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All Journal Buletin Peternakan Jurnal Ilmu Lingkungan Jurnal Ilmu Dasar Jurnal Ilmiah Aplikasi Isotop Radiasi AL KAUNIYAH Journal of Mathematical and Fundamental Sciences Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Pro-Life JURNAL BIOLOGI INDONESIA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)
Megga Ratnasari Pikoli
(Scopus ID: 55792776200) Syarif Hidayatullah State Islamic University Of Jakarta
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Veterinary Other

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Isolation and Characterization of Cellulolytic Bacteria During Natural Fermentation of Sweet Orange Peel Waste (Citrus sinensis) La Ode Sumarlin; Farida Ariyanti; Megga Ratnasari Pikoli; Anna Muawanah; Meyliana Wulandari
Al-Kauniyah: Jurnal Biologi Vol 15, No 2 (2022): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i2.23357

Abstract

Abstract Orange peel is one of organic waste which contains fibers, such as cellulose and hemicellulose utilized by cellulolytic microorganisms as growth media in the fermentation process. Cellulolytic microorganisms are widely used in many industries. This research will observe the profile of bacterial colonies, particularly cellulosic bacteria, during the fermentation of orange peels (Citrus sinensis). Fermentation was carried out during the research process; the bacteria were further isolated in Carboxymethyl Cellulose (CMC) media. The fermentation process was performed for 14 weeks where sampling on the first week was done every day for five days (H0–H4), while sampling from the 2nd to 14th weeks were conducted once a week (M2–M14). The isolation process was carried out in a Nutrient Agar medium with spreading method by calculating the Total Plate Count (TPC) of bacterial colonies and observing the macroscopic morphology of bacterial colonies. Bacterial counts are expressed in Colony Forming Units (CFU)/mL or viable count/mL. The identification of bacterial genus was based on the Bergey's Manual of Determinative Bacteriology. Bacterial isolation from the fermentation of sweet orange peel resulted in 20 isolates where 16 isolates were found to be cellulolytic bacteria through qualitative test in Carboxymethyl Cellulose (CMC) agar plate. The hypothetic genus of 16 bacterial isolates were Eubacterium, Cellulomonas, Microbacterium, Micrococcus, Planococcus, Pseudomonas, Azotobacter, Azomonas, Flavobacterium, Cytophaga, and Jonesia. Isolate F15 (Cytophaga and Azomonas) was found to dominate the growth, while other isolates grew alternately with lesser frequency. Hypothetic genus of bacteria actively involved in the process were cellulolytic bacteria, allowing the liquid of fermentation products to be possibly used in the application.AbstrakKulit jeruk merupakan salah satu limbah organik yang mengandung serat seperti selulosa dan hemiselulosa yang dapat dimanfaatkan oleh mikroorganisme selulolitik sebagai media pertumbuhan dalam proses fermentasi. Mikroorganisme selulolitik telah digunakan di banyak industri. Penelitian ini mengamati profil koloni bakteri selama proses fermentasi kulit jeruk terutama bakteri selulotik. Selama proses penelitian dilakukan proses fermentasi, lalu bakteri diisolasi menggunakan media Carboxyl Methyl Callulose (CMC). Proses fermentasi dilakukan selama 14 minggu dengan rincian sampling pada Minggu ke-1 dilakukan setiap hari selama 5 hari (H0–H4), sedangkan minggu ke-2 hingga 14 dilakukan setiap seminggu sekali (M2–M14). Proses isolasi dilakukan dalam medium Nutrient Agar dengan teknik sebar dengan perhitungan koloni Total Plate Count (TPC) dan pengamatan morfologi koloni bakteri secara makroskopis. Hasil perhitungan bakteri dinyatakan dalam Colony Forming Units (CFU)/mL atau viabel count/mL. Pendugaan genus bakteri berdasarkan Bergey's Manual of Determinative Bacteriology. Hasil isolasi bakteri dari fermentasi kulit jeruk manis adalah 20 isolat yang 16 di antaranya merupakan bakteri selulolitik melalui uji kualitatif pada media plat Carboxymethyl Cellulose (CMC). Genus hipotetik bakteri dari 16 isolat adalah Eubacterium, Cellulomonas, Microbacterium, Micrococcus, Planococcus, Pseudomonas, Azotobacter, Azomonas, Flavobacterium, Cytophaga, dan Jonesia. Isolat F15 (Cytophaga dan Azomonas) mendominasi pertumbuhan, sedangkan isolat lain tumbuh berselang seling dengan frekuensi yang lebih kecil. Genus bakteri hipotetik yang terlibat aktif adalah bakteri selulolitik sehingga cairan hasil fermentasi dapat digunakan dalam aplikasi.
ANALYSIS OF NEW PRIMER PAIR CANDIDATES OF rbcL GENE FOR IDENTIFICATION OF MICROALGAE SCENEDESMACEAE Megga Ratnasari Pikoli
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 2 (2023): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i2.7918

Abstract

Scenedesmaceae is one of the microalgae groups that has been widely studied as promising biodiesel feedstock. Its morphological identification is often confused by environmental changes, so it requires molecular identification as well. The current study aimed to obtain primer pair candidates that identify the Scenedesmaceae based on the rbcL gene. The research used bioinformatics tools, which harvested rbcL protein sequence data, performed multiple sequence alignments, and designed primers based on conserved and less-conserved regions. The best left and right primers selected based on sequence length, melting temperature, 3' end stability, number of hairpins, and self-dimers, were paired, and three candidates were obtained. The three pairs were examined based on melting temperature difference, number of hetero-dimers, length of amplified nucleotide product, number of hits, and number of genera captured from the GenBank. Sce-16 (F, 5'-TGGTCGTGCTGTTTATGAATGT-3' and 1_RL, 5'-TGCCAAACATGAATACCACCA-3'), which is back-translated according to Hariotina sp. (AOY36008.1), is the most preferred candidate compared to the other two pairs after discussing their advantages and disadvantages. In the future, the proposed primer candidate needs to be validated through in vitro amplification with some optimizations to eliminate potential weaknesses.
Characterization of Protease from Endophytic Fungi Fusarium sp. JE-DP4a Hastuty, Aerma; Herawati, Irma; Pikoli, Megga Ratnasari
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.27293

Abstract

Protease is a catalytic enzyme that catalyzes a proteolysis process and breaks down a protein into smaller polypeptides or amino acids. Endophytic fungi can produce bioactive compounds, one of which is protease enzymes. The papaya leaf endophytic fungi used in this study was Fusarium sp. strain JE-DP4a. This study was conducted to determine the characterization of protease from endophytic fungi Fusarium sp. strain JE-DP4a with variations in incubation temperature of 30, 37, and 44oC and pH variation of 5.5-8. Measurement of protease enzyme activity using a spectrophotometer UV-Vis at 280 nm. Based on the research results, the highest protease activity produced at a temperature of 44oC is 53.78 U/mL, and specific activity is 17.31 U/mg protein. Protease activity at pH 7 shows the highest was 46.72 U/mL.Thus it is ably concluded that the protease produced by Fusarium sp. strain JE-DP4a belongs to the neutral protease group.
Analisis Gen tufA Secara In Silico Untuk Primer Identifikasi Mikroalga Trebouxiophyceae Megga Ratnasari Pikoli; Mahsa Nuraini Syahda; Festy Auliyaur Rahmah; Suharti Suharti
Al-Kauniyah: Jurnal Biologi Vol 17, No 1 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i1.35458

Abstract

AbstrakPenelitian belakangan ini menunjukkan bahwa di antara mikroalga yang memiliki kandungan lipid tinggi untuk dimanfaatkan sebagai bahan baku biodiesel, termasuk ke dalam kelas Trebouxiophyceae.  Kesederhanaan sel dan bentuknya yang mudah berubah menjadikannya sulit diidentifikasi secara morfologis. Oleh karena itu, identifikasinya perlu didampingi dengan metode molekuler yang mengamplifikasi gen dengan polymerase chain reactions (PCR). Metode PCR membutuhkan primer yang membatasi area pada DNA yang akan diamplikasi. Gen yang berpotensi dijadikan penanda identifikasi adalah tufA karena memiliki urutan yang lestari. Penelitian ini bertujuan mengajukan primer berdasarkan gen tufA untuk identifikasi Trebouxiophyceae. Sekuen gen tufA dikumpulkan dari database, disejajarkan, dan diamati area yang lestari untuk diambil kandidat primer. Kemudian primer forward dan reverse dipasang-pasangkan sambil diperiksa untuk diperoleh kandidat dengan sifat-sifatnya terbaik. Ada 5 pasangan kandidat yang dihasilkan yang kemudian diperiksa spesifisitasnya dalam menjaring anggota genus dari Trebouxiophyceae, dan juga yang bukan Trebouxiophyceae (Chlorophyceae dan Ulvophyceae) sebagai pembanding. Pasangan primer yang terbaik diusulkan dari penelitian ini adalah pasangan primer tufA. Trebo1 yang terdiri atas primer forward 5’-GAAAGTGTTGCTGGTGATAATGTTGG-3’ dan reverse 5’-GGAGTATGTCGACCACCTTCTTC-3’ yang menjaring 75% Trebouxiophyceae di GenBank. Pasangan primer ini menjaring lebih banyak Trebouxiophyceae dibandingkan dengan primer tufA yang pernah dipublikasi, namun memerlukan optimasi kondisi PCR untuk meminimalkan potensi terjadinya struktur sekunder. Dengan demikian, area lestari pada gen tufA berpotensi dijadikan primer untuk identifikasi Trebouxiophyceae.AbstractRecent research showed that microalgae having high lipid content to be used as raw materials for biodiesel belong to the class Trebouxiophyceae.  The simplicity of the cell and its easily changing shape make it difficult to identify morphologically. Therefore, its identification needs to be accompanied by molecular methods that amplify genes with polymerase chain reactions (PCR). The gene that could potentially be used as an identification marker is tufA because it has a conserved sequence. This study aims to propose a primer pair based on the tufA gene for the identification of Trebouxiophyceae. The sequences of the tufA gene were collected from a database of Trebouxiophyceae, aligned, and observed in conserved areas for primer candidates. Then the primary forward and reverse are mounted while checking for the candidate with the best properties. Five candidate pairs were produced, which were then tested for their specificity to bring in members of the Trebouxiophyceae, as well as non-Trebouxiophyceae (Chlorophyceae and Ulvophyceae) as comparisons. The best proposed primary pairs from this study were the primer pair tufA.Trebo1 which consists of the forward 5’-GAAAGTGTTGCTGGTGATAATGTTGG-3’ and the reverse 5‘-GGAGTATGTCGACCACCTTCTTc-3’ that capture 75% of the Trebouxiophyceae in the GenBank. This primer pair contains more Trebouxiophyceae than any previously published tufA primer but requires optimization of PCR conditions to minimize the occurrence of secondary structures. Therefore, the conserved area in the tufA gene has the potential to be used as a primer for identifying Trebouxiophyceae.
Comparable Antifungal Activity of Binahong (Anredera cordifolia) Leaf and Stem Ethanol Extracts Against Colletotrichum gloeosporioides Isolated from Anthracnose-infected Curly Red Chili Iis Rohayati; Nur Imaniati Sumantri; Junaidi; Megga Ratnasari Pikoli
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 2 (2026): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i2.48752

Abstract

Colletotrichum gloeosporioides is one of the pathogens that causes anthracnose in curly red chili (Capsicum annuum L. var. Longum). Anthracnose in red chile is marked characterized by fruit damage and consequently reduced market value. Anredera cordifolia (binahong) contains various bioactive compounds with presumed antifungal activity. Most binahong studies focus on the leaves, whereas this study compared the antifungal activity ethanol extracts from the leaves and stems against Colletotrichum gloeosporioides isolated from anthracnose-infected curly red chili fruits. An experimental method was used by varying extract concentrations in the binahong stem and leaf treatment groups. Fungus from anthracnose-infected fruit was isolated, purified, and identified as Colletotrichum gloeosporioides. Binahong leaves and stems were extracted with 96% ethanol and tested in two sequential stages at various concentrations, both showing that the highest concentrations exhibited the greatest inhibitory activity with no significant difference between leaf and stem extracts (P >0.05). Therefore, the leaf and stem extracts of binahong have similar biofungicidal potential that increases proportionally with concentration, against Colletotrichum gloeosporioides from curly red chili fruit.
Co-Authors AERMA HASTUTY Amalia Sekar Kusumawardhani Anna Muawanah Armar Riliansyah Tamala Dea Indriani Astuti Diannisa Syahwa Rahma Fadila Eko Pudjadi Farah Muthia Zadfa Farhan Wahyu Febrian Farida Ariyanti Festy Auliyaur Rahmah Fuad Albani Hanny Hafiar herawati, irma Iis Rohayati Innes Gania Sahira Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Irawan Sugoro Jilan Hasanati Junaidi La Ode Sumarlin Mahsa Nuraini Syahda MARATUS SHOLIHAH Meyliana Wulandari Muhammad Afi Naufal Muhammad Fathur Rachman Muhammad Indra Maulana Rizki Nana Mulyana Novi Mulyawati, Novi Nur Imaniati Sumantri Nurul Akhirati Hasanah Pingkan Aditiawati PINGKAN ADITIAWATI Putri Permata Utari Andini Rima Suciyani Setyawati, Adi Puji Setyawati, Adi Puji Suharti Suharti Tri Retno Dyah Larasati, Tri Retno Dyah Wahidin Teguh Sasongko Zahra Yunisa