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Inhibitory Test Of Citronella Essential Oil (Cymbopogon Nardus L. Rendle) Againts Aspergillus Flavus Growth: INHIBITORY TEST OF CITRONELLA ESSENTIAL OIL Rahmat Pannyiwi; Menik Kasiyati; Martha Atik Martiningsih; Siti Nur Syariah; Suyana Suyana; Rita Rena Pudyastuti; Sujono Sujono
INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD) Vol. 5 No. 1 (2023): INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT
Publisher : STIKes Mandala Waluya Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36566/ijhsrd/Vol5.Iss1/143

Background: Indonesia is the one of tropical climate country with high air humidity. It becomes the main factor of fungal infection, that is called otomycosis, which is caused by Aspergillus flavus. The treatment of otomycosis is usually done by giving an azole antifungal drugs that can give the beneficial and adverse effects. Treatment of otomycosis can be done with various kinds of herbs. One of herbs that is used, it is citronella. Citronella contains essential oil 0,4% and in citronella essential oil contains citronellal 32-45% and geraniol 12-15% has ability as an antifungal. The aim of this study is to determine the inhibitory of citronella essential oil against Aspergillus flavus growth. Methods: The type of this research is laboratory experiments with posttest only with control group design. 24-hour Aspergillus flavus is used as the research subjects which had been inoculated on SDA media and given essential oils in various concentrations of 0.5%, 1.0%, 1.5%, 2.0 %. The observation of citronella essential oil inhibitory as an antifungal by measuring the diameter of inhibitory zone, it uses a caliper. Results: The measuring from average diameter of Aspergillus flavus fungal growth zone in each concentration, they are 0.5% ;1.0%; 1.5%; 2.0% is 9.11 mm; 12.03 mm; 13.62 mm; 16.81 mm. The higher concentration of essential oil, it makes the wider diameter of the inhibition zone. The level of sensitivity is intermediate, sensitive, sensitive, sensitive. The level of effectiveness is less effective, effective enough, effective, very effective. The results of statistical analysis showed that citronella essential oil has the inhibitory against Aspergillus flavus growth. Conclusion: this study was various concentration of citronella essential oil have a significanteffect on the growth of inhibitory Aspergillus flavus.

Inhibitory Test Of Citronella Essential Oil (Cymbopogon Nardus L. Rendle) Againts Aspergillus Flavus Growth: INHIBITORY TEST OF CITRONELLA ESSENTIAL OIL Rahmat Pannyiwi; Menik Kasiyati; Martha Atik Martiningsih; Siti Nur Syariah; Suyana Suyana; Rita Rena Pudyastuti; Sujono Sujono
INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT (IJHSRD) Vol. 5 No. 1 (2023): INDONESIAN JOURNAL OF HEALTH SCIENCES RESEARCH AND DEVELOPMENT
Publisher : STIKes Mandala Waluya Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36566/ijhsrd/Vol5.Iss1/143

Background: Indonesia is the one of tropical climate country with high air humidity. It becomes the main factor of fungal infection, that is called otomycosis, which is caused by Aspergillus flavus. The treatment of otomycosis is usually done by giving an azole antifungal drugs that can give the beneficial and adverse effects. Treatment of otomycosis can be done with various kinds of herbs. One of herbs that is used, it is citronella. Citronella contains essential oil 0,4% and in citronella essential oil contains citronellal 32-45% and geraniol 12-15% has ability as an antifungal. The aim of this study is to determine the inhibitory of citronella essential oil against Aspergillus flavus growth. Methods: The type of this research is laboratory experiments with posttest only with control group design. 24-hour Aspergillus flavus is used as the research subjects which had been inoculated on SDA media and given essential oils in various concentrations of 0.5%, 1.0%, 1.5%, 2.0 %. The observation of citronella essential oil inhibitory as an antifungal by measuring the diameter of inhibitory zone, it uses a caliper. Results: The measuring from average diameter of Aspergillus flavus fungal growth zone in each concentration, they are 0.5% ;1.0%; 1.5%; 2.0% is 9.11 mm; 12.03 mm; 13.62 mm; 16.81 mm. The higher concentration of essential oil, it makes the wider diameter of the inhibition zone. The level of sensitivity is intermediate, sensitive, sensitive, sensitive. The level of effectiveness is less effective, effective enough, effective, very effective. The results of statistical analysis showed that citronella essential oil has the inhibitory against Aspergillus flavus growth. Conclusion: this study was various concentration of citronella essential oil have a significanteffect on the growth of inhibitory Aspergillus flavus.

Increasing Women's Awareness and Concern for Breast Cancer through Health Education: Impact of Educational Programs on Early Detection and Prevention Rita Rena Pudyastuti; Sujono Sujono; Kariyadi Kariyadi; Johanna Tomasoa
Oshada Vol. 2 No. 1 (2025): Oshada Journal - February
Publisher : PT. Anagata Sembagi Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62872/jake1725

Breast cancer is one of the leading causes of death in women worldwide, with incidence rates increasing every year. Early detection and prevention are important steps in reducing mortality from this disease. Women's low awareness and understanding of breast cancer is a major barrier to early detection. This literature study aims to analyze the impact of health education programs on increasing women's awareness and concern about breast cancer, especially in the aspects of early detection and prevention. Based on the literature review, it was found that health education programs have a significant role in improving women's understanding of risk factors, early symptoms, and the importance of breast self-examination and clinical examination. A number of studies have shown that before receiving education, many women did not understand the importance of early detection and tended to neglect breast examination. After participating in the education program, there was an increase in knowledge and a positive change in attitude towards early detection, with more women willing to perform regular breast examinations. This study also revealed that interactive and community-based education methods are more effective than passive delivery of information. Thus, the results of this literature study confirm that health education is an effective strategy in increasing women's awareness and concern about breast cancer. The implementation of more innovative and technology-based education programs can be a solution in reaching more women, especially in areas with limited access to health services.

PENGGUNAAN OVEN SEBAGAI ALTERNATIF PEMANASAN PADA PROSES PEMATANGAN JARINGAN TERHADAP KUALITAS HISTOMORFOLOGI APPENDIX DENGAN PEWARNAAN HE (HEMATOXYLIN-EOSIN) UMI RAHMAWATI NINGRUM; ULLYA RAHMAWATI; SUJONO SUJONO; SITI NURYANI
Journal of Nursing and Public Health Vol 13 No 1 (2025)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37676/jnph.v13i1.8521

Pendahuluan: Proses pematangan jaringan meliputi dehidrasi, clearing dan infiltrasi paraffin. Proses pematangan jaringan yang dilakukan menggunakan Automatic tissue processor memudahkan tenaga laboratorium dalam memproses jaringan tetapi juga memerlukan waktu yang cukup lama. Suhu merupakan salah satu faktor yang dapat mempengaruhi tingkat pematangan jaringan. Suhu berpengaruh di dalam pelebaran celah membrane sel yang berdampak terhadap meningkatkan laju penetrasi dan pertukaran cairan. Penggunaan oven menjadi alternatif pemanasan pada proses pematangan jaringan agar proses menjadi lebih cepat. Tujuan dari penelitian ini untuk mengtahui bahwa oven dapat dijadikan sebagai alternatif pemanasan pada proses pematangan jaringan terhadap kualitas histomorfologi Appendix dengan pewarnaan HE (Hematoxylin-Eosin). Metode: Jenis penelitian ini menggunakan metode observasional dengan desain penelitian post-test only control group yaitu 16 sampel jaringan Appendix dilakukan proses pematangan jaringan menggunakan alat oven sebagai alternatif pemanasan selama 6 jam dan menggunakan Automatic tissue processor selama 16,5 jam sebagai control lalu diamati histomorfologi dari setiap perlakuan. Data dianalisis secara deskriptif menggunakan gambar dan grafik Hasil dan Pembahasan: Hasil penelitian proses pematangan jaringan Appendix menggunakan Automatic tissue processor selama 16,5 jam dan oven suhu 60OC selama 6 jam menghasilkan gambaran yang hampir sama. Kesimpulan: Oven dengan suhu 60OC selama 6 jam dapat digunakan sebagai alternatif pada proses pematangan jaringan terhadap kualitas histomorfologi Appendix dengan pewarnaan HE (Hematoxylin Eosin).

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