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Isolasi, Identifikasi Dan Uji Bioaktivitas Metabolit Sekunder Dari Fraksi n-Heksana Kayu Akar Tumbuhan Paliasa (Kleinhovia hospita Linn.) Yang Tidak Aktif Terhadap Udang Artemia salina Leach Jamius B Stepanus; Nunuk H Seokamto
Akta Kimia Indonesia Vol 8, No 1 (2023)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v8i1.16503

Abstract

Penelusuran secara etnobotani dan berdasarkan kajian kemotaksonomi serta filogenetik menunjukkan kayu akar tumbuhan Kleinhovia hospita Linn. (Paliasa) berpotensi menghasilkan metabolit sekunder dengan stuktur beragam dan bersifat bioaktif. Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi dan menentukan bioaktivitas metabolit sekunder pada fraksi n-heksan yang tidak aktif terhadap udang Artemia salina Leach dari kayu akar tumbuhan K. hospita. Metode isolasi yang digunakan terdiri dari ekstraksi, fraksinasi dan pemurnian. Identifikasi metabolit sekunder melalui analisis spektroskopi IR sedang uji bioaktivitas senyawa berdasarkan metode Brine Shrimp Lethality Test (BST) menggunakan A. salina. Hasil penelitian diperoleh dua senyawa yang diduga sebagai steroid alkaloidal dengan nilai toksisitas (LC50) masing-masing senyawa adalah > 1000 µg/mL dan 107,54 µg/mL. Kata kunci: Artemia salina Leach., Kleinhovia hospita Linn., spektrum IR, Sterculiaceae, steroid alkaloidal.
Energy auditing and electricity saving opportunities in BPOM laboratory of manokwari Adelhard Rehiara; Asril Yanto Musa; Jamius Bin Stepanus
Social, Ecology, Economy for Sustainable Development Goals Journal Vol. 1 No. 1: (July) 2023
Publisher : Institute for Advanced Science Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/seesdgj.v1i1.2023.22

Abstract

Energy auditing is a method of increasing energy efficiency. An energy audit is conducted to provide an overview of energy use, analyze the electrical system, and determine potential cost savings. The laboratory of the national food and drug agency (BPOM) in Manokwari has problems with electricity that often cause interruptions, power outages, and expensive bills. Therefore, this research was conducted to audit the energy used in the laboratory of the BPOM of Manokwari, with a building area of 1,484 m2 and an installed power of 105 kVA. In this laboratory building, a diesel generator with capacity of 5 kVA is installed as a backup energy source and the generator has its own installation line. According to the calculation results before auditing, the energy consumption intensity (IKE) value obtained per month is 10.18 kWh. Energy audit through lighting loads and air conditioning systems. Then the recommendation through energy savings for lights and air conditioners is 274.12 kWh/month and 639.32 kWh/month, respectively. Therefore, total savings are 913.44 kWh/month, or about IDR 1,541,886.72/month.
Exploration of Lipid Content in Sargassum binderi Algae from the North Coast of Manokwari West Papua Stepanus, Jamius Bin; Kolibongso, Duaitd
Indonesian Journal of Chemical Science and Technology (IJCST) Vol 7, No 1 (2024): JANUARY 2024
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ijcst.v7i1.56442

Abstract

The aim of this research is to determine the potential of biodiesel from the algae Sargassum binderi from the North Coast of Manokwari by determining the lipid components contained in the algae. Lipid extraction was carried out using the soxhletation method with n-hexane solvent. Lipid percentage was determined via GCMS analysis. The research results obtained 4 lipid components consisting of hexadecanoic acid (palmitic acid), 9,12-hexadecadienoic acid, 9-octadecenoic acid (Z) (oleic acid) and octadecanoic acid (stearic acid) with a percentage of 18.12%, 4.75%, 19.4% and 4.29% respectively. The total percentage of lipid components extracted was 46.56%, almost half of the total extract.
Preliminary Study on the Potential of Red Fruit Pigment (Pandanus conoideus) from West Papua as Dye-Sensitized Solar Cell (DSSC) Stepanus, Jamius Bin; Patiran, Abdul Zaid; Sumarna, Sabir; Islam, Muh. Fajar
Hydrogen: Jurnal Kependidikan Kimia Vol 12, No 6 (2024): December 2024
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v12i6.13441

Abstract

The red fruit (Pandanus conoideus) is an endemic plant from Papua, known for its distinctive color and shape. This fruit is recognized for its bioactive properties, such as antioxidant, anti-inflammatory, and antihyperglycemic effects. Its high pigment content is believed to have potential as a sensitizer in DSSC applications. However, research on this topic remains underexplored. Therefore, the aim of this preliminary study is to investigate the potential of red fruit pigments for DSSC. The characterization of red fruit pigments was conducted through phytochemical screening, FTIR and UV-Vis spectral analysis, as well as literature reviews. Pigment extraction was carried out using maceration without involving drying or grinding processes. Phytochemical screening results revealed that the macerate contains flavonoids, alkaloids, phenolics and terpenoids, compounds commonly used as natural pigments in DSSCs. FTIR analysis showed the presence of functional groups such as carboxyl (-COOH), carbonyl (C=O), and hydroxyl (-OH), which can act as effective anchoring groups when interacting with nanosemiconductor surfaces. Meanwhile, UV-Vis analysis showed absorption peaks in the UV region (wavelength 204–399 nm) and the visible region (wavelength 400–550 nm). Based on literature studies and research findings, it can be concluded that the pigments in red fruit have potential applications as DSSC sensitizers.
PRELIMINARY STUDY OF LIPID CONTENT IN BROWN ALGAE (Sargassum binderi) FROM MANOKWARI WATERS Stepanus, Jamius Bin
Molluca Journal of Chemistry Education (MJoCE) Vol 15 No 1 (2025): MJoCe
Publisher : Program Studi Pendidikan Kimia, FKIP, Universitas Pattimura (Chemistry Education Study Program, Faculty of Teacher Training and Educational Sciences, Pattimura University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol15iss1pp1-11

Abstract

Algae are marine plants that have the potential as raw material for producing biofuel (biodiesel, bioethanol and biogas) apart from being used as biomass. The high lipid content in algae can be converted into biofuel through esterification and transesterification chemical reactions. Research on the chemical composition of algae species found in West Papua remains limited. One type of algae commonly found in the waters of West Papua, especially on the northern coast of Manokwari is Sargassum binderi. Therefore, the aim of this study is to identify the types and percentages of lipid compounds contained in S. binderi. Lipid compounds were extracted using 2 different extraction methods, the maceration method and the Bligh Dyer method. The extraction process uses wet samples of S. binderi without drying, grinding or heating treatments. The type and percentage of lipids were determined via GC-MS analysis. The study results revealed six lipid compounds obtained from the maceration method: tetradecanoic acid (1.4%), 10-octadecenoic acid (4.69%), hexadecanoic acid (19.15%), 9-octadecenoic acid (Z) (5.93%), octadecanoic acid (2.71%), and 5,8,11,14-eicosatetraenoic acid (1.01%), with a total lipid compound percentage of 34.89%. Meanwhile, from the Bligh Dyer method, five lipid compounds were identified: 9-octadecenoic acid (Z) (4.33%), hexadecanoic acid (46.07%), 9,12-hexadecadienoic acid (5.23%), 11-octadecenoic acid (27.18%), and 5,8,11,14-eicosatetraenoic acid (2.81%), with a relatively high total lipid percentage of 85.62%. Hexadecanoic acid, also known as palmitic acid, was the lipid compound with the highest percentage found in both extraction methods.
Realisasi Program Pengabdian KKN di Daerah Perdesaan Bagian Pedalaman Papua Barat Stepanus, Jamius Bin; Momo, Kristian Ronaldo; Nabila, Adinri Rizki; Ratte, Yansi; Padang, Olivia Sau; Hutasoit, Sugi Tiber; Fertikasari, Julia Cindy; Worengga, Frengky V.L.P.; Mayor, Oktofina; Komariah, Intan; Jitmau, Martina M.; Gobai, Isayas
Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2025): Edisi Mei 2025
Publisher : FKIP Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36709/amalilmiah.v6i2.371

Abstract

Pelaksanaan program pengabdian Kuliah Kerja Nyata (KKN) idealnya memilih lokasi daerah perdesaan. Hal ini berdasarkan pertimbangan bahwa kondisi daerah perdesaan pada umumnya memiliki akses pendidikan yang terbatas, tingkat pelayanan kesehatan yang masih rendah serta infrastruktur yang belum memadai. Tujuan dari kegiatan ini adalah untuk merealisasikan program-program pengabdian yang relevan dengan kebutuhan masyarakat di daerah perdesaan wilayah Papua Barat sekaligus menumbuhkan semangat pengabdian dalam diri mahasiswa. Alur kegiatan KKN terdiri dari 6  tahapan yaitu (i) pembekalan; (ii) wawancara dan survei lapangan; (iii) perencanaan program kerja; (iv) pelaksanaan; (v) monev; (vi) seminar dan penyusunan laporan. Anggota kelompok KKN Kampung Aska terdiri dari 11 mahasiswa (dari 10 program studi) yang dibimbing oleh 1 dosen pembimbing lapangan. Total 50 program kerja (pk) dari 9 bidang utama berhasil dijalankan yang terdiri dari bidang pendidikan (6 pk), lingkungan (9 pk),  administrasi kampung (8 pk), kesehatan (5 pk), digitalisasi lokasi kampung (2 pk), keagamaan (4 pk), pertanian (9 pk),  peternakan (1 pk) dan ekonomi sosial budaya (6 pk). Hasil kegiatan pengabdian antara lain meningkatnya kemampuan literasi dan numerik siswa, lingkungan kampung menjadi bersih, tersedianya papan-papan administrasi kampung, warga mendapatkan edukasi tentang bencana alam dan mitigasi, stunting, DBD serta malaria.
Penentuan Hubungan Matematis antara Berat Rendemen dan Volume Maserat Menggunakan Regresi Linear Sederhana sebagai Solusi Alternatif Saat Alat Rotary Evaporator Mengalami Kerusakan Stepanus, Jamius Bin
Jurnal Penelitian Sains Vol 27, No 2 (2025)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v27i2.1177

Abstract

Penelitian ini bertujuan untuk mempelajari hubungan matematis antara berat rendemen ekstrak dan volume maserat. Persamaan matematis ini berguna untuk memprediksi berat rendemen (variabel dependen) dari data volume maserat (variabel independen) ketika alat rotary evaporator laboratorium tidak dapat digunakan karena mengalami kerusakan. Metode analisis yang digunakan dalam penelitian ini adalah regresi linear sederhana untuk menentukan model         persamaan regresi yang menyatakan korelasi antar kedua variabel, menghitung variansi galat acak, menguji hipotesis (uji model adequacy, uji F dan uji intercept) serta menghitung koefisien determinasi adjusted. Berdasarkan penelitian terhadap sampel uji kulit buah matoa, diperoleh model persamaan regresi y = 0,0200x  dengan variansi galat acak, s2 = 0,000160 dan koefisien determinasi adjusted, Ra2 = 96,12%. Sehingga untuk 250 mL volume maserat kulit buah matoa, diprediksi memiliki berat rendemen ekstrak sebesar 4,94 g.
Ekstraksi Komponen Lipid dari Mikroalga Nannochloropsis oculata dan Nitzschia sp. dengan Metode Sokletasi dan Bligh Dyer: Extraction of Lipid Components from Nannochloropsis oculata and Nitzschia sp. Microalgae with Soxhletation and Bligh Dyer Methods Stepanus, Jamius Bin
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 3 (2023): December Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i3.16596

Abstract

Dependence on fossil energy sources as non-renewable energy will trigger the threat of an energy crisis in Indonesia in the future. The solution to this issue is through the development of renewable energy and one of them is through the exploration and utilization of biomass such as microalgae. Microalgae have a high lipid content (4-77%) and have potential as raw materials for producing biofuel (biodiesel, bioethanol, and biogas). Lipids can be converted into biodiesel through acid or base-catalyzed esterification and transesterification reactions. The aim of this research was to determine the percentage of lipid components, namely free fatty acids (FFA), monoglycerides (MAG), diglycerides (DAG), and triglycerides (TAG) from microalgae extracts of Nannochloropsis oculata and Nitzschia sp. using Soxhletation and Bligh Dyer extraction methods. This research also aims to determine the comparison of lipid yields from the two extraction methods. Lipid yield percentage was determined through gas chromatography analysis. The research results showed that the percentages of FFA, MAG, DAG, and TAG for N. oculata ranged from 3.34 - 33.79% (soxhletation) and 0 - 17.54% (bligh dyer). Meanwhile, for Nitzschia sp., the percentage ranged from 7.16 - 26.28% (soxhletation) and 0.45 - 13.73% (bligh dyer). Lipid yield for N. oculata was 1.81% (soxhletation) and 2.21% (bligh dyer), while for Nitzschia sp., 0.96% (soxhletation) and 2.10% (bligh dyer). Based on the results of both extraction methods, it was found that the total lipid percentage of the microalgae N. oculata was higher than Nitzschia sp.
Analysis of Lipid Yield from Chaetoceros calcitrans Microalgae Extract Using Gas Chromatography Stepanus, Jamius Bin; Likumahwa, Fervin Mayos; Kesauliyaa, Olivia Marie Caesaria; Angrianto, Novaldi Laudi; Sumarna, Sabir; Islam, Muh. Fajar
Jurnal Kimia Fullerene Vol 10 No 2 (2025): Fullerene Journal Of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v10i2.724

Abstract

Renewable energy (RE) is a solution to the threat of the upcoming energy crisis. The increasingly massive energy transition is expected to significantly reduce the use of fossil fuels. Microalgae are one of the potential sources of RE for bioenergy (biodiesel) production due to their lipid content. This research was conducted to determine the percentage of lipid components; free fatty acids (FFA), monoglycerides (MAG), diglycerides (DAG), and triglycerides (TAG) in Chaetoceros calcitrans microalgae and to compare lipid yields using two extraction methods: Bligh-Dyer (wet samples) and Soxhlet extraction (dry samples).The results for C. calcitrans extracted using the Bligh-Dyer method showed that the percentage of lipid components (FFA, MAG, DAG, and TAG) ranged from 5.44% to 17.10%, with a total of 47.92%, and the lipid yield from 6 g of wet sample was 5.07%.Meanwhile, for the C. calcitrans extract using Soxhlet method, it was found that the percentage of lipid components (FFA, MAG, DAG and TAG) ranged from 6.79 to 18.01% with a total of 63.08% and the lipid yield from 20 g of dry samples was 0. 44%.