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Hubungan Cemaran Bakteri Salmonella Typhi pada Feses Anak Terhadap Personal Higiene di Kelurahan Kampung Makassar Timur Kota Ternate erpi nurdin
Jurnal Kesehatan Vol 11 No 1 (2018): Jurnal Kesehatan Published By Poltekkes Ternate, Juni 2018
Publisher : UPPM Poltekkes Kemenkes Ternate

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.428 KB) | DOI: 10.32763/juke.v11i1.122

Abstract

Cemaran bakteri Salmonella typhi pada feses sangat erat kaitannya dengan hygiene pribadi. Penularan penyakit ini hamper selalu terjadi melalui makanan dan minuman yang terkontaminasi, yang menyebabkan penyakit abdominalis. Infeksi bakteri Salmonella typhi diperkirakan angka insidensi di seluruh dunia terdapat sekitar 17 juta per tahundengan 600.000 orang meninggal. WHO memperkirakan 70% kematianterjadi di Asia.Kampung Makassar merupakan kelurahan dengan sanitasi yang belum memadai. Penelitian ini bersifat komparatif dan bertujuan untuk mengetahui hubungan cemaran bakteri Salmonella typhi terhadap personal higiene anak di Kelurahan Kampung Makassar Kota Ternate.Teknik pengambilan sampel adalah secara non random dengan desain purposive sampling sebesar 40 orang. Sampel tersebut kemudian dibuat perlakuan untuk diidentifikasi Salmonella typhi dengan metode kultur feses. Dari hasil penelitian di peroleh hasil 17 sampel positif terdeteksi positif sebagai Salmonella typhi, dengan tingkat hygiene 3 buruk, 30 cukup baik , dan 7 baik. Berdasarkan hasil pengolahan data diperoleh p value 0,037<0,05. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat hubungan yang bermakna antara cemaran Salmonella typhi pada feses anak terhadap personal hygiene.
Spill Kit Infeksius berbahan Virgin Coconut Oil 71% Efektif sebagai Desinfektan Mikroba Urin penderita Infeksi Saluran Kemih: Penelitian Laboratorium Hi Lewa, Irma B; Nurdin, Erpi
Health Information : Jurnal Penelitian Vol 15 No 3 (2023): September-Desember
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36990/hijp.v15i3.1154

Abstract

Virgin coconut oil atau VCO merupakan salah satu hasil olahan buah kelapa (Cocos nucifera L.) yang mengandung asam lemak sehingga berpotensi memiliki antimikroba. Spill kit merupakan peralatan yang digunakan untuk membersihkan material yang berbahaya atau infeksius yang berbentuk cair. Namun, penggunaan cairan bahan kimia dari komponen spill kit secara terus menerus akan berdampak buruk pada lingkungan. Penelitian ini bertujuan untuk mengetahui efektivitas VCO sebagai bahan spill kit desinfektan. Jenis penelitian yang dilakukan adalan deskriptif dengan pendekatan eksperimental. Sampel yang digunakan adalah urin penderita infeksi saluran kemih pada Puskesmas. Perlakuan urin dengan pengkulturan pada media agar dengan jenis kultur urin, kultur kemudian diberikan perlakuan vco dan klorin. Berdasarkan pengamatan kultur pada spesimen yang dilanjutkan dengan pewarnaan bakteri diperoleh gram positif 40% dan terdapat gram negatif 60%. Rerata jumlah koloni pada sampel urin yaitu 42 koloni, VCO 12 koloni, dan klorin tidak teramati pertumbuhan koloni. Rerata angka kuman pada urin sebelum perlakuan yaitu 1,4.104 CFU/ml, setelah perlakuan penambahan clorin pada urin yaitu 3,3.101 CFU/ml dan setelah penambahan VCO pada urin yaitu 4,1.103 CFU/ml. Terdapat penurunan angka kuman 1,4.104 CFU/ml (100%) pada penambahan Klorin dan penurunan 9,9.103 CFU/ml (71%) pada penambahan VCO. Efektivitas VCO sebagai pengganti klorin untuk desinfektan pada pertumbuhan mikroba urin yakni 71% sedangkan Klorin 100%. perlu dilakukan penambahan waktu suspense VCO untuk mendapatkan hasil sebagai disinfektan yang lebih maksimal.
Profil Kecacingan dan Kadar Hemoglobin Pada Ibu Hamil Dalam Upaya Pencegahan Stunting di Puskesmas Wilayah Kota Ternate Puasa, Rony; Yulianingsih, Aan; Nurdin, Erpi; Rafika, Rafika
Jurnal Media Analis Kesehatan Vol 16 No 1 (2025): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v16i1.1127

Abstract

Masalah kesehatan ibu hamil merupakan isu penting dalam upaya meningkatkan kesejahteraan ibu dan bayi, serta mencegah berbagai komplikasi yang dapat mempengaruhi pertumbuhan dan perkembangan anak. Di Indonesia, stunting, yang ditandai dengan tinggi badan anak yang kurang dari standar usianya, masih menjadi tantangan utama dalam kesehatan masyarakat. Kecacingan pada ibu hamil dapat menyebabkan anemia karena kehilangan zat besi, dan anemia yang tidak ditangani dapat meningkatkan risiko bayi lahir dengan berat badan rendah atau stunting akibat kekurangan asupan nutrisi selama kehamilan. Infeksi cacing, terutama oleh cacing kremi, cacing gelang, dan cacing tambang, dapat mengganggu penyerapan nutrisi dan menyebabkan defisiensi zat besi, yang berdampak pada kadar hemoglobin. Tujuan penelitian ini untuk menggambarkan profil kecacaingan dan kadar Hb pada ibu hamil. Penelitian ini dilaksanakan di Laboratorium Poltekkes Kemenkes Ternate. Desain penelitian ini adalah deskriptif  dengan jumlah sampel sebanyak 98 responden yang diambil secara random sampling dari 8 Puskesmas kemudian diperiksa kadar Hb pada darah dan telur cacing pada feses responden. Hasil penelitian pada 98 responden ibu hamil tidak menunjukkan adanya telur cacing pada feses dan terdapat 46 responden yang mengalami anemia dengan anemia ringan sebanyak 30 responden (65,2%) dan anemia sedang sebanyak 16 responden (16,4%).
Bacterial Resistance Test to Antibiotics Through Throat Swab Specimens of Children with Acute Respiratory Tract Infections (ARIs) Nurdin, Erpi; Zuchrullah, Mukhtasyam; Halik, Sofyanti Abdul
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.479

Abstract

Acute Respiratory Infections (ARIs) are contagious diseases that affect the respiratory tract and remain a leading cause of morbidity and mortality, particularly among children. The use of antibiotics without proper sensitivity testing can lead to bacterial resistance; therefore, research is needed to determine the effectiveness of commonly prescribed antibiotics. ARIs is one of the primary causes of morbidity and mortality, especially in children in developing countries. The irrational use of antibiotics or the absence of adequate sensitivity testing is one of the main factors contributing to the rise in bacterial resistance, which may reduce the effectiveness of treatment and increase the public health burden. Objective to determine the sensitivity levels of several types of antibiotics against bacteria that cause ARIs, obtained from throat swab specimens of children with ARIs in several community health centers (Puskesmas) under the Ternate City Health Office. This study is a descriptive research using a laboratory approach, applying the Kirby-Bauer method for antibiotic sensitivity testing against bacteria using Mueller Hinton Agar (MHA) media. Bacterial identification showed a predominance of Staphylococcus epidermidis (45%), followed by Streptococcus pneumoniae (35%), and Streptococcus pyogenes (20%). Sensitivity testing revealed that Streptococcus pneumoniae was most sensitive to gentamicin (100%), while Staphylococcus epidermidis showed high sensitivity to ciprofloxacin (100%). On the other hand, cefixime exhibited the highest level of resistance. Sensitivity testing is crucial before administering antibiotics to prevent resistance and ensure appropriate treatment for children with ARIs.
PEMANFAATAN MEDIA ALTERNATIF DALAM ISOLASI BAKTERI PADA URINE PENDERITA DIABETES MELLITUS DI WILAYAH DAERAH BINAAN KELURAHAN DUFA-DUFA KOTA TERNATE Nurdin, Erpi; Mukhtasyam Zuchrullah; Lewa, Irma B. Hi
Jurnal Abdi Insani Vol 12 No 9 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i9.2811

Abstract

Background: Bacteriological examination, particularly bacterial culture, is a standard method for diagnosing infectious diseases, including in patients with Diabetes Mellitus (DM). However, the limited availability and high cost of semi-synthetic culture media pose significant challenges, especially in regions such as North Maluku, including Ternate City. These constraints have encouraged the exploration of alternative culture media, one of which utilizes tuna-based ingredients. This alternative is expected to be applicable for urine culture tests in DM patients and to enhance the technical competencies of laboratory personnel. Nevertheless, the utilization of tuna as a component of alternative media for microbiological diagnostic purposes remains suboptimal. Methods: The activity was conducted in the form of community outreach in Dufa-Dufa Subdistrict through lectures, presentations, discussions, and evaluations using pre-tests and post-tests. The materials delivered focused on diabetes mellitus and the use of alternative media in microbiological examinations. Results: The results indicated a significant improvement in participants understanding. Initially, 51% of participants had no knowledge of DM and laboratory testing, which decreased to 0% after the educational session. The percentage of participants with moderate understanding declined from 44% to 0%, while those with good understanding increased from 5% to 49%, and those with excellent understanding rose from 0% to 51%. Additionally, the results of urine culture tests on patients with diabetes mellitus using tuna-based media showed that 90% of participants showed bacterial growth and 10% showed no bacterial growth. Conclusion: This initiative demonstrates that locally sourced alternative media hold promise as a feasible solution for laboratory diagnostics. Furthermore, the activity effectively raised community awareness regarding the importance of early detection of potential infections in patients with diabetes mellitus. Keywords: Diabetes mellitus, Urine culture, Alternative media, Laboratory diagnostics
Analisis Leukosit Urine Dipstik dan Kultur Bakteri Pada Pasien Diabetes Mellitus di Kota Ternate Nurdin, Erpi; Zuchrullah, Mukhtasyam; Nasarudin, Sandi
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 8 No 1 (2024): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v8i1.735

Abstract

Background: Diabetes mellitus sufferers with poor diabetes control will generally develop urinary tract infections. Methods: This is a descriptive study, namely to obtain a dipstick urine leukocyte picture and bacterial culture in diabetes mellitus patients in Ternate City. Results: The research results showed that there were 21 female respondents (66%) and 11 male respondents (34%), and the frequency of age in the late elderly category was more dominant, namely 13 people (41%). Based on the length of time they have suffered from diabetes mellitus, with >10 years, there are 20 people (63%). The frequency of blood glucose increasing based on Point of Care Testing (POCT) examination was 23 people (72%). The urine culture results of diabetes mellitus patients showed that the average urine bacteria number was 6,3.103 CFU/mL with the interpretation of possible urinary tract infection (UTI), namely 11 people (34%), and 6,0.101 CFU/mL with the interpretation of no UTI, being 21 people (66%). Conclusion: Based on the research results, it was concluded that 3 respondents (9%) had positive leukocyte values despite the possibility of an UTI, and 8 respondents (25%) had negative leukocyte values despite the possibility of an UTI. There were 11 respondents (34%) with increased blood glucose accompanied by the possibility of UTI, and 3 respondents (9%) with increased blood glucose accompanied by positive leukocyte values.
Comparison of Tuberculosis Examination Using Ziehl-Neelsen Method and Molecular Rapid Test Anwar, Aan Yulianingsih; Handayani, Marini Tri Putri; Nurdin, Erpi; Lewa, Irma Berliana,HI; Artati, Artati
Jurnal Kesehatan Manarang Vol 9 No 3 (2023): December 2023
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v9i3.848

Abstract

Tuberculosis is an infectious disease of the respiratory tract caused by infection with the bacteria Mycobacterium tuberculosis. Fast and accurate diagnostic methods are very important for diagnosing and controlling pulmonary TB disease in Indonesia. TB examination using the Zn method is very commonly used even though it has lower sensitivity than TCM. The aim of this study was to compare the results of TB examination using BTA staining with the Molecular Rapid Test. This type of research uses analytical observational methods with a cross-sectional approach. The sample was 40 respondents taken using purposive sampling technique. The results of tuberculosis examination using the Ziehl-Neelsen (ZN) BTA staining method and the Moluker rapid test tool (TCM) method showed that the microscopic results of the Ziehl-Neelsen method of BTA smear staining showed negative results in 31 people (75%), 1 respondent (2 .5%) with scanty results and 9 respondents (22.5%) with 1+ results. The results of the TB BTA examination using the TCM method resulted in MTB Not Detected with a total of 30 people (75%), MTB Detected Medium obtained a result of 6 people (15%), MTB Detected Low obtained a result with a total of 3 people (7.5%), MTB Detected Very Low results were obtained with the number of 1 person (2.5%) and in the SPSS results using the t-test the results obtained were p value <0.05 (0.012 <0.05) which means there is a comparison of the BTA staining tuberculosis examination using the Ziehl-Ziehl method. neelsen and Molecular Rapid Tests. Based on the research results, it was found that there was a significant difference between the Ziehl-Neelsen BTA staining tuberculosis examination method and the Molecular Rapid Test Tool with a p value <0.05 (0.012 <0.05)
Deteksi Gen Resistensi Karbapenemase Dan Metallo-Β-Lactamase Pada Acinetobacter Baumannii Zuchrullah, Mukhtasyam; Nurdin, Erpi
Jurnal Media Analis Kesehatan Vol 15 No 1 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i1.631

Abstract

Peningkatan resistensi terhadap antibiotik golongan karbapenem merupakan salah satu fenomena yang harus diwaspadai. Acinetobacter baumannii merupakan salah satu bakteri penyebab infeksi berat yang didapatkan di rumah sakit. Penelitian ini bertujuan untuk mendeteksi gen intrinsik dan gen yang membawa resistensi obat golongan karbapenem terhadap A. baumannii. Penelitian ini dilaksanakan di Laboratorium HUM-RC RSPTN Universitas Hasanuddin Makassar. Desain penelitian ini adalah deskriptif eksperimental laboratorium dengan jumlah sampel 50 isolat yang didapatkan dengan cara total sampling yang dikumpulkan dari RSUP Wahidin Sudirohusodo Makassar. Data yang dianalisis adalah hasil karakteristik isolat, uji sensitivitas dengan menggunakan Vitek-2, uji genotipe gen intrinsik OXA-51 dan blaIMP-1 dengan menggunakan Polymerase Chain Reaction. Hasil penelitian menunjukkan bahwa dari total 50 isolat A. baumannii, ditemukan paling banyak pada sampel sputum sejumlah 30 isolat (60%). Dari total 50 sampel uji sensitivitas antibiotik, isolat A. baumanni paling banyak resisten terhadap meropenem 14 isolat (28%) kemudian imipenem 9 isolat (18%), dan Doripenem 9 isolat (18%). Gen intrinsik OXA-51 ditemukan pada semua isolat sampel sedangkan hanya satu isolat dari A. baumannii yang mampu memproduksi enzim metallo-β-Lactamase (MBL) dan membawa gen blaIMP-1 yaitu pada sampel urine yang seluruhnya resisten terhadap ketiga golongan obat karbapenem. Kata Kunci : Acinetobacter baumannii, Karbapenem, MBL, Polymerase Chain Reaction
PEMANFAATAN MEDIA ALTERNATIF DALAM ISOLASI BAKTERI PADA URINE PENDERITA INFEKSI SALURAN KEMIH DI WILAYAH DAERAH BINAAN KELURAHAN DUFA-DUFA KOTA TERNATE Erpi Nurdin; Jakaria, Febrianti; Hasanuddin, Riskawati
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1775

Abstract

Bacteriological examination, especially bacterial culture, is the gold standard for infectious diseases. The very limited availability of culture media encourages the use of alternative media, especially to see the presence of bacteria in the urine of patients with Urinary Tract Infections (UTIs). The price of semi-synthetic culture media is quite expensive and it is difficult for producers to obtain it in the North Maluku region, especially in Ternate City. The use of this alternative media is expected to provide direct benefits to detect the presence of bacteria through the urine of patients with UTIs and improve the knowledge and skills of ATLM cadres regarding the procedure for using alternative media. The use of tuna as one of the alternative media ingredients has not been optimal for the purposes of microbiology laboratory diagnostics. Carried out in the form of socialization which is implemented in the form of lectures, presentations of materials by the community service team with material on the use of alternative media for examining bacteria that cause UTIs. This activity also involves the activeness of participants by working on assessment worksheets and ending with a post-test activity. In the form of knowledge and application of the Use of alternative culture media based on tuna in examining urinary tract infections in the Dufa-Dufa Village community. The test results showed an increase in the percentage of understanding of community service participants, where at the beginning of the activity there were 54% who did not understand UTI and microbiology laboratory examinations, after receiving education there were no more participants who did not understand. In the choice of understanding enough at the beginning of the test 42% and post test 0%. From the criteria understood at the beginning with only 4%, increased to 48%. In the criteria very understood from 0% increased to 52%. The results of the UTI examination through urine culture with alternative media were that there was UTI in 32 people (64%) and the possibility of UTI in 18 people (36%). Increased understanding of the importance of conducting urine culture examinations related to UTI up to 100%, where 48% understood and 52% understood very much. There were 64% of participants with UTI and the possibility of UTI 36%.
BACTERIAL CULTURE PROFILE ON THROAT SWAP OF PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION AT KALUMPANG HEALTH CENTER TERNATE CITY Erpi Nurdin; Mukhtasyam Zuchrullah; Fifi B Umagapi
Jurnal Inovasi Pendidikan dan Sains Vol 4 No 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v4i3.1899

Abstract

Abstract: Respiratory tract infection is a common disease in society, and is one of the highest causes of death in children under 5 years of age (22.30%). Acute respiratory tract infections are divided into lower respiratory tract infections and upper respiratory tract infections. This infectious disease can be carried out by bacteriological examination using selective media to see the bacterial species. The aim of the research was to determine the bacterial culture profile in throat swabs of patients with acute respiratory infections at the Kalumpang Community Health Center, Ternate City using selective media, biochemical tests and gram staining. Research conducted using descriptive methods. Based on data on the characteristics of the Acute Respiratory Infection (ARI) patients studied, there were 17 men (57%) and 13 women (43%), while the age group with the most sufferers was in the 1-10 year age range, namely 9 people (30%) and then in the age range >50 years, namely 8 people (27%). Meanwhile, the percentage of culture profile findings from the bacterial species Stapylococcus aureus was 50%, Streptococcus pygones 33%, Pseudomonas aeruginosa 7%, Corynebacterium diphtheriae 10%. Found 98% gram positive bacteria and 2% gram negative bacteria. From the research results, it can be concluded that the most common bacterial culture profile in the throat swabs of upper respiratory tract infection patients is gram-positive bacteria consisting of the species Stapylococcus aureus, Streptococcus pygones, and Corynebacterium diphtheriae. The gram negative bacterial profile obtained was Pseudomonas aeruginosa. Keywords : Acute Respiratory Infections, throat swab, culture, bacteria