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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Ilmu Dasar UNEJ e-Proceeding Jurnal SOLMA INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Jurnal Kesehatan Masyarakat
Moh Mirza Nuryady, Moh Mirza
Biology Department, Mathematic and Natural Sciences Faculty, Jember University
Humanities Control & Systems Engineering Education Environmental Science Health Professions Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Imunogenic Protein of Salivary Gland from Anopheles sundaicus Armiyanti, Yunita; Nuryady, Moh Mirza; Utomo, Sugeng Setyo; Sardjono, Teguh Wahju; Fitri, Loeki Enggar; Senjarini, Kartika
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Malaria is still a major problem for developing countries, including Indonesia. One approach to overcome this disease is prevention by vaccination. However, there is still no effective malaria vaccine that is applicable. The ideal malaria vaccine is a combination vaccine that can prevent the pre-erythrocytic cycle, the erythrocytic cycle and transmission process. Salivary vector-based vaccine has the potential to be developed as a malaria vaccine because it can prevent transmission process and also decrease the morbidity of the disease. Saliva from Anopheles contains vasomodulator and immunomodulatory components, that are required in the blood feeding process, but in the same time it could enhance the transmission of the malaria parasite. If the component in the salivary vector can increase pathogen infection, then vaccinating the host with its anti-substances can control the transmission of pathogens (Transmision Blocking Vaccine). Anopheles sundaicus is an important vector of malaria in coastal areas of Java, Bali, Sumatra, Kalimantan and West Nusa Tenggara islands. Repeated exposures of Salivary Gland Extract (SGE) from this vector have been proven to be able to decrease parasitemic rates in mouse model for malaria in our study. The objective of this research is to determine and localize the immunogenic protein from SGE of An. sundaicus as the first step for the characterization of its immunomodulatory component. Mosquito salivary gland protein profile of An.sundaicus was determine by SDS-PAGE. Determination of salivary glands immunogenic proteins was conducted by Western Blotting with IgG from people living from endemic area as primary antibody. Out of 15 bands appeared in SDS PAGE ranging from 24 kD to 138 kD, only two protein bands with  molecular weights of 68 and 37 kDa were the most immunogenic. Those immunogenic proteins were consistent recognized by pooled serum of people as well as by individual response. Keywords: malaria, saliva, vector, immunogenic protein, vaccine
Pendampingan Pengelolaan Laboratorium IPA SMP Muhammadiyah Kota Malang Untuk Memfasilitasi Keterampilan Proses Sains Siswa Permana, Tutut Indria; Nuryady, Moh Mirza; Ariesaka, Kiky Martha; Ganes, Tegar; Nazila, Farah
Lumbung Inovasi: Jurnal Pengabdian kepada Masyarakat Vol. 9 No. 2 (2024): June
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/linov.v9i2.1855

Abstract

Pengelolaan laboratorium IPA harus didayagunakan secara efektif dan efisien untuk mendukung kegiatan kurikuler secara optimal. Pengelolaan laboratorium IPA yang optimal dapat mendukung tercapainya keterampilan proses sains siswa ketika menggunakan laboratorium pada proses pembelajaran. Namun demikian, tidak semua sekolah sudah memiliki sumber daya yang memadai dan pengelolaan laboratorium IPA yang baik. Seperti halnya yang terjadi di beberapa SMP Muhammadiyah di kota Malang yang mengalami kendala dalam pengelolaan laboratorium IPA di sekolah, seperti belum adanya etiket dan label pada alat bahan, belum adanya Standart Operational Procedure setiap alat, dan juga penataan alat bahan yang tidak aturan. Agar mampu memfasilitasi siswa untuk dapat memiliki keterampilan proses sains, sekolah memang perlu melakukan pengelolaan laboratorium IPA secara optimal untuk mendukung kegiatan akademik maupun non akademik siswa. Pengelolaan laboratorium yang baik akan memudahkan proses pembelajaran laboratorium, sehingga kegiatan pembelajaran praktik dilaboratorium dapat lebih maksimal. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan pendampingan dalam pengelolaan laboratorium IPA di SMP Muhammadiyah kota Malang. Kegiatan pengelolaan laboratorium ditujukan pada pihak sekolah yang terdiri dari pejabat struktural dan guru mata pelajaran yang berkaitan dengan kegiatan praktikum dalam laboratorium IPA. Kegiatan pengabdian tersusun atas: 1) observasi kondisi laboratorium IPA yang ada di sekolah; 2) sosialisasi dan workshop tentang standar pengelolaan laboratoium IPA; dan 3) pelaksanaan perbaikan managemen pengelolaan laboratorium IPA. Hasil kegiatan pengabdian ini adalah adanya peningkatan pengetahuan guru dalam pengelolaan laboratorium yang sesuai standar dan telah dilakukan perbaikan managemen pengelolaan laboratorium dengan melengkapi SOP yang ada di laboratorium. Lebih lanjut lagi, di laboratorium setiap sekolah mitra telah terdapat poster tatacara praktikum, SOP, etiket dan labelling alat bahan. Dengan demikian kegiatan pengabdian ini akan mewujudkan pengelolaan laboratorium IPA yang memadai untuk mendukung kegiatan akademik dan non akademik siswa yang bertujuan untuk meningkatkan kualitas proses pembelajaran di SMP Muhammadiyah Malang. Assistance in Managing Science Laboratories at Muhammadiyah Junior High Schools in Malang City to Facilitate Students' Scientific Process Skills Abstract The management of science laboratories must be utilized effectively and efficiently to optimally support curricular activities. Optimal management of science laboratories can support the achievement of students' scientific process skills when using the laboratory in the learning process. However, not all schools have adequate resources and good science laboratory management. This is the case in several Muhammadiyah Junior High Schools in Malang city, which face challenges in managing their science laboratories, such as the lack of labels and etiquette on equipment and materials, the absence of Standard Operating Procedures for each piece of equipment, and the disorganized arrangement of equipment and materials. To facilitate students in acquiring scientific process skills, schools indeed need to manage their science laboratories optimally to support both academic and non-academic student activities. Good laboratory management will facilitate the laboratory learning process, making practical learning activities in the laboratory more effective. This community service activity aims to provide assistance in managing science laboratories at Muhammadiyah Junior High Schools in Malang city. The laboratory management activities are aimed at school personnel, including structural officials and subject teachers involved in laboratory practical activities. The community service activities consist of: 1) observing the current condition of science laboratories in the schools; 2) socialization and workshops on standard science laboratory management; and 3) implementing improvements in laboratory management. The results of this community service activity include an increase in teachers' knowledge in managing laboratories according to standards, and improvements in laboratory management by completing the existing SOPs in the laboratories. Furthermore, each partner school's laboratory now has posters on practical procedures, SOPs, etiquette, and labeling of equipment and materials. Thus, this community service activity will result in adequate science laboratory management to support both academic and non-academic student activities, aiming to improve the quality of the learning process at Muhammadiyah Junior High Schools in Malang city.
In Silico Analysis of Active Compounds from Allium tuberosum as Drug Candidate for Inhibitor DENV-3 Envelope Protein Nuryady, Moh Mirza; Juliana, Levina Imelda; Ariesaka, Kiky Martha
Jurnal ILMU DASAR Vol 25 No 2 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i2.36400

Abstract

Dengue fever has become a global health issue, the development of dengue vaccine has not yet been established. Medicinal plants are an ideal alternative for DENV infection drugs. The purpose of this study was to determine in silico the potential of active compounds from Allium tuberosum as envelope protein inhibitors of DENV-3. The method of this research is to do docking analysis of compounds with DENV-3 envelope protein and analysis of amino acid residues using MVD, pharmacokinetic analysis using SwissADME, toxicity analysis using ProTox-II. The best docking value for the potential activity to inhibit the receptor DENV-3 is the thymidine compound (RS: -81.1245 kcal/mol). The highest activity of thymidine is the most promising as a drug candidate, as evidenced by the toxicity analysis which is predicted to have non-carcinogenic, non-mutagenic, inactive properties against hepatotoxicity, cytotoxicity and immunotoxicity parameters, as well as pharmacokinetic analysis that fulfills 6 parameters of lipopolicity, molecular weight, polarity. , insolubility, insaturation, and flexibility which indicate the drug candidate of thymidine is safe for its bioavailability. The conclusion from the results of this study is that one compound has the ability as an antiviral, binding score with DENV-3 is good, and is safe in terms of pharmacokinetics and toxicity, namely thymidine compound.
Expression of gonadotropin-releasing hormone receptor type-II correlates with proliferation activity in tissue microarray of rare ovarian tumor Ariesaka, Kiky Martha; Kusumanto, Ardhanu; Zucha, Muhammad Ary; Anggorowati, Nungki; Wicaksono, Agil Wahyu; Yunus, Moch; Fanani, Erianto; Nuryady, Moh Mirza
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.6034

Abstract

Activation of Gonadotropin-Releasing Hormone type II Receptors (GnRHR-II) exhibits antiproliferative activity. GnRHR-II is not only expressed exclusively in the pituitary, but also in a variety of tumors. To date, the clinical relevance of GnRHR-II in ovarian tumors is unclear. In addition, there is a lack of literature addressing GnRHR-II in ovarian tumors, especially rare types. This study was conducted to investigare the correlation between GnRHR-II expression with clinicopathology and proliferative activity of rare ovarian tumors. The purpose of this study was an analytic observational study with a cross-sectional design that utilized 18 ovarian tumor samples on tissue microarray (TMA). The expression of GnRHR-II and Ki67 was assessed using immunohistochemical staining (IHC) and observed using the IHC profiler plugin ImageJ software to obtain their respective H-scores. The data was analyzed using independent t-test, ANOVA, Pearson's test, and Fisher's exact test based on data types. The value of p<0.05 was considered statistically significant. GnRHR-II is expressed in various forms in ovarian tumors, including extrapituitary expression. GnRHR-II expression was highest in the sex cord stromal tumor (SCST) group, 110.30 ± 23.89 (p<0.0001). In addition, there was also a significant difference between GnRHR-II expression with age (p<0.001) and the primary tumor (p<0.05), but not with tumor type (p=0.101). There is a correlation between GnRHR-II expression and proliferative activity (r=-0.043, p=0.866). Elevated GnRHR-II expression is significantly correlation with SCST, individuals over 40 years of age, and tumors confined to the ovary and it is correlates with lower proliferative activity, although this correlation is very weak.
Penyuluhan dan Pemeriksaan Kesehatan serta Pengobatan Gratis pada Masyarakat Desa Sindurejo Kecamatan Gedangan Kabupaten Malang untuk Skrining Penyakit Tidak Menular Ariesaka, Kiky Martha; Iftitahurroza, Hilma Tsurayya; Nuryady, Moh Mirza
Jurnal SOLMA Vol. 14 No. 1 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i1.15729

Abstract

Latar Belakang: Kesehatan merupakan aspek mendasar bagi produktivitas dan kualitas hidup manusia. Hingga saat ini, penyakit tidak menular (PTM) masih menjadi perhatian global, termasuk di Indonesia. PTM, seperti kardiovaskular, diabetes, dan kanker, menyebabkan lebih dari 70% kematian global, dengan tingkat kematian tertinggi di negara berkembang. Di Indonesia, prevalensi diabetes dan hipertensi meningkat, khususnya di wilayah pedesaan. Berdasarkan hasil wawancara dengan pemangku kebijakan setempat, Desa Sindurejo, Kab. Malang menghadapi tantangan deteksi dan penanganan PTM karena jarak yang signifikan  dari pusat layanan kesehatan. Kegiatan pengabdian masyarakat ini bertujuan untuk menjawab permasalahan deteksi PTM tersebut  dengan melakukan skrining dan penyuluhan kesehatan di Desa Sindurejo. Metode: Kegiatan ini melibatkan mitra yaitu kepala desa Sindurejo dengan target sasaran seluruh warga lansia di desa Sindurejo. Metode melibatkan pemeriksaan kesehatan yang terdiri atas pengukuran berat badan, tanda-tanda vital (tekanan darah, laju denyut nadi, frekuensi napas, dan suhu tubuh), dan pengukuran kadar gula darah sewaktu (GDS), asam urat dan kolesterol, serta penyuluhan dengan metode ceramah. Hasil: Hasil kegiatan yang dilaksanakan pada Januari 2024 di Desa Sindurejo menunjukkan antusiasme partisipasi warga, diikuti oleh total 46 orang lansia. Kegiatan skrining dan pemeriksaan gratis berhasil mendiagnosis PTM pada sebagian besar individu, sementara penyuluhan meningkatkan kesadaran akan pentingnya pencegahan PTM. Kesimpulan: Kesimpulannya, kegiatan ini memberikan dampak positif pada kesehatan masyarakat pedesaan dan mendukung upaya global mengurangi beban PTM melalui deteksi dini dan modifikasi gaya hidup sehat
Breeding Site Preferences and Resistance Status of Aedes aegypti in Malang City Nuryady, Moh Mirza; Purwanti, Elly; Permana, Tutut Indria; Ariesaka, Kiky Martha
Jurnal Kesehatan Masyarakat Vol. 21 No. 2 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i2.21666

Abstract

Dengue Hemorrhagic Fever (DHF) remains a significant health challenge in urban and semi-urban regions, including Malang City, Indonesia, where Aedes aegypti is the primary vector. This study aimed to identify the breeding site preferences of Ae. aegypti and assess its resistance to 0.8% malathion and 0.05% cypermethrin insecticides. Using a descriptive observational design, ovitraps were deployed in three districts to collect mosquito larvae and eggs, and interviews with residents provided additional data on breeding site preferences. Resistance tests followed WHO guidelines, with mortality rates analysed after insecticide exposure. Results indicated that Ae. aegypti larvae were predominantly found in bathroom water tanks (45%) and flower vases (35%). Resistance status revealed geographical variability: Ae. aegypti in District 1 were resistant to cypermethrin, while populations in Districts 2 and 3 were susceptible, with average status of Ae. Aegypti to cypermethrin is tolerant. For malathion, resistance was widespread, particularly in District 3, with average status of Ae. Aegypti to malathion is resistant. These findings suggest that the use of malathion for vector control in Malang is no longer effective, while cypermethrin remains viable under strict monitoring to prevent future resistance. This study underscores the need for targeted insecticide use and regular monitoring to optimize vector control strategies and minimize DHF transmission.
Bioinformatics study of the A222V substitution in SARS-CoV-2 Spike Protein Ariesaka, Kiky Martha; Nuryady, Moh Mirza; Rini, Ika Agus
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 16, No 2 (2025): December 2025
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v16i2.39242

Abstract

COVID-19, caused by SARS-CoV-2, has infected over 200 million people and caused 4.3 million deaths globally, with over 3.7 million cases and 110,000 deaths in Indonesia. SARS-CoV-2, a single-stranded RNA virus, has a genome encoding proteins such as Spike (S), Envelope (E), Membrane (M), and Nucleocapsid (N). The S protein, comprising S1 and S2 domains, facilitates ACE2 receptor binding and membrane fusion. This study examines the phylogenetic relationship of the A222V substitution in the S protein with various global isolates. We collected 25 complete SARS-CoV-2 sequences from the GISAID database, performed multiple sequence alignments using Clustal W in MEGA-X software, and constructed phylogenetic trees using the neighbor-joining method. The urgency of this paper lies in understanding the impact of the A222V mutation on the virus's infectivity and spread, which is crucial for developing targeted treatments and vaccines. The A222V variant, first identified in Spain in summer 2020, has spread rapidly across Europe, raising concerns about its potential to increase transmissibility or affect vaccine efficacy. The A222V variant likely affects protein conformation or stability rather than receptor binding or membrane fusion. Understanding these changes is essential as it can influence the virus's behavior and efficacy of public health interventions. The study found high mutation rates in the S gene with diverse point mutations. Phylogenetic analysis revealed that A222V isolates clustered closely with D614G variants. In conclusion, not all mutations result in amino acid changes, and A222V has minimal impact on ACE2 binding but may influence protein stability.
Co-Authors Agil Wahyu Wicaksono Ardhanu Kusumanto Elly Purwanti Fanani, Erianto Ganes, Tegar Iftitahurroza, Hilma Tsurayya Ika Agus Rini, Ika Agus Juliana, Levina Imelda Kartika Senjarini Kiky Martha Ariesaka Loeki Enggar Fitri Mochammad Yunus, Mochammad Nazila, Farah Nungki Anggorowati Sugeng Setyo Utomo, Sugeng Setyo Teguh Wahju Sardjono Tutut Indria Permana Yunita Armiyanti Zucha, Muhammad Ary