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All Journal Indonesian Journal of Tropical and Infectious Disease Biomedika JURNAL ANALIS LABORATORIUM MEDIK
Dewajani Purnomosari, Dewajani
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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PEMBERIAN TEPUNG GANYONG (Canna edulis Kerr.) MENURUNKAN EKSPRESI Ki67 PADA JARINGAN USUS BESAR TIKUS YANG DIINDUKSI AOM/DSS Mahmudah, Nur; Purnomosari, Dewajani; Sumiwi, Yustina Andwi Ari
Biomedika Vol 10, No 2 (2018): Biomedika Agustus 2018
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v10i2.7017

Abstract

ABSTRAKKanker usus besar merupakan kanker yang memiliki insiden dan mortalitas tinggi. Karsinogenesis ditandai oleh proliferasi sel yang tidak terkendali, yang dapat dilihat dengan adanya peningkatan ekspresi Ki67. Kejadian kanker usus besar meningkat akhir-akhir ini yang dimungkinkan terjadi akibat pola makan masyarakat modern yang buruk. Masyarakat modern lebih menyukai makanan cepat saji, yang rendah serat dibandingkan makanan sehat yang kaya serat, seperti ganyong. Penelitian ini bertujuan untuk mengkaji efek pemberian ganyong terhadap ekspresi Ki67 pada jaringan usus besar tikus yang diinduksi AOM/DSS. Jenis penelitian ini merupakan eksperimental dengan post test only group design. Penelitian ini menggunakan tikus model kanker usus besar yang dibuat dengan induksi AOM/DSS. Perlakuan ganyong diberikan selama 16 minggu, diberikan mulai 2 minggu sebelum induksi AOM hingga sebelum terminasi. Ekspresi ki67 dilihat dengan metode imunohistokimia, kemudian dihitung menggunakan software Image J. Hasil penelitian menunjukkan bahwa pemberian ganyong tidak menurunkan ekspresi Ki67 pada tikus yang diinduksi AOM/DSS (p= 0,101). Kesimpulannya bahwa pemberian ganyong pada tikus yang diinduksi AOM/DSS tidak menghambat proliferasi sel.Kata Kunci: Ganyong, Ki67, Induksi Aom/Dss. ABSTRACTColon cancer is a cancer that has a high incidence and mortality. Carcinogenesis is characterized by  uncontrolled cell proliferation, which can be seen in the presence of increased Ki67 expression. The incidence of colon cancer has increased in recent years, which may have been due to the poor diet of modern society. Modern society prefers fast food, which is low in fiber compared to healthy foods rich in fiber, such as ganyong. This study aims to examine the effect of canna administration on Ki67 expression in AOM / DSS-induced rectum colon tissue. This research type is experimental with post test only group design. This study used a mouse model of colon cancer made with AOM / DSS induction. Canna administration was given for 16 weeks, given 2 weeks before AOM induction until termination. Ki67 expression seen with imunohistokimia method, then calculated using Image J software. The results showed that canna administration did not decrease of Ki67 expression in rats induced AOM / DSS (p = 0,101). In conclusion that canna administration to rats induced by AOM / DSS did not inhibit cell proliferation.Keywords: Canna, Ki67, AOM / DSS Induction.
Different COVID-19 mRNA-based Vaccine Platforms as The Booster Dose and Their Impact on Omicron: A Literature-Based Overview Mahdi, Bagus Aulia; Soegiarto, Gatot; Wulandari, Laksmi; purnomosari, dewajani
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 2 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i2.39597

Abstract

Globally, the vaccine has been determined as one of the principal policies to tackle the COVID-19 pandemic. However, some vaccinated individuals with two complete doses of inactivated experienced SARS-CoV2 infection, including the healthcare workers (HCWs). This threat led to the emergent need for a vaccine booster with different types of platforms aiming to enhance immunity from the Omicron variant. We conducted a literature study on the concept of heterologous compared to homologous vaccines in COVID-19 vaccination. We obtained 22 studies about COVID-19 booster vaccines. Referring to seven of them, we compared and distinguished between heterologous and homologous vaccines. We then reported the literature review according to PRISMA guideline. The study demonstrated qualitatively that heterologous vaccinations boosted antibody receptor binding domain, neutralizing antibody, and spike-specific Th1 type T cell responses and had an impact on omicron infection when compared to homologous vaccines. In conclusion, heterologous, mRNA based vaccine, predominantly induces cellular and humoral responses better than the homologous vaccine. This increased immune response is expected to provide profound immunity against the Omicron.
Impact of Hypertension and Cardiovascular Diseases to Immune Response in COVID-19 Vaccination: A Systematic Review Fahmita, Karin Dhia; Soegiarto, Gatot; Wulandari, Laksmi; Purnomosari, Dewajani
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 1 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i1.40266

Abstract

To determine impact of hypertension and cardiovascular diseases towards effectivity and safety of COVID-19 vaccination. Systematic review based on PRISMA statement was done. Searching was conducted in PubMed, ScienceDirect, Scopus, and ProQuest and resulting in 6 studies involving 4,053 participants which deemed on good quality according to Joanna Briggs Institute tools for critical appraisal. After thorough analysis, we found that two out of four studies assessing mRNA-based vaccine found out that hypertension lower antibody response significantly. Two out of two studies assessing inactivated virus vaccine shown that hypertensive patients tend to have lower antibody titers compared to control. One of studies mentioned above found that antibody titer was not different between populations with cardiovascular diseases and control.Hypertension lessened response to COVID-19 vaccination regardless of vaccine type used. However, lack of studies on cardiovascular disease suggested that more studies should be conducted, along with hypertension, in-order to make meta-analysis possible to provide better evidence.
PENGARUH MASA PENYIMPANAN SAMPEL WHOLE BLOOD DAN BUFFY COAT DARAH TIKUS TERHADAP KUANTITAS DAN INTEGRITAS RNA Winarti, Retno; Ramadhani Nurhidayat, Annisa; Purnomosari, Dewajani; Suhda, Saihas; Susilowati, Rina
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i2.6410

Abstract

Latar belakang: Whole blood dan buffy coat merupakan dua sumber sampel yang sering digunakan dalam penelitian molekuler. Namun, kualitas RNA yang dihasilkan seringkali kurang memuaskan. Faktor yang diperkirakan dapat mempengaruhi kualitas RNA adalah jenis sampel dan kondisi penyimpanan. Tujuan: untuk mengetahui pengaruh masa penyimpanan sampel whole blood dan buffy coat terhadap kuantitas dan integritas RNA, membandingkan kedua tipe sampel. Sampel darah dari tikus Sprague dawley umur 3 bulan diambil melalui sinus retro-orbitalis dan cardiac punture lalu dipisahkan sebagai whole blood dan buffy coat dan disimpan pada suhu −80°C selama 1 hari, 1 minggu, 1 bulan, dan 3 bulan. RNA diisolasi menggunakan kit Quick-RNA Miniprep Plus Zymo dan diperiksa konsentrasi serta kemurniannya dengan spektrofotometer (A260/A230 dan A260/A280). Integritas RNA diperiksa dengan elektroforesis agarose dan dibaca menggunakan Gel Doc. Metode: Data dianalisis menggunakan uji Two-way ANOVA. Analisis menunjukkan bahwa tipe sampel merupakan faktor utama yang mempengaruhi kuantitas dan integritas RNA. Hasil: Buffy coat menghasilkan konsentrasi RNA yang lebih tinggi dibanding whole blood (p < 0,0001). Faktor lama penyimpanan hingga 3 bulan pada suhu −80°C tidak mempengaruhi konsentrasi RNA pada kedua jenis sampel. Integritas RNA buffy coat lebih baik dibandingkan whole blood. Kesimpulan: Hal ini ditunjukkan oleh hasil elektroforesis pita RNA buffy coat yang lebih jelas dan konsisten. Masa penyimpanan buffy coat dan whole blood pada suhu −80°C sampai dengan 3 bulan tidak mempengaruhi kuantitas dan kemurnian RNA, namun mempengaruhi kualitas RNA.
Co-Authors Fahmita, Karin Dhia Gatot Soegiarto Laksmi Wulandari Mahdi, Bagus Aulia Nur Mahmudah Ramadhani Nurhidayat, Annisa Rina Susilowati Suhda, Saihas Winarti, Retno Yustina Andwi Ari Sumiwi