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Hubungan Cemaran Bakteri Salmonella Typhi pada Feses Anak Terhadap Personal Higiene di Kelurahan Kampung Makassar Timur Kota Ternate erpi nurdin
Jurnal Kesehatan Vol 11 No 1 (2018): Jurnal Kesehatan Published By Poltekkes Ternate, Juni 2018
Publisher : UPPM Poltekkes Kemenkes Ternate

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.428 KB) | DOI: 10.32763/juke.v11i1.122

Abstract

Cemaran bakteri Salmonella typhi pada feses sangat erat kaitannya dengan hygiene pribadi. Penularan penyakit ini hamper selalu terjadi melalui makanan dan minuman yang terkontaminasi, yang menyebabkan penyakit abdominalis. Infeksi bakteri Salmonella typhi diperkirakan angka insidensi di seluruh dunia terdapat sekitar 17 juta per tahundengan 600.000 orang meninggal. WHO memperkirakan 70% kematianterjadi di Asia.Kampung Makassar merupakan kelurahan dengan sanitasi yang belum memadai. Penelitian ini bersifat komparatif dan bertujuan untuk mengetahui hubungan cemaran bakteri Salmonella typhi terhadap personal higiene anak di Kelurahan Kampung Makassar Kota Ternate.Teknik pengambilan sampel adalah secara non random dengan desain purposive sampling sebesar 40 orang. Sampel tersebut kemudian dibuat perlakuan untuk diidentifikasi Salmonella typhi dengan metode kultur feses. Dari hasil penelitian di peroleh hasil 17 sampel positif terdeteksi positif sebagai Salmonella typhi, dengan tingkat hygiene 3 buruk, 30 cukup baik , dan 7 baik. Berdasarkan hasil pengolahan data diperoleh p value 0,037<0,05. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat hubungan yang bermakna antara cemaran Salmonella typhi pada feses anak terhadap personal hygiene.
Spill Kit Infeksius berbahan Virgin Coconut Oil 71% Efektif sebagai Desinfektan Mikroba Urin penderita Infeksi Saluran Kemih: Penelitian Laboratorium Hi Lewa, Irma B; Nurdin, Erpi
Health Information : Jurnal Penelitian Vol 15 No 3 (2023): September-Desember
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36990/hijp.v15i3.1154

Abstract

Virgin coconut oil atau VCO merupakan salah satu hasil olahan buah kelapa (Cocos nucifera L.) yang mengandung asam lemak sehingga berpotensi memiliki antimikroba. Spill kit merupakan peralatan yang digunakan untuk membersihkan material yang berbahaya atau infeksius yang berbentuk cair. Namun, penggunaan cairan bahan kimia dari komponen spill kit secara terus menerus akan berdampak buruk pada lingkungan. Penelitian ini bertujuan untuk mengetahui efektivitas VCO sebagai bahan spill kit desinfektan. Jenis penelitian yang dilakukan adalan deskriptif dengan pendekatan eksperimental. Sampel yang digunakan adalah urin penderita infeksi saluran kemih pada Puskesmas. Perlakuan urin dengan pengkulturan pada media agar dengan jenis kultur urin, kultur kemudian diberikan perlakuan vco dan klorin. Berdasarkan pengamatan kultur pada spesimen yang dilanjutkan dengan pewarnaan bakteri diperoleh gram positif 40% dan terdapat gram negatif 60%. Rerata jumlah koloni pada sampel urin yaitu 42 koloni, VCO 12 koloni, dan klorin tidak teramati pertumbuhan koloni. Rerata angka kuman pada urin sebelum perlakuan yaitu 1,4.104 CFU/ml, setelah perlakuan penambahan clorin pada urin yaitu 3,3.101 CFU/ml dan setelah penambahan VCO pada urin yaitu 4,1.103 CFU/ml. Terdapat penurunan angka kuman 1,4.104 CFU/ml (100%) pada penambahan Klorin dan penurunan 9,9.103 CFU/ml (71%) pada penambahan VCO. Efektivitas VCO sebagai pengganti klorin untuk desinfektan pada pertumbuhan mikroba urin yakni 71% sedangkan Klorin 100%. perlu dilakukan penambahan waktu suspense VCO untuk mendapatkan hasil sebagai disinfektan yang lebih maksimal.
Antibiotic Sensitivity Testing of Bacteria Isolated from Patients Suspected of Urinary Tract Infection: Uji Sensitivitas Antibiotik terhadap Bakteri yang Diisolasi dari Pasien Diduga Infeksi Saluran Kemih Erpi Nurdin; Suanbani, Dawika; Mukhtasyam Zuchrullah
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.1883

Abstract

Urinary tract infections (UTIs) is a condition wherein inflammation occurs, caused by the proliferation of microorganisms in the human urinary tract. Antibiotics are the main choice in treating UTIs, the aim of giving antibiotics is to treat acute infections and prevent urosepsis. However, deviant use of antibiotics will reduce the sensitivity of antibiotics to bacteria. The aim of this study was to determine the sensitivity of bacteria to antibiotics from urine specimens of patients with suspected urinary tract infections (UTIs). The examination begins with the determination of the bacterial species and continues with the sensitivity test of the Kirby-Bauer diffusion method, looking at the barrier zone of each antibiotic. The observation results showed that there were 6 types of bacteria found from the urine culture results of patients with suspected urinary tract infections, with the most types of bacteria being gram positive S. aureus in 13 cases (40%), gram negative E. coli in 6 cases (18%), K. oxytoca and E. aerogenes were 5 cases (15%). According to the results of the antibiotic sensitivity test, each one shows the percentage of sensitive antibiotics. Ciprofloxacin is 100% against E. coli, K. oxytoca, P. mirabilis, 60% against E. aerogenes and 46% against S. aureus. Gentamicin is 100% against E. coli, K. oxytoca, P. mirabilis. Ampicillin is 83% against E. coli, 60% against S. epidermidis. Amoxicillin is 66% against E. coli. In conclusion, based on test results, Ciprofloxacin has better sensitivity, followed by gentamicin, ampicillin and amoxicillin. Keywords: antibiotics, sensitivity testing, urinary tract infection
Observation of Candida spp. in the urine of Female Teens : Observation of Candida spp. in the urine of Female Teens Nurdin, Erpi; Mukhtasyam Zuchrullah; Nofita Sari Djurumudi
Bionature Vol. 25 No. 2 (2024): Oktober-Desember 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i2.4529

Abstract

Candida albicans is an opportunistic pathogenic fungus that most often causes urinary tract infections. The presence of C. albicans and NCA species in urine is known as candiduria, which can cause urinary tract infections. The presence of C. albicans in urine is caused because the female urethra is shorter than that of men. Research purposes to determine the frequency distribution and presentation of the presence of Candida spp. in the urine of female adolescents at the Hidayatullah Islamic Boarding School in Ternate City. Method this study uses a descriptive research type. This study describes the presence or absence of Candida spp. in the urine of female adolescents at the Hidayatullah Islamic Boarding School in Ternate City. Research results show obtained 40 urine samples of female adolescents examined, it was found that 14 samples (35%) were positive for C. albicans and 26 samples (65%) were negative for C. albicans. Results of this study concluded that 35% of the urine of female adolescents at the Hidayatullah Islamic Boarding School in Ternate City was infected with C. albicans. Keywords: Candida albicans, candiduria, urinary tract infection
Profil Kecacingan dan Kadar Hemoglobin Pada Ibu Hamil Dalam Upaya Pencegahan Stunting di Puskesmas Wilayah Kota Ternate Puasa, Rony; Yulianingsih, Aan; Nurdin, Erpi; Rafika, Rafika
Jurnal Media Analis Kesehatan Vol 16 No 1 (2025): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v16i1.1127

Abstract

Masalah kesehatan ibu hamil merupakan isu penting dalam upaya meningkatkan kesejahteraan ibu dan bayi, serta mencegah berbagai komplikasi yang dapat mempengaruhi pertumbuhan dan perkembangan anak. Di Indonesia, stunting, yang ditandai dengan tinggi badan anak yang kurang dari standar usianya, masih menjadi tantangan utama dalam kesehatan masyarakat. Kecacingan pada ibu hamil dapat menyebabkan anemia karena kehilangan zat besi, dan anemia yang tidak ditangani dapat meningkatkan risiko bayi lahir dengan berat badan rendah atau stunting akibat kekurangan asupan nutrisi selama kehamilan. Infeksi cacing, terutama oleh cacing kremi, cacing gelang, dan cacing tambang, dapat mengganggu penyerapan nutrisi dan menyebabkan defisiensi zat besi, yang berdampak pada kadar hemoglobin. Tujuan penelitian ini untuk menggambarkan profil kecacaingan dan kadar Hb pada ibu hamil. Penelitian ini dilaksanakan di Laboratorium Poltekkes Kemenkes Ternate. Desain penelitian ini adalah deskriptif  dengan jumlah sampel sebanyak 98 responden yang diambil secara random sampling dari 8 Puskesmas kemudian diperiksa kadar Hb pada darah dan telur cacing pada feses responden. Hasil penelitian pada 98 responden ibu hamil tidak menunjukkan adanya telur cacing pada feses dan terdapat 46 responden yang mengalami anemia dengan anemia ringan sebanyak 30 responden (65,2%) dan anemia sedang sebanyak 16 responden (16,4%).
Bacterial Resistance Test to Antibiotics Through Throat Swab Specimens of Children with Acute Respiratory Tract Infections (ARIs) Nurdin, Erpi; Zuchrullah, Mukhtasyam; Halik, Sofyanti Abdul
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.479

Abstract

Acute Respiratory Infections (ARIs) are contagious diseases that affect the respiratory tract and remain a leading cause of morbidity and mortality, particularly among children. The use of antibiotics without proper sensitivity testing can lead to bacterial resistance; therefore, research is needed to determine the effectiveness of commonly prescribed antibiotics. ARIs is one of the primary causes of morbidity and mortality, especially in children in developing countries. The irrational use of antibiotics or the absence of adequate sensitivity testing is one of the main factors contributing to the rise in bacterial resistance, which may reduce the effectiveness of treatment and increase the public health burden. Objective to determine the sensitivity levels of several types of antibiotics against bacteria that cause ARIs, obtained from throat swab specimens of children with ARIs in several community health centers (Puskesmas) under the Ternate City Health Office. This study is a descriptive research using a laboratory approach, applying the Kirby-Bauer method for antibiotic sensitivity testing against bacteria using Mueller Hinton Agar (MHA) media. Bacterial identification showed a predominance of Staphylococcus epidermidis (45%), followed by Streptococcus pneumoniae (35%), and Streptococcus pyogenes (20%). Sensitivity testing revealed that Streptococcus pneumoniae was most sensitive to gentamicin (100%), while Staphylococcus epidermidis showed high sensitivity to ciprofloxacin (100%). On the other hand, cefixime exhibited the highest level of resistance. Sensitivity testing is crucial before administering antibiotics to prevent resistance and ensure appropriate treatment for children with ARIs.
PEMANFAATAN MEDIA ALTERNATIF DALAM ISOLASI BAKTERI PADA URINE PENDERITA DIABETES MELLITUS DI WILAYAH DAERAH BINAAN KELURAHAN DUFA-DUFA KOTA TERNATE Nurdin, Erpi; Mukhtasyam Zuchrullah; Lewa, Irma B. Hi
Jurnal Abdi Insani Vol 12 No 9 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i9.2811

Abstract

Background: Bacteriological examination, particularly bacterial culture, is a standard method for diagnosing infectious diseases, including in patients with Diabetes Mellitus (DM). However, the limited availability and high cost of semi-synthetic culture media pose significant challenges, especially in regions such as North Maluku, including Ternate City. These constraints have encouraged the exploration of alternative culture media, one of which utilizes tuna-based ingredients. This alternative is expected to be applicable for urine culture tests in DM patients and to enhance the technical competencies of laboratory personnel. Nevertheless, the utilization of tuna as a component of alternative media for microbiological diagnostic purposes remains suboptimal. Methods: The activity was conducted in the form of community outreach in Dufa-Dufa Subdistrict through lectures, presentations, discussions, and evaluations using pre-tests and post-tests. The materials delivered focused on diabetes mellitus and the use of alternative media in microbiological examinations. Results: The results indicated a significant improvement in participants understanding. Initially, 51% of participants had no knowledge of DM and laboratory testing, which decreased to 0% after the educational session. The percentage of participants with moderate understanding declined from 44% to 0%, while those with good understanding increased from 5% to 49%, and those with excellent understanding rose from 0% to 51%. Additionally, the results of urine culture tests on patients with diabetes mellitus using tuna-based media showed that 90% of participants showed bacterial growth and 10% showed no bacterial growth. Conclusion: This initiative demonstrates that locally sourced alternative media hold promise as a feasible solution for laboratory diagnostics. Furthermore, the activity effectively raised community awareness regarding the importance of early detection of potential infections in patients with diabetes mellitus. Keywords: Diabetes mellitus, Urine culture, Alternative media, Laboratory diagnostics
Pewarnaan Alternatif dengan Menggunakan Filtrat Kulit Kenari pada Uji Mikroskopik Jamur Candida Albicans dan Aspergillus Niger Erpi Nurdin
Bionature Vol. 23 No. 1 (2022): April 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kenari merupakan tanaman asli Indonesia yang banyak tumbuh didaerah Indonesia bagian timur, seperti Sulawesi Utara, Maluku dan pulau Seram.Kulit kenari mengandung senyawa fenolik yang merupakan antioksida alami.Senyawa fenolik dipakai sebagai zat pengawet karena fenol dapat menangkapradikal bebas. Warna yang di kandung oleh kulit kenari memungkinkan sebagaialternatif pada pewarnaan uji mikroskopik jamur. Penelitian ini bertujuan Untukmelihat jamur pada pewarnaan alternatif kulit kenari dengan menggunakan ujimikroskopik. Metode yang digunakan dalam penelitian ini yaitu deskriptifdengan pendekatan eksperimen. Hasil penelitian diidentifikasi sebanyak 3sampel (100%) Candida albicans dan 3 sampel (100%) Aspergillus niger padafiltrat dengan perlakuan pemanasan, begitu pula 3 sampel (100%) Candidaalbicans dan 3 sampel (100%) Aspergillus niger pada filtrat dengan perlakukantanpa pemanasan. Pengamatan mikroskopik jamur Candida albicans danAspergillus niger, tampak lebih jelas dan terwarnai pada penggunaan filtratpewarnaan aternatif filtrat kulit kenari dengan proses pemasan. Kesimpulan :Pewarnaan alternatif dengan filtrat kulit kenari dapat di gunakan dalammewarnai jamur Candida albicans dan Aspergillus niger. Filtrat denganpemanasan lebih efektif pada uji mikroskopik jamur.
Studi Tuberkulosis Kontak Serumah pada Masyarakat Pesisir di Wilayah Kerja Puskesmas Siko Kota Ternate Erpi Nurdin; Irma B. Hi. Lewa; Mukhtasyam Zuchrullah
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pulmonary tuberculosis is still the main cause of death in the world, accounting for 2 million people die every year. WHO states that around 1.9 billion people, one third of the world's population, have been infected with tuberculosis germs, every second one person is infected with tuberculosis in the world. Pulmonary tuberculosis is transmitted through droplet spread with the source of infection being patients who are coughing with household contacts as a high risk group for transmission. To find out the results of the examination and see the percentage of positive or negative acid-fast bacilli (AFB) in a sample of clients who have a history of household contact with patients with pulmonary tuberculosis. This study uses a with correlation approach research method with an observational approach. The sampling technique is purposive sampling, namely sampling based on the considerations of the researcher. The examination method used was the Zielh Nelseen method of 30 sputum samples examined. From 30 respondents, 4 respondents were found positive for microscopic acid-fast bacilli (AFB). 2 positive family members, have the characteristics of dense housing (not eligible), humidity and ventilation do not meet the requirements, but the type of floor still meets the requirements. Based on the research that has been carried out, it is concluded that 4 (13%) respondents were identified as positive for scanty with the presence of tuberculosis in 3 places of tuberculosis in household contacts, with factors that influence the occurrence of tuberculosis in household contacts. There is no significant relationship between the results of AFB examination and gender (p value = 0.407> 0.05). There is no significant relationship between the results of AFB examination and age (p value = 0.272> 0.05).
Analisis Leukosit Urine Dipstik dan Kultur Bakteri Pada Pasien Diabetes Mellitus di Kota Ternate Nurdin, Erpi; Zuchrullah, Mukhtasyam; Nasarudin, Sandi
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 8 No 1 (2024): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v8i1.735

Abstract

Background: Diabetes mellitus sufferers with poor diabetes control will generally develop urinary tract infections. Methods: This is a descriptive study, namely to obtain a dipstick urine leukocyte picture and bacterial culture in diabetes mellitus patients in Ternate City. Results: The research results showed that there were 21 female respondents (66%) and 11 male respondents (34%), and the frequency of age in the late elderly category was more dominant, namely 13 people (41%). Based on the length of time they have suffered from diabetes mellitus, with >10 years, there are 20 people (63%). The frequency of blood glucose increasing based on Point of Care Testing (POCT) examination was 23 people (72%). The urine culture results of diabetes mellitus patients showed that the average urine bacteria number was 6,3.103 CFU/mL with the interpretation of possible urinary tract infection (UTI), namely 11 people (34%), and 6,0.101 CFU/mL with the interpretation of no UTI, being 21 people (66%). Conclusion: Based on the research results, it was concluded that 3 respondents (9%) had positive leukocyte values despite the possibility of an UTI, and 8 respondents (25%) had negative leukocyte values despite the possibility of an UTI. There were 11 respondents (34%) with increased blood glucose accompanied by the possibility of UTI, and 3 respondents (9%) with increased blood glucose accompanied by positive leukocyte values.