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Rizka Tamania Saptari
Indonesian Oil Palm Research Institute
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Optimasi sistem kultur dan media untuk peningkatan tinggi tunas in vitro kelapa sawit Masna Maya Sinta; Rizka Tamania Saptari; Imron Riyadi; Sumaryono Sumaryono
Menara Perkebunan Vol. 91 No. 1 (2023): 91 (1), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i1.511

Abstract

Optimization of in vitro shoot growth is necessary to shorten the culture time and produce vigorous oil palm plantlets. This research was conducted to determine the best media and culture techniques to accelerate in vitro shoot growth of oil palm. Shoots of oil palm derived from somatic embryogenesis were grown on DF media with two culture systems (solid medium and double-layer) combined with hormone treatments (0.5-1 mg L-1 giberellin/GA3 and 0.5 mg L-1 Benzyl amino purine/BAP or thidiazuron/ TDZ). Further optimization was conducted using different bottle closures (polypropylene screw caps and plastic wraps) and macronutrients (standard or double concentrations). This research was conducted using a completely randomized design (CRD), with each treatment consisting of 5 bottle replications and each bottle consisting of 5 shoots. The results showed that media with double-layer system combined with GA3 (0.5-1 mg L-1) and TDZ (0.5 mg L-1) gave the highest shoot height increment. The use of double-strength macronutrient media combined with screw bottle caps increased shoot height (136-167 %) and decreased shoot tip necrosis (0-24 %). Plastic wrap bottle caps increased shoot tip necrosis (STN), while doubling macronutrients reduced STN. The growth of oil palm shoots began to slow down after 5 weeks of culture. In conclusion, the optimal conditions for in vitro shoot growth of oil palm were at usage of double-layer media added with GA3 0.5-1 mg L-1, TDZ 0.5 mg L-1, and double macronutrients on bottle jars with polypropylene screw caps.
Sterilization method of contaminated oil palm plantlets affects the survival rate during acclimatization Masna Maya Sinta; Lailia Zubaidah; Rizka Tamania Saptari; Imron Riyadi; Galuh Wening Permatasari; Riza Arief Putranto; Annisa A Aksa; Larasati D Mahardhika; Yuli Setiawati; Hayati Minarsih; Ernayunita Ernayunita
Menara Perkebunan Vol. 91 No. 2 (2023): 91 (2), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i2.551

Abstract

Contamination in the in vitro culture is a critical problem causing the failure of seed production. Contamination in the oil palm plantlet is detrimental, considering that oil palm propagation is difficult and takes a long time. This research aimed to study the effect of sterilization during acclimatization of the contaminated oil palm plantlets by fungi on viability and to determine the optimum viability achieved from the contaminated materials. The materials used were contaminated plantlets of oil palm with roots, four leaves, and a height of about 17 cm. The plantlets were removed from the tube and cleaned with running tap water, then were sterilized, with treatments P1: soaking in benomyl-mancozeb-sodium hypochlorite and mannitol and rinsing with aquadest, P2: soaking in benomyl-mancozeb, P3: soaking in mancozeb. Cleaning plantlets under running tap water was carried out as a control treatment. The results showed that at 10 weeks after acclimatization, the survival rate of plantlets in each treatment (P1, P2, and P3) was significantly higher than that of the control. Sterilization methods affect the time new leaves emerge, leaf condition after sterilization treatment, and shoot height. The lowest fungal contamination after treatments was found in P2, followed by P3. After 3 months, plantlet survival rate decreased, with the highest survival rate in treatment P3 (32.3%) followed by treatment P2 (22.5%). In conclusion, acclimatization of contaminated oil palm plantlets can be carried out using a suitable sterilization treatment. Sterilization affects the survival rate and growth of in vitro-contaminated oil palm plantlets during acclimatization.
Callus induction and regeneration of date palm (Phoenix dactylifera L.) cv. Zambli through somatic embryogenesis from four layers of young leaves explant Masna Maya Sinta; Rizka Tamania Saptari; Imron Riyadi; Sumaryono Sumaryono
Menara Perkebunan Vol. 92 No. 2 (2024): 92(2), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i2.588

Abstract

The Zambli variety of date palm shows potential for cultivation in tropical regions, as its fruits are edible during the Rutab stage. However, large-scale production of Zambli seedlings presents a significant challenge. In vitro propagation offers a solution for producing large quantities of clonal planting material. This study focuses on inducing callus formation from the four-layered shoot tips of young leaves and regenerating these calli into plantlets through somatic embryogenesis. Explants were cultured on a modified MS medium with 10, 50, or 100 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 1 or 3 mg L-1 N6-(2-isopentenyl)adenine (2-iP). Embryo maturation was performed on the same medium without 2,4-D, while a hormone-free medium was used for plantlet regeneration. The results indicated that the highest callus induction occurred from the younger leaf layer (layer 1) in the medium containing 100 mg L-1 2,4-D and 1 mg L-1 2-iP, achieving a callus formation rate of 82.3%. Successful callus induction was achieved from the first, second, and third layers of young leaves. Somatic embryo maturation and plantlet regeneration were also completed, producing vigorous, well-rooted plantlets. Additionally, the development of date palm cv. Zambli in vitro culture through somatic embryogenesis was confirmed through histological analysis.
Biochemical and gene expression analysis for the selection of elite oil palm ortets Irma Kresnawaty; Ludwinardo Putra; Heni Rahmawati; Zulhan Arif; Masna Maya Sinta; Rizka Tamania Saptari; Fauziatul Fitriyah; Heri Adriwan Siregar; Imron Riyadi
E-Journal Menara Perkebunan Vol. 94 No. 1 (2026): 94(1), 2026
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v94i1.676

Abstract

The rising global demand for palm oil necessitates innovative strategies to accelerate yield improvement and ensure sustainable production. Conventional propagation methods are limited by long regeneration cycles, making tissue culture a more practical alternative that relies on elite ortet identification. This preliminary study aimed to evaluate crude palm oil quality, leaf metabolite profiles, and gene expression as early indicators for selecting elite ortets. Mature fruits were used to assess oil yield, free fatty acid, and β-carotene contents, while leaves were analyzed for chlorophyll and total phenolic content. Based on biochemical screening, three candidate ortets were further evaluated for the expression of acetyl-CoA carboxylase (ACCase) and phytoene synthase (PSY) genes. Oil yield ranged from 77-83%, FFA 5-9%, and β-carotene 200-450 mg kg-1 CPO across candidates. Candidate 3.7 exhibited the highest oil yield and elevated ACCase expression, while candidate 6.12 showed the highest β-carotene content and PSY expression. Candidate 5.7 demonstrated relatively low FFA and favorable chlorophyll levels. These results suggest an association between ACCase expression and oil yield, as well as between PSY expression and β-carotene accumulation. Collectively, these findings support a proof-of-concept for an integrated profiling strategy to select elite ortets for advanced tissue culture propagation.
Co-Authors Annisa A Aksa Ernayunita Ernayunita Fauziatul Fitriyah Galuh Wening Permatasari Hayati Minarsih Heni Rahmawati Heri Adriwan Siregar Imron Riyadi Irma Kresnawaty Lailia Zubaidah Larasati D Mahardhika Ludwinardo Putra Masna Maya Sinta Masna Maya Sinta Riza Arief Putranto Sumaryono Sumaryono Yuli Setiawati Zulhan Arif