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All Journal METAMORFOSA Journal of Biological Sciences INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Biopendix: Jurnal Biologi, Pendidikan dan Terapan Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia Prosiding Seminar Nasional Pengabdian Kepada Masyarakat
Muhammad Taufiq Hidayat
Department of Medical Laboratory Technology, FKes, Nahdlatul Ulama University of Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Nursing Public Health Social Sciences

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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument Muhammad Taufiq Hidayat; Endry Nugroho Prasetyo
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.1863

Abstract

RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.
Co-Authors Adyan Donastin Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Endah Prayekti Endry Nugroho Prasetyo Endry Nugroho Prasetyo Imratul Mukarramah Lili Susan Rahmawati Maharani Pertiwi Koentjoro Maharani Pertiwi Koentjoro Maki Zamzam Muhammad Afwan Romdloni Prayekti, Endah Romdhoni, Muhammad Afwan Sari, Devi Fitriana Setyo Nugroho Widya Novitasari