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All Journal Jurnal Biologi dan Pembelajarannya (JBP) Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Anggun Wicaksono
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Agriculture, Biological Sciences & Forestry Education Health Professions Immunology & microbiology Mathematics

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Effect Of NAA And Kinetin Administration On Callus Induction In Vanilla Plant Stem Explants (Vanilla planifolia Andrews) Dhavin, Muhammad; Nurokhman, Amin; Ummi Hiras Habisukan; Yustina Hapida; Anggun Wicaksono; Weni Lestari; Arif Yachya
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.01.10574.%p

Abstract

Vanilla (Vanilla planifolia Andrews) is a plantation plant that is used for its fruit. Indonesia is one of the countries that exports vanilla and has climate factors that support increasing vanilla production to meet global demand, One of the efforts that can be made in vanilla propagation is through plant tissue culture. This research aims to induce calluses. This study used a Random Design using stem explants and several different hormone concentrations, namely: 0.0 ppm NAA and 0.0 ppm Kinetin, 1.0 ppm NAA and 0.5 ppm Kinetin, 1.0 ppm NAA and 1.0 ppm Kinetin, 2.0 ppm NAA and 0.5 ppm Kinetin, 2.0 ppm NAA and 1.0 ppm Kinetin, 2.0 ppm NAA and 2.0 ppm Kinetin. There was an effect on the administration of NAA and Kinetin on callus induction in the explants of the vanilla plant (Vanilla planifolia Andrews). It is based on the Anova one-way Hypothesis Test with a significance value of 0.000 (Asymp. Sig <0.05) then Ho was rejected and H1 was accepted. The optimal concentration to induce callus in stem explants is the administration of 1.0 ppm NAA + 0.5 ppm Kinetin with an average callus growth rate of 7 HST and a 100% callus growth percentage of explants.   Keywords: Vanilla, induction, callus, tissue culture, hormones
Preferensi Pakan Serangga Kumbang Daun (Epilachna varivestis) Dan Oteng-Oteng (Aulacophora similis) Dari Beberapa Jenis Tanaman Solanceae Faizah Qonita Zhafirah; Nadila Margaretha; Herlin Arlika; Okta Dea Riani; Anggun Wicaksono
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 11 No 1 (2024): APRIL 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v11i1.21741

Abstract

Insects are vertebrate animals classified in the phylum Arthropoda. Insects are one of the animals that are very numerous, one of which is in the insecta class, so the presence of insects really affects the ecosystem. This research aims to determine the food preferences of leaf beetles (Epilachna varivestis) and oteng- oteng (Aulacophora similis) from several types of Solanceae plants. The samples used in this research were the leaf beetle Epilachna varivestis and oteng-oteng (Aulacophora similis) and 5 types of Solanaceae plant leaves including cayenne pepper leaves, bird chili leaves, eggplant leaves, ciplukan leaves and takokak leaves. This observation was carried out to find out 1). How long does it take for the beetle to find its food? 2). Which type of leaf is eaten first and which type of leaf is eaten the most? 3). How long does it take for a beetle to eat this type of food? 4). Is there a switching of the beetle's eating pattern? Based on the results of observations made, the leaf beetle Epilachna sp apparently preferred cayenne pepper leaves (Capsicum frutescens) for 30 minutes slowly and stopped by the other four leaves but was not eaten by the leaf beetle, the same as the leaf beetle, Oteng-oteng (Aulacophora similis) only liked one leaf, namely preferring eggplant (Solanum melongena) leaves for 30 minutes slowly and stopped the other four leaves but the oteng-oteng didn't eat them.
Co-Authors Arif Yachya Dhavin, Muhammad Faizah Qonita Zhafirah Herlin Arlika Nadila Margaretha Nurokhman, Amin Okta Dea Riani Ummi Hiras Habisukan Weni Lestari Yustina Hapida