Garuda - Garba Rujukan Digital

Toksisitas Ekstrak Etanol Mangifera foetida L. sebagai Pengkelat Besi Ditinjau dari LD50 dan Komponen Sel Darah Wahyuni, Tri; Sari, Santi Purna; Estuningtyas, Ari; Freisleben, HJ
Pharmaceutical Sciences and Research Vol. 2, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Ethanolic extract of bacang mango leaves (Mangifera foetida L.) decreased iron concentration in blood Sprague dawley rats that had been induced iron overload. The aim of this experiment was to determine acute toxicity (LD50) value of ethanolic extract of M.foetida by weil method and its effect to blood component. This study was conducted by employing a complete random design using 25 male and 25 female mice of DDY strain which devided into 5 groups. The first group until fourth group were administered ethanolic extract of M.foetida with dose variation which suspension in aquadest orally. The fifth group was control that administered aquadest. The LD50 was determined by the total of death on all group. LD50 value of the extract showed no death in the biggest doses (13.013 g/kg). The examination was continued with measured blood count (erythrocites, trombocytes, leukocytes, and haemoglobin concentration). It was shown that the extract at dose 1.626; 3.253; 6.506 and 13.013 g/kg unchanged the blood count measurement.

Hematological Profile of Iron Overload in Rats Administered with Fruit Extract of Mahkota Dewa (Phaleria macrocarpa): Gambaran Hematologi Tikus Model Besi Berlebih yang Diberi Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) Fransisca Dela Verna; Ari Estuningtyas
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 8 No. 2 (2022): (October 2022)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2022.v8.i2.15936

Background: Repeated blood transfusions lead to an accumulation of iron that exceeds the body's iron storage capacity. Free iron is able to catalyze the formation of hydroxyl radicals that cause oxidative damage and cell death. The use of iron chelating medicines for iron chelation therapy is limited by side effects. Mangiferin is a natural bioactive compound with iron chelating and antioxidant activity. Mahkota dewa is a plant native to Indonesia that contains mangiferin. Objectives: The aim of this study was to evaluate the effect of mahkota dewa fruit extract administration on hematological profile of iron overload rats. Material and Methods: Thirty rats were divided into 6 groups: normal rats (N), iron overload rats (IO), iron overload rats rats given deferiprone (D), mangiferin (M), Mahkota dewa fruit extract at a dose of 100 mg/kg BW (PM1), or 200 mg/kg BW (PM2). Rats were given injection of iron sucrose 15 mg every 3-4 days for 8 weeks. At week 8, rats were sacrificed. Hematological analysis was performed in this study. Results: Iron overload condition caused by iron sucrose injection did not cause statistically significant changes in the haematological profile. Likewise, the administration mahkota dewa fruit extract at a dose of 100 or 200 mg/kg BW did not cause changes in the hematological profile. Conclusions: The administration of mahkota dewa fruit extract did not alter hematological profile of iron oveload rats.

The Effect of Ethanol Extract of Phaleria macrocarpa Fruit Combined with Deferiprone on Peripheral Blood Counts in Iron-Overloaded Rats Rahma, Rahma; Estuningtyas, Ari
Pharmaceutical Journal of Indonesia Vol. 10 No. 1 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2024.010.01.4

Iron overload poses health risks due to its role in reactive oxygen species (ROS) formation. We evaluated the efficacy of ethanol extract of Phaleria macrocarpa Fruit (PM) against deferiprone-induced alterations in hematological parameters in iron-overloaded rats. Six groups were studied: control, iron-overloaded (IO), deferiprone (D), PM, and two combination groups (DPM-1 and DPM-2). Hematological parameters were assessed at baseline (week-3) and post-treatment (week-8), including total white blood cell count (WBC), lymphocytes (LYM), granulocytes (GRAN), platelet count (PLT), red blood cell count (RBC), hemoglobin (Hb), mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC). Iron overload induced mild changes, with significant increases in MCV, alongside non-significant increasing trend in LYM and decreasing trends in other parameters. Deferiprone led to significant decreases in RBC and Hb, with non-significant increases in MCV and non-significant decreases in other parameters. PM group showed significant decreased in PLT, RBC, and Hb, and a significant increase in MCV and MCH, accompanied by non-significant increase in MCHC and non-significant decreasing trends in WBC, LYM, and GRAN. Combination treatment of ethanol extract of Phaleria macrocarpa fruit with deferiprone at usual dose (DPM-1) resulted in significant changes, including decreases in GRAN, RBC, Hb and MCHC and increasing MCV, accompanied by non-significant increase in MCH and non-significant decrease in other parameter. The magnitude of parameter changes appears smaller when the dose of deferiprone is reduced in the combination group (DPM-2) compared to the DPM-1 group. PM alone exhibited minimal effects on hematological parameters compared to deferiprone (except for PLT), indicating the need for further research to elucidate the specific cellular and molecular pathways influenced by these treatments to support the use of PM as adjunct therapy in patients with iron overload.

Modulation of membrane transporter expression and activity by mangiferin, epigallocatechin-3-gallate (EGCG), quercetin, and kaempferol: A review Nazihah, Maryam; Estuningtyas, Ari
Indonesian Journal of Pharmaceutical Science and Technology Vol 11, No 3 (2024)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v11i3.47068

Membrane transporters are one of the important factors in drug pharmacokinetics. These proteins mediate drug transport in and out of cells; efflux transporters export the drug from inside the cell, while influx transporters facilitate the entry of it into the cell. Thus, the presence of these membrane proteins influences drug levels in cells, which then determines the drug efficacy and safety profile. Many studies have shown that various exogenous compounds can modulate the activity and/or expression of membrane transporters, including bioactive molecules. Flavonoids are secondary plant metabolites that are very abundant and widely used in diets, supplement products, and traditional medicine. Apart from their medical benefits, flavonoids have been reported to interact with membrane transporters involved in drug absorption and drug resistance. These interactions can be beneficial in multidrug resistance conditions or awareness of drug toxicity. This article collects studies that have been conducted on four widely consumed plant-derived compounds: mangiferin, epigallocatechin-3-gallate (EGCG), quercetin, and kaempferol, and their effects on membrane transporters. The effects can be a consideration when administering these flavonoids together with medication.

The Effect of Ethanol Extract of Phaleria macrocarpa Fruit Combined with Deferiprone on Peripheral Blood Counts in Iron-Overloaded Rats Rahma, Rahma; Estuningtyas, Ari
Pharmaceutical Journal of Indonesia Vol. 10 No. 1 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2024.010.01.4

Iron overload poses health risks due to its role in reactive oxygen species (ROS) formation. We evaluated the efficacy of ethanol extract of Phaleria macrocarpa Fruit (PM) against deferiprone-induced alterations in hematological parameters in iron-overloaded rats. Six groups were studied: control, iron-overloaded (IO), deferiprone (D), PM, and two combination groups (DPM-1 and DPM-2). Hematological parameters were assessed at baseline (week-3) and post-treatment (week-8), including total white blood cell count (WBC), lymphocytes (LYM), granulocytes (GRAN), platelet count (PLT), red blood cell count (RBC), hemoglobin (Hb), mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC). Iron overload induced mild changes, with significant increases in MCV, alongside non-significant increasing trend in LYM and decreasing trends in other parameters. Deferiprone led to significant decreases in RBC and Hb, with non-significant increases in MCV and non-significant decreases in other parameters. PM group showed significant decreased in PLT, RBC, and Hb, and a significant increase in MCV and MCH, accompanied by non-significant increase in MCHC and non-significant decreasing trends in WBC, LYM, and GRAN. Combination treatment of ethanol extract of Phaleria macrocarpa fruit with deferiprone at usual dose (DPM-1) resulted in significant changes, including decreases in GRAN, RBC, Hb and MCHC and increasing MCV, accompanied by non-significant increase in MCH and non-significant decrease in other parameter. The magnitude of parameter changes appears smaller when the dose of deferiprone is reduced in the combination group (DPM-2) compared to the DPM-1 group. PM alone exhibited minimal effects on hematological parameters compared to deferiprone (except for PLT), indicating the need for further research to elucidate the specific cellular and molecular pathways influenced by these treatments to support the use of PM as adjunct therapy in patients with iron overload.

Molecular Docking of Active Compounds from The Ethanol Extract of Phaleria macrocarpa Fruit with Iron Transporters DMT1 and ZIP14 Rahma, Rahma; Estuningtyas, Ari
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 02 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss02/508

Iron-overload can lead to organ damage by promoting free radical production.This study explores the potential inhibitory effects of compounds found in Phaleria macrocarpa fruit on non-transferrin-bound iron uptake by targeting DMT1 and ZIP14 iron transporters through in-silico methods.The study utilized homology modeling to construct 3D structures of DMT1 and ZIP14. Validation of these models was carried out by assessing their sequence identity and analyzing their stereochemical quality using Ramachandran plots. Molecular docking was conducted using AutoDockTools. The coordinates for molecular docking were carefully chosen based on the iron binding-site locations as reported in previous literature. Interaction visualization was done using LigPlot+. Our findings indicate strong binding affinities of several compounds from Phaleria macrocarpa fruit with both DMT1 and ZIP14. Specifically, patuletin-7-O-[6"-(2-methylbutyryl)]-glucoside, naringenin-4'-7-dimethyl ether, and 5,7,8,3',4'-pentamethoxyflavone demonstrated significant interaction with DMT-1, while 6'-O-galloyl-homoarbutin, patuletin-7-O-[6"-(2-methylbutyryl)]-glucoside, and guanine exhibited high affinity for ZIP14. While the ethanol extract of Phaleria macrocarpa fruit shows promising interactions with key iron transporters implicated in iron overload, these in-silico predictions require further experimental validation to confirm their efficacy as inhibitors.

Iron-Overload Conditions: Manifestations to the Kidney Organs – A Review Heriatmo, Nadia Larasinta; Estuningtyas, Ari; Soetikno, Vivian
Borneo Journal of Pharmacy Vol. 6 No. 4 (2023): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v6i4.4411

Excess iron is a risk factor for organ dysfunction and damage resulting in various organ diseases such as liver, heart, and kidney, diabetes mellitus, and neurodegenerative diseases. Iron overload in some individuals is caused by various factors, including genetic predisposition such as genetic hemochromatosis, repeated transfusion of red blood cells, and parenteral iron administration in conditions of transfusion-dependent anemia. A disturbance in the globin gene in diseases such as β-thalassemia major causes an imbalance of the globin chain, resulting in chronic anemia in the sufferer. It has been reported that the human body does not have a mechanism for eliminating excess iron levels. Routine transfusion has become a solution to overcome chronic anemia so that patients can maintain hemoglobin levels, and the result of this transfusion repetition is the accumulation of iron in various organs, such as the heart, liver, endocrine glands, pancreas, lungs, and kidneys. Excess iron can be toxic to the body due to the formation of harmful free radicals that can damage cells and tissues. An increase in excessive ROS can result in the saturation of the antioxidant system. The presence of free radicals can lead to damage and the occurrence of filtration dysfunction in the glomerulus.

Title

Found 7 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 7 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 7 Documents
Search