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All Journal Jurnal Biologi Tropis Indonesian Journal of Pure and Applied Chemistry Acta Solum
Miladiarsi, Miladiarsi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology

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MOLECULAR DOCKING AND ADME STUDIES OF STIGMASTEROL COMPOUNDS AS ANTI-BREAST CANCER Amir, Nur Insani; Nurfadillah, Arafah; Miladiarsi, Miladiarsi; Irma, Ade; Wahdaniar, Wahdaniar
Indonesian Journal of Pure and Applied Chemistry Vol 8, No 1 (2025)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v8i1.83224

Abstract

Molecular docking studies of stigmasterol (test ligand) and estradiol (control ligand) against the ER-α receptor were performed using PyRx software. This study aims to determine the interaction between stigmasterol ligands and ER-α receptors by the molecular docking method using PyRx. The study results show that stigmasterol and estradiol have the same binding energy value of -7.3 kcal/mol. The stigmasterol compound could therefore be used as a candidate for breast cancer drugs.
Water Content, Physical Quality of Seeds and Post-Harvest Fungal Attacks on Peanuts Miladiarsi, Miladiarsi; Tsaniyah, Barokati; Putri, Riska Awalia; Saputra, Hiksa Maulana; Sianturi, Yosua Pangihutan Pardamean Alextio
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9754

Abstract

The decline in peanut seed quality can result from improper post-harvest handling, including drying, pod peeling, and storage, which can damage the seeds and reduce their quality. Damaged seeds and high water content facilitate fungal growth on peanuts. Maintaining and ensuring peanut quality during market storage is very important. This study aims to examine the relationship between air content, the percentage of damaged seeds, and the percentage of seeds infected by post-harvest fungal species in peanut seeds. The experimental method involved measuring air content with a DELMHORST Model G-7 Moisture Meter. The physical quality of seeds was assessed based on the percentages of intact, wrinkled, and damaged seeds. To determine the percentage of seeds infected with fungi, the direct planting method was used, employing Dichloran 18% Glycerol Agar (DG18+) media. The results indicated that each sample’s air content was within the normal range of 8-9%, but the physical quality of the seeds was 59.7%, associated with fungal attacks by species including Aspergillus flavus, A. niger, A. tamari, Fusarium semitechtum, Mucor sp., Eurotium repens, and E. chevalieri. These findings suggest that air content and seed damage influence fungal diversity, providing valuable insights for maintaining peanut seed quality during the post-harvest process until they reach the market.
Exploration of Bacteria in Red Chili Plant Soil with Potential as Plant Growth Promoting Rhizobacteria (PGPR) Miladiarsi, Miladiarsi; Rizal, Muhammad; Wijaya, Padil
Acta Solum Vol 3 No 3 (2025): Juli 2025
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v3i3.3419

Abstract

Soil fertility depends on the availability of sufficient nutrients for plant absorption. Chemical fertilizers can be used to provide sufficient N and P, but this affects soil health. The presence of rhizobacteria act as biofertilizers by increasing nutrients and altering soil formation, especially in the phosphate and nitrogen cycles. Phosphate-solubilizing and nitrogen-fixing bacteria play a role in increasing soil fertility and improving unstable soil properties. This study aims to identify rhizobacteria that have the potential as PGPR to increase N and P levels in the soil. Soil samples were collected randomly at a depth of 10 cm around chili plant roots. Bacteria were isolated using serial dilution and cultured on Pikovskaya medium. A total of 27 isolates produced clear zones with the highest solubility index and were inoculated on Nitrogen-Free Bromthymol Blue Agar (NFB) to evaluate N fixation through a color change from green to blue, as well as hypersensitivity tests on tobacco plants. Isolates showing the highest P solubility, highest N fixation ability, and no necrosis in tobacco were inoculated into sterile soil to analyze compound changes. Soil N content was measured using the Kjeldahl method, while phosphate levels were analyzed using the Bray I and HCl methods. The results of the study showed that the addition of KE2.15 isolate to the soil after 4 weeks of application increased the total organic N and available P content, making it a potential biofertilizer.
Co-Authors Ade Irma Amir, Nur Insani Hiksa Maulana Saputra MUHAMMAD RIZAL Nurfadillah, Arafah Putri, Riska Awalia Sianturi, Yosua Pangihutan Pardamean Alextio Tsaniyah, Barokati W, Wahdaniar Wijaya, Padil