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All Journal Jaringan Laboratorium Medis Borneo Journal of Medical Laboratory Technology Borneo Community Health Sevice Journal Jurnal Pengabdian Kesehatan
Nanik Aryani Putri
Poltekkes Kemenkes Semarang
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Tinjauan Pewarnaan Hemaktosilin-Eosin dan Periodic Acid-Schiff terhadap Kerusakan Hati Mencit yang Diinduksi Aloksan: A Review of Hematoxylin-Eosin and Periodic Acid Schiff Staining to Assess Alloxan-Induced Liver Injury in Mice Nanik Aryani Putri; Erick Khristian; Adang Durachim
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5101

Abstract

The liver is an organ that serves several functions in the body, including glucose metabolism to provide energy to other tissues. Hepatocytes are the primary functional cells of the liver, which also regulate liver glucose release via glucose transport protein-2. Hepatocyte injury could occur from toxic substances and diseases such as Diabetes Mellitus. Alloxan is an organic compound commonly used in diabetes research as a diabetogenic agent. Alloxan causes diabetes through selective inhibition of glucose-stimulated insulin secretion and induced formation of reactive oxygen species (ROS) that promotes pancreatic beta cell necrosis. Alloxan also affects the liver's histological condition, including the hepatocellular structure and glycogen content. HE and PAS are used for evaluating this condition. However, both should be reviewed to evaluate their abilities. Mice were divided into control and test groups, each consisting of 5 mice. The test group was intervein-induced with 25, 50, and 100mg/KgBB alloxan on the second day of arrival. The mice's livers were then taken on the seventh day; tissue processing was carried out to get 20 blocks of mice's livers. Two 5-μm-thick paraffin-embedded sections from each group were stained with hematoxylin-eosin, and periodic acid Schiff, respectively. Mice's liver slides are examined microscopically for the degree of injury and glycogen concentration for further evaluation using ImageJ digital imaging application. This study found that microscopical and ANOVA tests of both staining methods successfully produced significant differences between control and various dose alloxan-induced groups of mice.
PENGENDALIAN DIABETES MELITUS MELALUI EDUKASI DAN PEMERIKSAAN KADAR GULA DARAH SEWAKTU DI PERUMAHAN ROTO KENONGO SEWON Yoki Setyaji; Iin Desminay Duri; Putri Kuniasiwi; Nanik Aryani Putri
Borneo Community Health Service Journal VOLUME 3 NOMOR 2 TAHUN 2023
Publisher : Fakultas Ilmu Kesehatan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/neotyce.v3i2.4227

Abstract

Diabetes Mellitus (DM) is one of many health problems that have an impact on productivity and reduce the quality of human resources. Diabetes is a metabolic disorder characterized by high blood glucose levels and several symptoms including polyuria, polydipsia, polyphagia and weight loss. This community service aims to increase knowledge and awareness in the prevention and management of Diabetes Mellitus and to find out the category of blood sugar levels in the Roto Kenongo Sewon Residential Community, Bantul District, Yogyakarta. The method used is socialization with lectures and discussions as well as checking the current blood sugar (GDS). The number of participants in this community service was attended by 30 participants. The results of this service found that 3 people got GDS results 200 mg/dl in the DM category. The results of education on management of DM, the community was enthusiastic and able to answer several questions during the discussion session.
PEMBERDAYAAN KADER KESEHATAN MASYARAKAT SERTA EDUKASI BAGI IBU HAMIL DAN IBU BALITA UNTUK MENCEGAH STUNTING DI PEDURUNGAN, KOTA SEMARANG Iin Desmiany Duri; Roni Afriansya; Dewi Andang Prastika; Putri Kurniasiwi; Nanik Aryani Putri
JURNAL PENGABDIAN KESEHATAN Vol. 4 No. 2 (2026): MEI
Publisher : Gayaku Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/heq49939

Abstract

Stunting masih menjadi permasalahan kesehatan masyarakat di Indonesia karena berdampak terhadap pertumbuhan fisik, perkembangan kognitif, dan kualitas sumber daya manusia di masa depan. Rendahnya pengetahuan kader kesehatan, ibu hamil, dan ibu balita mengenai pencegahan stunting dapat menghambat upaya penurunan angka stunting. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan serta memberdayakan kader kesehatan, ibu hamil, dan ibu balita terkait pencegahan stunting di Pedurungan Kota Semarang. Metode yang digunakan berupa edukasi kesehatan melalui ceramah, diskusi, dan tanya jawab yang disertai evaluasi menggunakan kuesioner pre-test dan post-test untuk mengukur perubahan tingkat pengetahuan peserta sebelum dan sesudah intervensi. Kegiatan dilaksanakan pada tanggal 25 Mei 2024 dengan melibatkan 41 peserta yang terdiri atas kader kesehatan, ibu hamil, dan ibu balita. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan peserta, ditandai dengan persentase jawaban benar yang meningkat dari 79,8% pada pre-test menjadi 90% pada post-test. Peserta juga menunjukkan pemahaman yang lebih baik mengenai penyebab, dampak, dan langkah pencegahan stunting. Pemberdayaan masyarakat melalui edukasi dan keterlibatan kader terbukti efektif dalam meningkatkan pengetahuan serta memperkuat partisipasi masyarakat dalam upaya pencegahan stunting. Kegiatan edukasi berkelanjutan dan peran aktif kader perlu terus didukung untuk mempertahankan keberhasilan program pencegahan stunting.
Analisis Hubungan Status Gizi dan Anemia pada Pasien Tuberkulosis Fase Intensif dan Lanjutan Nanik Aryani Putri; Teguh Budiharjo; Moch. Alvin 'Izzuddin; Siti Ulul Azmi
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14723

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacterium Mycobacterium tuberculosis, which enters the human body through the air. Treatment for tuberculosis involves Anti-Tuberculosis Drugs (OAT)—including pyrazinamide, rifampicin, isoniazid, ethambutol, and streptomycin—which may cause side effects that increase the risk of nutritional status changes and anemia. This study analyzes the relationship between nutritional status, anemia status, and the phase of treatment among TB patients in Semarang City.This research employed a descriptive observational design with a cross-sectional approach. Data were obtained from outpatient TB patients at the Bangetayu and Kedungmundu Public Health Centers (Puskesmas). A total of 44 adult subjects without comorbidities were recruited via purposive sampling, comprising 20 patients in the intensive phase and 24 in the continuation phase of treatment. Nutritional status was evaluated using Body Mass Index (BMI), while anemia status was assessed through hematological parameters, including hemoglobin (Hb), hematocrit (Ht), and erythrocyte indices. Statistical analysis revealed no significant correlation between anemia status or hematological parameters (Hb, Ht, erythrocyte indices) and BMI in either treatment group (p>0.05). These findings suggest that hematological profiles may be more heavily influenced by systemic inflammation resulting from tuberculosis (TB) infection or the effects of anti-TB therapy rather than nutritional status alone.Conversely, a significant correlation was identified between BMI and body weight (p<0.001), reflecting the inherent variations in patients' nutritional status. Future studies incorporating a larger sample size and additional variables—such as the duration of TB treatment, disease severity, and dietary intake—are warranted to further elucidate these findings. This study provides an initial contribution to understanding the link between TB, nutritional status, and anemia. Furthermore, platelets and body height showed trends toward significant differences, which may serve as a basis for further research and open opportunities for developing nutrition-based interventions for TB patients.
Co-Authors Adang Durachim Dewi Andang Prastika Erick Khristian Iin Desmiany Duri Iin Desminay Duri Moch. Alvin 'Izzuddin Putri Kuniasiwi Putri Kurniasiwi Roni Afriansya Siti Ulul Azmi Teguh Budiharjo Yoki Setyaji