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POTENSI BAKTERI INDIGEN DALAM BIODEGRADASI AIR SUNGAI Fidiastuti, Hasminar Rachman
Saintifika Vol 16, No 1 (2014)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.829 KB)

Abstract

Sungai Badeg merupakan salah satu sungai kecil di kota Malang yang tercemar limbah pabrik kulit. Hasil pengukuran kualitas pada sampel air sungai Badeg menunjukkan kondisi yang tidak memenuhi baku mutu air yaitu TSS sebesar 400 ppm, BOD sebesar 331 mg/L, COD sebesar 544 mg/L, DO sebesar 3,6 mg/L dan kadar lemak sebesar 280 mg/L. Lemak merupakan salah satu bahan organik yang sulit untuk diuraikan secara alamiah. Biodegradasi diharapkan dapat menjadi solusi perbaikan kondisi perairan dengan cara memanfaatkan aktivitas bakteri indigen dengan karakteristik pendegradasi lemak. Kegiatan biodegradasi dilakukan dengan cara mengintroduksi bakteri indigen (yang telah diseleksi dari proses isolasi dan memiliki potensi pendegradasi paling tinggi diantara jenis isolat lain yang ditemukan) kemudian dikultur dan diinokulasikan kembali pada sampel air sehingga proses transformasi akan berlangsung lebih optimal. Biodegradasi dilakukan secara in vitro, dan menunjukkan perubahan yang signifikan terhadap perubahan kadar TSS menjadi 293,3 ppm, BOD sebesar 117,3 mg/L, COD sebesar 165,3 mg/L, DO sebesar 19 mg/L dan kadar lemak sebesar 0,02 mg/L. Biodegradasi dengan memanfaatkan bakteri indigen dapat menjadi alternatif dalam memperbaiki kualitas perairan.
POTENSI BAKTERI INDIGEN DALAM MENDEGRADASI LIMBAH CAIR PABRIK KULIT SECARA IN VITRO Fidiastuti, Hasminar Rachman; Suarsini, Endang
Bioeksperimen: Jurnal Penelitian Biologi Vol 3, No 1: Maret 2017
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v3i1.3665

Abstract

Biodegradasi dapat menjadi salah satu teknologi pengolahan air limbah yang aman dan berwawasan lingkungan. Secara alamiah, bakteri yang berpotensi dalam proses biodegradasi dapat diisolasi dari limbah itu sendiri. Bakteri inilah yang disebut sebagai bakteri indigen. Sungai Badheg di kota Malang merupakan salah satu sungai yang tercemar limbah pabrik kulit. Hasil uji kualitas air dari sampel menunjukkan kondisi yang tidak memenuhi baku mutu air, yaitu TSS sebesar 400 ppm, BOD sebesar 331 mg/L, COD sebesar 544 mg/L, DO sebesar 3,6 mg/L dan kadar lemak sebesar 280 mg/L. Limbah pabrik kulit memiliki karakteristik yang berbau tajam, berwarna hitam pekat, dan mengandung lemak dalam jumlah yang besar. Lemak diketahui sebagai salah satu bahan organik yang sulit untuk diuraikan secara alamiah. Peran bakteri indigen akan mengoptimalkan proses biodegradasi lemak yang terjadi. Kegiatan biodegradasi dilakukan dengan cara mengintroduksi bakteri indigen yang telah diseleksi dari proses isolasi dan memiliki potensi pendegradasi paling tinggi, kemudian dikultur dan diinokulasikan kembali pada sampel air, sehingga proses transformasi akan berlangsung lebih optimal. Hasil biodegradasi secara in vitro menggunakan bakteri indigen, menunjukkan perubahan TSS sebesar 293ppm, BOD 117 mg/L, COD sebesar 165 mg/L, DO sebesar 19 mg/L dan kadar lemak sebesar 0,02 mg/L. Biodegradasi merupakan cara yang efektif dalam mendegradasi bahan organik, terutama kadar lemak.
DEVELOPING MODUL OF MICROBIOLOGY SUBJECT THROUGH BIODEGRADATION BY USING THE POTENCIAL OF INDIGEN BACTERIA Fidiastuti, Hasminar Rachman; Rozhana, Kardiana Metha
Jurnal Pendidikan Biologi Indonesia Vol 2, No 2 (2016): JULY
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.259 KB) | DOI: 10.22219/jpbi.v2i2.3464

Abstract

Students in the study of science, need to pay attention to the nature of science. Science studied to develop the ability to think (minds on), skills (hands on) as well as the scientific attitude (heart on). Study Microbiology in Prodi TIP, University Tibhuwana Tunggadewi is about microorganisms. The writers conducted research related to environmental remediation efforts to harness the potential of bacteria indigen as an agent in the process of biodegradation. Research results are used as a reference in the preparation of the module. Through modules made based on the results of research, students are expected to develop 1) the ability to think that microorganisms can become agents in the process of rehabilitation of environmental pollution and can be isolated from the waste, develop 2) skills through practical biodegradation, and develop 3) attitudes scientific through concept which have been studied, in providing practical solutions to the problems of environmental pollution. The module aims to improve the efficiency and effectiveness of learning in the classroom either time, funds, facilities or personnel to obtain maximum results. Application of Microbiology learning which refers to the fusion module and science process skills through implementation of practical activities, is expected to be an alternative to creating a learning Microbiology which refers to aspects of the process, product and scientific attitude. The result is already done three stage preparation of teaching materials in the form of Microbiology module which refers to the model of research and development model of the 4-D (Thiagarajan, et al), includes define, design, and development. Results at the stage of defining the characteristics of students analysis, task analysis, analysis of the concept and analysis of learning objectives. At the design stage to do the design and materials development, followed by the development phase, ie measurement of the feasibility of the modules have been prepared. Results obtained to measure the feasibility of the module is the achievement level of 85% by the expert development of teaching materials, 87% by material experts and 84% by the practitioner. The average yield of legibility student questionnaire amounted to 86.55%. The results of this validation consulted in order to repair the module. Keywords: Biodegradation in vitro, tanning pollutants, indigen bacteri
ISOLASI DAN KARAKTERISASI BAKTERI INDIGEN PENGURAI LEMAK PADA LIMBAH CAIR BATIK TULUNGAGUNG Fidiastuti, Hasminar Rachman; Lathifah, Anis Samroatul; Amin, Mohamad; Utomo, Yudhi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10430

Abstract

The native bacteria have a wide life habitat and have an important role as agents in the bioremediation process of waste. Batik as one of the products of Indonesian character, has become a contributor of liquid waste that can reduce water quality. Besides being rich in dyes, batik waste is also rich in fat content. The fat content in batik waste comes from the processing (coating the fabric with a layer of wax) and washing is done repeatedly. Fat has properties that are not soluble in water and is an organic material, which if high concentrations can reduce water quality. This study aims to isolate, identify, and measure the ability of indigenous bacteria to reduce fat content in liquid waste in vitro. The method used is experimental research. Isolation and culture using Busnall Hass media enriched with 1% olive oil, then proceed with a series of biochemical tests for the purpose of characterizing indigenous bacterial isolates. The results showed that Bacillus subtilis and Pseudomonas fluorescens have the potential to reduce the fat content of batik liquid waste.
Analisis Gaya Belajar terhadap Hasil Belajar Mahasiswa Angkatan Pertama Program Studi Pendidikan Biologi Universitas Tribhuwana Tunggadewi Lathifah, Anis Samrotul; Fidiastuti, Hasminar Rachman
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

The study program of Biology education in Unitri (University of Tribhuwana Tunggadewi, Malang) is a new study program, which has been operated for 1 year. Biology education has a vision that is a pioneer of biological educators who are based on cultured scientific as well as a center for the development of biology learning formed on multicultural and populist. To become a biology educator in accordance with the vision, the students are expected to understand the behavior or learning style to avoid the stress of learning that affects the continuity of the lecture. Students of Unitri who are mostly from Eastern Indonesia tend to be more vulnerable from learning stress since they have to adapt to different environments. Therefore, a research about student learning style analysis and its influence to learning result is conducted. De Porter and Hernacki divide learning styles into three types: visual, auditory, and kinesthetic learning styles. Based on the results of the learning style questionnaire given to 47 new students of biology education, 17% of students have visual learning style, 34% is auditory, and 49% is kinesthetic. To determine the effect on learning outcomes, the eta correlation test with significance value of learning result (score from the midterm test of cell biology course) is 0.086 bigger than α (0.05) which means there is no significant relationship between learning style of area with the score from the midterm test of cell biology course. This insignificant relationship can be influenced because students are new students who have not realized and understood their learning style. Hence, that special treatment is needed such as the existence of teaching materials or learning media that accommodate the learning style in order tp obtain significant results on the learning outcomes.
VALIDITAS LEMBAR KERJA PESERTA DIDIK INKUIRI TERBIMBING PADA MATERI SISTEM EKSKRESI MANUSIA Mitasari, Zuni; Lamboya, Apliana; Fidiastuti, Hasminar Rachman
Inteligensi : Jurnal Ilmu Pendidikan Vol 6, No 2 (2023)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/ilg.v6i2.4535

Abstract

This research aims to determine the validity of the guided inquiry-based student worksheet on human excretory system material at junior high school level. This type of this research, namely development research using the modified ADDIE model. The research was conducted at SMP Negeri 16 Malang.  The data collection instruments include (1) LKPD validation questionnaire, which consists of material expert and media expert validation questionnaires, (2) user expert questionnaires, in this case teachers, and (3) student response questionnaires. Based on the assessment of material experts, they obtained a score of 80.9% (valid), media experts scored 96.3% (valid), teacher user experts scored 83.5% (valid), and small-scale student response scores were 70% (good), and large scale 68% (Good). The inquiry LKPD product is valid and can be continued with quasi-experiments to determine the effect of using the product on the results and learning process.
Improving Student’s Motivation and Learning Outcomes Through Genetics E-Module Fidiastuti, Hasminar Rachman; Lathifah, Anis Samrotul; Amin, Mohamad; Utomo, Yudhi; Aldya, Riantina Fitra
Jurnal Ilmiah Peuradeun Vol. 9 No. 1 (2021): Jurnal Ilmiah Peuradeun
Publisher : SCAD Independent

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26811/peuradeun.v9i1.477

Abstract

The purpose of this study was to know the impact of the Genetics E-module to improve students’ motivation and learning outcomes during the learning process. The method applied was Research and Development, limited until the implementation stage, using the quasi-experiment method with one group pretest-posttest. The motivation instruments were modified questionnaire by Keller with ARCS model, consist of 4 aspects including attention, relevance, confidence, and satisfaction. The population were all of the students from class of 2017 in Biology Education department, Tribhuwana Tunggadewi State University. The pretest was held to know students’ motivation and learning outcomes when using a handout from the lecturer, while the posttest was to know students’ motivation and learning outcomes when using Genetics E-module. Data were collected from the motivation questionnaire and test. After using E-module, there were 27 students with high (71,91 ≤ x < 86), 18 students with medium (57,82 ≤ x < 71,91) and 5 students with low criterion (43,74 ≥ x) of motivation, and there were 58% students who passing the grade in Genetics test. Based on the data analysis, E-module was feasible to be used to improve students’ motivation and also learning outcomes in the Biology Education department.
EFEKTIVITAS NATURAL FACE MASK DALAM MENINGKATKAN KELEMBABAN KULIT WAJAH Masluhiya AF, Swaidatul; Fidiastuti, Hasminar Rachman
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 7, No 3 (2019): EDITION NOVEMBER 2019
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.686 KB) | DOI: 10.33366/jc.v7i3.1389

Abstract

This study aimed to determine the effect of using natural masks to moisturize dry skin. This natural mask was made of ingredients that are safe to use and based on special formulations which include; bengkoang powder (2 grams), chocolate powder (1 gram), black cumin oil (0.6 ml), and honey (5 ml). This formulation was made so that the resultied mask formed paste stucture that can be directly applied. Masks were given to ten probandal women who have dry skin types and are 30-40 years old. Masks were used twice / week for six weeks (12 times usage). The instrument used to measure the moisture level of facial skin was a skin analyzer (HL-611) by directly attaching it to the face area and the results can be seen after 4-5 seconds. The data were analysed using ANOVA test 95% confidence interval and significance test using SPSS 15 for windows. The results showed that skin moisture values increased around 3.9% within 6 weeks of use, with a 0.8% increase at week 2, at week 4 there was an increase of 1.65% and at week 6 there was an increase in skin moisture by 1.4%. The moisture value of the skin on the face area (forehead, nose, chin, cheeks) showed no significant difference with almost the same value of humidity. It is recommended for dry skin types to use a face mask that contains oil as a skin lubricant and to protect water evaporation from the skin. It is necessary to do further testing on this natural mask so that safety on the skin can be guaranteed. It needs to be done so that the final product obtained meets the face mask safety quality standards set by the government. Keywords: Bengkuang; cocoa; black cumin; honey; natural mask.
Kajian Biodegradasi secara In Vitro Menggunakan Bakteri Indigen Lipolitik Fidiastuti, Hasminar Rachman; Prasetiyo, Nugroho Aji
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Aktivitas industri penyamakan dan pengolahan kulit di daerah Kasin, Malang menyumbangkan polutan bagi perairan sungai Badeg. Hasil pengukuran kualitas pada sampel air sungai Badeg menunjukkan kondisi yang tidak memenuhi baku mutu air yaitu TSS sebesar 400 ppm, BOD sebesar 331 mg/L, COD sebesar 544 mg/L, DO sebesar 3,6 mg/L dan kadar lemak sebesar 280 mg/L. Limbah pabrik kulit mengandung lemak dan lemak merupakan salah satu bahan organik yang sulit untuk diuraikan secara alamiah. Biodegradasi  diharapkan dapat menjadi solusi perbaikan kondisi perairan dengan cara memanfaatkan aktivitas bakteri indigen dengan karakteristik pendegradasi lemak. Kegiatan biodegradasi dilakukan dengan cara mengintroduksi bakteri indigen yang telah diseleksi dari proses isolasi dan memiliki potensi pendegradasi paling tinggi, kemudian dikultur dan diinokulasikan kembali pada sampel air, sehingga proses transformasi akan berlangsung lebih optimal. Hasil biodegradasi secara in vitro menggunakan bakteri indigen, menunjukkan perubahan TSS sebesar 293ppm, BOD 117 mg/L, COD sebesar 165 mg/L, DO sebesar 19 mg/L dan kadar lemak sebesar 0,02 mg/L. Biodegradasi merupakan cara yang efektif dalam mendegradasi bahan organik, terutama kadar lemak.
Uji Karakteristik Limbah Cair Industri Batik Tulungagung: Penelitian Pendahuluan Fidiastuti, Hasminar Rachman; Lathifah, Anis Samrotul
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2018: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.097 KB)

Abstract

Produksi batik di Indonesia telah tersebar di 20 provinsi di Indonesia. UNESCO bahkan mengukuhkan batik sebagai warisan budaya milik Indonesia. Industri batik menghasilkan karakteristik limbah yang berwarna pekat akibat tingginya konsentrasi kadar warna dari proses pencelupan warna pada pembuatan batik. Hal ini berakibat pada badan air sebagai tempat pembuangan limbah secara langsung. Komposisi zat warna yang digunakan dalam industri terdiri atas bahan kimia organikdan anorganik. Jenis zat warna yang digunakan dalam industri batik sebagai tempat pengambilan sampel adalah menggunakan naphtol dan indigosol. Limbah batik juga berpotensi mengandung senyawa logam berat yang berasal dari komposisi bahan pewarna. Semakin besar proses produksi berlangsung, maka kapasitas limbah yang dihasilkan semakin besar. Hal ini akan berdampak pada terganggunya keseimbangan pada lingkungan sekitar industri. Penelitian ini merupakan penelitian pendahuluan yang bertujuan untuk mengkaji karakteristik limbah cair batik di Tulungagung dengan mengukur kadar BOD, COD, DO, TSS, pH, kadar lemak, kadar zat warna dan kadar Cr6+. Pengujian kadar BOD diukur dengan menggunakan metode 5th day incubation, kadar COD diukur dengan menggunakan metode analisis Dichromate Oxidation, kadar DO diukur dengan metode analisis Dissolved Oxygen Before, kadar TSS diukur dengan metode analisis gravimetri, analisis lemak menggunakan metode Direct Acid Hydrolysis, analisis kadar warna menggunakan metode spectrofotometry dan analisis Cr6+ menggunakan metode analisisspektrofotometry. Hasil penelitian menunjukkan karakteristik limbah cair pabrik batik di atas baku mutu yang telah ditentukan oleh Keputusan Menteri Negara Lingkungan Hidup Nomor 51/menlh/10/1995. Kadar BOD menunjukkan 376, 633 mg/L, kadar COD sebesar 568 mg/L, kadar DO sebesar 2, 374 mg/L, kadar TSS sebesar 1180 mg/L, pH sebesar 5,8; kadar lemak sebesar 0,240%, dan kadar Cr 6+sebesar 2,361 mg/L. Pengambilan cuplikan hanya dilakukan satu kali pada tiga titik lokasi pengambilan, sehingga hasil penelitian tidak bisa mewakili kondisi musim yang berlaku. Pengambilan cuplikan dilakukan pada musim kemarau. Hasil penelitian hanya berlaku untuk waktu dan kondisi saat pengambilan cuplikan di kecamatan Kauman, Tulungagung.