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MEDIA ALTERNATIF EKSTRAK UBI JALAR SEBAGAI MEDIA TUMBUH JAMUR Aspergillus sp dan Candida sp PADA SERUMEN TELINGA PETANI DI KELURAHAN DORPEDU KOTA TERNATE Mukhtasyam Zuchrullah; Erpi Nurdin; Rahmawati Puni
CELEBES BIODIVERSITAS : Jurnal Sains dan Pendidikan Biologi Vol 6, No 1 (2023): Science Biology, Education, Conservation, Ecology
Publisher : Universitas Patompo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51336/cb.v6i1.390

Fungal are plants that require habitat conditions and high humidity. So it is one of the causes of public health problems in Indonesia. Groups at high risk of fungal infection are farmers because one of them is in direct contact with warm and humid areas at work. Otomycosis is a fungal infection that often occurs in the outer ear. Predisposing factors that influence include high humidity, local trauma which is often caused by the habit of using cotton buds, iron hooks and hair strands, long-term use of steroids and a history of dermatomycosis. Otomycosis is one of the common symptoms that are often found in ENT clinics and its prevalence reaches 9% of all patients who show symptoms and signs of otitis externa with Candida Sp and Aspergillus as the fungal species obtained the most from the oscillates. This study aims to determine the presence of fungal growth in the ear serum of farmers in Dorpedu Village. The location of the sampling was conducted in the Dorpedu Village, Ternate City and the location of this research was carried out at the Integrated Laboratory of the Health Polytechnic of the Ministry of Health of Ternate, Department of Medical Laboratory Technology. The method used in this study is to examine the culture and followed by 10% KOH staining. This study used accidental sampling technique with a sample of 20 farmers' ear serumThe results obtained showed that from 20 samples of farmers' ear serum in Dorpedu Village examined, Candida albicans and Aspergillus sp.: Based on the results of the research conducted, it can be seen that from 20 samples of farmer ear serum in Dorpedu Village, it was found that in the sweet potato extract media there were 17 (85%) positive samples of C. albicans, 1 (5%)positive samples of Aspergillus sp. PDA 20 (100%) samples were positive for C. albicans, and 2 (10%) samples were negative on sweet potato extract media where no fungal colonies were found. Sweet potato extract media can be used as an alternative medium for fungal growth.Keywords: Fungal, ear cerumen swab, otomycosis

DETEKSI JAMUR DERMATOFIT DAN NON DERMATOFIT PADA TINEA UNGUIUM MENGGUNAKAN MEDIA ALTERNATIF SUKUN DEKSTROSA AGAR Erpi Nurdin; Mukhtasyam Zuchrullah; Nurul Izza M Achiruddin
JURNAL INOVASI PENDIDIKAN DAN SAINS Vol 4 No 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v4i1.1451

Abstrak: Indonesia merupakan negara dengan cuaca lembab dan panas, menggambarkan daerah ideal untuk perkembangan aneka mikroorganisme antara lain jamur. Jamur merupakan tumbuhan yang memerlukan kondisi habitat dan kelembaban yang tinggi. Sehingga merupakan salah satu penyebab masalah kesehatan masyarakat di Indonesia. Kelompok yang berisiko tinggi terkena infeksi jamur yaitu para petani karena selalu berkontak langsung dengan daerah yang hangat dan lembab saat bekerja. Onikomikosis merupakan penyakit kuku yang paling umum dan menyebabkan hampir 50% dari seluruh kelainan kuku. Onikomikosis hanya digunakan untuk infeksi nondermatofita, sedangkan yang disebabkan jamur dermatofita disebut tinea ungunium. Untuk melihat pertumbuhan jamur pada infeksi kuku dengan menggunakan media alternatif sukun dekstrosa agar. Jenis penelitian ini menggunakan teknik accidental sampling. Identifikasi sampel menggunakan Metode KOH 10% dengan jumlah sampel 20 kuku kaki petani. Hasil yang diperoleh menunjukan bahwa dari 20 sampel kuku pada petani ditemukan jamur non dermatofit sebanyak 9 sampel (45%) Candida albicans, 4 sampel (20%) Aspergillus niger , dan jamur dermatofit 7 sampel (35%) Trichophyton rubrum. Berdasarkan hasil penelitian deteksi jamur dermatofit dan non dermatofit pada tinea unguium menggunakan media alternatif sukun dekstrosa agar ditemukan spesies jamur non dermatofit berupa Candida albicans dan Aspergillus niger , serta jamur dermatofit Trichophyton rubrum. Kata Kunci : Tinea unguium, jamur, media alternatif

Bacterial Resistance Test to Antibiotics Through Throat Swab Specimens of Children with Acute Respiratory Tract Infections (ARIs) Nurdin, Erpi; Zuchrullah, Mukhtasyam; Halik, Sofyanti Abdul
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.479

Acute Respiratory Infections (ARIs) are contagious diseases that affect the respiratory tract and remain a leading cause of morbidity and mortality, particularly among children. The use of antibiotics without proper sensitivity testing can lead to bacterial resistance; therefore, research is needed to determine the effectiveness of commonly prescribed antibiotics. ARIs is one of the primary causes of morbidity and mortality, especially in children in developing countries. The irrational use of antibiotics or the absence of adequate sensitivity testing is one of the main factors contributing to the rise in bacterial resistance, which may reduce the effectiveness of treatment and increase the public health burden. Objective to determine the sensitivity levels of several types of antibiotics against bacteria that cause ARIs, obtained from throat swab specimens of children with ARIs in several community health centers (Puskesmas) under the Ternate City Health Office. This study is a descriptive research using a laboratory approach, applying the Kirby-Bauer method for antibiotic sensitivity testing against bacteria using Mueller Hinton Agar (MHA) media. Bacterial identification showed a predominance of Staphylococcus epidermidis (45%), followed by Streptococcus pneumoniae (35%), and Streptococcus pyogenes (20%). Sensitivity testing revealed that Streptococcus pneumoniae was most sensitive to gentamicin (100%), while Staphylococcus epidermidis showed high sensitivity to ciprofloxacin (100%). On the other hand, cefixime exhibited the highest level of resistance. Sensitivity testing is crucial before administering antibiotics to prevent resistance and ensure appropriate treatment for children with ARIs.

PEMANFAATAN MEDIA ALTERNATIF DALAM ISOLASI BAKTERI PADA URINE PENDERITA DIABETES MELLITUS DI WILAYAH DAERAH BINAAN KELURAHAN DUFA-DUFA KOTA TERNATE Nurdin, Erpi; Mukhtasyam Zuchrullah; Lewa, Irma B. Hi
Jurnal Abdi Insani Vol 12 No 9 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i9.2811

Background: Bacteriological examination, particularly bacterial culture, is a standard method for diagnosing infectious diseases, including in patients with Diabetes Mellitus (DM). However, the limited availability and high cost of semi-synthetic culture media pose significant challenges, especially in regions such as North Maluku, including Ternate City. These constraints have encouraged the exploration of alternative culture media, one of which utilizes tuna-based ingredients. This alternative is expected to be applicable for urine culture tests in DM patients and to enhance the technical competencies of laboratory personnel. Nevertheless, the utilization of tuna as a component of alternative media for microbiological diagnostic purposes remains suboptimal. Methods: The activity was conducted in the form of community outreach in Dufa-Dufa Subdistrict through lectures, presentations, discussions, and evaluations using pre-tests and post-tests. The materials delivered focused on diabetes mellitus and the use of alternative media in microbiological examinations. Results: The results indicated a significant improvement in participants understanding. Initially, 51% of participants had no knowledge of DM and laboratory testing, which decreased to 0% after the educational session. The percentage of participants with moderate understanding declined from 44% to 0%, while those with good understanding increased from 5% to 49%, and those with excellent understanding rose from 0% to 51%. Additionally, the results of urine culture tests on patients with diabetes mellitus using tuna-based media showed that 90% of participants showed bacterial growth and 10% showed no bacterial growth. Conclusion: This initiative demonstrates that locally sourced alternative media hold promise as a feasible solution for laboratory diagnostics. Furthermore, the activity effectively raised community awareness regarding the importance of early detection of potential infections in patients with diabetes mellitus. Keywords: Diabetes mellitus, Urine culture, Alternative media, Laboratory diagnostics

Analisis Leukosit Urine Dipstik dan Kultur Bakteri Pada Pasien Diabetes Mellitus di Kota Ternate Nurdin, Erpi; Zuchrullah, Mukhtasyam; Nasarudin, Sandi
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 8 No 1 (2024): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v8i1.735

Background: Diabetes mellitus sufferers with poor diabetes control will generally develop urinary tract infections. Methods: This is a descriptive study, namely to obtain a dipstick urine leukocyte picture and bacterial culture in diabetes mellitus patients in Ternate City. Results: The research results showed that there were 21 female respondents (66%) and 11 male respondents (34%), and the frequency of age in the late elderly category was more dominant, namely 13 people (41%). Based on the length of time they have suffered from diabetes mellitus, with >10 years, there are 20 people (63%). The frequency of blood glucose increasing based on Point of Care Testing (POCT) examination was 23 people (72%). The urine culture results of diabetes mellitus patients showed that the average urine bacteria number was 6,3.103 CFU/mL with the interpretation of possible urinary tract infection (UTI), namely 11 people (34%), and 6,0.101 CFU/mL with the interpretation of no UTI, being 21 people (66%). Conclusion: Based on the research results, it was concluded that 3 respondents (9%) had positive leukocyte values despite the possibility of an UTI, and 8 respondents (25%) had negative leukocyte values despite the possibility of an UTI. There were 11 respondents (34%) with increased blood glucose accompanied by the possibility of UTI, and 3 respondents (9%) with increased blood glucose accompanied by positive leukocyte values.

Deteksi Gen Resistensi Karbapenemase Dan Metallo-Β-Lactamase Pada Acinetobacter Baumannii Zuchrullah, Mukhtasyam; Nurdin, Erpi
Jurnal Media Analis Kesehatan Vol 15 No 1 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i1.631

Peningkatan resistensi terhadap antibiotik golongan karbapenem merupakan salah satu fenomena yang harus diwaspadai. Acinetobacter baumannii merupakan salah satu bakteri penyebab infeksi berat yang didapatkan di rumah sakit. Penelitian ini bertujuan untuk mendeteksi gen intrinsik dan gen yang membawa resistensi obat golongan karbapenem terhadap A. baumannii. Penelitian ini dilaksanakan di Laboratorium HUM-RC RSPTN Universitas Hasanuddin Makassar. Desain penelitian ini adalah deskriptif eksperimental laboratorium dengan jumlah sampel 50 isolat yang didapatkan dengan cara total sampling yang dikumpulkan dari RSUP Wahidin Sudirohusodo Makassar. Data yang dianalisis adalah hasil karakteristik isolat, uji sensitivitas dengan menggunakan Vitek-2, uji genotipe gen intrinsik OXA-51 dan blaIMP-1 dengan menggunakan Polymerase Chain Reaction. Hasil penelitian menunjukkan bahwa dari total 50 isolat A. baumannii, ditemukan paling banyak pada sampel sputum sejumlah 30 isolat (60%). Dari total 50 sampel uji sensitivitas antibiotik, isolat A. baumanni paling banyak resisten terhadap meropenem 14 isolat (28%) kemudian imipenem 9 isolat (18%), dan Doripenem 9 isolat (18%). Gen intrinsik OXA-51 ditemukan pada semua isolat sampel sedangkan hanya satu isolat dari A. baumannii yang mampu memproduksi enzim metallo-β-Lactamase (MBL) dan membawa gen blaIMP-1 yaitu pada sampel urine yang seluruhnya resisten terhadap ketiga golongan obat karbapenem. Kata Kunci : Acinetobacter baumannii, Karbapenem, MBL, Polymerase Chain Reaction

Evaluasi dan Hubungan Ketepatan Penggunaan Obat Anti Tuberkulosis Lini 1 terhadap Karakteristik Pasien Rawat Jalan di Rumah Sakit Umum Daerah X: Evaluation and Correlation of the Appropriateness of the Use of First-Line Anti-Tuberculosis Drugs to the Characteristics of Outpatients at X Regional General Hospital Musdar, Tamzil Azizi; Hasmar, Wahyu Nuraini; Azis, Muhammad Israwan; Zuchrullah, Mukhtasyam; Saputro, Syaifullah; Kurniati, Kurniati; Ihsan, Mifta Khaerati; Supardi, Nurjannah; Santi, Elvira; Asjur, Asti Vebriyanti
Jurnal Sains dan Kesehatan Vol. 7 No. 6 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i6.2552

Tuberculosis is an infectious disease that attacks the respiratory organs (lungs). This study aims to determine the characteristics of the use of first-line anti-tuberculosis drugs (OAT) and evaluate the use of OAT based on the 2019 Tuberculosis Management Guidelines and analysis of the relationship between patient characteristics and treatment outcomes. Data collection was retrospective, with a sample size of 91 Patient Medical Records. The results showed that the most common patient characteristics were male (57.1%), age 46-55 years (24.2%), weight 38-54 kg (53.8%), with working status (52.7%), without comorbidities (89%), new patient type (92.3%), type of OAT category 1 (100%), unsuccessful treatment results (54.9%). Evaluation of OAT use was 100% correct indication, correct patient, and correct drug. While the correct dose (97.8%), correct duration of treatment (41.8%). The relationship analysis obtained age factors (p = 0.04), duration of treatment (p = 0.001) have a significant relationship to treatment outcomes, while gender (p = 0.301) and the number of P.PK (p = 0.733) both do not have a significant relationship to treatment outcomes. Keywords: TB, Evaluation of OAT Use, X Hospital Abstrak Penyakit Tuberculosis merupakan penyakit infeksi menular yang menyerang organ pernafasan (paru-paru). Penelitian ini bertujuan untuk mengetahui karakteristik penggunaan obat anti tuberkulosis (OAT) lini 1 dan mengevaluasi penggunaan OAT berdasarkan Pedoman Tatalaksana Tuberkulosis tahun 2019 serta analisis hubungan antara karakteristik pasien terhadap hasil pengobatan. Pengumpulan data secara retrospektif, jumlah sampel sebanyak 91 Rekam Medik Pasien. Hasil penelitian menunjukkan karakteristik pasien terbanyak yaitu berjenis kelamin laki-laki (57,1%), usia 46-55 tahun (24,2%), berat badan 38-54 kg (53,8%), dengan status bekerja (52,7%), tanpa penyakit penyerta penyerta (89%), tipe pasien baru (92,3%), jenis OAT kategori 1 (100%), hasil pengobatan tidak berhasil (54,9%). Evaluasi penggunaan OAT 100 % tepat indikasi, tepat pasien, dan tepat obat. Sedangkan tepat dosis (97,8%), tepat lama pengobatan (41,8%). Analisis hubungan diperoleh faktor usia (p=0,04), lama pengobatan (p=0,001) memiliki hubungan yang signifikan terhadap hasil pengobatan, sedangkan jenis kelamin (p=0,301) dan banyaknya penyakit penyerta kronis (P.PK) (p=0,733) keduanya tidak memiliki hubungan signifikan terhadap hasil pengobatan. Kata Kunci: TBC, Evaluasi Penggunaan OAT, RSUD X

DETEKSI JAMUR DERMATOFIT DAN NON DERMATOFIT PADA TINEA UNGUIUM MENGGUNAKAN MEDIA ALTERNATIF SUKUN DEKSTROSA AGAR Erpi Nurdin; Mukhtasyam Zuchrullah; Nurul Izza M Achiruddin
Jurnal Inovasi Pendidikan dan Sains Vol 4 No 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v4i1.1451

Abstrak: Indonesia merupakan negara dengan cuaca lembab dan panas, menggambarkan daerah ideal untuk perkembangan aneka mikroorganisme antara lain jamur. Jamur merupakan tumbuhan yang memerlukan kondisi habitat dan kelembaban yang tinggi. Sehingga merupakan salah satu penyebab masalah kesehatan masyarakat di Indonesia. Kelompok yang berisiko tinggi terkena infeksi jamur yaitu para petani karena selalu berkontak langsung dengan daerah yang hangat dan lembab saat bekerja. Onikomikosis merupakan penyakit kuku yang paling umum dan menyebabkan hampir 50% dari seluruh kelainan kuku. Onikomikosis hanya digunakan untuk infeksi nondermatofita, sedangkan yang disebabkan jamur dermatofita disebut tinea ungunium. Untuk melihat pertumbuhan jamur pada infeksi kuku dengan menggunakan media alternatif sukun dekstrosa agar. Jenis penelitian ini menggunakan teknik accidental sampling. Identifikasi sampel menggunakan Metode KOH 10% dengan jumlah sampel 20 kuku kaki petani. Hasil yang diperoleh menunjukan bahwa dari 20 sampel kuku pada petani ditemukan jamur non dermatofit sebanyak 9 sampel (45%) Candida albicans, 4 sampel (20%) Aspergillus niger , dan jamur dermatofit 7 sampel (35%) Trichophyton rubrum. Berdasarkan hasil penelitian deteksi jamur dermatofit dan non dermatofit pada tinea unguium menggunakan media alternatif sukun dekstrosa agar ditemukan spesies jamur non dermatofit berupa Candida albicans dan Aspergillus niger , serta jamur dermatofit Trichophyton rubrum. Kata Kunci : Tinea unguium, jamur, media alternatif

BACTERIAL CULTURE PROFILE ON THROAT SWAP OF PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION AT KALUMPANG HEALTH CENTER TERNATE CITY Erpi Nurdin; Mukhtasyam Zuchrullah; Fifi B Umagapi
Jurnal Inovasi Pendidikan dan Sains Vol 4 No 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v4i3.1899

Abstract: Respiratory tract infection is a common disease in society, and is one of the highest causes of death in children under 5 years of age (22.30%). Acute respiratory tract infections are divided into lower respiratory tract infections and upper respiratory tract infections. This infectious disease can be carried out by bacteriological examination using selective media to see the bacterial species. The aim of the research was to determine the bacterial culture profile in throat swabs of patients with acute respiratory infections at the Kalumpang Community Health Center, Ternate City using selective media, biochemical tests and gram staining. Research conducted using descriptive methods. Based on data on the characteristics of the Acute Respiratory Infection (ARI) patients studied, there were 17 men (57%) and 13 women (43%), while the age group with the most sufferers was in the 1-10 year age range, namely 9 people (30%) and then in the age range >50 years, namely 8 people (27%). Meanwhile, the percentage of culture profile findings from the bacterial species Stapylococcus aureus was 50%, Streptococcus pygones 33%, Pseudomonas aeruginosa 7%, Corynebacterium diphtheriae 10%. Found 98% gram positive bacteria and 2% gram negative bacteria. From the research results, it can be concluded that the most common bacterial culture profile in the throat swabs of upper respiratory tract infection patients is gram-positive bacteria consisting of the species Stapylococcus aureus, Streptococcus pygones, and Corynebacterium diphtheriae. The gram negative bacterial profile obtained was Pseudomonas aeruginosa. Keywords : Acute Respiratory Infections, throat swab, culture, bacteria

Studi Pertumbuhan Jamur pada Media Alternatif Kacang Merah (Phaeseolus vulgaris) Erpi Nurdin; Windi Alicya Samantha; Miranda A. Abd Karim; Adetya Ardheva Septyana; Gevanda Adnilla Rizkika Putri; Mukhtasyam Zuchrullah
Jurnal Inovasi Pendidikan dan Sains Vol 5 No 3 (2024): December
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v5i3.2339

Media PDA (Potato Dextrose Agar) dan SGA (Sabouraud Glukosa Agar) yang memiliki pH rendah (4,5–5,6) sering digunakan untuk mendukung pertumbuhan jamur. Namun, ketersediaan media ini terbatas karena hanya tersedia dalam bentuk instan dari pabrik tertentu. Penelitian ini bertujuan mengembangkan media alternatif dari sumber nutrisi kacang merah (Phaseolus vulgaris) untuk menumbuhkan jamur. Penelitian menggunakan desain eksperimental dengan pendekatan cross-sectional, melibatkan sampel kuku penderita tinea unguium pada petani. Hasil menunjukkan bahwa rerata diameter koloni jamur pada media alternatif ekstrak kacang merah adalah 4,49 mm, media alternatif powder kacang merah 4,35 mm, dan media PDA 2,2 mm. Jumlah koloni jamur pada media ekstrak kacang merah mencapai 32 koloni, sementara media powder kacang merah dan PDA masing-masing menghasilkan 7 koloni. Kesimpulannya, media alternatif berbasis kacang merah, baik dalam bentuk ekstrak maupun powder, dapat digunakan sebagai pengganti media PDA dengan efektivitas yang sangat baik untuk pertumbuhan jamur

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