Sri Handayani
Institut Kesehatan Helvetia

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Evaluasi dan Potensi Daya Hambat Gel Antiseptik Tangan Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) terhadap Bakteri Patogen Pseudomonas aeruginosa dan Staphylococcus epidermidis Ruth Mayana Rumanti; Sri Handayani; Tetty Noverita Khairani; Ihsanul Hafiz
Journal of Pharmascience Vol. 10 No. 1 (2023): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v10i1.13490

Abstract

Penggunaan gel antiseptik tangan merupakan upaya untuk menghindari kontaminasi kulit dari bakteri patogen. Daun kemangi diketahui memiliki kandunganflavonoid, alkaloid, tanin, dan linalool yang efektif sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kestabilan dan daya hambat antibakteri gel antiseptik ekstrak etanol daun kemangi (Ocimum sanctum L.) terhadap bakteri patogen di udara yaitu Pseudomonas aeruginosa dan Staphylococcus epidermidis. Simplisia daun kemangi sebelumnya dikarakterisasi lalu diekstraksi secara perkolasi menggunakan etanol 96%. Ekstrak diformulasi menjadi sediaan gel antiseptik dan diuji kestabilannya dengan metode cycling test selama 6 siklus. Daya hambat antibakteri dengan metode difusi sumuran pada konsentrasi 1,5%; 3%; dan 4,5%. Data penelitian dianalisis secara statistik Uji One Way Anova. Hasil evaluasi kestabilan pada 6 siklus tidak ada perubahan yang signifikan pada pengujian organoleptis, viskositas, pH, daya sebar sementara pertumbuhan bakteri Pseudomonas aeruginosa dan Staphylococcus epidermidis dihambat pada konsentrasi 4,5% dengan diameter daerah hambat masing-masing sebesar 25,5 mm dan 26,6 mm termasuk kategori kuat. Kata Kunci: Daun Kemangi (Ocimum sanctum L.), Gel Antiseptik Tangan, Potensi Daya Hambat, Pseudomonas aeruginosa, Staphylococcus epidermidis The use of hand antiseptic gel is an effort to avoid skin contamination from pathogenic bacteria. Kemangi leaves are known contain flavonoids, alkaloids, tannins, and linalool which are effective as antibacterial. This study aims to determine the stability and antibacterial inhibition of the antiseptic gel of the ethanolic extract of Kemangi leaves (Ocimum sanctum L.) against pathogenic bacteria in the air, namely Pseudomonas aeruginosa and Staphylococcus epidermidis. The simplicia of kemangi leaves was previously characterized and then extracted by percolation using 96% ethanol. The extract was formulated into an antiseptic gel preparation and tested for stability by the cycling test method for 6 cycles. Inhibition of antibacterial by agar well diffusion method at concentrations of 1.5%, 3%, and 4.5%. The research data were statistically analyzed by the One Way Anova Test. The results of the stability evaluation at 6 cycles showed no significant changes in organoleptic testing, viscosity, pH, dispersion, while the growth of Pseudomonas aeruginosa and Staphylococcus epidermidis was inhibited at a concentration of 4.5% with a diameter of 25.5 mm and 26.6 mm respectively is included in the strong category.
The Formulation, Characterization, and Antibacterial Activity of Solid Soap Containing Guava Leaf Extract (Psidium guajava L.) and Precipitated Calcium Carbonate Sri Handayani; Hendri Faisal; Shaflina Izar; Togito Febrina Sitorus
Jurnal Kesmas Prima Indonesia Vol. 10 No. 2 (2026): July Edition
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jkpi.v10i2.8467

Abstract

Bath soap is defined as a sodium compound with fatty acids used as a body cleanser, which generates foam and does not cause skin irritation. Beyond cleansing the skin from external dirt, soap can also function to eliminate bacterial skin infections. Prior investigations have verified that the saponins, tannins, and flavonoids concentrated in guava leaves exhibit robust antibacterial activity. The objective of this study was to determine the physicochemical properties of a solid soap formula containing the ethanol extract of guava leaf and precipitated calcium carbonate, including texture, color, pH, moisture content, hardness, and other relevant properties. This study employed a laboratory experimental research method. The procedure involved the preparation of guava leaf (Psidium guajava L.) simplicia, followed by extraction with a 70% ethanol solvent via maceration. Subsequently, the solid soap formulation was created using the guava leaf extract and precipitated calcium carbonate, followed by evaluation and antibacterial activity testing against Staphylococcus aureus bacteria. The pH evaluation test yielded an average value of 9.09 for the blank formula, while the average pH values for the active formulas were 9.13 for FI, 9.30 for FII, 9.23 for FIII, and 9.12 for the positive control (comparator). Based on the antibacterial testing results, the solid soap containing guava leaf ethanol extract demonstrated strong antibacterial activity against Staphylococcus aureus. The inhibition zone diameters were recorded as 0 mm for F0 (blank), 11.6 mm for FI (10% concentration), 10.9 mm for FII (15% concentration), 11 mm for FIII (20% concentration), and 11.3 mm for K+ (comparator). The research indicates that the ethanol extract of guava leaves combined with precipitated calcium carbonate (PCC) can be successfully formulated into a stable solid soap that meets the physical quality standard requirements according to the Indonesian National Standard (SNI).