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All Journal Jurnal Kedokteran Brawijaya Jurnal Ilmiah Kedokteran Wijaya Kusuma Calvaria Medical Journal
Budi Arief Waskito, Budi Arief
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Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health

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EXERCISE STRESS TESTING : WHEN, HOW, AND WHAT IT MEANS? Waskito, Budi Arief
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 1, No 1 (2012): Edisi Juli 2012
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Exercise stress testing (EST) is a sensitive and informative examination of the cardiovascular response to exercise. Exercise is a common physiological stress used to elicit cardiovascular abnormalities not present at rest and to determine the adequacy of cardiac function. The electrocardiogram is the most common parameter used to evaluate the ischemic response during exercise. Exercise electrocardiography is one of the most frequent noninvasive modalities used to determine the likelihood and extent of coronary artery disease. The test is also used to estimate prognosis, to determine functional capacity, and to evaluate the effects of therapy. The indications, contraindications, protocol, when to terminate, interpretation of the result, and spesific clinical applications will be reviewed in this paper.
Cause of Change on Carotid Intima-media Thickness Waskito, Budi Arief
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 6, No 1 (2017): Edisi Maret 2017
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Atherosclerosis causes significant morbidity and mortality. Carotid intima-media thickness (CIMT) can predict the onset of cardiovascular and ischemic stroke in the future. CIMT, the measurement of atherosclerosis, is determined in vivo using carotid ultrasound. CIMT is associated with traditional cardiovascular risk factors such as age, sex, race, smoking habits, alcohol consumption, exercise habits, blood pressure, dyslipidemia, dietary patterns, medications used, glycemia, hyperuricemia, anthropometric parameters associated with obesity. In addition, CIMT is also associated with novel risk factors, ie genotypes, cardiovascular anthropometry parameters, rheumatoid arthritis, immunologic diseases, inflammatory cytokines, lipid peroxidation, chemocyte anthropometric parameters, infectious diseases, vitamin D, matrix metalloproteinases, and other diseases
Identification of ACE2 and TMPRSS2 Gene Polymorphisms in Covid-19 Survivors with Hypertension and Diabetes Mellitus using the rhAmp PCR Method Setijowati, Eva Diah; Suprapti, Herni; Waskito, Budi Arief; Wulandari, Retno Dwi
Jurnal Kedokteran Brawijaya Vol. 33 No. 4 (2025)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2025.033.04.1

Abstract

The Covid-19 pandemic has caused significant global mortality and morbidity. Covid -19 infection is caused by the SARS-CoV-2 virus, which requires a receptor on the host cell to enter, primarily Angiotensin-Converting Enzyme 2, encoded by the ACE2 gene, and Transmembrane Serine Protease 2, encoded by the TMPRSS2 gene. Several factors can influence Covid-19 mortality and morbidity, including gender, comorbidities such as diabetes mellitus (DM), obesity, cardiovascular diseases, and genetic factors. ACE2 and TMPRSS2 polymorphisms are believed to worsen the condition of Covid-19 patients. This study aimed to identify polymorphisms in the ACE2 (rs2285666 G>C) and TMPRSS2 (rs2070788 A>G) in  Covid-19 survivors with hypertension and diabetes mellitus. The total sample was 30 hypertension and diabetes mellitus   patients treated at Islamic Hospital Surabaya. A total of 3 ml of blood was drawn from the median cubital vein and placed into an EDTA vacutainer. DNA extraction was performed using the High Pure PCR Template Preparation Kit (Roche). Identification of ACE2 and TMPRSS2 polymorphisms was performed using the rhAmp SNP genotyping method with the LightCycler® system (Roche 480II). Genotyping results for ACE2 showed that 55.2% of patients had the GG (wild-type) with a G allele frequency of 68.9%. Genotyping for the TMPRSS2 showed that 60% of patients had the GG (mutant-type) with a G allele frequency of 78.3%. During Covid-19 infection, neither GG genotype (ACE2) and the GG genotype (TMPRSS2) exhibited severe symptoms.
Pengaruh Gaya Hidup Pada Wanita Pekerja Pabrik Dan Bukan Pekerja Pabrik Terhadap Hipertensi Pada Usia 40-60 Tahun mawar, mawar; Waskito, Budi Arief; Soekanto, Ayly
Calvaria Medical Journal Vol 3 No 2 (2025): Edisi Desember 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/j3ezr178

Abstract

Introduction: As time goes by, women's work is not only centered at home, but also they are involved in activities outside the home such as working. Working and non-working women have different lifestyles and can be directly or indirectly exposed to risk factors that can cause hypertension. Because of this phenomenon, the authors want to examining effect lifestyle on women who work in factories and women who are not factory workers on hypertension in the 40-60 Year Age Group in the January - March 2024 Period in Sidokepung Village, Sidoarjo. Methods: This study is a quantitative approach with a cross-sectional design and qualitative in the form of interviews. The study population was workers and not factory workers with an age group of 40-60 years in Sidokepung Village, Sidoarjo with a sample taken for this study of 60 workers who met the inclusion and exclusion criteria, and analysis with Chi Square test. Results: Most of the respondents who worked as factory workers, experienced hypertension. The risk factors for hypertension are mostly due to risk factors for physical activity. Other risk factors that researchers raised in this study such as risk factors for high salt diet, stress, and obesity not association with hypertension in factory workers in Sidokepung Village, Sidoarjo. Conclusion: The results showed that hypertension was higher in women factory workers in Sidokepung Village, Sidoarjo, where the risk factor for hypertension in women factory workers in Sidokepung Village, Sidoarjo was physical activity.
Hubungan Kadar Kolesterol, Asam Urat dan Gula Darah dengan Kejadian Hipertensi pada Pasien Prolanis Usia 50-75 Tahun di Puskesmas Paron Waskito, Budi Arief; Soekanto, Ayly; Putri, Silvia Oktavioni
Calvaria Medical Journal Vol 3 No 2 (2025): Edisi Desember 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/z7647q63

Abstract

Background: The chronic disease service program is designed for all BPJS Kesehatan members who suffer from chronic diseases including type 2 diabetes mellitus and hypertension. Hypertension is diagnosed when a person has systolic blood pressure ≥ 140 mm Hg and/or diastolic blood pressure ≥ 90 mm Hg. Cholesterol, uric acid and blood sugar are triggers for non-communicable diseases, one of which is hypertension. Based on the background above, researchers are interested in conducting a study that aims to determine the relationship between cholesterol, uric acid and blood sugar levels with the incidence of hypertension in Prolanis patients aged 50-75 years at the Paron Health Center, Ngawi Regency in the period January-March 2024. Methods: This study is a cross-sectional study with an observational analysis method using primary data. The population that became the subject of this study were Prolanis hypertension patients aged 50-75 years at the Paron Health Center, Ngawi Regency with a sample taken for this study of 52 patients who had met the inclusion criteria. The data analysis of this study used the chi square test. Results: Based on gender, Prolanis hypertension patients aged 50-75 years in women are 79.5% high cholesterol levels, and male patients are 46.2% high cholesterol levels, in women 56.4% have high uric acid levels and male patients are 53.8% have high uric acid levels, in women 7.7% high blood sugar levels and male patients are 7.7% have high blood sugar levels. Based on obesity status, Prolanis hypertension patients aged 50-75 years who are obese are 89.2% high cholesterol levels and non-obese are 26.7% high cholesterol levels, obese patients are 64.9% high uric acid levels and non-obese patients are 33.3% high uric acid levels, obese patients are 5.4% high blood sugar levels and non-obese patients are 13.3% high blood sugar levels. Conclusion: Based on gender, there is a relationship between cholesterol levels, with the incidence of hypertension in Prolanis patients aged 50-75 years. However, there is no relationship between uric acid levels and blood sugar levels with the incidence of hypertension in Prolanis patients aged 50-75 years. Based on obesity status, there is a relationship between cholesterol and uric acid levels with the incidence of hypertension in Prolanis patients aged 50-75 years. However, there is no relationship between blood sugar levels with the incidence of hypertension in Prolanis patients aged 50-75 years.
Co-Authors Ayly Soekanto Herni Suprapti, Herni Mawar Mawar, Mawar Putri, Silvia Oktavioni Setijowati, Eva Diah Wulandari, Retno Dwi