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All Journal AGRIKAN Jurnal Ilmiah Agribisnis dan Perikanan Biosaintifika: Journal of Biology & Biology Education Humanika Afiasi : Jurnal Kesehatan Masyarakat Jurnal Biogenerasi Pharmaqueous BIOGENIC: Jurnal Ilmiah Biologi
Slamet Ifandi, Slamet
Faculty of Science and Mathematics, Universitas Diponegoro, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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BIOPROSPEKSI POTENSI AKTINOBAKTERI HALOFILIK SEBAGAI ANTIBAKTERI PATOGEN PADA MANUSIA DARI TAMBAK GARAM TALISE SULAWESI TENGAH Nurfadilah; Salsabila, Rani; Saskirana; Ifandi, Slamet; Inayah, Nur; Arumdani, Salsabila; Lindhemutianingrum Siradje, Andi
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.7040

Abstract

This study aims to explore the potential of halophilic actinobacteria from Talise salt ponds, Central Sulawesi, as candidates for producing antibacterial compounds. The study was conducted through isolation, macroscopic and microscopic characterization, biochemical tests, and testing of antibacterial activity against S. aureus and E. coli using the disc diffusion method. Data were analyzed descriptively based on observations and inhibition zone measurements. The results showed the acquisition of five halophilic actinobacteria isolates, namely TL1, TL2, TL3, TL4, and TL5, which have characteristics similar to the genus Streptomyces. Biochemical tests indicated unique metabolic capabilities, including the production of the catalase enzyme and sugar utilization in the TSIA test. All isolates showed antibacterial activity, with isolate TL5 providing the largest inhibition zone, namely 16.5 mm against E. coli and 6.5 mm against S. aureus. These findings indicate that halophilic actinobacteria from Talise salt ponds have the potential to be a source of new antibiotics relevant for drug development in addressing antibiotic resistance
Etnofarmakognosi Tumbuhan Obat Masyarakat Suku Kaili di Dusun Tompu Sulawesi Tengah Ifandi, Slamet; Sulistiyaningsih, Yuyun
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 4 No. 2 (2022): Volume 4, Nomor 2, November 2022
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v4i2.304

Abstract

Dalam kehidupan sehari-harinya msyarakat Suku Kaili di Tompu masih mengandalkan pengelolaan sumberdaya alam hayati secara tradisional. Salah satunya praktek pemanfaatan jenis-jenis tumbuhan obat untuk pemeliharaan kesehatan telah ada sejak turun-menurun sebagai bagian dari sifat dan kearifan lokal mereka miliki. Metode penelitian ini adalah deskriptif menggunakan pendekatan survey eksploratif. Teknik pengumpulan data menggunakan wawancara secara mendalam, dan terstruktur dengan jumlah responden sebanyak 30 orang. Hasil penelitian diperoleh 15 jenis tumbuhan obat. 6 jenis diantaranya paling banyak digunakan untuk mengobati penyakit ringan seperti sakit perut. Bagian tumbuhan yang paling banyak digunakan yaitu daun, sedangkan cara pengolahan dan pemakaian tumbuhan obat paling banyak dengan cara dalam bentuk ramuan dan diminum. Hasil skrining uji kandungan fitokimia terhadap 4 jenis tumbuhan obat yang terpilih secara representatif didapatkan hasil positif mengandung seyawa alkaloid, flavanoid, saponin, tanin dan polifenol.
ISOLASI DAN KARAKTERISASI BAKTERI LAUT DARI OBJEK WISATA PUSAT LAUT DESA TOWALE KABUPATEN DONGGALA SULAWESI TENGAH Nugraini, Shinta; Permatasari, Ghita; Mutasim, Kholid; Humairah; Ifandi, Slamet; Lastri Gintoe, Hermin
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4389

Abstract

The Sea Center is a tourist attraction on the beach in the form of a natural well located in Towale village. Because it is unique and is widely used by the community for bathing, this tourist attraction needs to be researched to determine its potential, one of which is through the exploration of marine bacterial species, plus the research conducted is still very lacking or even non-existent. The purpose of this study was to isolate and characterize marine bacteria. The results showed that there were 6 isolates of marine bacteria obtained, namely isolates PS1, PS2, PS3, PS4, PS5, PS6. Macroscopic characterization observations obtained colony shape, elevation shape and shape of the most dominant edges are irregularly shaped, convex and flat. While microscopic observations obtained the nature of marine bacterial isolates, namely 4 isolates are Gram positive coccus-shaped and 2 isolates are Gram negative bacillus-shaped.
Co-Authors Alfan, Aris Alfan, Aris Alwi, Muh Arumdani, Salsabila Febriana Iffaf, Astri Fitri Nirwana Hasibuan, Fitri Nirwana Humairah Jumari Jumari, Jumari Lastri Gintoe, Hermin Lindhemutianingrum Siradje, Andi Mutasim, Kholid Nugraini, Shinta Nur Inayah Nurfadilah Permatasari, Ghita Rahma, Yustika Aulia Resmi, Fitroh Salsabila, Rani Saskirana Sri Widodo Agung Suedy Sulistiyaningsih, Yuyun