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All Journal Biosfer: Jurnal Tadris Biologi Jurnal Penelitian Sains Bioscientist : Jurnal Ilmiah Biologi Sriwijaya Bioscientia BIOVALENTIA: Biological Research Journal

Singgih Tri Wardana

Jurusan Biologi, FMIPA Universitas Sriwijaya, Sumatera Selatan

Author-ID : 624701

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Articles

MORPHOLOGICAL CHARACTERISTICS OF NEPENTHES IN PEAT SWAMP AREA OF TULUNG SELAPAN, SOUTH SUMATERA Wardana, Singgih Tri; Ilmawati, Ika; Tanzerina, Nina; Juswardi, Juswardi; Aminasih, Nita; Harmida, Harmida
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

This study is aimed to recognise the morphological characteristics of Nepenthes mirabilis and Nepenthes gracilis in peat swamp area including stem, leaf, tendril, pitcher, flower, fruit, and seed.Â This study has been conducted in JanuaryÂ until April 2018, located in Petaling Village Tulung Selapan Sub-District, Ogan Komering Ilir, South Sumatera. This study uses an exploratory method and the the observation sample using the method of random plot is chosen by purposive sampling. Parameters observed morphological characteristics of Nepenthes include stems, leaves, tendrils, pitcher, flower, adn fruit, as well as measurement of environmental factors. Based on the study, the researcher obtained two types of Nepenthes which are Nepenthes mirabilis and Nepenthes gracilis inÂ peat swamp area of Petaling Village Tulung Selapan Sub-District, Ogan Komering Ilir. Nepenthes mirabilis has a teres stem, leaves that in the form of oblong to lanceolate, has a petiolate, the edge of the leaves is flat covered by smoothy hair, thick peristome in size of 0,4-0,5 cm. Meanwhile stem Nepenthes gracilis has triangular stem, leaves in formed of lanceolate, sessile, flat edge of leaves, thin peristome in size of 0,1-0,2 cm. The pitcher shape of Nepenthes mirabilis and Nepenthes gracilis are cylindrical, infundibulate, and waist. The flower of Nepenthes mirabilis and Nepenthes gracilis are compound in type ofÂ raceme inflorescences. The fruit is formed as capsula, has four lobed, and contains numerous seeds. The seed is spindle-like and white colored.

Variasi Rasio Amonium dan Nitrat terhadap Perkembangan Embrio Somatik Bawang Putih (Allium sativum) secara In Vitro Singgih Tri Wardana
Jurnal Penelitian Sains Vol 18, No 2 (2016)
Publisher : Faculty of Mathtmatics and Natural Sciences

This research was aimed to conduct different NH4 + : NO3 -ratios on the development of somatic embryos of garlic (Allium sativum) by in vitro technique. This research used complete random design with four levels of NH4 + : NO3 -ratios, i.e. 1:2, 1:3, 1:4, and 1:5.The cell suspension culture used MS medium with 0,25 mg l-1 BAP. The results showed that the different NH4 + : NO3 -ratios resulted significant effect on the development of somatic embryos ( globular, scutelar, and coleoptiler stages).1:3 ratio of NH4 + : NO3 -was optimum to improve the development of somatic embryos.

Induksi Kalus Embriogenik Bawang Putih (Allium Sativum L.) dengan Rasio Amonium dan Nitrat yang Bervariasi Singgih Tri Wardana
Jurnal Penelitian Sains No 16 (2004)
Publisher : Faculty of Mathtmatics and Natural Sciences

Penelitian tentang pengaruh rasio amonium (NH4) dan nitrat (NO3) yang bervariasi terhadap induksi kalus embriogenik secara in vitro pada eksplan umbi bawang putih (Allium sativum L.) telah dilakukan. Penelitian ini bertujuan untuk mendapatkan rasio NH4 : NO3 yang baik untuk induksi kalus embriogenik bawang putih. Rancangan acak lengkap (RAL) digunakan dalam penelitian ini, dengan perlakuan rasio NH4 : NO3 yang bervariasi yaitu 1:2, 1:3, 1:4 dan 1:5. Hasil penelitian menunjukkan bahwa rasio NH4 : NO3 yang bervariasi berpengaruh nyata pada presentasi kalus embriogenik dan kalus yang friabel. Presentasi tertinggi untuk kalus embriogenik dan kalus friabel adalah rasio NH4 : NO3 = 1:3. Kalus embriogenik cenderung berwarna kekuningan dan bertekstur friabel serta perkembangan embrio somatik yang terbentuk pada stadium globuler.

Induksi Kalus Embriogenetik Bawang Putih (Allium Sativum L,) Secara In Vitro Singgih Tri Wardana
Jurnal Penelitian Sains No 15 (2004)
Publisher : Faculty of Mathtmatics and Natural Sciences

Penelitian tentang induksi kalus embriogenik bawang putih (Allium sativum L.) secara in vitro telah dilakukan. Hasil yang diperoleh bahwa jenis auksin (2,4-D dan NAA) dengan konsentrasi yang berbeda (0,5 dan 1,5 mg/l) berpengaruh nyata terhadap induksi kalus embriogenetik dan kalus friabel. Jenis auksin 2,4-D dan NAA masing-masing dengan konsentrasi 1,5 mg/l merupakan yang terbaik dalam menginduksi kalus embriogenetik dan kalus friabel. Kalus embriogenetik yang terbentuk bertekstur friabel dan berwarna kuning, serta perkembangan embrio somatik pada stadium globuler.

Respons Pertumbuhan Rimpang Bangle (Zingiber purpureum) Pada Perlakuan PGPR (Plant Growth Promoting Rhizobacteria) Wardana, Singgih Tri; Mangandi, Wulan Fransisca; Harmida, Harmida; Setiawan, Doni
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13142

Bangle (Zingiber purpureum Rosc.) is one of the species of the Zingiberaceae family that has medicinal properties. Secondary metabolites contained in bangle rhizomes have biological activity as antioxidants, anti-inflammatory, anticancer, anti-asthma, anti-aging, neuroprotective, antimicrobial. Environmentally friendly technological innovation to increase soil nutrient content and plant growth in sustainable plant cultivation efforts is by utilizing plant growth-promoting rhizobacteria (PGPR). This study aims to determine the effect of PGPR concentration on the growth of bangle rhizomes. This study was conducted at the Physiology and Development Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University. A completely randomized study design was used with five PGPR concentration treatments (0; 75; 125; 175; and 225 ppm). Data were analyzed by Analysis of Variance (Anova) and Duncan's Multiple Range Test (DMRT). The study showed that PGPR treatment can increase the growth of bangle rhizomes compared to no PGPR treatment. The best results at a PGPR concentration of 175 ppm in increasing the growth of bangle rhizomes.

Efektivitas auksin eksogen terhadap pertumbuhan tunas dan akar stek melati (Jasminum sambac (L.) Aiton) Wardana, Singgih Tri; Setiawan, Heri; Juswardi
Sriwijaya Bioscientia Vol 5 No 2 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/sribios.5.2.2024.490

Perbanyakan tanaman melati dapat dilakukan dengan cara vegetatif dan generatif, namun perbanayakan secara generatif sangat jarang dilakukan, karena bagian yang dimanfaatkan dari tanaman melati adalah bagian bunga. Secara umum perbanyakan melati dilakukan secara vegetatif, yaitu dengan cara stek. Salah satu faktor yang memengaruhi keberhasilan stek yaitu pemberian zat pengatur tumbuh auksin. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi auksin eksogen terhadap pertumbuhan stek melati (Jasminum sambac). Rancangan penelitian menggunakan Rancangan Acak Lengkap dengan perlakuan konsentrasi auksin eksogen. Konsentrasi auksin terdiri dari 0 mg/L, 75 mg/L, 150 mg/L, 225 mg/L dan 300 mg/L, masing-masing dengan 5 kali pengulangan. Data dianalisis menggunakan Anova taraf 5 %, jika berpengaruh nyata dilakukan uji Duncan (DMRT) taraf 5 %. Hasil penelitian menunjukkan auksin eksogen efektif dalam meningkatkan pertumbuhan tunas stek melati, tetapi tidak memengaruhi pertumbuhan akar stek melati.

DNA Barcoding Using the Chloroplast Gene MatK for Genetic Diversity Analysis of Lansium domesticum Corr. Plants Growing in South Sumatra, Indonesia Wardana, Singgih Tri; Hanum, Laila; Nursela, Dewi; Aminasih, Nita; Kasiamdari, Rina Sri
Biosfer: Jurnal Tadris Biologi Vol 16 No 1 (2025): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/bk0zrs84

Lansium domesticum Corr. known as duku, langsat, and kokosan is a popular fruit in Indonesia, particularly in South Sumatra. Furthermore, the fruit is cherished in this region because of the nice smell, sweet taste, and high economic value. Until now, South Sumatra local duku is rarely investigated, even though information on the genetic diversity of the germplasm is useful in supporting breeding and conservation programs. Therefore, this study aimed to determine the genetic diversity and phylogenetics of local duku and langsat (Lansium domesticum) based on matK gene DNA barcoding. DNA genome was extracted using Tiangen Plant Genomic DNA Kit DP305, then amplified using GoTaq Green Master Mix (Promega) and primers 3F_KIM-F and 1R_KIM-R. The product was sequenced by 1st Base Singapore and seven amplification results had a DNA size of 900 bp. The results showed that the matK gene is capable of determining Lansium domesticum interspecies. According to the DNA sequence phylogenetic analysis conducted using the maximum likelihood method, all the DNA samples were designated as Lansium domesticum Corr. with a fairly high genetic variation, indicated by DNA sequence differences caused by gene mutations. DNA Barcoding Menggunakan Gen Kloroplas MatK untuk Analisis Keragaman Genetik Tanaman Lansium domesticum Corr. yang Tumbuh di Sumatera Selatan, Indonesia ABSTRAK: Lansium domesticum Corr. yang dikenal dengan sebutan duku, langsat, dan kokosan merupakan buah yang populer di Indonesia, khususnya di Sumatera Selatan. Selain itu, buah ini digemari di daerah ini karena baunya yang harum, rasanya yang manis, dan bernilai ekonomis tinggi. Hingga saat ini, duku lokal Sumatera Selatan jarang diteliti, padahal informasi mengenai keragaman genetik plasma nutfahnya bermanfaat dalam mendukung program pemuliaan dan konservasi. Oleh karena itu, penelitian ini bertujuan untuk mengetahui keragaman genetic dan filogenetik duku dan langsat (Lansium domesticum) berdasarkan DNA barcoding gen matK. DNA genom diekstraksi menggunakan Tiangen Plant Genomic DNA Kit DP305, kemudian diamplifikasi menggunakan GoTaq Green Master Mix (Promega) dan primer 3F_KIM-F dan 1R_KIM-R. Produk tersebut disekuensing oleh 1st Base Singapore dan tujuh hasil amplifikasi memiliki ukuran DNA 900 bp. Hasil penelitian menunjukkan bahwa gen matK mampu mengidentifikasi Lansium domesticum antarspesies. Berdasarkan analisis filogenetik sekuens DNA yang dilakukan dengan metode kemungkinan maksimum, semua sampel DNA ditetapkan sebagai Lansium domesticum Corr. dengan variasi genetik yang cukup tinggi, ditunjukkan oleh perbedaan sekuens DNA yang disebabkan oleh mutasi gen.

Co-Authors Doni Setiawan Harmida Harmida Heri setiawan Ilmawati, Ika Juswardi Juswardi Juswardi, Juswardi Laila Hanum Mangandi, Wulan Fransisca Nina Tanzerina Nita Aminasih Nursela, Dewi Rina Sri Kasiamdari

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