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Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 Î¼L/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.

Potensi antibakteri teripang Timba Kolong (Holothuria sp.) Kepulauan Mentawai Sumatera Barat Arma, Utmi; Prestya, Pebrian Diki; Busman, Busman
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 3 (2017): Desember
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Sumber senyawa bioaktif yang diyakini memiliki aktivitas antikanker yang kuat salah satunya adalah teripang. Teripang Timba Kolong (Holothuria sp.) merupakan spesies yang dominan di Kepulauan Mentawai Sumatera Barat Indonesia. Tujuan penelitian ini adalah untuk menentukan aktivitas antibakteri Holothuria sp. Kepulauan Mentawai Sumatera Barat. Metode: Penelitian ini adalah penelitian laboratorium eksperimental. Pengukuran aktivitas antibakteri teripang Holothuria sp. digunakan metode Kirby-Bauer dengan mengukur zona hambat terhadap Streptococcus viridans pada karies gigi. Absorbsi tiap sumuran diukur dengan spectrophotometer microplate reader pada panjang gelombang 570 nm. Penelitian aktivitas antibakteri dengan post-test only control group design dan metode disk difusion. Bakteri Streptococcus viridans dibuat suspensi dengan konsentrasi 0,625%; 1,25%; 2,5%; 5%; 10% dan 20% disiapkan dengan pelarut dimethil sulfoksida (DMSO). Hasil: Hampir semua sampel menunjukkan aktivitas antibakteri terhadap Streptococcus viridans dengan konsentrasi yang paling tinggi 0,625% yaitu 7,598 Â± 0,8427 mm. Simpulan: Ekstrak Holothuria sp. memiliki aktivitas antibakteri terhadap pertumbuhan Streptococcus viridans, maka berpotensi untuk dikembangkan sebagai sumber baru antibiotik.Introduction: For a long time, sea cucumber was believed of having powerful antibacterial potency. Timba kolong sea cucumber (Holothuria sp.) is a dominant species in Mentawai Islands, West Sumatra. The objective of this study was to determine the antibacterial activity of Holothuria sp. of Mentawai Islands West Sumatra. Methods: The methods used in this study was experimental laboratory. Antibacterial activity of Holothuria sp. was determined by using Kirby-Bauer methods by measuring the inhibitory zone of Holothuria sp. extract against Streptococcus viridans on caries. The absorption of each well was measured with a spectrophotometer microplate reader at a wavelength of 570 nm. Antibacterial activity was tested with post-test only control group design and disk difusion methods. Streptococcus viridans bacteria were made into suspension with concentration of 0.625%; 1.25%; 2.5%; 5%; 10% and 20%, prepared with dimethyl sulphoxide (DMSO) solvent. Results: Almost all samples of Holothuria sp. extracts were showed antibacterial activity against Streptococcus viridans, with the highest inhibitory zone was found in concentration of 0.625%, which was 7,598 Â± 0.8427 mm. Conclusion: Extract of Holothuria sp. has powerful antibacterial activity against the growth of Streptococcus viridans, therefore, the extract of Holothuria sp. potentially developed as new source of antibiotics.

Uji zona hambat ekstrak metanol teripang putih (holothuria scabra) mentawai terhadap Streptococcus sanguinis pada Stomatitis Aftosa Rekuren secara in vitro: studi eksperimental Rahmah, Aisha Fatihatur; Arma, Utmi; Lestari, Citra; Edrizal, Edrizal; Zia, Hanim Khalida
Padjadjaran Journal of Dental Researchers and Students Vol 8, No 1 (2024): Februari 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v8i1.52551

ABSTRAKPendahuluan: Stomatitis aftosa rekuren (SAR) atau sariawan merupakan ulser berbentuk oval atau bulat yang sakit dan terjadi secara berulang, serta dapat sembuh secara spontan dengan atau tanpa pengobatan. Streptococcus sanguinis (S. sanguinis) bentuk initial L forms banyak ditemukan pada penderita SAR dan dapat memperparah kondisi SAR. Salah satu hewan yang banyak dimanfaatkan sebagai bahan untuk pengobatan alternatif adalah teripang putih atau Holothuria scabra (H. scabra) yang berasal dari kepulauan Mentawai. Tujuan penelitian ini adalah menganalisis daya hambat ekstrak metanol teripang putih (H. scabra) terhadap S. sanguinis yang banyak ditemukan pada penderita Stomatitis Aftosa Rekuren secara in vitro. Metode: Jenis penelitian eksperimental laboratoris dengan rancangan penelitian posttest only control group design. Sampel pada penelitian ini adalah ekstrak metanol teripang putih dari pulau Siberut, kepulauan Mentawai, yang diperoleh dalam keadaan sudah kering di kota Padang dan bakteri S. sanguinis ATCC 10556 yang diperoleh dari Laboratorium Penelitian Antar Universitas (PAU) Universitas Gadjah Mada, Yogyakarta. Terdapat 5 kelompok perlakuan, yaitu pengujian dengan konsentrasi ekstrak metanol teripang putih (H. scabra) 2, 4, dan 8%, serta kontrol positif (chlorhexidine 0,2%) dan kontrol negatif. Masing-masing kelompok perlakuan diulang sebanyak 5 kali sehingga besar sampel menjadi total 25 pengulangan. Data yang diperoleh diolah menggunakan uji Independent Sample (T-Test). Hasil: Terdapat zona hambat ekstrak metanol teripang putih (H. scabra) terhadap S. sanguinis pada stomatitis aftosa rekuren yang diujikan secara in vitro pada konsentrasi 8% dengan sig 0,001 atau < 0,05, sebesar 0,81 mm, sedangkan pada konsentrasi 2 dan 4% tidak terdapat zona hambat. Simpulan: Ekstrak metanol teripang putih (H. scabra) Mentawai memiliki zona hambat pada konsentrasi 8%, tetapi dalam kategori lemah (weak) dan kurang efektif jika dibandingkan dengan kontrol positif yaitu chlorhexidine 0,2%KATA KUNCI: stomatitis aftosa rekuren, s. sanguinis, h. scabra, zona hambat, ekstrak teripangInhibition zone test of methanol extract of mentawai white sea cucumber (holothuria scabra) against streptococcus sanguinis in recurrent aphthous stomatitis in vitro: experimental studyABSTRACTIntroduction: Recurrent aphthous stomatitis (SAR) or canker sores are painful oval or round ulcers that occur recurrently and can heal spontaneously with or without treatment. Streptococcus sanguinis (S. sanguinis) plays a role in aggravating SAR in patients and mostly found in the of initial L forms. One of the animals that is widely used as an ingredient for alternative medicine is white sea cucumber or Holothuria scabra (H. scabra) from the Mentawai islands. The purpose of this study was to analyze the inhibition zone of white sea cucumber (H. scabra) methanol extract against S. sanguinis in recurrent aphthous stomatitis (in vitro study). Methods: Laboratory experimental and posttest only control group designs were used in this study. The samples in this study were a dry form of methanol extract of white sea cucumber from Siberut island, Mentawai islands obtained in Padang city. The S. sanguinis ATCC 10556 bacteria obtained from the Inter-University Research Laboratory (PAU) of Gadjah Mada University, Yogyakarta. There were 5 treatment groups, namely 2, 4, and 8%, of white sea cucumber (H. scabra) methanol extract, as well as positive control (0.2% chlorhexidine) and negative control. Each treatment group was repeated 5 times so that the sample size was a total of 25 repetitions. The data obtained was processed using the Independent Sample test (T-Test). Results: The obtained zone of inhibition of the 8% methanol extract of white sea cucumber (H. scabra) against S. sanguinis was 0.81 mm, with a significance of 0.001 (<0.05), while at concentrations of 2% and 4% there were no zone of inhibition. Conclusion: Methanol extract of Mentawai white sea cucumber (H. scabra) has an inhibition zone at the best concentration of 8%, with a weak category against S. sanguinis bacteria and less effective when compared to the 0.2% chlorhexidine.KEY WORDS: recurrent aphthous stomatitis, s. sanguinis, h. scabra, inhibition zone, sea cucumber extract.

Aktivitas antijamur ekstrak daun mangga (Mangifera indica L.) terhadap pertumbuhan Candida albicans pada basis akrilik alat ortodonti lepasan: studi eksperimental Kintaka, Malvina Aaliyya; Arma, Utmi; Alamsyah, Yenita
Padjadjaran Journal of Dental Researchers and Students Vol 8, No 1 (2024): Februari 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v8i1.52756

Efektivitas penggunaan aplikasi digital dental calculator terhadap durasi waktu pengukuran gingival index : studi eksperimental Wulandari, Ayu; Arma, Utmi; Ningrum, Valendriyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 2 (2024): Agustus 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i2.52913

ABSTRAK Pendahuluan: Permasalahan kesehatan gigi dan mulut yang sering terjadi di Indonesia salah satunya adalah gingivitis yang ditandai dengan gusi bengkak dan mudah berdarah. Gingivitis merupakan inflamasi yang terjadi pada gingiva yang disebabkan karena adanya plak yang menempel pada tepi gingiva. Tingkat keparahan gingivitis dapat diukur dengan gingival index (GI) oleh Loe dan Silness dan untuk memonitoring keadaan ini seringkali dilakukan survei. Selama ini perhitungan hasil survei di Indonesia masih dilakukan secara manual menggunakan kertas dan pena, namun seiring dengan perkembangan teknologi telah dikembangkan digital dental calculator sebagai media yang digunakan untuk mengkalkulasikan hasil pengukuran GI secara cepat dan akurat. Penelitian ini bertujuan untuk menganalisis efektivitas durasi waktu pengukuran GI menggunakan aplikasi digital dental calculator. Metode: Penelitian ini bersifat quasi experiment dengan rancangan desain posttest only group design. Teknik pengambilan sampel dilakukan dengan cara accidental sampling dengan total 65 responden yang memenuhi kriteria inklusi dan eksklusi penelitian. Data dianalisis menggunakan SPSS versi 20 menggunakan uji Mann Whitney. Hasil: Rerata durasi waktu menggunakan kalkulasi manual adalah 260,74 detik dengan simpangan baku ±30,06, sementara durasi waktu dengan menggunakan aplikasi dental calculator lebih singkat yakni rerata 105,69 detik dengan simpangan baku ±20,85. Hasil uji Mann Whitney menunjukkan adanya perbedaan durasi waktu yang signifikan p 0,000 (p<0,05) antara penggunaan aplikasi digital dental calculator dibandingkan dengan kalkulasi hasil survei secara manual dalam melakukan pengukuran GI. Simpulan: Penggunaan aplikasi digital dental calculator dapat mempersingkat waktu pengukuran skor GI. The effectiveness of using a digital dental calculator application on the duration of calculating the gingival index: experiment Introduction: One of the common dental and oral health issues that often occurs in Indonesia is gingivitis, characterized by swollen easily bleeding gums. Gingivitis is an inflammation of the gingiva caused by plaque accumulation along the gingival margin. The severity of gingivitis can be measured using the Gingival index (GI) by Loe and Silness, and this condition is often monitored through surveys. Traditionally, survey results in Indonesia have been calculated manually using paper and pen. However, with technological developments, a digital dental calculator has been developed to quickly and accurately calculate GI measurements. This study aims to determine the effectiveness of the digital dental calculator in reducing the time required to measure GI. Methods: This research is a quasi-experiment with a post-test only group design. The sampling technique was carried out by convenience sampling with a total of 65 respondents who met the inclusion and exclusion criteria of the study. Data were analyzed using SPSS version 20 using the Mann Whitney test with a significance level of p <0.05. Results: The average time taken using manual calculation was 260.74 seconds with a standard deviation of ±30.06, while the time taken using the dental calculator application was significantly shorter, with an average of 105.69 seconds and a standard deviation of ±20.85. The Mann Whitney test results showed a significant difference in time duration p= 0.000 (p <0.05) between the use of the digital dental calculator and manual calculation in measuring GI. Conclusion: The use of digital dental calculator application significantly reduces the time required for GI score measurement.

Uji zona hambat ekstrak metanol teripang pandan (Stichopus variegatus semper) terhadap bakteri Porphyromonas gingivalis Arma, Utmi; Mailiza, Fitria; Anggestia, Wulan; Azira, Faiza Nur
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 10, No 1 (2024)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.93476

Oral Squamous Cell Carcinoma (OSCC) adalah jenis kanker yang berasal dari sel epitel skuamosa di mukosa yang seringkali bersifat infiltratif dan metastatik. OSCC dapat terjadi di semua bagian rongga mulut. Etiologi OSCC belum diketahui secara pasti, namun terdapat faktor predisposisi seperti infeksi bakteri, salah satunya adalah Porphyromonas gingivalis. Porphyromonas gingivalis adalah bakteri normal di rongga mulut yang berperan dalam berbagai penyakit, seperti karsinoma dan penyakit periodontal. Pengobatan OSCC sangat bervariasi, termasuk pengobatan herbal yang menggunakan hewan. Salah satu hewan yang sering digunakan dalam pengobatan herbal adalah Stichopus variegatus semper atau teripang pandan. Teripang pandan mengandung senyawa aktif yang dapat berfungsi sebagai agen antikanker. Tujuan dari penelitian ini adalah untuk menentukan zona hambat ekstrak metanol dari teripang pandan terhadap Porphyromonas gingivalis. Jenis penelitian ini adalah eksperimen laboratoris dengan desain penelitian Post test only control group. Metode yang digunakan dalam penelitian ini adalah disk difusi dengan 5 perlakuan dan 5 ulangan menggunakan konsentrasi 5%, 6%, 7%, serta kontrol positif dan kontrol negatif, yang dianalisis dengan uji One Way Anova. Hasil yang diperoleh menunjukkan adanya zona hambat ekstrak metanol dari teripang pandan terhadap bakteri Porphyromonas gingivalis dengan nilai p < 0.05. Kesimpulan dari penelitian ini adalah bahwa ekstrak metanol dari teripang pandan mampu menghambat pertumbuhan dan perkembangan bakteri Porphyromonas gingivalis, dengan ekstrak terbaik pada konsentrasi 7% yang tergolong moderat.

Hubungan siklus menstruasi dengan stomatitis aftosa rekuren Arma, Utmi; Bakar, Abu; Yosmiryanti, Melin
Padjadjaran Journal of Dental Researchers and Students Vol 7, No 1 (2023): Februari 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v7i1.45586

ABSTRAKPendahuluan: Stomatitis Aftosa Rekuren yang dikenal dengan istilah apthae atau canker sore merupakan suatu lesi ulserasi yang terjadi berulang pada mukosa mulut. Etiologi SAR sampai saat ini belum diketahui secara pasti, namun ada faktor predisposisi yang berhubungan dengan terjadinya SAR, salah satunya adalah siklus menstruasi. Siklus menstruasi adalah tanda proses kematangan organ reproduksi yang dipengaruhi oleh hormon tubuh. Siklus menstruasi terjadi dalam 4 fase yaitu fase menstruasi, fase folikuler, fase ovulasi, dan fase luteal. Tujuan penelitian ini menganalisis hubungan siklus menstruasi dengan SAR. Metode: Jenis penelitian observasional analitik menggunakan desain cross sectional, penelitian dilakukan di Kampung Jawa, Kota Solok, Sumatra Barat pada bulan September 2021 hingga Maret 2022 dengan 94 responden yang diambil secara accidental sampling. Pengambilan data dilakukan dengan memberikan kuesioner/google form kepada perempuan berumur 15-24 tahun. Analisis data dilakukan dengan uji Chi-Square. Hasil: Sebagian besar responden negatif mengalami SAR pada siklus menstruasi yaitu sebanyak 54 responden (57,4%) dan diikuti responden yang positif mengalami SAR pada siklus menstruasi sebanyak 40 responden (42,6%).Hasil uji menunjukan hubungan bermakna antara siklus menstruasi dengan SAR (p=0,153). Simpulan: Tidak terdapat hubungan antara siklus menstruasi dengan terjadinya SAR. Terdapat lebih banyak responden yang negatif mengalami SAR pada siklus menstruasi dibandingkan responden yang positif mengalami SAR pada siklus menstruasi.KATA KUNCI: Siklus, menstruasi, stomatitis aftosa, rekuren, wanitaRelationship between menstrual cycle and recurrent aphthous stomatitisABSTRACTIntroduction: Recurrent Aphthous Stomatitis (RAS), also known as apthae or canker sores is an ulcerated lesion that occurs repeatedly on the oral mucosa. The etiology of RAS is not yet known for certain, but there are predisposing factors associated with the occurrence RAS, one of them is the menstrual cycle. The menstrual cycle is a sign of the maturation process of the reproductive organs which is influenced by the body's hormones. The menstrual cycle occurs in 4 phases, namely the menstrual phase, the follicular phase, the ovulation phase, and the luteal phase. The purpose of this study was to determine the relationship between the menstrual cycle and RAS. Methods: The type of this research is an analytic observational study using a cross-sectional design, the research was conducted in Kampung Jawa, Solok City, West Sumatra from September 2021 to March 2022 with 94 respondents who were taken by accidental sampling. Data collection was carried out by giving questionnaires/google forms to women aged 15-24 years to obtain data related to menstrual cycle and RAS. Data analysis was performed by Chi-Square test. Results: Most of the respondents are negative respondents to experiencing RAS during their menstrual cycle amounting to 54 respondents (57.4%), and followed by positive respondents experiencing RAS in the menstrual cycle amounting to 40 respondents (42.6%). The test results show a significant relationship between the menstrual cycle and RAS (p = 0.153). Conclusion: There is no relationship between the menstrual cycle and the occurrence of RAS. There are more respondents who negatively experience RAS in the menstrual cycle than positive respondents experiencing RAS in their menstrual cycle.KEY WORDS: Cycle, menstruation, aphthous stomatitis, recurrent, women

Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March 2017
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 μL/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.

The Effectiveness of Education Using Virtual Reality Video Media on Oral Health Knowledge in Elementary School Students 10 Sungai Sapih, Padang City DM, Hafizah Oulya; Arma, Utmi; Ningrum, Valendriyani
Journal of Indonesian Dental Association Vol 6 No 2 (2023): October
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v6i2.1040

Introduction: Virtual reality (VR) is a technology that creates a simulated version of the real world. In recent years, VR has been increasingly utilized as a tool in education due to its potential to enhance the learning experience. One of the factors that causes oral health problems is the low level of knowledge of individual children. Objectives: this study aims to determine the effectiveness of education using virtual reality video media on dental and oral health knowledge in students of SDN 10 Sungai Sapih, Padang. Method: This is quasi-experimental research with a one-group pre-test post-test design. Convenience sampling was used to select 40 participants who met the inclusion criteria. Statistical analysis to determine the effectiveness of the use of VR media in increasing dental health knowledge using the Wilcoxon non-parametric test. Results: The study found that the average student knowledge score increased from 11.30 to 13.20 after using VR media for education. The Wilcoxon test results indicate a significant difference before and after the VR media intervention, with a p value of 0.000 (<0.05). Conclusion: The study concludes that the use of virtual reality video media in education is effective in improving oral health knowledge among students of SDN 10 Sungai Sapih, Padang City. VR media is very innovative to be implemented in the classroom, however it requires readiness from teachers and schools to support the success of the media.

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