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All Journal Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi PREPOTIF : Jurnal Kesehatan Masyarakat Journal Microbiology Science Jurnal Pengabdian Farmasi dan Sains MPSJ
Siska Nuryanti, Siska
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Antibacterial Activity of Endophyte Fungi from Moringa Leaves (Moringa oleifera) in Gastrointestinal Infection Seminarwati, Sri; Nuryanti, Siska; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1026

Abstract

Moringa leaves (Moringa oleifera) have potential as an antibacterial because they contain tannins, steroids, triterpenoids, flavonoids, saponins, alkaloids, where these compounds can damage bacterial cell membranes. This study aims to determine the isolates of leaf endophytic fungi (Moringa oleifera) which have antibacterial activity against digestive tract infection bacteria using the agar diffusion method. The results of the isolation of the endophytic fungi of Moringa leaves (Moringa oleifera) obtained 6 isolates which were continued with purification and macroscopic examination. The pure isolates obtained were subjected to screening tests on the test bacteria. The isolate with the IFDK-05 code as the most active isolate, were then fermented. The fermented products were then filtered to separate the supernatant and mycelia. This supernatant was then tested for antibacterial activity using agar diffusion. The results of testing the antibacterial activity of moringa leaf endophytic fungi isolate using the diffusion method in order to obtain the diameter of the inhibition zone for Escherichia coli bacteria (9,01 mm), Salmonella thypi (10,70 mm), Shigella disentryae (9,13 mm), and Vibrio cholerae (-10,95mm). Based on the results of this study, isolates of endophytic fungi of Moringa leaves (Moringa oleifera) have antibacterial activity
Exploring The Antibacterial Potential of Aqueous and Ethanol Extracts from Bawang Dayak (Eleutherine palmifolia (L.) Merr) Against Skin Infectious Bacteria Through KLT-Bioautography Rusli, Rusli; Nuryanti, Siska; Putria, Yashifa Anandita
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1131

Abstract

Dayak onion plants (Eleutherine palmifolia (L.) Merr) have chemical compounds that have potential as antibacterials such as alkaloids, glycosides, flavonoids, phenolics, steroids and tannins. This study aims to determine the antibacterial activity of water extracts and ethanol extracts of dayak onion bulbs (Eleutherine palmifolia (L.) Merr). This research was conducted with the stages of sample extraction, screening of test bacteria, bioautogram profile testing and antibacterial activity by KLT-Bioautography method. Screening test results at a concentration of 1% against test bacteria Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis were able to inhibit the growth of test bacteria. Then continued with KLT-Bioautography testing using n-hexan: ethyl acetate (2: 1) eluent. The KLT-Bioautography test results showed that there was 1 active spot with an Rf value of 0.63 in the water extract and an Rf value of 0.60 in the ethanol extract against Pseudomonas aeruginosa, Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis bacteria. Based on the research, water extract and ethanol extract of dayak onion bulb (Eleutherine palmifolia (L.) Merr) have potential as antibacterial.Keywords: Antibacterial, KLT-Bioautography, Dayak Onion Bulbs
Synergistic Antibacterial Effects of Areca Nut Seed (Areca catechu L.) and Binahong leaf (Anredera cordifolia (Ten.) Steenis) Extracts Against Propionibacterium acnes Nuryanti, Siska; Nurung, Ayyub Harly; Saputra, Muh. Satrio Surya; Musfatir, Harvanita; Umaiyah, Ummi Putri
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1150

Abstract

Areca nut (Areca catechu L.) seeds has antibacterial properties due to their alkaloid, flavonoid, tannin, saponin, and polyphenol compounds. Binahong leaves (Anredera cordifolia (Ten.) Steenis) contain flavonoids, polyphenols, saponins, and alkaloids that also act as antibacterial agents. This study aimed to assess the antibacterial activity and synergistic effects of combining extracts from areca nut and binahong leaves against Propionibacterium acnes. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of P. acnes were evaluated for the ethanol extracts of areca nut and binahong leaves. The MIC and MBC values 400 ppm and 1.600 for extract ethanol single areca nut and 50.000 ppm and 100.000 ppm for binahong leaves. The MIC and MBC values for the combination 400/12.500 ppm and 1.600/781,25 ppm for areca nut and 50,000/800 ppm and 100,000/100 ppm for binahong leaves, respectively. The synergy between the two extracts was determined using the Fractional Inhibitory Concentration Index (FICI). The FICI value of 1. P. acnes indicates an indifferent effect (1 < FICI ≤ 4).
Aktivitas Antiinflamasi Ekstrak Etanol Dan Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) Dengan Menggunakan Metode Spektrofometri UV-Vis Suhaenah, Asriani; Nuryanti, Siska; Abidin, Zainal; Evatiara, Nur
Jurnal Fitofarmaka Indonesia Vol 11, No 2 (2024): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v11i2.1288

Abstract

Ficus is a species that is rich in polyphenolic compounds, such as flavonoids. These compounds are strong antioxidants that can aid in the prevention and treatment of various diseases caused by oxidative stress. They also protect cell structures, have anti-inflammatory properties, and can act as antibiotics. The aim of this study was to evaluate the anti-inflammatory properties of the ethanol extract and ethyl acetate fraction of rubber kebo (Ficus elastica) leaves using the Bovine Serum Albumin (BSA) protein denaturation method. The measurements were taken on a Uv-Vis spectrophotometer at a wavelength of 660 nm.  The study consisted of four experimental groups: a negative control, a positive control of diclofenac sodium, and test groups of ethanol extract and ethyl acetate fraction. The study revealed that the positive control (IC50) had an anti-inflammatory value of 9.2 μg/mL. Additionally, the ethanol extracts and ethyl acetate fractions of rubber kebo leaves (Ficus elastica) demonstrated anti-inflammatory activity with IC50 values of 31.24 μg/mL and 37.89 μg/mL, respectively. These findings suggest that the ethanol extracts and ethyl acetate fractions of rubber kebo leaves possess anti-inflammatory properties.
UJI AKTIVITAS EKSTRAK DAUN TIN (FICUS CARICA) SEBAGAI ANTIBAKTERI PADA INFEKSI MULUT Syamsu, Rachmat Faisal; Rachman, Moch. Erwin; Nuryanti, Siska; Akbar, Muhammad Alief; Ancha, Annisa
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 1 (2025): APRIL 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i1.40256

Abstract

Penyakit infeksi merupakan penyakit yang sering terjadi, baik yang menginfeksi anggota tubuh maupun pada rongga mulut. Hasil Riskesdas tahun 2018 bahwa proporsi masalah gigi dan mulut di Indonesia  sebanyak 57,6% tahun 2018 dengan proporsi terbesar adalah karies (45,3%). Infeksi rongga mulut di picu bakteri dominan Streptococci yakni spesies Streptococcus mutans. Di era sekarang perkembangan penelitian tentang kandungan dan manfaat pohon tin, baik daun, buah maupun akarnya sangat banyak. Ekstrak daun tin (Ficus carica .L) mengandung zat aktif seperti flavonoid, tanin, dan terpenoid telah dikenal memiliki potensi antibakteri. Berdasarkan hal tersebut peneliti ingin melakukan penelitian untuk melihat aktivitas ekstrak Daun Tin (Ficus Carica) sebagai antibakteri pada infeksi mulut yang belum pernah dilakukan sebelumnya dengan melibatkan mahasiswa. Jenis penelitian ini adalah penelitian desktiptif yang dilakukan dengan cara mengukur diameter zona hambat pada aktivitas antibakteri pada mulut seperti Streptococcus mutans. Pengujian aktivitas antibakteri dilakukan dengan metode difusi dan dilusi agar di laboratorium Mikrobiologi Fakultas Farmasi UMI. Hasil penelitian menemukan bahwa yakni nilai minimum inhibitor concentration (mic) yg dapat menghambat pertumbuhan bakteri Streptococcus mutans yaitu konsentrasi 6,4% dan Nilai minimum bactericidal concentration (mbc) yg dapat membunuh pertumbuhan bakteri Streptococcus mutans yaitu konsentrasi 12,8%. Sedangkan untuk konsentrasi terbaik dari ektrak etanol daun tin terhadap bakteri Streptococcus mutans ada pada konsentrasi 40% dengan diametar zona hambat 20,7 mm. Berdasarkan penelitian yang telah dilakukan dapat disimpulkan bahwa terdapat efek antibakteri dari ekstrak daun tin (Ficus carica) terhadap infeksi yang disebabkan bakteri di mulut yaitu Streptococcus Mutans (sm).
Antibacterial Activity of Etanol Extract of Pinang (Areca catechu L.) Clup against Streptococcus mutans and Porphyromonas gingivalis Nuryanti, Siska; Herwin, Herwin; Rahmayanti, Hani Rahmayanti
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1178

Abstract

Areca nut (Areca catechu L.) is a plant that has many uses and can be used for traditional medicine. Areca nut skin can be used to treat indigestion (dyspepsia), edema and beriberi due to low urine output. This study aims to determine the antibacterial activity of ethanol extract of areca nut peel against the test bacteria Streptococcus mutans and Porphyromonas gingivalis. In this research, the areca nut peel simplicia was extracted using the maceration method with 96% ethanol solvent, after which a liquid ethanol extract was obtained and then evaporated using a rotary vacuum evaporator to obtain a thick extract. After the extract is thick, a Minimum Inhibitor Concentration (MIC) test and a Minimum Bactericide Concentration (MBC) test are carried out. The results obtained in the Minimum Inhibitor Concentration (MIC) test and Minimum Bactericide Concentration (MBC) test on Streptococcus mutans and Porphyromonas gingivalis bacteria were 0.5%. Antibacterial activity test using the agar diffusion method was obtained, for Streptococcus mutans bacteria, the largest inhibitory zone diameter at a concentration of 16% was 12.58 mm and for Porphyromonas gingivalis bacteria at a concentration of 16%, namely 10.93 mm. Based on research, ethanol extract of areca nut peel has antibacterial potential.
Antimicrobial Activity of Ethanol Extract of Pinang Seed (Areca catechu L.) Against the Growth of Candida albicans and Malassezia furfur Fungi Using the Agar Diffusion Method Naid, Tadjuddin; Saputri, Febrianti Ayu; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1229

Abstract

Pinang seeds (Areca catechu L.) are known for their various benefits, including antimicrobial properties. This study aims to evaluate the antimicrobial activity of ethanol extract from pinang seed against the growth of Candida albicans and Malassezia furfur fungi using the agar diffusion method. The research involved extracting the pinang seed using maceration with 96% ethanol, followed by evaporation using a rotary vacuum evaporator to obtain a concentrated extract. Antimicrobial activity was tested across concentrations of 32%, 16%, 8%, 4%, 2%, 1%, 0.5%, and 0.1%. The results showed that at a concentration of 32%, the ethanol extract produced the largest inhibition zones: 27.78 mm against Candida albicans and 15.51 mm against Malassezia furfur, both categorized as very strong activity. These findings suggest that ethanol extract of pinang seed has significant potential as an antimicrobial agent, particularly against fungal pathogens.
Characterization of Cellulose-Producing Bacterial Isolates from the Areca Nut (Areca catechu L.) Nuryanti, Siska; Herwin, Herwin; hikma, Nurhikma
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1179

Abstract

Areca nut peel (Arecha catechu L.), a byproduct of areca nut processing, holds potential as a raw material for bioplastics through cellulose utilization. This study aimed to identify bacterial species in areca nut peel capable of producing cellulose. Employing bacterial isolation and identification methods, including morphological and biochemical tests, four bacterial isolates were obtained. Two isolates, identified as gram-negative, were classified under the genus Acetobacter, and two gram-positive isolates were identified as belonging to the genus Lactobacillus. These findings demonstrate the potential use of cellulose from areca nut peel in bioplastic production.
PELATIHAN PEMBUATAN INFUSED WATER DENGAN BAHAN DASAR REMPAH SEBAGAI PENINGKAT SISTEM IMUN Rusli; Nuryanti, Siska; Fitriana; Herwin; Nurung, Ayyub Harly
JURNAL PENGABDIAN FARMASI DAN SAINS Vol. 1 No. 2 (2023): April 2023
Publisher : Jurusan Farmasi FMIPA Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jpsf.2023.v1.i2.16243

Abstract

The corona virus pandemic (SARS-CoV-2) that causes COVID-19, which has entered Indonesia since early March, has not shown significant signs of ending. The Indonesian government continues to urge the public to always adopt a clean lifestyle, one of which is by consuming food or drinks that can boost the body's immune system. Infused water is mineral water that gets a mixture of various kinds of pieces of fresh fruit, vegetables, herbs or spices. This activity was carried out using the lecture method and direct discussions with the people of RT 07 RW 03, Sudiang Village, Makassar City, South Sulawesi. The target in this service is that participants can add knowledge about how to improve the immune system and the benefits of infused water as a way to boost the immune system and participants can find out about how to make infused water from spice-based ingredients.
EDUKASI TENTANG KOLESTEROL PADA IBU PKK DESA PADDINGING KECAMATAN SANROBONE KABUPATEN TAKALAR Amirah, Sitti; Kosman, Rachmat; Fitriana; Rahman, Safriana; Rusli; Putra, Bayu; Nuryanti, Siska; Harly Nurung, Ayyub
JURNAL PENGABDIAN FARMASI DAN SAINS Vol. 1 No. 2 (2023): April 2023
Publisher : Jurusan Farmasi FMIPA Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jpsf.2023.v1.i2.16306

Abstract

Cholesterol is a lipid compound needed by the body as an essential component of cell membranes, hormone synthesis, energy sources and other needs. The body needs lipids in moderate amounts, the average adult's cholesterol requirement is around 1100 mg of cholesterol/day. About 25-40% of cholesterol needs are obtained from food and the rest is obtained from synthesis by the body. High cholesterol levels can trigger atherosclerosis, hardening and narrowing of the arteries which can lead to various diseases such as ischemia, stroke, heart attack. By looking at the daily life of the people in Paddinding village, kec. Sanrobone, where the majority of farmers are farmers, allows the public to ignore information about health, so education is needed to improve public health status. This activity is carried out using lecture and discucussion methods that are focused on providing understanding to the public about cholesterol, what is the role of cholesterol in the body, sources of cholesterol, how to prevent and deal with cholesterol disorders. Community service activities carried out are one of the means to increase public knowledge about how to prevent and treat cholesterol disorders.
Co-Authors Akbar, Muhammad Alief Akihito mr, Andi Al Kahfi Alhijrah, Yayu Ancha, Annisa Anggraeni, Nur Afni Asriani Suhaenah Bayu Putra, Bayu Evatiara, Nur faridah, susanti Fitriana Fitriana Fitriana Hardiyanti, Vivi Herwin Herwin Herwin, Herwin hikma, Nurhikma Irfanitha, irfanitha Junardin, Nurul Fadhillah Kayla, Aulia Putri Mitra, Andi Musfatir, Harvanita Nurung, Ayyub Harly Ohorella, Fitriah Anggraini Priatna, Nabilah Hana Putra, Aryadi Surya Putria, Yashifa Anandita R. Rusli Rabbana, Rahmawati Rachman, Moch. Erwin Rachmat Faisal Syamsu, Rachmat Faisal Rachmat Kosman, Rachmat Rahman, Safriana Rahmayanti, Hani Rahmayanti Ramadhan, Wahyu Gilang Ramadhana, Evi Rahma Rosdah, Rosdah Rusli Saputra, Muh. Satrio Surya Saputri, Febrianti Ayu Selviati, Ratmi Seminarwati, Sri Sitti Amirah Soamole, Nurul Fatimah Tadjuddin Naid Umaiyah, Ummi Putri Zainal Abidin