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THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus Hamidah, -; Salni, -; Tanzerina, Nina
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.804 KB) | DOI: 10.24233/BIOV.3.1.2017.54

Abstract

Escherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 µg/mL for Escherichia coli and 500 µg/mL for  Staphylococcus aureus. The minimum  inhibitory concentration of the active  compound  to  Escherichia  coli  and  Staphylococcus  aureus  in  500  µg/mL.  The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin.
THE SECRETORY STRUCTURE OF ESSENTIAL OILS IN SOME SPECIES OF LAMIACEAE FOR TRADITIONAL COSMETICS OF BESEMAH TRIBES LAHAT’S DISTRICT Tanzerina, Nina; Aminasih, Nita; -, Erliah
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (906.323 KB) | DOI: 10.24233/BIOV.3.2.2017.82

Abstract

Besemah tribe uses plants in addition to the treatment is also used for beauty treatments traditionally. Based on Agustina's (2015) study, 109 species of plants were used for traditional medicine by the Tribe of Besemah. Some species of Lamiaceae for traditional medicine such as patchouli (Pogostemon cablin (Blanco) Benth.), wild mint (Mentha arvensis L.), hoary basil (Ocimum americanum L.), mexican mint (Plectranthus amboinicus (Lour.) Spreng.), and sweet basil (Ocimum basilicum L.) contains essential oils that can be used for traditional cosmetic ingredients. Essential oils are stored in the secretory structure of plants in leaf and stem organs. This study aims to determine the type and location of the secretory structure of essential oils in some species of lamiaceae used for traditional cosmetics of Besemah Tribes Lahat’s District. The research was conducted in November 2016 until February 2017. The results showed that patchouli plants was found in the presence of glandular trichomes, oil cells, and idioblast cells. The wild mint plants have glandular trichomes. The hoary basil plants have glandular trichomes and oil cells. The mexican mint plants have glandular trichomes and idioblast cells. The sweet basil plants have glandulartrichomes.Keywords: essential oils, lamiaceae, traditional cosmetics, Besemah tribes,  glandular trichomes, oil cells, idioblast cells
DIVERSITY OF EPIPHYTIC FERN ON THE OIL PALM PLANTS (Elaeis Guineensis Jacq.) IN CAMPUS OF SRIWIJAYA UNIVERSITY INDRALAYA Harmida, Harmida; Aminasih, Nita; Tanzerina, Nina
BIOVALENTIA: Biological Research Journal Vol. 4 No. 2 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.764 KB) | DOI: 10.24233/BIOV.4.2.2018.106

Abstract

Campus of  Sriwijaya University in Indralaya , has a high diversity of flora including fern, especially the epiphytic ferns, whose existence is so widely found in oil palm plants.  Survey about  diversity of this epiphytic has been carried out  from July to November 2017, with aim to identifying the diversity of species of epiphytic ferns in oil palm plants on the campus of UNSRI Indralaya. The research method used is exploration method with direct collection technique from the field and then made herbarium. A total of 27 species of pteridophytes were collected from the study area. Floristic analysis of collected specimens was carried out to find out the families, genus and species. In this survey was  collected 27 species belonged to13 genera, 7 families such  Aspleniaceae, Blechnaceae, Davalliaceae, Lygodiaceae, Nephrolepidaceae, Polypodiaceae, and Vittariaceae. The most common types are Polypodiaceae is Drynaria quercifolia, Drynaria sparsisora, Microsorum pustulatum, Microsorum punctatum, Phymatosorus scolopendria, Polypodium verrucosum, Polypodium polysthicum,  Pyrrosia piloselloides, and Goniophlebium verrucossum. The most widely distributed species in oil palm is Davallia denticulata , Goniophlebium verrucosum, and Nephrolepis biserrata.
MORPHOLOGICAL CHARACTERISTICS OF NEPENTHES IN PEAT SWAMP AREA OF TULUNG SELAPAN, SOUTH SUMATERA Wardana, Singgih Tri; Ilmawati, Ika; Tanzerina, Nina; Juswardi, Juswardi; Aminasih, Nita; Harmida, Harmida
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (640.485 KB) | DOI: 10.24233/BIOV.5.2.2019.152

Abstract

This study is aimed to recognise the morphological characteristics of Nepenthes mirabilis and Nepenthes gracilis in peat swamp area including stem, leaf, tendril, pitcher, flower, fruit, and seed.  This study has been conducted in January  until April 2018, located in Petaling Village Tulung Selapan Sub-District, Ogan Komering Ilir, South Sumatera. This study uses an exploratory method and the the observation sample using the method of random plot is chosen by purposive sampling. Parameters observed morphological characteristics of Nepenthes include stems, leaves, tendrils, pitcher, flower, adn fruit, as well as measurement of environmental factors. Based on the study, the researcher obtained two types of Nepenthes which are Nepenthes mirabilis and Nepenthes gracilis in  peat swamp area of Petaling Village Tulung Selapan Sub-District, Ogan Komering Ilir. Nepenthes mirabilis has a teres stem, leaves that in the form of oblong to lanceolate, has a petiolate, the edge of the leaves is flat covered by smoothy hair, thick peristome in size of 0,4-0,5 cm. Meanwhile stem Nepenthes gracilis has triangular stem, leaves in formed of lanceolate, sessile, flat edge of leaves, thin peristome in size of 0,1-0,2 cm. The pitcher shape of Nepenthes mirabilis and Nepenthes gracilis are cylindrical, infundibulate, and waist. The flower of Nepenthes mirabilis and Nepenthes gracilis are compound in type of  raceme inflorescences. The fruit is formed as capsula, has four lobed, and contains numerous seeds. The seed is spindle-like and white colored.
Peningkatan Kadar Protein Padi Melalui Mutagenesis Asam Amino Lisin Secara In Vitro Nina Tanzerina; Juswardi Juswardi
Jurnal Penelitian Sains No 13 (2003)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v0i13.296

Abstract

The research about metagenesis of lysine for increasing rice protein by in vitro had been conducted on July to November 2001, at Laboratory of Genetics and Biotechnology, Department of Biology, Faculty Mathematics and Natural Science, Sriwijaya University. The mutagenesis of lysine callus selected one step subculture with adding 2.0 mM of lysine on Murashige and Skoog medium. The total protein evaluated from the lysine mutant callus that growth and prolipheration on medium’s. The protein content measured with biuret reagent and bovine serum albumine as the standart protein. The result shown that no differences of colours, texture, and cell structure between lysine mutant and wild type (control) callus. The total protein increase to 71,53% on mutant lysine than wild type callus.
Akumulasi Prolin dan Indeks Toleransi Kalus Padi yang Diseleksi Polietilen Glikol (PEG) Secara In Vitro Sebagai Marka Toleransi Kekeringan Juswardi Juswardi; Nina Tanzerina
Jurnal Penelitian Sains No 14 (2003)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5566.434 KB) | DOI: 10.56064/jps.v0i14.294

Abstract

Proline accumulation and tolerance index in rice callus by in vitro selected on polyethylene glycol (PEG) as drought tolerance marker. The strait improvement of rice by in vitro has been done with selected callus rice on polyethylene glycol (PEG 6000). The research conducted to known proline accumulation and tollerance index in callus that selected by in vitro on PEG as tolerance index marker. Callus inducted from endosperm of cultivars Rojolele rice on MS medium with 10-5M 2,4-D. The selection of callus sub cultured step by step with add of PEG 250, 500 and 750 ppm on MS medium. Design of experiment used completely randomise design with two treatments that are selected callus and non selected calus and 10 replications. The observed on callus weight and proline content. Data analysed by t test for proline content and tolerance index for callus weight. The result shown that non significant differ on proline content if compare selected callus to non selected callus respectively 105,12% and 100%. Tolerance index of selected callus not differ from non-selected callus. Proline accumulation and tolerance index not yet guarantee as tolerance marker to  drought stress.
Konsentrasi Sludge Minyak Bumi dalam Proses Bioremediasi Memanfaatkan Bakteri Indigen dan Lamtoro Gung Arief Alghifari; Sri Pertiwi Estuningsih; Nina Tanzerina
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 4 No. 1 (2015): JLSO
Publisher : Research Center for Sub-optimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.27 KB) | DOI: 10.33230/JLSO.4.1.2015.142

Abstract

This research aimed to determine synergies between bacteria indigen with lamtoroin bioremediation processes at various concentrations of sludge through the measurement ofvariables, and the number of bacteria, impairment of Total Petroleum Hydrocarbons (TPH), andweight of lamtoro gung. Experimental design used was Complete Randomized Design (CRD) with5 treatments and 5 replications, increase the concentration sludge treatment is control (0%), 2.5%,5%, 7.5%, and 10% of sludge. The study found that given sludge concentration increases causingthe higher number of bacterial populations. Sludge concentration decreased significantly affectTPH values and highest TPH values decrease at 7,5% concentration of sludge. Sludgeconcentration significanly affect the wet weight and given treatment sludge, Lamtoro wet wieghtdecreased at the end ofthe study
Potency of endophytic fungi from Nauclea orientalis L. as antioxidant producer Elisa Nurnawati; Hary Widjajanti; Vivi Hendra Sutandar; Maulida Harwati; Eka Amelia; Siti Alharzsa; Nina Tanzerina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869//bphjbr.27.1.20216

Abstract

Nauclea orientalis L. is widely distributed in Indonesia. Secondary metabolites such as flavonoids, polyphenols, and saponins are abundant in the bark of N. orientalis L. These compounds have the potential to act as antioxidants. Endophytic fungi, through genetic transfer and coevolution, can produce the same metabolites as their host plant. As a result, understanding the potential of endophytic fungi from N. orientalis L. to produce antioxidant compounds that can be developed is critical. According to the findings of this study, twelve isolates have the potential to produce secondary metabolites with antioxidant properties. Three isolates had high antioxidant activity: DB2 was identified as Aspergillus minisclerotigenes with an IC50 of 21 g/mL containing tannins, terpenes, and flavonoids, AB3 as Colletotrichum perseae with an IC50 of 31 g/mL containing tannins and terpenoids, and AB1 as Diaporthe tulliensis with an IC50 of 48 g/mL containing tannins. The secondary metabolite group has the potential to be developed into an antioxidant agent
Potensi Neptunia oleracea Lour. pada fitoremediasi drainase asam tambang batubara Rika Nasution; Singgih Tri Wardana; Nina Tanzerina; Sri Pertiwi Estuningsih; Juswardi Juswardi
Sriwijaya Bioscientia Vol 1 No 2 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1124.275 KB) | DOI: 10.24233/sribios.1.2.2020.190

Abstract

Environmental problems in coal mining activities include acid mine drainage (AMD). Efforts to overcome the impact of AMD waste can be done by means of phytoremediation. Neptunia oleracea Lour. is one type of aquatic plant that has the potential to adapt and accumulate heavy metals. So it is necessary to do research with the aim of knowing the potential of N. oleracea in AMD phytoremediation. The phytoremediation potential of N. oleracea used a completely randomized design (CRD): without AMD as control (0%), with AMD concentrations of 25%, 50%, 75% and 100%. The results showed that N. oleracea has the potential to increase pH by 0.0322/day and reduce Fe content by 0.4760 mg/l/day, Mn 0.5776 mg/l/day, sulfate 0.4809 mg/l/day and reduce TSS by 0.0818 mg/l/day, at 100% AMD concentration. It can be concluded that N. oleracea has potential as an AMD phytoremediation agent to be developed in system constructed wetlands (CWs).
Studi etnobotani tumbuhan obat Suku Anak Dalam di Desa Sungai Jernih Kecamatan Rupit Kabupaten Musi Rawas Utara Sumatera Selatan Laila Sri Pujihastuti; Nina Tanzerina; Nita Aminasih
Sriwijaya Bioscientia Vol 1 No 2 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1398.255 KB) | DOI: 10.24233/sribios.1.2.2020.198

Abstract

The use of plants as traditional medicine has been widely known for a long time by the community, especially the Anak Dalam Tribe in Sungai Jernih Village, Rupit District Musi Rawas Utara Regency, South Sumatera. The purpose of this research is to inventoryof the types of medicinal plants, knowing family and habitus, types of diseases, plant organs used, processing methods and how to use plants used by the community as traditional medicine to treat various types of diseases. Method which conducted using a descriptive survey method by conducting interviews from three speakers who are traditional healers to help the process of collecting data in the field. The results showed that plants that were used as medicine by tribal children were found as many as 93 types of plants with 48 families and the largest families were Fabaceae and Piperaceae (6.45%) and were able to treat as many as 47 types of diseases with 116 medicinal ingredients. The largest plant habitats are shrubs, (32.25%) and as much as (60.21%) medicinal plants that’s grow wild both that grow wild in the forest and grow wild in the yard of the house. The most part of the plant organs used was the leaves (59.13%), the largest method of processing was boiling (67.74%) and the largest way of using it was drinking (61.29%%).