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All Journal LINK Jaringan Laboratorium Medis Borneo Journal of Medical Laboratory Technology Borneo Community Health Sevice Journal Jurnal Pengabdian Kesehatan Gema Lingkungan Kesehatan
Nanik Aryani Putri
Department Of Medical Laboratory Technology, Politeknik Kesehatan Kementerian Kesehatan Semarang, Semarang, Central Java, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Tinjauan Pewarnaan Hemaktosilin-Eosin dan Periodic Acid-Schiff terhadap Kerusakan Hati Mencit yang Diinduksi Aloksan: A Review of Hematoxylin-Eosin and Periodic Acid Schiff Staining to Assess Alloxan-Induced Liver Injury in Mice Nanik Aryani Putri; Erick Khristian; Adang Durachim
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5101

Abstract

The liver is an organ that serves several functions in the body, including glucose metabolism to provide energy to other tissues. Hepatocytes are the primary functional cells of the liver, which also regulate liver glucose release via glucose transport protein-2. Hepatocyte injury could occur from toxic substances and diseases such as Diabetes Mellitus. Alloxan is an organic compound commonly used in diabetes research as a diabetogenic agent. Alloxan causes diabetes through selective inhibition of glucose-stimulated insulin secretion and induced formation of reactive oxygen species (ROS) that promotes pancreatic beta cell necrosis. Alloxan also affects the liver's histological condition, including the hepatocellular structure and glycogen content. HE and PAS are used for evaluating this condition. However, both should be reviewed to evaluate their abilities. Mice were divided into control and test groups, each consisting of 5 mice. The test group was intervein-induced with 25, 50, and 100mg/KgBB alloxan on the second day of arrival. The mice's livers were then taken on the seventh day; tissue processing was carried out to get 20 blocks of mice's livers. Two 5-μm-thick paraffin-embedded sections from each group were stained with hematoxylin-eosin, and periodic acid Schiff, respectively. Mice's liver slides are examined microscopically for the degree of injury and glycogen concentration for further evaluation using ImageJ digital imaging application. This study found that microscopical and ANOVA tests of both staining methods successfully produced significant differences between control and various dose alloxan-induced groups of mice.
PENGENDALIAN DIABETES MELITUS MELALUI EDUKASI DAN PEMERIKSAAN KADAR GULA DARAH SEWAKTU DI PERUMAHAN ROTO KENONGO SEWON Yoki Setyaji; Iin Desminay Duri; Putri Kuniasiwi; Nanik Aryani Putri
Borneo Community Health Service Journal VOLUME 3 NOMOR 2 TAHUN 2023
Publisher : Fakultas Ilmu Kesehatan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/neotyce.v3i2.4227

Abstract

Diabetes Mellitus (DM) is one of many health problems that have an impact on productivity and reduce the quality of human resources. Diabetes is a metabolic disorder characterized by high blood glucose levels and several symptoms including polyuria, polydipsia, polyphagia and weight loss. This community service aims to increase knowledge and awareness in the prevention and management of Diabetes Mellitus and to find out the category of blood sugar levels in the Roto Kenongo Sewon Residential Community, Bantul District, Yogyakarta. The method used is socialization with lectures and discussions as well as checking the current blood sugar (GDS). The number of participants in this community service was attended by 30 participants. The results of this service found that 3 people got GDS results 200 mg/dl in the DM category. The results of education on management of DM, the community was enthusiastic and able to answer several questions during the discussion session.
Hubungan Pengetahuan, Sikap, Perilaku Terhadap Kepatuhan Minum Obat Pasien TB di Kota Semarang Mochamad Rizal Maulana; Nanik Aryani Putri; Qurrotu A’yuni Auliya; Eko Naning Sofyanita
Gema Lingkungan Kesehatan Vol. 22 No. 1 (2024): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v22i1.99

Abstract

Mycobacterium tuberculosis (M. tuberculosis) adalah bakteri yang menyebabkan penyakit menular tuberkulosis (TB) secara langsung. Menurut Organisasi Kesehatan Dunia (WHO), sekitar 10,6 juta orang di seluruh dunia terinfeksi bakteri tuberkulosis pada tahun 2021. Dalam periode 2016 hingga 2020, tiga negara dengan tingkat tuberkulosis, MDR-TB, dan tuberkulosis tertinggi/ HIV salah satunya adalah negara Indonesia. Jawa Tengah adalah salah satu wilayah Indonesia tertinggi dengan 40.528 kasus TB dan CNR hingga 110 per 100.000 penduduk. Kepatuhan pasien dalam minum obat sangat berpengaruh terhadap hasil pengobatan TB. Pasien yang tidak minum obat secara konsisten berisiko drop out dan meningkatkan resistensi obat (MDR). Penelitian bertujuan untuk melihat bagaimana sikap, perilaku, dan pengetahuan seseorang berhubungan dengan kepatuhan pengobatan pasien TB. Studi yang menerapkan metodologi potong lintang (cross-sectional) kepada 81 responden pasien TB di Puskesmas Kedungmundu dan Rowosari Kota Semarang ini bersifat kuantitatif dengan rumus perhitungan sampel slovin dari populasi sebanyak 418 orang. Data yang terkumpul kemudian diuji secara statistik menggunakan uji chi-square untuk menguji ada tidaknya hubungan antar variabel. Temuan mengungkapkan adanya hubungan yang kuat antara kepatuhan pengobatan (0,05) di Kota Semarang dan pengetahuan pasien (p=0,006), sikap (p=0,001), dan perilaku (p=0,000). Menurut temuan penelitian yang dilakukan, ada hubungan yang kuat antara kepatuhan pengobatan pasien dengan pengetahuan, sikap, dan perilaku pasien. Pasien yang secara konsisten minum obat anti TB dianggap penting dalam keefektifan program pengobatan. Penelitian selanjutnya agar dapat meningkatkan jumlah sampel dan memperlauas cakupan daerah sasaran agar dapat mendeteksi dan memberikan gambaran secara lebih luas terhadap variable yang diteliti.
Pengabdian Masyarakat dalam Rangka Pengaplikasian Penelitian: Kasiat Tanaman Obat Bidang Mikrobiologi Widodo Widodo; Sunarto Sunarto; Fauzan Ma'ruf; Nanik Aryani Putri; Sy. Didik Widiyanto
Jurnal LINK Vol 22 No 1 (2026): MEI 2026
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/link.v22i1.14709

Abstract

Morinda citrifolia is efficacious for treating several degenerative diseases such as cancer, tumors, and diabetes. These substances have been proven to be antibacterial substances due to infectious bacteria such as, Proteus morganii, Bassilus subtilis, Staphylococus aureus, Pseudomonas aeruginosa and Echercia coli. This community service aims to utilize noni fruit, ginger, and honey as a form of application for the application of research results in community outreach held in Bangetayu Wetan village. Noni fruit that has been fermented is then tested against Eschericia coli and Salmonella typhi bacteria using micro titers. The results showed that at a concentration of 25%, there was inhibition of the growth of Salmonella typhi bacteria, while for Escheria coli at a concentration of 25%, growth inhibition began to occur. With a very large concentration of the taste of this fermentation, the acidity and smell of noni that is typical for some people may find it difficult to accept. Based on the results of the community service to the community, the community welcomes the results of the community that have been distributed to the entire community properly.
PEMBERDAYAAN KADER KESEHATAN MASYARAKAT SERTA EDUKASI BAGI IBU HAMIL DAN IBU BALITA UNTUK MENCEGAH STUNTING DI PEDURUNGAN, KOTA SEMARANG Iin Desmiany Duri; Roni Afriansya; Dewi Andang Prastika; Putri Kurniasiwi; Nanik Aryani Putri
JURNAL PENGABDIAN KESEHATAN Vol. 4 No. 2 (2026): MEI
Publisher : Gayaku Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/heq49939

Abstract

Stunting masih menjadi permasalahan kesehatan masyarakat di Indonesia karena berdampak terhadap pertumbuhan fisik, perkembangan kognitif, dan kualitas sumber daya manusia di masa depan. Rendahnya pengetahuan kader kesehatan, ibu hamil, dan ibu balita mengenai pencegahan stunting dapat menghambat upaya penurunan angka stunting. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan serta memberdayakan kader kesehatan, ibu hamil, dan ibu balita terkait pencegahan stunting di Pedurungan Kota Semarang. Metode yang digunakan berupa edukasi kesehatan melalui ceramah, diskusi, dan tanya jawab yang disertai evaluasi menggunakan kuesioner pre-test dan post-test untuk mengukur perubahan tingkat pengetahuan peserta sebelum dan sesudah intervensi. Kegiatan dilaksanakan pada tanggal 25 Mei 2024 dengan melibatkan 41 peserta yang terdiri atas kader kesehatan, ibu hamil, dan ibu balita. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan peserta, ditandai dengan persentase jawaban benar yang meningkat dari 79,8% pada pre-test menjadi 90% pada post-test. Peserta juga menunjukkan pemahaman yang lebih baik mengenai penyebab, dampak, dan langkah pencegahan stunting. Pemberdayaan masyarakat melalui edukasi dan keterlibatan kader terbukti efektif dalam meningkatkan pengetahuan serta memperkuat partisipasi masyarakat dalam upaya pencegahan stunting. Kegiatan edukasi berkelanjutan dan peran aktif kader perlu terus didukung untuk mempertahankan keberhasilan program pencegahan stunting.
Analisis Hubungan Status Gizi dan Anemia pada Pasien Tuberkulosis Fase Intensif dan Lanjutan Nanik Aryani Putri; Teguh Budiharjo; Moch. Alvin 'Izzuddin; Siti Ulul Azmi
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14723

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacterium Mycobacterium tuberculosis, which enters the human body through the air. Treatment for tuberculosis involves Anti-Tuberculosis Drugs (OAT)—including pyrazinamide, rifampicin, isoniazid, ethambutol, and streptomycin—which may cause side effects that increase the risk of nutritional status changes and anemia. This study analyzes the relationship between nutritional status, anemia status, and the phase of treatment among TB patients in Semarang City.This research employed a descriptive observational design with a cross-sectional approach. Data were obtained from outpatient TB patients at the Bangetayu and Kedungmundu Public Health Centers (Puskesmas). A total of 44 adult subjects without comorbidities were recruited via purposive sampling, comprising 20 patients in the intensive phase and 24 in the continuation phase of treatment. Nutritional status was evaluated using Body Mass Index (BMI), while anemia status was assessed through hematological parameters, including hemoglobin (Hb), hematocrit (Ht), and erythrocyte indices. Statistical analysis revealed no significant correlation between anemia status or hematological parameters (Hb, Ht, erythrocyte indices) and BMI in either treatment group (p>0.05). These findings suggest that hematological profiles may be more heavily influenced by systemic inflammation resulting from tuberculosis (TB) infection or the effects of anti-TB therapy rather than nutritional status alone.Conversely, a significant correlation was identified between BMI and body weight (p<0.001), reflecting the inherent variations in patients' nutritional status. Future studies incorporating a larger sample size and additional variables—such as the duration of TB treatment, disease severity, and dietary intake—are warranted to further elucidate these findings. This study provides an initial contribution to understanding the link between TB, nutritional status, and anemia. Furthermore, platelets and body height showed trends toward significant differences, which may serve as a basis for further research and open opportunities for developing nutrition-based interventions for TB patients.
Co-Authors Adang Durachim Devi Etivia Purlinda Dewi Andang Prastika Eko Naning Sofyanita Erick Khristian Fauzan Ma’aruf Fitriani Kahar Iin Desmiany Duri Iin Desminay Duri Maulana, Mochamad Rizal Moch. Alvin 'Izzuddin Putri Kuniasiwi Putri Kurniasiwi Qurrotu A’yuni Auliya Roni Afriansya Siti Ulul Azmi Sunarto Sunarto Sy. Didik Widiyanto Teguh Budiharjo Widodo Widodo Widodo Widodo Yoki Setyaji