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All Journal Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi PREPOTIF : Jurnal Kesehatan Masyarakat Journal Microbiology Science Jurnal Pengabdian Farmasi dan Sains MPSJ
Siska Nuryanti, Siska
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kayu Jawa (Lannea coromandelica (Houtt.) Merr.) Terhadap Bakteri Penyebab Infeksi Kulit Dengan Metode Difusi Agar Kayla, Aulia Putri; Fitriana, Fitriana; Nuryanti, Siska
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 3 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i3.40

Abstract

The purpose of this study was to determine the antibacterial activity of ethanol extract of Java wood leaves (Lannea coromandelica (Houtt.) Merr.) against bacteria that cause skin infections. The research method begins with an extraction process using the maceration method with 96% ethanol solvent and evaporated using a rotavapor. Java wood leaf ethanol extract was screened with a concentration of 0.1%; 0,5%; and 1% against bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes. MIC and MBC tests were carried out and antibacterial activity tests were continued using the agar diffusion method with a concentration variation of 0.8%; 1,6%; 3,2%; 6,4%; and 12.8%. Screening test results of Staphylococcus aureus bacteria at 0.5% and Propionibacterium acnes bacteria at 0.1%. MIC value against Staphylococcus aureus bacteria at 0.2% and Propionibacterium acnes bacteria at 0.05%. While the MBC value against Staphylococcus aureus bacteria at 0.8% and Propionibacterium acnes bacteria at 1.6%. The results of testing the antibacterial activity of ethanol extract of Java wood leaves against Staphylococcus aureus and Propionibacterium acnes bacteria had the largest inhibitory zone diameter at a concentration of 12.8% of 11.32 mm and 12.83 mm respectively. Based on the results of research, ethanol extract of Java wood leaves has potential as an antibacterial.
Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kayu Jawa (Lannea coromandelica (Houtt.) Merr.) Terhadap Bakteri Penyebab Infeksi Saluran Pencernaan Dengan Metode Difusi Agar Rabbana, Rahmawati; Kosman, Rachmat; Nuryanti, Siska
Makassar Pharmaceutical Science Journal (MPSJ) Vol 1 No 2 (2023)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v1i2.41

Abstract

The purpose of this research was to determine the antibacterial activity of the ethanol extract of Javanwood leaves Lannea coromandelica (Houtt.) Merr.) against bacteria that cause digestive tract infections. The research method begins with the extraction process using the maceration method with 96% ethanol solvent and evaporated using a rotary evaporator. Java wood leaf ethanol extract was subjected to screening tests with a concentration of 0.1%; 0.5%; and 1% against the test bacteria Eschericia coli, Salmonella typhi, Shigella dysenteriae and Vibrio cholerae. MIC and MBC tests were carried out and continued with antibacterial activity tests using the agar diffusion method with variations in concentrations of 0.5%; 1%; 2%; 4%; 8%; and 16%. The results of the screening test for Escherichia coli and Shigella dysenteriae bacteria at 0.5%. MIC values for Escherichia coli bacteria at 0.125% and Shigella dysenteriae bacteria at 2%. While the MBC value for Escherichia coli bacteria is at 0.5% and Shigella dysenteriae bacteria at 2%. The results of testing the antibacterial activity of the ethanol extract of Javanwood leaves against Escherichia coli and Shigella dysenteriae bacteria had the largest diameter of the inhibition zone at a concentration of 16%, respectively 17.10 mm; and 13.56 mm which is included in the criteria of having strong inhibition. Based on the results of the ethanol extract of Javanwood leaves, it has potential as an antibacterial.
Aktivitas Antibakteri Ekstrak Metanol Daun Bidara (Ziziphus mauritiana Lam) Terhadap Bakteri Uji Penyebab Luka Infeksi Dengan Metode Difusi Agar Irfanitha, irfanitha; Naid, Tadjuddin; Nuryanti, Siska
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i1.199

Abstract

Bidara leaves (Ziziphus mauritiana Lam) have the ability as an antibacterial, because they contain chemical compounds such as alkaloids, flavanoids, saponins, tannins and phenols. The content of phytochemical substances in bidara leaves accelerates the wound healing process on the outer skin. This study aims to determine the antibacterial activity of the methanol extract of bidara leaves (Ziziphus mauritiana Lam) against test bacteria that cause infectious wounds using the agar diffusion method. This test began with a screening test using 4 test bacteria and the results were inhibiting the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis and Propionibacterium acnes at concentrations of 0.1% and 0.5%. Then tested the antibacterial activity of the methanol extract of bidara leaves (Ziziphus mauritiana Lam) using the agar diffusion method and the result was that the ethanol extract of bidara leaves Ziziphus mauritiana Lam) has strong potential as an antibacterial against the test bacteria with the largest diameter of the inhibition zone at a concentration of 4% of 15 .00mm.
potensi ekstrak alga merah jenis Eucheuma cottoni sebagai antibakteri faridah, susanti; Nuryanti, Siska; Rusli, Rusli
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.232

Abstract

One type of algae cultivated in Indonesia is the red algae Eucheuma cottoni. Red algae are able to produce active metabolite ingredients to protect themselves from attacks by disease and predators. Initially, algae was only used for vegetables and it was not known what substances were contained in algae. As time goes by, knowledge about algae continues to grow. The red algae Eucheuma cottoni contains the chemical carrageenan, a polysaccharide compound produced from various types of red algae that has antimicrobial, anti-inflammatory, antipyretic, anticoagulant and other biological activities. This research aims to determine the potential of red algae extract as an antibacterial. This research uses a literature study approach where references are obtained through 4 data bases, namely Google Scholar, PubMed, Science Direct and Garuda, then based on the search, 8 journals are obtained that meet the inclusion and exclusion criteria. Based on the results of literature studies, it was found that red algae extract has antibacterial activity against the bacteria Salmonella thypi, Staphylococcus aureus, Staphylococcus epidermidis, Bacilus subtilis, and Escherichia coli. Key words: (Antibacterial, Eucheuma cottoni, Red Algae).
UJI SKRINING AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSAN DAUN SEREH (Cymbopogon citrantus) TERHADAP BAKTERI PENYEBAB INFEKSI KULIT Priatna, Nabilah Hana; Nuryanti, Siska; Rusli, Rusli
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.233

Abstract

Indonesia as a tropical country has a diversity of biological natural resources. This diversity is very beneficial, especially with the large number of plant species that can be used as medicine. One plant that is believed to be used as medicine is lemongrass which has lush and dense leaves. Lemongrass leaves (Cymbopogon citratus) is one of the medicinal plants and can be used as a kitchen spice. Lemongrass plants are very easy to find in Indonesia and contain alkaloids, saponins, tannins, polyphenols, flavonoids, and essential oils. Saponins and essential oils are the main group of chemicals that can exert activity against microbes. This study aims to conduct a screening test of antibacterial activity of the n-hexan fraction of lemongrass leaves (Cymbopogon citrantus) against bacteria that cause skin infections. This research method was conducted experimentally to test the antibacterial activity of lemongrass leaves by screening test. The results in this study, the n-hexan fraction of lemongrass leaves can inhibit the growth of test bacteria Staphylococcus aureus, Pseudomonas aureginosa, Staphylococcus epidermidis, and Propionibacterium acne. It can be concluded that lemongrass leaves (Cymbopogon citrantus) have antibacterial activity.
Detection of Salmonella Bacteria In Soybean Tempeh Produced In Palekko Village, Takalar Regency Anggraeni, Nur Afni; Naid, Tadjuddin; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1231

Abstract

Tempeh is a staple food in Indonesia, known for its nutritional benefits, including 25% protein, 5% fat, 4% carbohydrates, vitamin B12, and rich mineral content. Ensuring the safety and hygiene of tempeh is crucial, especially in preventing contamination by harmful bacteria like Salmonella, which poses serious health risks. This study aimed to detect the presence of Salmonella bacteria in tempeh produced in Palekko Village, Takalar Regency, using Salmonella Shigella Agar (SSA) medium. The method employed was experimental, involving the preparation and dilution of tempeh samples followed by incubation on SSA medium. The results is a no samples detected Salmonella met the Salmonella contamination limit requirements, which were negative/25g.
Antibacterial Activity of Ethanol Extract of Porang Tubers (Amorphophallus muelleri Blume) Against the Growth of Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313 and Vibrio Cholerae ATCC 14035 Bacteria Nuryanti, Siska; Nurung, Ayyub Harly; Putra, Aryadi Surya
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1358

Abstract

Porang tubers (Amorphophallus muelleri Blume) are known as local plants that contain active compounds such as glucomannan, saponins and flavonoids, which are thought to act as antibacterials. This study was conducted to determine the extent of the ability of ethanol extract of porang tubers to inhibit and kill Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313, and Vibrio cholerae ATCC 14035 bacteria, by looking at the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The extract was made using the maceration method using 96% ethanol, then tested for antibacterial properties using the liquid dilution method for MIC and the drop plate method for MBC. The results showed the MIC value for the three test bacteria at a concentration of 50,000 ppm. Meanwhile, the MBC value was not achieved even at the highest concentration, which was 100,000 ppm. Based on the results of this study, it can be concluded that the antibacterial effectiveness of ethanol extract of porang tubers is still relatively weak on Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313, and Vibrio cholerae ATCC 14035 bacteria.
Antifungal Activity of Cosmos caudatus Kunth Leaf Ethanol Extract Against Candida albicans Fitriana, Fitriana; Nuryanti, Siska; Ramadhan, Wahyu Gilang
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1366

Abstract

Ethanol extract of Kenikir leaves (Cosmos caudatus Kunth) is known to contain bioactive compounds such as flavonoids, tannins, saponins, and phenolics, which exhibit potential antibacterial and antifungal properties. This study aimed to evaluate ycandidiasis. Extraction was performed using maceration with 96% ethanol, yielding an extract with a 10.58% recovery rate. Preliminary screening at 0.1% concentration demonstrated fungal growth inhibition. Subsequent testing of Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) employed the solid dilution technique, revealing both MIC and MFC values at 0.1%. Antifungal activity was further assessed using the agar diffusion method at varying concentrations (2.5%, 5%, and 10%), with ketoconazole as a positive control. The largest inhibition zone (9.22 mm diameter) was observed at 10% concentration. Data were analyzed using one-way ANOVA followed by Tukey’s HSD test, revealing statistically significant differences between 2.5% and 10% concentrations, as well as between 5% and 10%. These findings demonstrate that C. caudatus ethanol extract possesses significant antifungal activity against C. albicans.
Potential of Antibacterial Producing Rhizosphere Bacteria from Porang Roots (Amorphophallus muelleri Blume) against Gastrointestinal Pathogens Nuryanti, Siska; Nurung, Ayyub Harly; Akihito mr, Andi Al Kahfi
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1342

Abstract

Increasing antibiotic resistance in gastrointestinal pathogens such as Escherichia coli, Shigella dysenteriae, and Vibrio cholerae encourages the search for new antimicrobial agents. This study aims to evaluate the antibacterial potential of rhizosphere bacteria from porang roots (Amorphophallus muelleri Blume) against the three pathogens through in vitro tests. Isolation was carried out from rhizosphere soil using a serial dilution technique. Eleven isolates were characterized morphologically and tested for antibacterial activity using the agar diffusion method. Two selected isolates (IBRAM-2 and IBRAM-11) were fermented and extracted with ethyl acetate. Antibacterial activity was evaluated using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests with the microdilution and drop plate methods, each with three replications and chloramphenicol as a positive control. The results showed an inhibition zone of 13.2–18.7 mm. The MIC value is 25,000 ppm, while the MBC value ranges from 25,000–50,000 ppm. Although it shows antibacterial activity, the high concentration required indicates weak antibacterial potential.
Co-Authors Akbar, Muhammad Alief Akihito mr, Andi Al Kahfi Alhijrah, Yayu Ancha, Annisa Anggraeni, Nur Afni Asriani Suhaenah Bayu Putra, Bayu Evatiara, Nur faridah, susanti Fitriana Fitriana Fitriana Hardiyanti, Vivi Herwin Herwin Herwin, Herwin hikma, Nurhikma Irfanitha, irfanitha Junardin, Nurul Fadhillah Kayla, Aulia Putri Mitra, Andi Musfatir, Harvanita Nurung, Ayyub Harly Ohorella, Fitriah Anggraini Priatna, Nabilah Hana Putra, Aryadi Surya Putria, Yashifa Anandita R. Rusli Rabbana, Rahmawati Rachman, Moch. Erwin Rachmat Faisal Syamsu, Rachmat Faisal Rachmat Kosman, Rachmat Rahman, Safriana Rahmayanti, Hani Rahmayanti Ramadhan, Wahyu Gilang Ramadhana, Evi Rahma Rosdah, Rosdah Rusli Saputra, Muh. Satrio Surya Saputri, Febrianti Ayu Selviati, Ratmi Seminarwati, Sri Sitti Amirah Soamole, Nurul Fatimah Tadjuddin Naid Umaiyah, Ummi Putri Zainal Abidin