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All Journal Acta Biochimica Indonesiana Pharmaceutical Sciences and Research (PSR)
HANS-JOACHIM FREISLEBEN
Medical Research Unit, Faculty of Medicine, University of Indonesia
Biochemistry, Genetics & Molecular Biology

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Archaeosomes and Tetraether Lipid Liposome Freisleben, Hans-Joachim
Majalah Ilmu Kefarmasian Vol. 9, No. 1
Publisher : UI Scholars Hub

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Abstract

Archaea have phytanyl ether lipids which is one of the characteristics that separates them from bacteria and eukaryotes. Some archaea have also unique membrane spanning tetrae ther lipids (T EL); in Sulfolobus and Ihermoplasma species these TELmake up the majority of total membrane lipids. Archaeal lipids are able to form stable liposomal structures, both from membrane factions, mainly the polar membrane fraction, i.e. archaeosomes, or from highly purified TEL (tetraether lipid liposomes). Liposomes of the main polar lipid (MPL) from thermoacidophilic archaeon Ihermoplasma acidophilum were thoroughly inevstigated. Archaeosomes and TEL liposomes exhibit extremely low proton permeability and high stability at low pH, which makes them suitable to delivery of therapeutics and vaccines via the gastro-inetestinal route. Liposomes from egg lecithin can be stabilized by incorporation of 11-12 mole% MPL. MPL liposomes show long-term shelf stability even at high temperatures without conservation. The size of MPL liposomes between 100 nm (unilamellar) and several gm (multilamellar) depends mainly on the method of preparation. Toxicity and mutagenicity have not been detected in toxicological screening. This is a review report on the lipid from archaea to be developed as an alternative to conventional liposome for various industrial applications, especially for pharmaceutical industry.
Efficacy of liposomal amphotericin B eye drops at two concentrations in experimental aspergillus keratomycosis in rabbits Amalia, Husnun; Ridwan, Rahmawati; Estuningtyas, Ari; Sitompul, Ratna; Adawiyah, Robiatul; Freisleben, Hans-Joachim
Acta Biochimica Indonesiana Vol. 9 No. 1 (2026): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.240

Abstract

Background: Keratomycosis, a fungal corneal infection prevalent in tropical regions, frequently leads to visual impairment and blindness. Liposomal amphotericin B (L-AmB) offers improved efficacy and reduced toxicity compared to conventional amphotericin B deoxycholate and represents a promising option for topical ophthalmic use. Objective: To evaluate and compare the therapeutic efficacy of L-AmB eye drops at 0.15% and 0.5% concentrations in experimental Aspergillus keratomycosis in New Zealand White rabbits. Methods: Four rabbits received midstromal corneal inoculation with a mixed suspension of Aspergillus flavus, A. fumigatus, A. niger, and A. terreus (2 x 10⁴ CFU/0.05 mL). L-AmB eye drops were applied hourly from day 8 post-inoculation until corneal cultures became negative. Antifungal susceptibility was assessed by disc diffusion. Clinical response was monitored by slit lamp examination using a modified scoring system. Results: Negative corneal cultures were achieved in all treated eyes within 10 to 18 days (mean 15 days, L-AmB 0.15%) and 11 to 15 days (mean 14 days, L-AmB 0.5%). No toxic effects were observed. Complete corneal transparency without cicatrix was achieved in one eye treated with L-AmB 0.15%. Conclusion: Both concentrations were effective and safe. Liposomal AmB 0.15% appears as efficacious as 0.5% for experimental Aspergillus keratomycosis. Clinical studies in humans are warranted.
Co-Authors A.A. Ketut Agung Cahyawan W Alida Harahap Ani R. Prijanti Antonopoulos, Emmanouil Ari Estuningtyas, Ari Bethy S. Hernowo Budi Kurniawan Bundschu, Hans-Dieter David Tandian Fajri, Purnama Frans Ferdinal Freisleben, Seruni U. Haier, Jörg Husnun Amalia Isnani A.S. Suryono Jenny Hidayat Krista Kuebel-Thiel Melva Louisa Mohamad Sadikin Mohammad A. Aulia Na P. Bo Nia Yuliatri Oertl, Anton Pustika A. Wahidiyat Rahmawati Ridwan Ratna Sitompul Renindra A. Aman Robiatul Adawiyah Rondang R. Soegianto Rostika Flora Schäfers, Jürgen Septelia I. Wanandi Seruni K.U. Freisleben Soehardjo Poertadji Sri Handayani Syaiful Ichwan Tiwuk Susantiningsih