganggu. Data mengenai tikus dan cecurut di Gunung Ungaran belum ada. Tujuan dari penelitian ini adalah untuk mengetahui tingkat keanekaragaman jenis tikus dan cecurut yang ada di Kawasan Gunung Ungaran. Lokasi pengambilan berada di hutan primer, kebun teh serta area perbatasan antara hutan primer dan kebun teh. Metode yang digunakan adalah Single Capture Live Trap yang dipasang pada transek. Berdasarkan hasil penelitian didapatkan lima jenis tikus dan dua jenis cecurut. Keanekaragaman jenis tikus paling tinggi berada di area perbatasan antara kebun teh dan hutan primer, sedangkan keanekaragaman jenis cecuru paling tinggi berada di area kebun teh Rats and shrews have important role in ecosystem. Mount Ungaran as one of natural habitat for rats and shrews is being disturbed. There is no sufficient data about rats and shrews diversity in Mount Ungaran. This research aim to know rats and shrews diversity in Mount Ungaran. This research is conducted in three area, primary forest, tea plantation, and the border between primary forest and tea plantation. Single Capture Live Trap is setted in a transect to collect data. The result show that there are five species of rats and two species of shwrews live in Mount Ungaran. The highest rats diversity is occured in the border between primary forest and tea plantation beside highest shrews diversity is occured in tea plantation
Dampak Kecerdasan Buatan (Artificial Intelligence) Terhadap Pemustaka Dalam Mencari Informasi di UPT Perpustakaan Universitas Islam Negeri Raden Fatah Prasetio, Ardi; Winanda, Tri
Jurnal Tadwin Vol 4 No 2 (2023): Tadwin: Jurnal Ilmu Perpustakaan dan Informasi
Publisher : Prodi Ilmu Perpustakaan dan Informasi Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/tadwin.v4i2.20059

Abstract

A library is an organizational unit aimed at collecting, storing, managing, and organizing a structured collection of library materials to be utilized by users as a source of information and as an enjoyable learning facility. Users who frequently use artificial intelligence will become dependent on it, but artificial intelligence can also assist them in easily finding the information they seek. This research employs a literature review method by searching for materials in terms of literature through journals, books, and magazines by exploring the internet and utilizing the existing artificial intelligence in the library of UIN Raden Fatah Palembang. This study is conducted to ascertain the impact of artificial intelligence on both the library and users wherever they seek information. Thus, the aim of this research is to investigate the extent of the impact of artificial intelligence on library users at UIN Raden Fatah Palembang. The use of artificial intelligence greatly aids library users in seeking information, and time efficiency in finding what they desire is a primary factor in using artificial intelligence in the library. The relevance of information provided by artificial intelligence becomes a distinct preference for library users in searching for information, and the advancement of technology in current developments is an attraction for libraries in facilitating librarians in seeking information in the library.
X-Ray Scanning Reduce Soluble Active Molecules of Mesenchymal Stem Cells Putra, Agung; Pasongka, Zenitalia; Widyatmoko, Agus; Prasetio, Ardi; Dirja, Bayu Tirta
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.14

Abstract

Mesenchymal Stem Cells secrete various anti-inflammatory and regenerative SAMs-MSCs that possess immunomodulatory properties and may accelerate wound healing. As a potential agent for therapeutic, SAMs-MSCs should be stable in any condition, including under X-Ray Scanning. The previous study reveal that X-Ray Scanning may induce protein damage. However, the investigation regarding the stability of SAMs-MSCs under X-Ray Scanning is limited, thus this study aimed to investigate the stability of SAMs-MSCs after X-Ray Scanning. Mesenchymal Stem Cell medium was filtrated using the TFF method at 300 and 5 kDa filter cut off and sterilized using 0,1 um syringe. The SAMs-MSCs underwent X-ray Scanning using public Air Port X-Ray twice. The SAMs-MSCs concentration was measured using ELISA. T-test analysis was performed for the statistical analysis with P<0,05. This study revealed that X-ray scanning reduce the concentration of SAMs-MSC. A previous study found that x-ray irradiation may damage protein at 6–18 keV caused by the energy deposited by photoelectrons that are generated by the interaction of X-ray photons and the protein leading to photoelectron-induced damage.
Effect of Time Transport on Mesenchymal Stem Cell Surface Markers: Unveiling the Influence of Cellular Translocation on Cellular Phenotype Wicaksono, Hendi; Prasetio, Ardi; Risky Chandra Satria Irawan; Naufal Ardjivani Arifin
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i4.29

Abstract

Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their ability to differentiate into multiple cell types and promote tissue repair. However, the effect of transportation time on the surface markers of MSCs remains understudied. This study investigated the impact of transportation time on MSC surface markers, specifically CD73, CD90, CD105, and hematopoietic lineage markers. MSCs were isolated from human umbilical cords and cultured. Flow cytometry analysis confirmed the expression of MSC surface markers. The MSCs were then subjected to simulated transportation for different time periods ranging from 0 to 24 hours at 2-8oC. After transportation, flow cytometry was used to analyze the expression of surface markers. The results showed that prolonged transportation time led to a decrease in the expression levels of CD73, CD90, and CD105, which are important markers for maintaining MSC functionality. Additionally, there was an increase in hematopoietic lineage marker expression. These findings suggest that transportation time can compromise the therapeutic potential of MSCs. Further investigation is needed to understand the underlying mechanisms responsible for the observed changes in surface marker expression. Optimization strategies, such as improved temperature control and protective agents, should be considered to mitigate the negative effects of prolonged transportation time. In conclusion, this study highlights the importance of considering transportation time and its impact on MSC surface markers in cellular therapy protocols. Understanding and addressing these effects are crucial for ensuring the quality and effectiveness of MSC-based therapies.
Priming of Mesenchymal Stem Cells for Enhanced Interleukin-10 Secretion via Conditioned Medium from Lipopolysaccharide-Activated Peripheral Blood Mononuclear Cells Prasetio, Ardi; Syafitri, Luthfiana Mifta; Prabowo, Adam; Alif, Iffan; Nurichsan, Aldan
International Journal of Cell and Biomedical Science Vol 2 No 5 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i5.39

Abstract

Background: Mesenchymal stem cells (MSCs) are known for their immunomodulatory properties, particularly their ability to secrete anti-inflammatory cytokines such as interleukin-10 (IL-10). Enhancing the secretion of IL-10 by MSCs could have significant therapeutic potential for treating inflammatory diseases. Objective: This study aimed to prime the secretion of IL-10 by MSCs through the use of conditioned medium (CM) derived from lipopolysaccharide (LPS)-induced peripheral blood mononuclear cells (PBMCs). Methods: MSCs were isolated from Wharton’s Jelly and characterized using flow cytometry and differentiation assays. PBMCs were isolated from human blood samples and stimulated with LPS to produce a pro-inflammatory environment. The conditioned medium from these LPS-induced PBMCs was collected and add to MSCs culture medium in 5% and 7.5%. After 24h and 48h incubation, IL-10 secretion by MSCs was measured using an enzyme-linked immunosorbent assay (ELISA). Results: The results demonstrated that MSCs cultured in the conditioned medium from LPS-induced PBMCs showed a significant increase in IL-10 secretion compared to control conditions in 24h exposure, but not significantly different in 48h. Conclusion: The exposure of conditioned medium from LPS-induced PBMCs may effectively enhances the secretion of IL-10 by MSCs.
Comparison of Two Tangential Flow Filtration Methods in Isolating CD63+/CD9+ Mesenchymal Stem Cell Exosome Putra, Agung; Alif, Iffan; Prasetio, Ardi; Prawitasari, Salindri
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

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Abstract

Background: Extracellular vesicles, particularly CD63+/CD9+ Mesenchymal Stem Cell Exosome (MSC-Exo), have emerged as crucial mediators of intercellular communication and potential therapeutic agents, including regenerative medicine and immunomodulation. However, the precise isolation and purification of MSC exosomes pose critical challenges. Tangential Flow Filtration (TFF) has gained recognition as an efficient exosome isolation method, offering scalability and versatility. In this study, we address the pressing need for standardized exosome isolation methods by comparing two distinct TFF-based protocols for isolating CD63+/CD9+ MSC exosomes based on filter size pore order. Methods: MSC-Exo were conducted from the Stem Cell and Cancer Research Laboratory (SCCR Indonesia), which were then processed through TFF using different filter sizes and orders. There are two filtration methods compared, first, MSC-Exo was filtered with 1000-5-500-300-100-50-10-5 filter order. Second procedure, MSC-Exo was filtered using 1000-500-300-100-50-10-5 filter order. Result: Flow cytometry analysis revealed variations in the percentage of CD63+/CD9+ in the MSC-Exo based on filter order. The results indicate that the choice of filter order significantly influences the size range with the highest concentration of CD63+/CD9+ MSC-Exo. Conclusion: This research underscores the importance of optimizing TFF-based isolation methods for CD63+/CD9+ MSC exosomes, especially in the order of filter pore size.
Model Kebijakan Mixed Scanning Dalam Implementasi Kebijakan Pengembangan Pariwisata di Provinsi Banten yang Berkelanjutan Reviana, Maghfira Nur; Jumiati, Ipah Ema; Salsabilla, Diviani; Prasetio, Ardi
Jurnal Administrasi Karya Dharma Vol. 4 No. 1 (2025): Jurnal Administrasi Karya Dharma (Maret 2025)
Publisher : Sekolah Tinggi Ilmu Administrasi Karya Dharma

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Abstract

This study analyzes the implementation of tourism development policies in Banten Lama, Banten Province, using a mixed scanning model. Banten Lama, as a cultural heritage area, has great potential for historical and religious tourism. This research aims to evaluate the effectiveness of policy implementation and identify challenges faced. The research method used is descriptive analysis with primary data obtained through interviews and secondary data from documents and archives. The results show that although the strategic policy has been well formulated through the 2017 MoU and Serang City Regional Regulation No. 14/2014, its implementation in the field is more incremental and adaptive to various obstacles, such as inter-agency coordination, conflicts of interest, and human resource capacity. The mixed scanning model proved relevant in analyzing this complexity, allowing for strategy adjustments while sticking to long-term goals. The research recommends the establishment of a special management agency for Banten Lama, the preparation of standardized SOPs, empowerment of localcommunities, and strict enforcement of rules to realize sustainable tourism governance and optimization of Banten Lama's potential as a leading destination.
Co-Authors Agung Putra Agus Widyatmoko, Agus Alif, Iffan Antari, Arini Dewi Dirja, Bayu Tirta Hendi Wicaksono Ipah Ema Jumiati Naufal Ardjivani Arifin Ning Setiati Nugroho, Baskoro Adi Nurichsan, Aldan Pasongka, Zenitalia Prabowo, Adam Prawitasari, Salindri Reviana, Maghfira Nur Risky Chandra Satria Irawan Salsabilla, Diviani Syafitri, Luthfiana Mifta Winanda, Tri