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All Journal IPTEK The Journal for Technology and Science Techno.Com: Jurnal Teknologi Informasi Biogenesis: Jurnal Ilmiah Biologi MPI (Media Pharmaceutica Indonesiana) UNEJ e-Proceeding AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat AGROMIX
Wina Dian Savitri, Wina Dian
Department of Biology, Faculty of Biotechnology, University of Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Journal : UNEJ e-Proceeding

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The Inhibitionof Bacterial Metalloenzymes and Fungal Protein Synthesis on Explants Surfaces by Sterilizing Agents Savitri, Wina Dian
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Surface sterilization is a very important key for reaching a successful explants introduction to the in vitro environment. However, to get the best protocol of explants surface sterilization is not easy. Each explants has its characteristics and the contaminants (which usually are bacteria and fungus) are vary depending on its source. In order to know the best sterilization protocol of Phaleria macrocarpa leaves, some sterilizing agents were tested. This experiment suggests that ethylene bis dithiocarbamate may eradicate fungal infection by metalloenzymes inhibition, which is showed by 91.7% survivals. In the other hand, streptomycin sulfate may eliminate bacterial infection by inhibition of protein synthesis, which is showed by 95.8% to 98.3% survivals depending on the exposure time. Keywords: ethylene bis dithiocarbamate, metalloenzymes, streptomycin sulfate, protein synthesis, sterilization
The Inhibitionof Bacterial Metalloenzymes and Fungal Protein Synthesis on Explants Surfaces by Sterilizing Agents Wina Dian Savitri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Surface sterilization is a very important key for reaching a successful explants introduction to the in vitro environment. However, to get the best protocol of explants surface sterilization is not easy. Each explants has its characteristics and the contaminants (which usually are bacteria and fungus) are vary depending on its source. In order to know the best sterilization protocol of Phaleria macrocarpa leaves, some sterilizing agents were tested. This experiment suggests that ethylene bis dithiocarbamate may eradicate fungal infection by metalloenzymes inhibition, which is showed by 91.7% survivals. In the other hand, streptomycin sulfate may eliminate bacterial infection by inhibition of protein synthesis, which is showed by 95.8% to 98.3% survivals depending on the exposure time. Keywords: ethylene bis dithiocarbamate, metalloenzymes, streptomycin sulfate, protein synthesis, sterilization
Co-Authors Agnes Natalia Wijaya Alvian Bintura Andri Rianawati Andri Rianawati, Andri Antonius, Yulanda Askitosari, Theresia Desy Bintura, Alvian Carlois Poernomo Ecinsia, Emi Emi Ecinsia Fenny Irawati Fenny Irawati Fenny Irawati Fenny Irawati Florentinus W.N. Florentinus W.N. Nurtyandi Johan Sukweenadhi Laurensius Dewa Senapati Leonardo William Michael Anthony Thongiratama Mohammad Farid Naufal Poernomo, Carlois Popy Hartatie Hardjo Popy Hartatie Hardjo Popy Hartatie Hardjo Senapati, Laurensius Dewa Siska Citra Dewi Solichul Huda Thongiratama, Michael Anthony Vincentius Riandaru Prasetyo Vincentius Riandaru Prasetyo Wijaya, Agnes Natalia William, Leonardo Windra Prayoga Windra Prayoga Yulanda Antonius