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Performance of F2 Generation Lines of Soybean (Glycine max. L) as Backcross Results of GHJ-4 and GHJ-5 with Ryoko as A Donor Parent Nurul Sjamsijah; Sri Rahayu; Eva Rosdiana; Putri Santika; Sepdian Luri Asmono
Journal of Applied Agricultural Science and Technology Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i4.97

Abstract

The breeding of new high-yielding varieties is one of the solutions to the low productivity of soybeans in Indonesia. Superior genotypes of ‘GHJ-4 (A)’ and ‘GHJ-5 ’ were obtained in the previous study with an average weight of 13 g/100 seeds and a potential yield of 3 tons/ha. The attempt to increase the weight and size of the seeds of ‘GHJ-4’ and ‘GHJ-5’ was continued using the backcrossing method with ‘Ryoko  (>35 g/100 seeds) as a parent donor. F1 plants were obtained with an average weight of 15 g/100 seeds. This study aimed to determine the performance of the F2 generation of backcross in terms of the number of filled pods, the weight of 100 seeds, yield per plant (g), yield per plot (kg), and potential yield per hectare (tons/ha), as well as the heritability value of the genotypes. In this particular study, the F2 seeds from F1 plants were then planted and observed. This study used a non-factorial randomized block design (RBD) with 11 soybean genotypes, each consisting of 3 replications. The results showed that the F2 generations (GHJ-4XRyoko, RyokoXGHJ -5, and GHJ-4XGHJ-5) are considered as prospective new high-yielding varieties with a potential yield of ≥ 3.5 tons/ha, which have successfully restored the trait of ‘Ryoko’ and surpassed the performance of ‘GHJ-4’ and ‘GHJ-5’ as their parents in all parameters. The heritability value of the genotypes tested belongs to the high category, on the parameters of the number of filled pods (96.937 %), yield per plant (98.102 %), the weight of 100 seeds (99.132%), yield per plot (92.605 %) and potential yield per hectare (92.257 %).  For the parameters observed, the back cross yields of harvest age were 72-74 HST, the weight of 100 grains was 17-18 grams and the yield per hectare was around 4 tons/ha. The heritability value shows that the observed traits are inherited from parents on average more than 90% and the selection that will be used is pedigree selection.
Induksi Poliploidi Menggunakan Perlakuan Kolkisin pada Anggrek Bulan (Phalaenopsis sp.) secara In Vivo Ermawati, Netty; Sjamsijah, Nurul; Syaban, Rahmat Ali; Prasetyo, Hari; Safi'i, Mohamad
Agriprima : Journal of Applied Agricultural Sciences Vol 8 No 1 (2024): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v8i1.657

Abstract

Anggrek bulan merupakan salah satu bunga yang populer di Indonesia, karena bentuk dan warnanya yang bervariasi, sehingga permintaan akan anggrek ini baik sebagai bunga hias atau bunga potong terus meningkat. Sejalan dengan meningkatnya permintaan terhadap anggrek, diperlukan upaya peningkatan kualitas anggrek, khususnya melalui perlakuan induksi mutasi untuk memperoleh keanekaragaman. Penelitian ini bertujuan untuk mengetahui pengaruh tingkat konsentrasi kolkisin (senyawa mutagen) terhadap karakter morfologi dan fisiologis (khususnya organ stomata) pada Phalaenopsis. Penelitian ini dilaksanakan pada bulan Agustus 2022 sampai dengan Januari 2023, menggunakan rancangan acak lengkap dengan perlakuan kolkisin yang terdiri dari 5 konsentrasi berbeda dengan 4 kali pengulangan. Kadar perlakuan meliputi 0 ppm (Kontrol), K1 (30 ppm), K2 (35 ppm), K3 (40 ppm), dan K4 (45 ppm). Data penelitian diuji menggunakan uji F ANOVA, apabila hasil menunjukkan berbeda nyata maka diuji lanjut menggunakan LSD dengan tingkat kesalahan 5%. Hasil penelitian menunjukkan bahwa perlakuan kolkisin 45 ppm memberikan pengaruh berbeda sangat nyata terhadap karakter morfologi seperti umur munculnya daun baru, tinggi tanaman, dan jumlah daun, serta memberikan pengaruh berbeda nyata terhadap parameter warna daun, sedangkan Karakter anatomi mempunyai pengaruh yang sangat nyata terhadap parameter kepadatan stomata. Namun perlakuan konsentrasi kolkisin tidak memberikan pengaruh nyata terhadap parameter tebal daun, persentase hidup tanaman, panjang stomata, dan lebar. Hasil penelitian menunjukkan bahwa perlakuan kolkisin 45 ppm mampu menginduksi poliploidi awal yang ditunjukkan oleh parameter jumlah stomata. Semakin tinggi konsentrasi colchicine mengakibatkan semakin rendahnya kepadatan stomata.
Time of detasseling and defoliation effect on hybrid sweet corn (Zea mays saccharata. Sturt) seed production Rahmawati, Dwi; Rahayu, Sri; Sjamsijah, Nurul; Nafisah, Siti

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v15i2.4105

Abstract

Introduction: Sweet corn (Zea mays saccharata Sturt) is one of the horticultural crops that is in great demand in Indonesia because of its distinctive sweet taste and relatively short production time. To improve the quality and quantity of hybrid sweet corn seed production, it is necessary to apply appropriate agronomic techniques, such as detasseling (removal of male flowers) and defoliation (pruning leaves). This study aims to determine the interaction between detasseling time and the number of leaves pruned on the production parameters of hybrid sweet corn. Methods: The study was conducted in Wringintelu Village, Puger, Jember, from August to December 2022, using a factorial randomized block design (RBD) with three replications. The treatments consisted of three detasseling times (48 DAP, 50 DAP, and 52 DAP) and three levels of defoliation (no defoliation, 2 leaves, and 4 leaves). Data were analyzed using ANOVA and continued with a 5% DMRT test if there was a significant effect. Results: The results showed that the interaction of detasseling time of 48 HST with 4-leaf defoliation gave the best results on the parameters of cob weight (78.55 g) and cob length (14.97 cm). Individually, the treatment of detasseling time of 48 HST produced the highest seed production per hectare of 18.44 tons, while 4-leaf defoliation produced a production of 18.17 tons per hectare. Conclusion: In conclusion, the combination of detasseling time of 48 HST and 4-leaf defoliation is the best treatment to increase the production of hybrid sweet corn seeds, so it can be a recommendation for agronomic practices to increase sweet corn productivity in Indonesia.