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All Journal METAMORFOSA Journal of Biological Sciences INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Biopendix: Jurnal Biologi, Pendidikan dan Terapan Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia Prosiding Seminar Nasional Pengabdian Kepada Masyarakat
Muhammad Taufiq Hidayat
Department of Medical Laboratory Technology, FKes, Nahdlatul Ulama University of Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Nursing Public Health Social Sciences

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Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument Muhammad Taufiq Hidayat; Endry Nugroho Prasetyo
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.1863

Abstract

RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.
Comparison of Three DNA Isolation Methods of Aspergillus Niger Adyan Donastin; Maharani Pertiwi Koentjoro; Muhamad Taufik Hidayat; Endry Nugroho Prasetyo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p07

Abstract

Polymerase Chain Reaction (PCR) is a technique that is applied to detect and test the presence of genetic material from pathogenic fungi. This method was further developed because it has a high sensitivity compared to the culture method in detecting the presence of pathogenic fungi. To perform sensitive, specific and reliable PCR-based assays, the availability of pure DNA as well as an easy-to-perform DNA extraction protocol is essential. Currently, existing protocols for DNA extraction generally require specialized kits and with the addition of enzymes. Therefore, this study was conducted to compare the quantity and quality of Aspergillus niger DNA isolation with three different methods. The stages of the research carried out included pure A. niger culture and total DNA isolation using three methods, namely the protocol according to the Wizard® Genomic DNA Purification Kit (P1) instructions, Modified Wizard® Genomic DNA Purification Kit (P2) and Monarch Genomic DNA Purification Kit NEB (P3). The results of the evaluation of DNA isolation using a spectrophotometer at a wavelength of A260/280 nm showed a ratio of 1.4, 1.8, and 1.7 at P1 and P2, and P3 respectively. The quantity obtained ranged from 210 to 305 ng/µL. The total DNA results obtained were then used for PCR testing of ITS ribosomal DNA fragments using ITS1 and ITS4 primers. Electrophorosis observation of PCR results showed that all samples produced bands with a length of 500 bp. The results of DNA isolation in the P1 method did not show bands on the agarose gel, but could be seen in the PCR product. Methods P2 and P3 showed DNA that had good quality and quality. The P2 and P3 methods use a cell destruction technique with liquid nitrogen and a combination of addition of proteinase K. The obtained protocol is expected to provide fast and good method information in the isolation of total DNA from A. niger. Keyword: Aspergillus niger, total DNA isolation, ITS1, ITS4
Antimicrobial Activity Test of Medicinal Plant Extract Using Antimicrobial Disc and Filter Paper Muhammad Taufiq Hidayat; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetyo
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7544

Abstract

Plant extracts contain secondary metabolites such as flavonoids and phenolic compounds with antibiotic activity. Antibiotic activity test can be done by using the Kirby-Bauer disc method which the antibiotic activity is indicated by the formation of a clear zone. This research was conducted using extracts of Moringa oleifera leaves and Opuntia cochenillifera cladode extracted using ethyl acetate as a solvent. Antibiotic activity test against Staphylococcus aureus bacteria was done in Mannitol Salt Agar and Luria Bertani Agar media. The Kirby-Bauer discs used are Antimicrobial Disc Oxoid® and Filter Paper. The results of this study show that the use of the Antimicrobial Disc Oxoid® and Filter Paper shows no statistical difference in clear zone results (t-value = 0,45; p-value = 0,655; with α = 95%). While the Clear Zone results on Mannitol Salt Agar gave better results than Luria Bertani Agar and were statistically significant (t-value = 2,46; p-value = 0,02; with α = 95%). These findings indicate that filter paper can be an inexpensive alternative for the antibiotic test with no significantly different result compared to commercial antibiotic disc. However, an antibiotic test against Staphylococcus aureus is better done on Mannitol Salt Agar (MSA) compared to Luria Bertani Agar (LBA).
Edukasi Kelor Antibakteri Herbal dan Pelatihan Teh Kelor untuk Warga Desa Simoangin Muhammad Taufiq Hidayat; Endah Prayekti; Maki Zamzam; Yauwan Tobing Lukiyono; Muhammad Afwan Romdloni
Jurnal Pengabdian Masyarakat Indonesia Vol 3 No 4 (2023): JPMI - Agustus 2023
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.1529

Abstract

Infeksi bakteri dan resistensi antibiotik merupakan masalah kesehatan yang serius di Indonesia. Salah satu penyebabnya adalah kurangnya pemahaman masyarakat tentang penggunaan antibiotik yang benar. Oleh karena itu, tim Pengabdian Masyarakat Program Studi Analis Kesehatan UNUSA melakukan kegiatan “Edukasi dan Diseminasi Hasil Penelitian Kelor sebagai Antibakteri Herbal serta Pelatihan Pembuatan Teh Kelor untuk Warga Desa Simoangin-angin”. Kegiatan ini bertujuan untuk meningkatkan pengetahuan dan keterampilan masyarakat tentang pencegahan dan pengobatan penyakit bakteri menggunakan obat herbal kelor. Metode yang digunakan adalah seminar dan pelatihan. Seminar membahas potensi ekstrak kelor sebagai antibakteri berdasarkan hasil penelitian tim dan penerapannya yang bijak. Pelatihan membahas cara pembuatan teh kelor dan manfaatnya sebagai alternatif pengobatan. Brosur edukasi juga dibagikan kepada peserta kegiatan. Hasil kegiatan menunjukkan bahwa ada peningkatan pemahaman masyarakat tentang kelor sebagai antibiotik herbal dan penggunaan antibiotik yang bijak, serta ada peningkatan keterampilan masyarakat dalam pembuatan teh kelor. Kegiatan ini diharapkan dapat memberikan dampak positif bagi kesehatan masyarakat Desa Simoangin-angin. Selain itu, kegiatan ini juga sejalan dengan konsep MBKM (merdeka belajar kampus merdeka) dan mendukung pencapaian IKU (indikator kinerja utama perguruan tinggi) dengan memberikan pengalaman belajar di luar kampus bagi mahasiswa dan melibatkan dosen dalam kegiatan pengabdian kepada masyarakat.
NEW PRIMER DESIGN of MULTIPLEX POLYMERASE CHAIN REACTION (PCR) for DETECTION of Escherichia Coli and Salmonella Enterica in FOOD SAMPLE Hidayat, Muhammad Taufiq; Prayekti, Endah; Lukiyono, Yauwan Tobing; Romdhoni, Muhammad Afwan; Sari, Devi Fitriana
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page17-25

Abstract

Background: Multiplex PCR techniques are used to detect multiple pathogens at the same time simultaneously. In order to achieve the specificity and sensitivity of detection, it is important to optimize the multiplex PCR method properly. Developing suitable primers and optimizing PCR temperature to boost particular genes from pathogens are key factors for this optimization. Methods: For the simultaneous detection of Escherichia coli and Salmonella enterica in food samples, this research aims to develop a set of primers for multiplex polymerase chain reaction (PCR). Using Primer-BLAST software, the study utilizes specific primer designs for the phoA gene (Eschericia coli) and invA gene (Salmonella enterica). DNA isolation has confirmed successful extraction from the two bacterial samples. PCR was performed under different conditions, including Singleplex and Multiplex PCR, using two annealing temperatures of 53oC and 50oC. Results: The results showed that this method can effectively amplify target genes and indicate their specificity and reliability at both temperature levels. Given these results, it has successfully conducted multiplex PCR using the built primer pairs. Both annealing temperatures of 50oC and 53oC can be used to perform multiplex PCR to detect E. coli and Salmonella enterica in one PCR reaction. Conclusion: Through this research, we have created a new set of Multiplex PCR Primers and an optimized multiplex PCR technique for the simultaneous detection of E coli and Salmonella enterica in food samples. The rapid and simultaneous screening of E. coli and S. enterica, which contributes to improved food safety measures and pathogen detection in the food industry, is promising with this optimized PCR approach.
Edukasi Pembuatan Teh Herbal Dan Pemeriksaan Glukosa Urine Muhammad Taufiq Hidayat; Andreas Putro Ragil Santoso; Ary Andini; Widya Novitasari; Imratul Mukarramah; Lili Susan Rahmawati
PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT Vol. 4 No. 1 (2024): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : Memaksimalkan Potensi
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/snpm.v4i1.1362

Abstract

Penggunaan bahan herbal sampai saat ini masih digunakan untuk mengurangi obat-obatan kimia, salah satu penggunaan obat herbal yaitu menggunakan teh, konsumsi the baik untuk Kesehatan namun perlu diperhatikan penggunaan gula untuk menghindari diabetes. Kegiatan pengabdian kepada masyarakat dilakukan dilakukan di RT Medokan Sawah Timur, Rungkut, Surabaya pada tahun 2024 yang dihadiri oleh 30 peserta. Metode pengabdian yang dilakukan yaitu dengan pendekatan melalui sosialisasi untuk pemahaman penyakit minum the herbal, manfaat pemahaman dilakukan dengan sosialisasi dilakukan pendekatan kembali terkait tingkat pemahaman, selanjutnya dilakukan pemeriksaan urine. Berdasarkan hasil pengabdian kepada masyarakat di RT Medokan Sawah Timur sebanyak 30 responden didapatkan hasil pemahaman tentang pentingnya teh herbal yaitu sebesar 50% persen peningkatan pemahaman setelah dilakukan sosialisasi, dan berdasarkan hasil pemeriksaan glukosa pada urine didapatkan hasil 1 orang yang positif dari 30 responden.
Co-Authors Adyan Donastin Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Endah Prayekti Endry Nugroho Prasetyo Endry Nugroho Prasetyo Imratul Mukarramah Lili Susan Rahmawati Maharani Pertiwi Koentjoro Maharani Pertiwi Koentjoro Maki Zamzam Muhammad Afwan Romdloni Prayekti, Endah Romdhoni, Muhammad Afwan Sari, Devi Fitriana Setyo Nugroho Widya Novitasari