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Biogrouting: Produksi Urease dari Bakteri Laut (Oceanobacillus sp.) Pengendap Karbonat Maharani Pertiwi, Sidratu Ainiyah Puspita Lisdiyanti; Prasetyo, Endry Nugroho
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 1 (2016): February 2016
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (808.211 KB) | DOI: 10.24002/biota.v1i1.707

Abstract

Biogrouting adalah teknologi yang mensimulasi proses diagenesis yaitu transformasi butiran pasir menjadi batuan pasir (calcarinite/sandstone).  Permasalahan penelitian ini bagaimana mengoptimasi produk urease dengan melakukan uji aktifitas, mengisolasi, mempurifikasi dan mengkarakterisasi serta mengaplikasikannya sebagai material grout. Uji aktifitas dan optimasi dilakukan dengan menumbuhkan isolat Oceanobacillus sp. pada dua variasi medium (B4 urea dan B4 urin), lima variasi pH (4-8) dan dua variasi suhu (25°C dan 29°C). Hasil uji aktifitas dan optimasi selanjutnya dipurifikasi menggunakan ammonium sulfat dan dicari titik isoelektriknya. Kemudian hasil protein presipitat dikarakterisasi menggunakan SDS-PAGE. Berdasarkan hasil penelitian diketahui bahwa aktifitas urease paling tinggi adalah 203,32 unit/ml. Urease optimal dihasilkan pada isolat yang ditumbuhkan pada B4 urea pada pH 7 suhu 25°C. Berat molekul urease yang dikarakterisasi menggunakan SDS-PAGE adalah 440 kDa, sedangkan titik isoelektriknya pada pH 6. Urease dapat dijadikan material  grout  karena memiliki kemampuan untuk melakukan sementasi pada aplikasi sederhana biogrouting.
DISEMINASI CARA HIDUP SEHAT DAN SANITASI DI KAWASAN PESISIR PULAUAN KECIL POTERAN, SUMENEP MADURA Koentjoro, Maharani Pertiwi; Masruroh, Inayatul; Isdiantoni, Isdiantoni; Prasetyo, Endry Nugroho
JMM (Jurnal Masyarakat Mandiri) Vol 5, No 3 (2021): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.837 KB) | DOI: 10.31764/jmm.v5i3.4682

Abstract

Abstrak: Kesehatan menjadi salah satu faktor penentu kualitas Sumber Daya Manusia (SDM). Di Pulau Poteran, akses pelayanan kesehatan terhalang dengan keadaan geografis dan keadaan cuaca. Kondisi ini mengakibatkan kurangnya perhatian masalah kesehatan dan kondisi sanitasi lingkungan masyarakat pesisir. Tujuan pengabdian masyarakat ini adalah diseminasi cara hidup sehat dan sanitasi kepada masyarakat di kawasan pesisir pulau Poteran, agar masyarakat (1) termotivasi untuk menjaga kebersihan lingkungan; (2) melakukan pencegahan dan penanganan secara dini terhadap penyakit; (3) mendorong peran serta, penguatan kesadaran dan kepedulian masyarakat untuk berperan aktif dalam menangani sanitasi lingkungan. Metode yang dilaksanakan pada kegiatan ini adalah penyuluhan melalui media poster pendidikan sanitasi lingkungan berbahasa Madura yang tinggal di pulau kecil di pulau Poteran. Mitra kegiatan ini adalah Yayasan Jala Tani Pertiwi. Hasil evaluasi yang dilakukan menggunakan kuesioner sebelum dan sesudah sosialisasi menunjukkan terjadi peningkatan pemahaman cara hidup sehat dan sanitasi sebesar 42%. Peningkatan pemahaman ini diharapkan mampu mengubah pola pikir dan perilaku hidup sehat masyarakat. Abstract:  Health is one of the determining factors for the quality of Human Resources (HR). In Poteran Island, access to health services is hindered by geography and weather conditions. This condition results in a lack of attention to health problems and environmental sanitation conditions in coastal communities. The purpose of this community service is the dissemination of healthy living and sanitation methods to communities in the coastal area of Poteran Island, so that people are (1) motivated to maintain a clean environment; (2) early prevention and treatment of disease; (3) encouraging participation, strengthening public awareness and concern to play an active role in dealing with environmental sanitation. The method used in this activity is to provide counseling and distribution of environmental sanitation education posters in Madurese language living on a small island on Poteran Island. The partner of this activity is the Jala Tani Pertiwi Foundation. The results of evaluations conducted using a questionnaire before and after the socialization showed an increase in understanding of healthy living and sanitation methods by 42%. This increase in understanding is expected to be able to change the mindset and behavior of healthy living in society.
PENGARUH MASA SIMPAN DAN JENIS PENGIKAT GRAFTING TERHADAP KEBERHASILAN GRAFTING SIRSAK (Annona muricata) VARIETAS RATU Tri Wahyu Hidayat; Ali Mustafa Sidauruk; Rico Hutama Sulistiyo; Buana Susilo; Lengga Nurullah Dalimartha; Eko Chandra Wiguna; Isdiantoni Isdiantoni; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetyo
Biogenesis Vol 14, No 2 (2018)
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.916 KB) | DOI: 10.31258/biogenesis.14.2.7-12

Abstract

ABSTRACTIn nursery technology, the storage of scions always requires approriate optimization of shelf life and good packing This study aims to determine the effect of shelf life and storage materials on viability of soursop (Annona muricata) var. Ratu grafting by applying randomized factorial design with 2 factors: storage time and type of packing materials, with 3 times repeatation. The duration of storage time factor consist of 5 levels: 0 day, 3 days, 5 days, 1 week and 2 weeks meanwhile packing materials factor consist of 2 levels: parafilm and polyethylene. The highest survival rate was obtained at 0 day shelf life time and parafilm as storage material, whereas the lowest one was shown at 2 weeks storage with polyethylene as storage material.Keywords: Scion, Grafting, Storage, Packing Material, Soursop
PENAMBAHAN BIOCHAR BERBASIS LIMBAH PADA INCEPTISOL TERHADAP BUDIDAYA TEMBAKAU (Nicotiana tabacumL.) VARIETAS KEMLOKO DI TEMANGGUNG Katon Sasongko Damarmoyo; Buana Susilo; Lengga Nurullah Dalimartha; Eko Chandra Wiguna; Isdianto Isdianto; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetiyo
Biogenesis Vol 14, No 2 (2018)
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (554.255 KB) | DOI: 10.31258/biogenesis.14.2.13-22

Abstract

ABSTRACTBiochar is a biological charcoal derived from incomplete combustion (pyrolysis) of agricultural residual materials that can increase carbon sustainably, retention of water and nutrients in soil. This research aimed to demonstrate the effect of adding biochar on inceptisol for Kemloko tobacco cultivation in Temanggung, Central Java, Indonesia. The data analysis of this research was using Factorial Random Design with two factors (treatment and dose). The results showed that the addition of biochar + compost increase productivity of 491.6 kg/ha comparing to the control with the higher tobacco quality. Chemical analysis of the respected soil showed that the biochar + compost treatment increase pH level up to 5.46 with the organic matter level and total nitrogen as well increased about 2.66% and 0.180% respectively.Keywords:  biochar, tobacco of kemloko variety, temanggung, soil chemical
Local Perception and Behavior in Domestic Waste Management and Water Quality of The Brantas River, Dinoyo Ward Anggayasti, Wresti L.; Al Zamzami, Ilham Misbakudin; Pramudia, Zulkisam; Dwi Susanti, Yogita Ayu; Moehammad, Khibar Syiar; Ulfa, Siti Mariyah; Efani, Anton; Koentjoro, Maharani Pertiwi; Amin, Abd Aziz; Yanuar, Adi Tiya; Salamah, Lutfi Ni’matus; Galisong, Ridwan Danuarta; Aulia, Lisa; Kurniawan, Andi
Jurnal Pembangunan dan Alam Lestari Vol. 15 No. 2 (2024): Jurnal Pembangunan dan Alam Lestari
Publisher : Postgraduate School of Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2024.015.02.04

Abstract

Waste management by society not only reflects individual responsibility but also plays a crucial role in environmental sustainability and community welfare. This research aimed to understand society’s perception and behavior regarding domestic waste and its surrounding waters. Understanding societal behavior is the key to sustainable environmental management. Direct inquiry was conducted to the sample population of 25 respondents who represented various age, education, and cultural groups in the densely populated residential area surrounding the Brantas River in Dinoyo Ward. Field observations were done to take water quality parameters and monitor the residents’ behavior regarding waste management. The results indicated that 76% of respondents know the impact of river pollution, although most did not contribute actively to reducing their domestic waste volume. About 44% of respondents never saw anyone littering the river, which may be due to the regular waste pick-up system. This was supported by water quality parameters that did not demonstrate any sign of pollution, although high turbidity was indicated. Comprehension of the results of this study is hoped to inspire the ideas of solid intervention plans to increase awareness and decrease the negative impact of domestic waste on the Brantas River in Dinoyo Ward. Keywords: society perception and behavior, domestic waste management, aquatic environment, Brantas River
Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Abstract

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.
ANTIMICROBIAL ACTIVITY TEST OF MEDICINAL PLANT EXTRACT USING ANTIMICROBIAL DISC AND FILTER PAPER AGAINST Staphylococcus aureus : CLEAR ZONE RESULTS COMPARISON Hidayat, Muhammad Taufiq; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43276

Abstract

Ekstrak tumbuhan mengandung metabolit sekunder berupa flavonoid dan senyawa fenolik yang memiliki aktivitas antibiotik. Uji aktivitas antibiotik dapat dilakukan dengan metode cakram Kirby-Bauer yang aktivitas antibiotiknya ditunjukkan dengan terbentuknya zona bening. Penelitian ini dilakukan dengan menggunakan ekstrak  daun Moringa oleifera  dan  kladode Opuntia cochenillifera  yang diekstrak menggunakan etil asetat sebagai pelarut. Uji aktivitas antibiotik terhadap  bakteri Staphylococcus aureus  dilakukan pada media Mannitol Salt Agar dan Luria Bertani Agar. Cakram Kirby-Bauer yang digunakan adalah Antimicrobial Disc Oxoid® dan Filter Paper. Hasil penelitian ini menunjukkan bahwa penggunaan Antimicrobial disc Oxoid® dan Filter Paper tidak menunjukkan perbedaan secara statistik pada hasil zona bening (t-value = 0,45; p-value = 0,655; dengan α = 95%). Sementara itu, hasil Clear Zone pada Mannitol Salt Agar memberikan hasil yang lebih baik daripada Luria Bertani Agar dan signifikan secara statistik (nilai-t = 2,46; nilai-p = 0,02; dengan α = 95%). Temuan ini menunjukkan bahwa kertas saring dapat menjadi alternatif yang murah untuk uji antibiotik dengan hasil yang tidak berbeda secara signifikan dibandingkan dengan cakram antibiotik komersial. Namun, uji antibiotik terhadap Staphylococcus aureus lebih baik dilakukan pada Mannitol Salt Agar dibandingkan dengan Luria Bertani Agar.
Alteration of the Rhizosphere Bacteria Community Respond Differently to Plant Growth Promoting Rhizobacteria in Peanut Soil’s Poteran Island Ekawati, Ida; Wati, Henny Diana; Koentjoro, Maharani Pertiwi; Sudarwati, Herni; Isdiantoni, Isdiantoni; Prasetyo, Endry Nugroho
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.3947

Abstract

Plant growth-promoting rhizobacteria (PGPR) has been detailed to affect soil microbial exercises or community composition. There is a lack of information on the degree to which PGPR as a biofertilizer concurrently influences the action, estimate, and composition of the soil microbial community. This research investigated the impacts of the timing and frequency of PGPR application as biofertilizers on the rhizosphere bacteria community in arable soil. We compared four treatments in an experimental field site, namely: one-time treatment of PGPR at the beginning of planting (P1), twice treatment of PGPR (P2), 15-days of planting (DP), and 30-DP (P3); and without treatment of PGPR (WP). A total rhizosphere bacteria community fingerprint was surveyed using ribosomal intergenic spacer analysis (RISA) using a culture-dependent and culture-independent approach. The rhizosphere bacteria community was surveyed during 80 DP. The unweighted pair-group method with arithmetic mean (UPGMA) clustering showed that the rhizosphere bacteria community in three-frequency applications of PGPR was more abundant than without PGPR application and one or two PGPR applications. This study revealed that the rhizosphere bacteria community was increased in soil with PGPR application, especially in P3, three dosages of PGPR application. Increasing the rhizosphere bacteria community could strongly influence the clay foam soil nutrient.
PENDAMPINGAN PENGUATAN TATA KELOLA PENCEGAHAN, PENANGANAN DAN PEMULIHAN KEKERASAN SEKSUAL DI SATUAN PENDIDIKAN Koentjoro, Maharani Pertiwi; Riniwati, Harsuko; Wike, Wike; Setiawati, Eti; Irawati, Ina; Arifin, Nur Hikmah; Fitriyah, Ikhrotul
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 1 (2025): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i1.27831

Abstract

Abstrak: Kekerasan seksual di lingkungan pendidikan, mengalami peningkatan jumlah kasus akibat pertumbuhan penduduk, perkembangan teknologi, dan ketimpangan gender yang belum terselesaikan. Di sisi lain, kesadaran hak asasi manusia terus meningkat. Sebagai upaya pencegahan dan penanganan yang komprehensif, Peraturan Menteri Pendidikan, Kebudayaan, Riset, dan Teknologi Nomor 46 Tahun 2023 menetapkan peraturan mengenai pencegahan dan penanganan kekerasan seksual di satuan pendidikan, termasuk sekolah umum. Tujuan dari kegiatan pengabdian ini untuk membantu meningkatkan pemahaman dan strategi pimpinan satuan pendidikan dalam upaya mencegah kasus kekerasan seksual melalui kolaborasi dengan instansi terkait. Mitra program pengabdian ini adalah guru pengajar di SMP Brawijaya Smart School, Kota Malang sebanyak 30 orang. Sebagai upaya peningkatan kapasitas, metode pengabdian dilakukan melalui diskusi, sosialisasi, dan pelatihan pencegahan serta penanganan kekerasan seksual kepada guru dan tenaga kependidikan. Kegiatan ini menunjukkan hasil positif dengan meningkatnya pemahaman peserta terkait tata kelola kekerasan seksual. Hasil post-test menunjukkan tingkat pemahaman naik sebesar 30%. Program ini diharapkan berkontribusi terhadap penguatan kebijakan pencegahan dan penanganan kekerasan seksual di lingkungan pendidikan di Malang.Abstract: Sexual violence in educational settings has increased in number of cases due to population growth, technological development, and unresolved gender inequality. On the other hand, human rights awareness continues to increase. As a comprehensive prevention and handling effort, the Minister of Education, Culture, Research and Technology Regulation No. 46 of 2023 stipulates regulations regarding the prevention and handling of sexual violence in educational units, including public schools. The purpose of this service activity is to help improve the understanding and strategies of education unit leaders in preventing cases of sexual violence through collaboration with related agencies. The partners of this service programme are thirty teacher at SMP Brawijaya Smart School in Malang City. As a capacity building effort, the service method was carried out through discussions, socialisation, and training on prevention and handling of sexual violence to teachers and education personnel. This activity showed positive results with an increase in participants' understanding of sexual violence governance. The post-test results showed that the level of understanding increased by 30%. This programme is expected to contribute to the strengthening of policies for the prevention and handling of sexual violence in the educational environment in Malang. 
The Role of Biofilm on Microplastics as A Vector for Heavy Metals in the Waters of Sendang Biru, Malang Regency Al Fatih, Ahmad Nuril Fuad; Kurniawan, Andi; Koentjoro, Maharani Pertiwi
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1092-1102

Abstract

Microplastic contamination in water provides an ideal surface for biofilm formation. It facilitates other pollutants, such as heavy metals, to attach to their surface. This interaction leads to biological and environmental problems. We tested this phenomenon by investigating the presence of microplastics in water, biofilms on surfaces, and heavy metal accumulation during September 2024. It further examines the relationship between microplastic and their contamination in fish. Samples were collected from two stations, namely Kondang Buntung Fish Auction Place (TPI) and the Indonesian Navy Post, water from Sendang Biru, using a purposive sampling method. A total of 20 liters of water and 6 Banyar fish (Rastrelliger kanagurta) specimens were analyzed. In contrast, biofilm morphology and heavy metal content were analyzed using Scanning Electron Microscopy with energy-dispersive X-ray. The result found that fibre-shaped microplastics, with fragments and filaments, dominated water samples. Microplastics 1-5 mm in size show that their colors included black, blue, red, and transparent. Five biofilm morphologies on the microplastic surfaces were identified: platelet, thin film, solid film, sporous, and diatoms. Heavy metals (Pb, Hg, Cd, Al, Mg, Fe) were detected with weight percentages ranging from 0.473 to 2.533%. Microplastics are found in the digestive tracts of fish, predominantly microplastic as filaments followed by fragments and fibers. We found that biofilm-coated microplastics accumulate heavy metals, posing a risk of pollutant transfer to aquatic organisms.
Co-Authors Adyan Donastin Afifah, Kufah Nur Ahmad Nuril Fuad Al Fatih Al Fatih, Ahmad Nuril Fuad Al zamzami, Ilham Misbakudin Alfi Muflikhah Lestari Ali Mustafa Sidauruk Alviani, Melinda Nuril Amin, Abd Aziz Andi Kurniawan Anggayasti, Wresti L. Anggraeni, Ike Listya Anthon Efani Arifin, Nur Hikmah Ashari, Fadhil Muhamad Ayuningtyas Megawati Bambang Semedi Bambang Semedi Beatriex, Cendy Kartika Buana Susilo Bunga Hidayati Dalimartha, Lengga Nurullah Devi Farah Azizah Dwi Susanti, Yogita Ayu Efani, Anton Eko Chandra Wiguna Endry Nugroho Prasetiyo Endry Nugroho Prasetyo Eti Setiawati, Eti Fitria Dina Riana Fitriyah, Ikhrotul Galisong, Ridwan Danuarta Gunawan, Fahdynia Karnira Habibah, Laila Nur Hakasmanti, Erika Hardjoko, Arief Tri Harsuko Riniwati Hashim, Jamal Hisham Herni Sudarwati Hidayat, Muhammad Taufiq Ida Ekawati Imam Santoso Ina Irawati, Ina Isdianto Isdianto Isdiantoni Isdiantoni Isdiantoni Ismail, Nik Nasri Nik Kartikaningsih, Hartati Katon Sasongko Damarmoyo Katon, Damarmoyo Sasongko Kurniawan, Muhammad Alivio Devananda Adinegara Lengga Nurullah Dalimartha Liana, Afifah Lilik Wahyuni Lisa Aulia, Lisa Masruroh, Inayatul Moch. Sahri Moehammad, Khibar Syiar Mufidah, Elya Muhammad Fathur Rouf Hasan Muhammad Taufiq Hidayat Ningtyas, Anyndia Putri Dwi Candra Nurlelih, Euis Elih Pahlevi, Reza Widhi Pramudia, Zulkisam Prasetiyo, Endry Nugroho Rico Hutama Sulistiyo Rini Yulianingsih Salamah, Lutfi Ni’matus Setyo Nugroho Shamsudin, Shamsul Bahari Sinurat, Moses Siti Asmaul Mustaniroh Siti Mariyah Ulfa Sri Sudaryanti, Sri Supriyadi Supriyadi SURYADI, DEDE Susilo, Buana Taufiq Hidayat Tri Wahyu Hidayat Wati, Henny Diana Wiguna, Eko Chandra Wike, Wike Yanuar, Adi Tiya YOGA DWI JATMIKO Yusuf Efendi