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All Journal Jurnal Pengmas Kestra (JPK) Medistra Medical Journal (MMJ)
Asvia Rahayu
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Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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SEMINAR IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Vincentia Ade Rizky; Sa'adah Siregar; Visensius Krisdianilo; Asvia Rahayu
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 1 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.53 KB) | DOI: 10.35451/jpk.v1i1.746

Abstract

The technique of identifying Escherichia coli O157: H7 bacteria in the feces of diarrhea sufferers using a molecular method, namely PCR has been proven to be more sensitive and shows fast results but is expensive when compared to conventional methods. Therefore, it can be recommended and used by health workers in early detection so that it will help make the diagnosis faster and determine treatment more effectively. The results of this seminar aim to make it easier for laboratory staff to identify the Escherichia coli O157: H7 bacteria in diarrhea sufferers.
Optimizing platelet count assessment: the potential of celery leaves (Apium graveolens linn) as a sustainable alternative to EDTA (Ethylenediaminetetraacetic Acid) Herlina H; ANGELIKA SINAGA; AGNES OCTAVIA DAMANIK; ASVIA RAHAYU; LASMARYNA SIRUMAPEA
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2092

Abstract

Seledri (Apium graveolens linn) mengandung flavonoid, fitosterol, vitamin K dan apigenin. Apigenin memiliki sifat sebagai vasodilator, yang memperlebar pembuluh darah dengan cara menghambat kontraksi yang disebabkan oleh pelepasan kalsium, mirip dengan mekanisme kerja antagonis kalsium. Antagonis kalsium bekerja dengan menurunkan tekanan darah melalui pemblokiran masuknya kalsium ke dalam aliran darah, mekanisme tersebut mirip dengan EDTA dan berpotensi sebagai antikoagulan yang menghambat agregasi trombosit. Penelitian ini bertujuan untuk mengevaluasi potensi daun seledri sebagai pengganti EDTA pada pemeriksaan jumlah trombosit. Metode yang digunakan adalah eksperimen, dengan Modifikasi pembuatan larutan seledri pada konsentrasi 30% dan 40%. Hasil pengujian menunjukkan bahwa larutan seledri 40% memberikan hasil hitung trombosit sebesar 196.000 sel/ul dan memiliki efek yang sama dengan antikoagulan EDTA sebagai kontrol. Analisis statistik menunjukkan tidak ada perbedaan signifikan antara seledri dan dengan konsentrasi 40% dengan EDTA, dan hal terbukti lebih efektif sebagai alternatif pengganti EDTA dalam pemeriksaan jumlah trombosit.
Analysis of colifrom bacteria of dishwasting water food stalls in Lubuk Pakam Rahmadani Sitepu; AHMAD ILHAM; ANGELIKA; ASVIA RAHAYU; RIMAYANI
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2112

Abstract

Food stalls play a role in helping fulfil daily needs by offering convenient and fast services, so people like to eat food at food stalls. If contaminated cutlery is used, those who use it may be infected with disease-causing microbes. Objective: The purpose of this study was to determine the presence of Coliform bacteria in water both before and after being used to clean food utensils in food stalls around the Medistra Institute of Health Lubuk Pakam. Methods: The method used is quantitative, as well as experimental to test water quality with applicable quality standards and describe the results of research based on literature review. Results: Of the 4 food stalls around the Medistra Institute of Health, it shows that there are 3 food stalls in the category of not meeting health requirements and 1 other food stall in the category of meeting health requirements based on the maximum number of total coliforms for piped and non-piped water 10/100 and 50/100 ml of water samples respectively in accordance with Permenkes No. 416/Per/Menkes/IX/1990. The quality of washing water (clean water) used in food stalls around the Medistra Institute of Health based on the results of laboratory tests of total Coliform, from four food stalls around the Medistra Institute of Health, 3 food stalls in the category of not meeting health requirements and 1 other food stall in the category of meeting the requirements according to Permenkes No. 416/Per/Menkes/IX/1990, concerning clean water quality for piped and non-piped water with numbers 10/100 ml and 50/100 ml of water samples. Keywords : Coliform bacteria, Escherichia coli, Dishwasting Water
Modification Of Star Fruit Wuluh (Averrhoa Bilimbi L.) As A Substitute For Turk Solution In The Manual Method Leukocyte Count. Selda Lemriani Manullang; Vincentia Ade Rizky; Asvia Rahayu
Medistra Medical Journal (MMJ) Vol 2 No 2 (2025): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i2.2374

Abstract

Background: This study counts the number of leukocytes using a manual method. The natural materials used are lime, lemon, and tamarind, which contain glacial acid and citric acid with a pH below 2.0. These acids can lyse blood cells, except for leukocytes (white blood cells). Objective: This study aims to analyze the effectiveness of varying concentrations of starfruit (Averrhoa bilimbi) on the number of leukocytes (white blood cells). Method: This research employs an experimental method. The number of leukocytes is counted from the juice of starfruit at concentrations of 5% and 10%. The objective is to find the most effective concentration compared to the standard Turk solution as a control. Conclusion: The data were analyzed using One-Way ANOVA. The average number of leukocytes in the control group was 6,500, in the 10% concentration was 6,450, and in the 5% concentration was 4,500. The results of statistical tests and hypotheses showed a significance value of 0.000, which is less than ? (alpha) (0.05), thus the null hypothesis is rejected and there is a significant difference. The 10% concentration proved to be the most effective in replacing glacial acetic acid in the Turk solution.
Differences in Tubex TF Results Based on Age and Gender Among Patients with Suspected Typhoid at Grandmed Hospital in Lubuk Pakam DIAN HASTARI NINGRUM; Asvia Rahayu; Melda Yulianda
Medistra Medical Journal (MMJ) Vol 4 No 1 (2026): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/z5whv531

Abstract

Typhoid fever remains an important enteric infection, particularly in areas where fecal-oral transmission persists. Tubex TF is a semi-quantitative serological assay used to detect IgM antibodies against the O9 antigen of *Salmonella Typhi*. This study aimed to analyze differences in Tubex TF results based on age and sex among patients with suspected typhoid fever at Grandmed Hospital, Lubuk Pakam. An observational analytic study with a cross-sectional design was conducted on 30 eligible patients from January to May 2026. Serum samples were examined using Tubex TF and classified as negative (score ≤2), weak positive (score 4), and strong positive (score 6–10). Data were analyzed descriptively and using the Pearson Chi-square test with a significance level of 0.05. Of the 30 samples, 2 (6.7%) were negative, 13 (43.3%) were weak positive, and 15 (50.0%) were strong positive. Among male patients, 6 had weak-positive and 9 had strong-positive results. Among female patients, 2 had negative, 7 had weak-positive, and 6 had strong-positive results. The adult group showed the highest number of strong-positive results, with 6 of 10 subjects. Statistical analysis showed no significant difference in Tubex TF results based on sex (p=0.262) or age group (p=0.336). In conclusion, Tubex TF results among patients with suspected typhoid fever at Grandmed Hospital were not significantly different according to age or sex. These findings suggest that demographic characteristics alone are insufficient to predict Tubex TF result categories in this study population. Therefore, interpretation should remain integrated with clinical findings, disease duration, and other relevant laboratory examinations.
Co-Authors AGNES OCTAVIA DAMANIK Ahmad Ilham Angelika ANGELIKA SINAGA DIAN HASTARI NINGRUM Herlina H Lasmaryna Sirumapea Melda Yulianda RIMAYANI Sa'adah Siregar Selda Lemriani Manullang Sitepu, Rahmadani Vincentia Ade Rizky Visensius Krisdianilo