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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences JBIO: jurnal biosains (the journal of biosciences) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pembangunan dan Alam Lestari AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pendidikan IPA (JPPIPA) JMM (Jurnal Masyarakat Mandiri) Bioscientist : Jurnal Ilmiah Biologi BIOGENESIS Proceeding Biology Education Conference Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat JAPI: Jurnal Akses Pengabdian Indonesia

Maharani Pertiwi Koentjoro

Unknown Affiliation

Author-ID : 299236

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Biogrouting: Produksi Urease dari Bakteri Laut (Oceanobacillus sp.) Pengendap Karbonat Maharani Pertiwi, Sidratu Ainiyah Puspita Lisdiyanti; Prasetyo, Endry Nugroho
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 1 (2016): February 2016
Publisher : Universitas Atma Jaya Yogyakarta

Biogrouting adalah teknologi yang mensimulasi proses diagenesis yaitu transformasi butiran pasir menjadi batuan pasir (calcarinite/sandstone).Â Permasalahan penelitian ini bagaimana mengoptimasi produk urease dengan melakukan uji aktifitas, mengisolasi, mempurifikasi dan mengkarakterisasi serta mengaplikasikannya sebagai material grout. Uji aktifitas dan optimasi dilakukan dengan menumbuhkan isolat Oceanobacillus sp. pada dua variasi medium (B4 urea dan B4 urin), lima variasi pH (4-8) dan dua variasi suhu (25Â°C dan 29Â°C). Hasil uji aktifitas dan optimasi selanjutnya dipurifikasi menggunakan ammonium sulfat dan dicari titik isoelektriknya. Kemudian hasil protein presipitat dikarakterisasi menggunakan SDS-PAGE. Berdasarkan hasil penelitian diketahui bahwa aktifitas urease paling tinggi adalah 203,32 unit/ml. Urease optimal dihasilkan pada isolat yang ditumbuhkan pada B4 urea pada pH 7 suhu 25Â°C. Berat molekul urease yang dikarakterisasi menggunakan SDS-PAGE adalah 440 kDa, sedangkan titik isoelektriknya pada pH 6. Urease dapat dijadikan materialÂ groutÂ karena memiliki kemampuan untuk melakukan sementasi pada aplikasi sederhana biogrouting.

DISEMINASI CARA HIDUP SEHAT DAN SANITASI DI KAWASAN PESISIR PULAUAN KECIL POTERAN, SUMENEP MADURA Koentjoro, Maharani Pertiwi; Masruroh, Inayatul; Isdiantoni, Isdiantoni; Prasetyo, Endry Nugroho
JMM (Jurnal Masyarakat Mandiri) Vol 5, No 3 (2021): Juni
Publisher : Universitas Muhammadiyah Mataram

Abstrak: Kesehatan menjadi salah satu faktor penentu kualitas Sumber Daya Manusia (SDM). Di Pulau Poteran, akses pelayanan kesehatan terhalang dengan keadaan geografis dan keadaan cuaca. Kondisi ini mengakibatkan kurangnya perhatian masalah kesehatan dan kondisi sanitasi lingkungan masyarakat pesisir. Tujuan pengabdian masyarakat ini adalah diseminasi cara hidup sehat dan sanitasi kepada masyarakat di kawasan pesisir pulau Poteran, agar masyarakat (1) termotivasi untuk menjaga kebersihan lingkungan; (2) melakukan pencegahan dan penanganan secara dini terhadap penyakit; (3) mendorong peran serta, penguatan kesadaran dan kepedulian masyarakat untuk berperan aktif dalam menangani sanitasi lingkungan. Metode yang dilaksanakan pada kegiatan ini adalah penyuluhan melalui media poster pendidikan sanitasi lingkungan berbahasa Madura yang tinggal di pulau kecil di pulau Poteran. Mitra kegiatan ini adalah Yayasan Jala Tani Pertiwi. Hasil evaluasi yang dilakukan menggunakan kuesioner sebelum dan sesudah sosialisasi menunjukkan terjadi peningkatan pemahaman cara hidup sehat dan sanitasi sebesar 42%. Peningkatan pemahaman ini diharapkan mampu mengubah pola pikir dan perilaku hidup sehat masyarakat.Â Abstract:Â Health is one of the determining factors for the quality of Human Resources (HR). In Poteran Island, access to health services is hindered by geography and weather conditions. This condition results in a lack of attention to health problems and environmental sanitation conditions in coastal communities. The purpose of this community service is the dissemination of healthy living and sanitation methods to communities in the coastal area of Poteran Island, so that people are (1) motivated to maintain a clean environment; (2) early prevention and treatment of disease; (3) encouraging participation, strengthening public awareness and concern to play an active role in dealing with environmental sanitation. The method used in this activity is to provide counseling and distribution of environmental sanitation education posters in Madurese language living on a small island on Poteran Island. The partner of this activity is the Jala Tani Pertiwi Foundation. The results of evaluations conducted using a questionnaire before and after the socialization showed an increase in understanding of healthy living and sanitation methods by 42%. This increase in understanding is expected to be able to change the mindset and behavior of healthy living in society.

Produksi Kitosan Secara Enzimatik oleh Bacillus Sampah Perikanan Afifah, Kufah Nur; Koentjoro, Maharani Pertiwi; Prasetyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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The ability of Bacillus sp. has been developed by isolation bacteria from fisheries waste to synthesis chitin deaetylase. This microorganism secretes extracellular chitin deacetylase and does not involve cell lysis. Chitin deacetylase has roles as bioconversion which converts chitin to chitosan with deacetylation N-acetylglucosamine residue in chitin. The bioconversion process of chitin into chitosan in this study is occured by Bacillus sp. B3, Bacillus sp. B5, Bacillus sp. B6, and Bacillus sp. B12 through deacetylation enzymatic which is isolated from fisheries waste. The protein component of crude enzyme was measured using Bradford methods with BSA (Bovine Serum Albumin) as standard. The enzyme was purified by ammonium sulfate precipitation. Chitin deacetylase was characterized by measuring isoelectric point, enzyme activity, and protein component. While the fungtional groups of chitosan had characterized by FTIR and measured degree of deacetylation chitosan. The highest enzyme activity was achieved by Bacillus sp. B12 through ammonium sulphate purification at 75-90% fraction is 345.21U/ml with protein content is 0.0512 mg/ml. Chitin deacetylase from fourth Bacillus sp. has an isoelectric point at pH 5. The highest degree of deacetylation chitosan is 67% and the termination of acetyl groups had confirmed by IR spectra.

Penambahan Biochar Berbasis Limbah pada Inceptisol terhadap Budidaya Tembakau (Nicotiana tabacum (L.)) Varietas Kemloko di Temanggung Katon, Damarmoyo Sasongko; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Isdiantoni, Isdiantoni; Koentjoro, Maharani Pertiwi; Prasetiyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Biochar is a biological charcoal derived from incomplete combustion (pyrolysis) of agricultural residualmaterials that can increase carbon sustainably, retention of water and nutrients in soil. This research aimed todemonstrate the effect of adding biochar on inceptisol for Kemloko tobacco cultivation in Temanggung, CentralJava, Indonesia. The data analysis of this research was using Factorial Random Design with two factors(treatment and dose). The results showed that the addition of biochar + compost increase productivity of 491.6kg/ha comparing to the control with the higher tobacco quality. Chemical analysis of the respected soil showedthat the biochar + compost treatment increase pH level up to 5.46 with the organic matter level and total nitrogenas well increased about 2.66% and 0.180% respectively.

Model Manajemen Terpadu Pertanian Hortikultura Organik pada Lahan Sempit Suryadi, Dede; Megawati, Ayuningtyas; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Isdiantoni, Isdiantoni; Koentjoro, Maharani Pertiwi; Prasetiyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Integrated management of organic farming is an agriculture management system with an effort in developing the organic basis of cropping system to improve soil health, and pest & disease controlling to achieve sustainable productivity. Integrated management model of organic horticulture can be done alternatively on narrow land thereby intercropping and companion of several crops in order to improve yield. For instance, planting of different plants families such as herbs & flower in one area such as kenikir and tagetes to reduce the population of pest and to improve natural enemies population.

Pengaruh Formulasi Penambahan Biochar terhadap Produksi Tanaman Tembakau Varietas K326 Cross Creek Seed USA. di Lahan Kering Kabupaten Bojonegoro Pahlevi, Reza Widhi; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Isdiantoni, Isdiantoni; Koentjoro, Maharani Pertiwi; Prasetiyo, Endry Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Tobacco plant has high value and strongly sensitive to physical and chemical conditions of soil condition. One alternative to increase tobacco production is modify the soil using biochar accompanied with other organic materials such as fertilizer. This research aimed to determine the best formulation on addition of biochar to the yield of tobacco (Nicotiana tabacum) plants var. K326. The study was conducted in Balen Subdistrict, Bojonegoro District, East Java, Indonesia with Randomized Block Design consisting of 5 treatments. Prior aplication the biochar was formulated with compost, OCF (Organic Compound Fertilizer), and agricultural waste. The formulation is intended to allow biochar having adequate nutrient content to increase tobacco yield. Biochar treatment formulated with OCF gave 45,8 % higher of tobacco yield than the control by 1,084 ton ha-1 and 0,497 ton ha-1 respectively.

Comparison of Three DNA Isolation Methods of Aspergillus Niger Adyan Donastin; Maharani Pertiwi Koentjoro; Muhamad Taufik Hidayat; Endry Nugroho Prasetyo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p07

Polymerase Chain Reaction (PCR) is a technique that is applied to detect and test the presence of genetic material from pathogenic fungi. This method was further developed because it has a high sensitivity compared to the culture method in detecting the presence of pathogenic fungi. To perform sensitive, specific and reliable PCR-based assays, the availability of pure DNA as well as an easy-to-perform DNA extraction protocol is essential. Currently, existing protocols for DNA extraction generally require specialized kits and with the addition of enzymes. Therefore, this study was conducted to compare the quantity and quality of Aspergillus niger DNA isolation with three different methods. The stages of the research carried out included pure A. niger culture and total DNA isolation using three methods, namely the protocol according to the Wizard® Genomic DNA Purification Kit (P1) instructions, Modified Wizard® Genomic DNA Purification Kit (P2) and Monarch Genomic DNA Purification Kit NEB (P3). The results of the evaluation of DNA isolation using a spectrophotometer at a wavelength of A260/280 nm showed a ratio of 1.4, 1.8, and 1.7 at P1 and P2, and P3 respectively. The quantity obtained ranged from 210 to 305 ng/µL. The total DNA results obtained were then used for PCR testing of ITS ribosomal DNA fragments using ITS1 and ITS4 primers. Electrophorosis observation of PCR results showed that all samples produced bands with a length of 500 bp. The results of DNA isolation in the P1 method did not show bands on the agarose gel, but could be seen in the PCR product. Methods P2 and P3 showed DNA that had good quality and quality. The P2 and P3 methods use a cell destruction technique with liquid nitrogen and a combination of addition of proteinase K. The obtained protocol is expected to provide fast and good method information in the isolation of total DNA from A. niger. Keyword: Aspergillus niger, total DNA isolation, ITS1, ITS4

PENGARUH MASA SIMPAN DAN JENIS PENGIKAT GRAFTING TERHADAP KEBERHASILAN GRAFTING SIRSAK (Annona muricata) VARIETAS RATU Tri Wahyu Hidayat; Ali Mustafa Sidauruk; Rico Hutama Sulistiyo; Buana Susilo; Lengga Nurullah Dalimartha; Eko Chandra Wiguna; Isdiantoni Isdiantoni; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetyo
Biogenesis Vol 14, No 2 (2018)
Publisher : Program Studi Pendidikan Biologi

ABSTRACTIn nursery technology, the storage of scions always requires approriate optimization of shelf life and good packing This study aims to determine the effect of shelf life and storage materials on viability of soursop (Annona muricata) var. Ratu grafting by applying randomized factorial design with 2 factors: storage time and type of packing materials, with 3 times repeatation. The duration of storage time factor consist of 5 levels: 0 day, 3 days, 5 days, 1 week and 2 weeks meanwhile packing materials factor consist of 2 levels: parafilm and polyethylene. The highest survival rate was obtained at 0 day shelf life time and parafilm as storage material, whereas the lowest one was shown at 2 weeks storage with polyethylene as storage material.Keywords: Scion, Grafting, Storage, Packing Material, Soursop

PENAMBAHAN BIOCHAR BERBASIS LIMBAH PADA INCEPTISOL TERHADAP BUDIDAYA TEMBAKAU (Nicotiana tabacumL.) VARIETAS KEMLOKO DI TEMANGGUNG Katon Sasongko Damarmoyo; Buana Susilo; Lengga Nurullah Dalimartha; Eko Chandra Wiguna; Isdianto Isdianto; Maharani Pertiwi Koentjoro; Endry Nugroho Prasetiyo
Biogenesis Vol 14, No 2 (2018)
Publisher : Program Studi Pendidikan Biologi

ABSTRACTBiochar is a biological charcoal derived from incomplete combustion (pyrolysis) of agricultural residual materials that can increase carbon sustainably, retention of water and nutrients in soil. This research aimed to demonstrate the effect of adding biochar on inceptisol for Kemloko tobacco cultivation in Temanggung, Central Java, Indonesia. The data analysis of this research was using Factorial Random Design with two factors (treatment and dose). The results showed that the addition of biochar + compost increase productivity of 491.6 kg/ha comparing to the control with the higher tobacco quality. Chemical analysis of the respected soil showed that the biochar + compost treatment increase pH level up to 5.46 with the organic matter level and total nitrogen as well increased about 2.66% and 0.180% respectively.Keywords: biochar, tobacco of kemloko variety, temanggung, soil chemical

Local Perception and Behavior in Domestic Waste Management and Water Quality of The Brantas River, Dinoyo Ward Anggayasti, Wresti L.; Al Zamzami, Ilham Misbakudin; Pramudia, Zulkisam; Dwi Susanti, Yogita Ayu; Moehammad, Khibar Syiar; Ulfa, Siti Mariyah; Efani, Anton; Koentjoro, Maharani Pertiwi; Amin, Abd Aziz; Yanuar, Adi Tiya; Salamah, Lutfi Ni’matus; Galisong, Ridwan Danuarta; Aulia, Lisa; Kurniawan, Andi
Jurnal Pembangunan dan Alam Lestari Vol. 15 No. 2 (2024): Jurnal Pembangunan dan Alam Lestari
Publisher : Postgraduate School of Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2024.015.02.04

Waste management by society not only reflects individual responsibility but also plays a crucial role in environmental sustainability and community welfare. This research aimed to understand society’s perception and behavior regarding domestic waste and its surrounding waters. Understanding societal behavior is the key to sustainable environmental management. Direct inquiry was conducted to the sample population of 25 respondents who represented various age, education, and cultural groups in the densely populated residential area surrounding the Brantas River in Dinoyo Ward. Field observations were done to take water quality parameters and monitor the residents’ behavior regarding waste management. The results indicated that 76% of respondents know the impact of river pollution, although most did not contribute actively to reducing their domestic waste volume. About 44% of respondents never saw anyone littering the river, which may be due to the regular waste pick-up system. This was supported by water quality parameters that did not demonstrate any sign of pollution, although high turbidity was indicated. Comprehension of the results of this study is hoped to inspire the ideas of solid intervention plans to increase awareness and decrease the negative impact of domestic waste on the Brantas River in Dinoyo Ward. Keywords: society perception and behavior, domestic waste management, aquatic environment, Brantas River

Co-Authors Adyan Donastin Afifah, Kufah Nur Ahmad Nuril Fuad Al Fatih Al Fatih, Ahmad Nuril Fuad Al zamzami, Ilham Misbakudin Ali Mustafa Sidauruk Alviani, Melinda Nuril Amin, Abd Aziz Andi Kurniawan Anggayasti, Wresti L. Anggraeni, Ike Listya Anthon Efani Arief Rachmansyah Arifin, Nur Hikmah Ashari, Fadhil Muhamad Ayuningtyas Megawati Bambang Semedi Buana Susilo Bunga Hidayati Dalimartha, Lengga Nurullah Dwi Susanti, Yogita Ayu Efani, Anton Eko Chandra Wiguna Endry Nugroho Prasetiyo Endry Nugroho Prasetyo Eti Setiawati, Eti Fitriyah, Ikhrotul Galisong, Ridwan Danuarta Gunawan, Fahdynia Karnira Habibah, Laila Nur Harsuko Riniwati Herni Sudarwati Hidayat, Muhammad Taufiq Ida Ekawati Ina Irawati, Ina Isdianto Isdianto Isdiantoni Isdiantoni Isdiantoni Kartikaningsih, Hartati Katon Sasongko Damarmoyo Katon, Damarmoyo Sasongko Lengga Nurullah Dalimartha Lisa Aulia, Lisa Masruroh, Inayatul Moehammad, Khibar Syiar Mufidah, Elya Muhammad Fathur Rouf Hasan Muhammad Taufiq Hidayat Nurlelih, Euis Elih Pahlevi, Reza Widhi Pramudia, Zulkisam Prasetiyo, Endry Nugroho PUJI LESTARI Rico Hutama Sulistiyo Rini Yulianingsih Salamah, Lutfi Ni’matus Setyo Nugroho Siti Asmaul Mustaniroh Siti Mariyah Ulfa Sri Sudaryanti, Sri SURYADI, DEDE Susilo, Buana Tri Wahyu Hidayat Wati, Henny Diana Wiguna, Eko Chandra Wike, Wike Yanuar, Adi Tiya YOGA DWI JATMIKO Yusuf Efendi

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