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All Journal Jurnal Sain Veteriner Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Teknologi Informasi dan Ilmu Komputer Biomedika Majalah Obat Tradisional The Indonesian Biomedical Journal Indonesian Journal of Chemistry Communications in Science and Technology GANEC SWARA Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education JURNAL ANALIS LABORATORIUM MEDIK Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Jurnal Literasi Pendidikan Fisika (JLPF) Jurnal Algoritma

Rina Susilowati

Department Of Histology And Cell Biology, Faculty Of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 396601

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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EVALUASI EFEK ANTI-DIABETES MELITUS EKSTRAK TERPURIFIKASI Andrographis paniculata (Burm. f.) Nees DAN ANDROGRAFOLID DENGAN PARAMETER INDEKS HOMA-IR Pramono, Suwidjiyo; Nugroho, Agung Endro; Adriawan, Ignatius Ryan; Andrie, Mohamad; Susilowati, Rina
Majalah Obat Tradisional Vol 19, No 1 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Diabetes mellitus (DM) tipe 2 dipicu oleh diet tinggi lemak dan fruktosa (DTLF) dan diawali dengan resistensi insulin dan hiperinsulinemia kompensatori, yang dapat dilihat dari indeks homeostatic model assessment â insulin resistance (HOMA-IR). Salah satu tanaman tradisional yang dapat digunakan untuk mengatasi DM adalah Andrographis paniculata (Burm. f.) Nees, dengan senyawa aktif utama andrografolid. Penelitian ini bertujuan untuk mengetahui efek ekstrak terpurifikasi A. paniculata dan andrografolid pada indeks HOMA-IR tikus Wistar dengan DTLF. Penelitian bersifat kuasi-eksperimental dan analisis data menggunakan uji Kruskal-Wallis. Hasil menunjukkan bahwa ekstrak terpurifikasi A. paniculata dan andrografolid dapat menurunkan indeks HOMA-IR, dan penurunan terbesar (82,05%) dihasilkan oleh ekstrak terpurifikasi A. paniculata dengan dosis 1303,8 mg/kgBB.

PERBANDINGAN DETEKSI PROLIFERASI SEL DENGAN IMUNOHISTOKIMIA PCNA DAN BrdU PADA PANKREAS TIKUS Pakaya, David; Setyowati, Wiwit; Susilowati, Rina
Biomedika Vol 12, No 2 (2020): Biomedika Agustus 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i2.10641

ABSTRAKPankreas merupakan jaringan labil karena terdiri dari sel-sel asinar yang selalu berproliferasi sepanjang hidupnya untuk menggantikan sel yang rusak. Proliferasi tersebut dapat diidentifikasi dengan pemeriksaan histologis, yaitu mikroteknik dengan pewarnaan imunohistokmia (IHC). Penelitian ini bertujuan untuk membandingkan deteksi proliferasi sel dengan IHC anti-PCNA dan anti-BrdU pada pankreas. Tikus diinjeksi BrdU secara intraperitoneal sebanyak 4 kali dengan selang waktu 3 jam dengan dosis BrdU 0,125 ml, 0,375 ml, 0,250 ml, dan 0,250 ml dan dibiarkan selama 1 malam. Jaringan pankreas dipotong sepanjang Â± 5 mm dan direndam dalam fiksatif paraformaldehid 4%, kemudian dibuat blok paraffinnya. Blok parafin diiris dengan ketebalan 6Âµm untuk pengecatan hematoksilin eosin (HE) dan 4 Âµm untuk pengecatan IHC. Pengecatan IHC dengan antibodi anti PCNA (antibodi monoklonal PCNA 1;1000 dalam PBS). Pengecatan IHC dengan antibodi anti BrdU menggunakan antibodi primer (antibodi anti BrdU 1:1000). Pengecatan dianalisis secara kualitatif. IHC menggunakan antibodi anti-PCNA dan anti-BrdU menunjukkan adanya penyebaran warna coklat gelap yang menandakan proliferasi sel aktif, dimana sel yang terekspresi pada BrdU lebih sedikit dibandingkan PCNA. Â Penelitian ini menyimpulkan bahwa proliferasi sel pankreas dapat terekspresi melalui perwarnaan IHC dengan antibodi anti PCNA dan anti BrdU.Kata kunci: Imunohistokimia, PCNA, Brdu, ProliferasiÂ ABSTRACTÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â The pancreas is a labile tissue because it consists of acinar cells which proliferate throughout their lives to replace damaged cells. The proliferation can be identified by histological examination, that is microtechnics with immunohistochmia (IHC) staining. This study aims to compare the detection of cell proliferation with anti-PCNA and anti-BrdU IHC in the pancreas. The rats were injected BrdU intraperitoneally 4 times with an interval of 3 hours with a dose of BrdU 0.125 ml, 0.375 ml, 0.250 ml, and 0.250 ml and left for 1 night. Pancreatic tissue were necropsy along Â± 5 mm and soaked in 4% paraformaldehyde fixative, then made paraffin block. The paraffin block was sliced to a thickness of 6 Âµm for hematoxylin eosin (HE) and 4 Î¼m for IHC painting. IHC used anti PCNA antibodies (PCNA monoclonal antibody 1; 1000 in PBS) and anti BrdU antibodies (anti BrdU primary antibodies 1: 1000). Staining was analyzed qualitatively. IHC with anti-PCNA and anti-BrdU antibodies showed the spread of dark brown which indicates active cell proliferation, whereas cells expressed in BrdU are less than PCNA. This study concluded that pancreatic cell proliferation can be expressed through the coloring of IHC with anti-PCNA and anti-BrdU antibodies.Keyword: Immunohistochemistry, PCNA, Brdu, Proliferation

PEMANFAATAN HEWAN COBA PADA PENELITIAN NEURODEGERATIF Pakaya, David; Susilowati, Rina
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 6 No. 2 (2020)
Publisher : Universitas Tadulako

Penyakit neurodegeneratif berhubungan dengan kerusakan bahkan kematian sel-sel saraf progresif. Untuk mempelajarinya diperlukan suatu kondisi serupa dari penyakit manusia pada hewan coba. Famili muridae merupakan hewan coba yang paling sering digunakan. Penelitian ini bertujuan untuk Mengetahui berbagai metode pengkondisian hewan coba telah dikembangkan dan digunakan dalam penelitian penyakit neurodegenratif. Dilakukan penelusuran literatur secara on-line dengan menggunakan search engine PubMed dengan kata kunci "animal model" AND "neurodegenerative". Berbagai metode pengkondisian hewan coba tersebut dapat dilakukan dengan cara merekayasa genetika, model cedera ataupun induksi bahan kimiawi dengan berbagai metode.

Optimization of Tissue Decellularization Method Based on Macroscopic and Microscopic Observation in The Sheep Peripheral Nerves Fajar Shodiq Permata; Rina Susilowati; Rini Dharmastiti; Muhammad Mirandy Pratama Sirat Mirandy Pratama Sirat; Pipin Dwi Kartikasari
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Animal graft tissue (xenograft) was developed to replace the limited supply of human graft tissue (allograft). Peripheral nerve graft tissue is needed to replace the damage. Swine is the most developed source of tissue donor for the preparation of acellular tissue because a lot of livestocks population and its extracellular matrix components similar to human collagen. Swine xenograft development would be an obstacle in Indonesia because of socio-cultural so that sheep used as swine donor replacement. Sheep cartilage tissue acellular induced less human inflammatory mediators than swine. Xenograft is necessary to decellularizebefore implantation into human that results in only extracellular matrix. However, decellularization process varies depending on the species and methods so that it is needed a preliminary study to get the best decellularization method for sheep peripheral nerve specimens. Five samples of sheep Ischiadicus nerves were conducted decellularization various processes and one sample of fresh nerve (control). Decellularization methods were 24 hours shaking, tissue soaking and perfusion for both 14 days and 17 days, respectively. Decellularization solution was 0.1% SDS-EDTAin PBS. Post decellularization, samples were observed macroscopically, fixed, HE staining of histopathologic examination for microscopic examination. The data were analyzed descriptively. At the macroscopic observation of post decellularization tissue showed white, and samples of soaking method showed softer consistency than that of shaking and perfusion. Microscopic examination showed that samples were not being completed decellularization at shaking method. There was destruction of collagen fibers of perineurium and endoneurium in 17 days of soaking and perfusion methods, while 14 days soaking and perfusion methods showed that samples were being completed decellularization and both perineurium and endoneurium collagen were still good. Conclusion of this study is that 14 days soaking and perfusion decellularization methods are better than that of 24 hours shaking method and 17 days of soaking and perfusion methods for sheep decellularization peripheral nerve.

The percentage of macrophage numbers in rat model of sciatic nerve crush injury Satrio Wicaksono; Muhammad Ghufron; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 01 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTExcessive accumulation of macrophages in sciatic nerve fascicles inhibits regeneration of peripheral nerves. The aim of this study is to determine the percentage of the macrophages inside and outside of the fascicles at the proximal, at the site of injury and at the distal segment of rat model of sciatic nerve crush injury. Thirty male 3 months age Wistar rats of 200-230 g were divided into sham-operation group and crush injury group. Termination was performed on day 3, 7, and 14 after crush injury. Immunohistochemical examination was done using anti CD68 antibody. Counting of immunopositive and immunonegative cells was done on three representative fields for extrafascicular and intrafascicular area of proximal, injury and distal segments. The data was presented as percentage of immunopositive cells. The percentage of the macrophages was significantly increased in crush injury group compared to the sham-operated group in all segments of the peripheral nerves. While the percentage of macrophages outside fascicle in all segments of sciatic nerve and within the fascicle in the proximal segment reached its peak on day 3, the percentage of macrophages within the fascicles at the site of injury and distal segments reached the peak later at day 7. In conclusions, accumulation of macrophages outside the nerve fascicles occurs at the beginning of the injury, and then followed later by the accumulation of macrophages within nerve fascicles

Correlation of methylation of toll-like receptor 4 (TLR4) and interleukin-6 (IL6) promoter with insulin resistance in obese adolescents Ratih Feraritra Danu Atmaja; Dian Caturini Sulistyonigrum; Emy Huriyati; Ahmad Hamim Sadewa; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 1 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Chronic inflammation can lead to insulin resistance in obesity. Increased mRNA expressionof inflammatory markers such as toll-like receptor 4 (TLR4) and interleukin 6 (IL6) werereported both in the tissue and the peripheral blood of obese adolescent and correlatedwith insulin resistance. DNA methylation surrounding TSS region is known to regulate thelevel of a gene expression. The aim of the study was to investigate the methylation statusof TLR4 and IL6 promoter in peripheral blood of obese adolescent and its correlation toinsulin resistance. This was a cross sectional study with observational analytic approached.Fifty adolescents with obesity from Yogyakarta Special Region, aged 15-18 years,z-score value >2 SD, no experience of any acute infections within 2 weeks and signed ininformed consent, were selected in this study. Subjects with diabetic mellitus, incompletedata and DNA concentration of <20 μg/mL were excluded. DNA samples were extractedfrom peripheral blood of the subjects. The bisulfite converted DNA was analyzed formethylation level with direct sequencing. Correlation analysis using Spearman test wasperformed with significance value was set at p<0.05. No methylation in TLR4 promoterwas detected in all subjects. The methylation level of IL6 was transformed to categoricalvariable. Four (8%) subjects with insulin resistance and 2 (4%) without insulin resistancehad methylation on a CpG site. Methylation of IL6 was not statisticaly different betweeninsulin resistance and non-resistance adolescent (p=0.635). The significant correlationwas not found between methylation level in IL6 promoter with insulin resistance (HOMAIR)(r=0.051). Cell specific of methylation mechanism, characteristic of subjects andtypes of samples were suspected causing those results. In conclusion, no correlationbetween methylation levels of TLR4 promoter and IL6 with insulin resistance in obeseadolescent was observed in this study.

Review of immune responses correlated with COVID-19 outcomes: the fight, debacle and aftermath in the Indonesian context. Dian Eurike Septyaningtrias; Jajah Fachiroh; Dewi Kartikawati Paramita; Dewajani Purnomosari; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 3 (2020): Special Issue: COVID-19
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

In the current pandemic, the highly contagious nature of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) leads to an enormous burden for the global health care system and creates challenging socioeconomic problems. Respiratory mucosa, the main entrance of SARS-CoV-2 infection, are equipped with an innate immune defense system as the initial response against infection. Activation of the adaptive immune system facilitates viral clearance as well as providing immunological memory for prevention from subsequent exposure. However, despite repeated efforts at implementing appropriate interventions, severe and fatal cases are continuing to occur and reports of recurrent cases need clarification. Host factors may contribute to the severity of the diseases while viral immune evasion is a common phenomenon leading to severe outcomes and recurrent infection. Discussions of immunological-based tests for screening, herd immunity, along with the possible advantages or potentially futile efforts of development of vaccine and alternative immunotherapy have become a part of daily household conversations. In this review, evidence of innate and adaptive immune responses or lack of them, and immunological problems relevant for SARS-CoV-2 will be summarized. Finally, perspectives for future studies especially in the Indonesian population will be sketched.

PERBANDINGAN DETEKSI PROLIFERASI SEL DENGAN IMUNOHISTOKIMIA PCNA DAN BrdU PADA PANKREAS TIKUS David Pakaya; Wiwit Setyowati; Rina Susilowati
Biomedika Vol 12, No 2 (2020): Biomedika Agustus 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i2.10641

ABSTRAKPankreas merupakan jaringan labil karena terdiri dari sel-sel asinar yang selalu berproliferasi sepanjang hidupnya untuk menggantikan sel yang rusak. Proliferasi tersebut dapat diidentifikasi dengan pemeriksaan histologis, yaitu mikroteknik dengan pewarnaan imunohistokmia (IHC). Penelitian ini bertujuan untuk membandingkan deteksi proliferasi sel dengan IHC anti-PCNA dan anti-BrdU pada pankreas. Tikus diinjeksi BrdU secara intraperitoneal sebanyak 4 kali dengan selang waktu 3 jam dengan dosis BrdU 0,125 ml, 0,375 ml, 0,250 ml, dan 0,250 ml dan dibiarkan selama 1 malam. Jaringan pankreas dipotong sepanjang ± 5 mm dan direndam dalam fiksatif paraformaldehid 4%, kemudian dibuat blok paraffinnya. Blok parafin diiris dengan ketebalan 6µm untuk pengecatan hematoksilin eosin (HE) dan 4 µm untuk pengecatan IHC. Pengecatan IHC dengan antibodi anti PCNA (antibodi monoklonal PCNA 1;1000 dalam PBS). Pengecatan IHC dengan antibodi anti BrdU menggunakan antibodi primer (antibodi anti BrdU 1:1000). Pengecatan dianalisis secara kualitatif. IHC menggunakan antibodi anti-PCNA dan anti-BrdU menunjukkan adanya penyebaran warna coklat gelap yang menandakan proliferasi sel aktif, dimana sel yang terekspresi pada BrdU lebih sedikit dibandingkan PCNA. Penelitian ini menyimpulkan bahwa proliferasi sel pankreas dapat terekspresi melalui perwarnaan IHC dengan antibodi anti PCNA dan anti BrdU.Kata kunci: Imunohistokimia, PCNA, Brdu, Proliferasi ABSTRACT The pancreas is a labile tissue because it consists of acinar cells which proliferate throughout their lives to replace damaged cells. The proliferation can be identified by histological examination, that is microtechnics with immunohistochmia (IHC) staining. This study aims to compare the detection of cell proliferation with anti-PCNA and anti-BrdU IHC in the pancreas. The rats were injected BrdU intraperitoneally 4 times with an interval of 3 hours with a dose of BrdU 0.125 ml, 0.375 ml, 0.250 ml, and 0.250 ml and left for 1 night. Pancreatic tissue were necropsy along ± 5 mm and soaked in 4% paraformaldehyde fixative, then made paraffin block. The paraffin block was sliced to a thickness of 6 µm for hematoxylin eosin (HE) and 4 μm for IHC painting. IHC used anti PCNA antibodies (PCNA monoclonal antibody 1; 1000 in PBS) and anti BrdU antibodies (anti BrdU primary antibodies 1: 1000). Staining was analyzed qualitatively. IHC with anti-PCNA and anti-BrdU antibodies showed the spread of dark brown which indicates active cell proliferation, whereas cells expressed in BrdU are less than PCNA. This study concluded that pancreatic cell proliferation can be expressed through the coloring of IHC with anti-PCNA and anti-BrdU antibodies.Keyword: Immunohistochemistry, PCNA, Brdu, Proliferation

EVALUASI EFEK ANTI-DIABETES MELITUS EKSTRAK TERPURIFIKASI Andrographis paniculata (Burm. f.) Nees DAN ANDROGRAFOLID DENGAN PARAMETER INDEKS HOMA-IR Ignatius Ryan Adriawan; Mohamad Andrie; Rina Susilowati; Suwidjiyo Pramono; Agung Endro Nugroho
Majalah Obat Tradisional Vol 19, No 1 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Diabetes mellitus type 2 was induced by high fat diet and fructose. The insulin resistance and hyperinsulinemia compensatory can be measured by index homeostatic model assessment – insulin resistance (HOMA-IR). Andrographis paniculata (Burm. f.) Nees is a traditional plant can be used to treat diabetes mellitus and the main active compound of this plant is Andrographolide. The objective of this study is to evaluate the effect of purified extract of Andrographis paniculata (Burm. f.) Nees and andrographolide on HOMA-IR index of High fat diet and fructose induced Wistar Rats. This study is quasi-experimental and data analysis was using Kruskal-Wallis test. The result showed that purified extract of Andrographis paniculata and andrographolide decreased HOMA-IR index. Purified extract of Andrographis paniculata 1303,8 mg/kg decreased HOMA-IR index by 82,05 %.

High VEGF Level is Produced by Human Umbilical Cord- Mesenchymal Stem Cells (hUC-MSCs) in Amino Acid-Rich Medium and under Hypoxia Condition Veronika Maria Sidharta; Elizabeth Henny Herningtyas; Christine Ayu Lagonda; Dilafitria Fauza; Yuyus Kusnadi; Rina Susilowati; Ginus Partadiredja
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.457

BACKGROUND: Secretome production by stem cells depends on their culture conditions such as oxygen concentration and the composition of the culture media. In this study, we investigated the secretion of neurotrophic growth factors of human umbilical cord mesenchymal stem cells (hUC-MSCs) in amino acid-rich culture medium and under hypoxic condition.METHODS: hUC-MSCs were cultured in normoxic and various hypoxic (1%, 5%, 10%) conditions in an amino acid-rich culture medium. The end-point parameters (cell proliferation and survival, cell morphology and growth factor secretion) were measured at 3 time-points (48 hours, 72 hours and 96 hours). ELISA-based methods were used for neurotrophic factors detection, including neurotrophic growth factor (NGF), vascular endothelial factor (VEGF), and brain-derived neurotrophic factor (BDNF).RESULTS: NGF secretion was not detectable at any time points both in normoxia and hypoxia. BDNF secretion under normoxia was induced at 48 h time point and reached the highest level at an average of 181.9±13.01 pg/mL at 96 hours, whereas hypoxia exposure to hUC-MSCs only induced the BDNF secretion at low level. VEGF secretion was barely detectable in normoxic condition. However, VEGF secretion reached the highest level at an average of 7707.55±2110.85 pg/mL in 5% hypoxia at 96 hours.CONCLUSION: Combination of amino acid-rich culture medium and hypoxia condition dramatically induced high VEGF secretion by hUC-MSCs, especially at 5% hypoxia, induced mild BDNF secretion and had no effect toward NGF secretion.KEYWORDS: human umbilical cord mesenchymal stem cells, neurotrophic growth factor, amino acid-rich, hypoxia

Co-Authors Adi Saputra Adika Zhulhi Arjana Agung Endro Nugroho Agung Endro Nugroho Agustin, Vita Ahmad Hamim Sadewa Anak Agung Istri Sri Wiadnyani Andwi Ari Sumiwi Arief Nurrochmad Christine Ayu Lagonda David Pakaya Dewajani Purnomosari Dewajani Purnomosari, Dewajani Dewi Kartikawati Paramita Dian Caturini Sulistyonigrum Dian Eurike Septyaningtrias Dilafitria Fauza Elizabeth Henny Herningtyas Emhandyksa, Medycha Emy Huriyati Fajar Shodiq Permata Fifteen Aprila Fajrin Ginus Partadiredja Ignatius Ryan Adriawan Ignatius Ryan Adriawan, Ignatius Ryan Indah Soesanti Jajah Fachiroh Jajah Fachiroh, Jajah Julkarnain, M Mardinata, Erwin Mohamad Andrie Muhammad Ghufron Muhammad Mirandy Pratama Sirat Ninda Devita, Ninda Pipin Dwi Kartikasari Ramadhani Nurhidayat, Annisa Ratih Feraritra Danu Atmaja Rini Dharmastiti Santoso, Fendi Satrio Wicaksono Suhda, Saihas Suwidjiyo Pramono Suwidjiyo Pramono Umi S. Intansari Ida Savitri Laksanawati Veronika Maria Sidharta Wibowo, Ghina Tamima Widyaningtyas, Febrina Siska Winarti, Retno Wiwit Setyowati Yuliani, Aska Muta Yuyus Kusnadi

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