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All Journal Biogenesis: Jurnal Ilmiah Biologi Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Yustina Hapida
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Mathematics

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Neuronal cell death in the amygdala and cerebral cortex of mice (Mus musculus) induced by bee (Apis mellifera) venom Rian Oktiansyah; Ummi Hiras Habisukan; Anggun Wicaksono; Yustina Hapida; Fahmy Armanda
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.36165

Abstract

Brain is an organ to control our activities, such as human consciousness, emotional, and movement. It was controlled by amygdala and cerebral cortex as the parts of the brain. Many things that can bring over it. Bee venom (BV) is known as traditional medicine and probably can sway the brain. The objective of this study was to determine the dose of BV that causes excessive neuronal cells death, especially in the amygdala and cerebral cortex. About 15 white male mice Deutsch Denken Yoken (DDY) strain were divided into control group and the treatment group. BV was administrated intraperitoneally for two weeks with multilevel doses, that was 1.88 mg/kg, 3.76 mg/kg, 5.6 mg/kg, and 7.48 mg/kg. Brain tissue isolation was performed three days of the last administration by using perfusion method. Morphological sectioned of brain tissue (amygdala and brain cortex) was stained by hematoxylin-eosin (HE). The results indicated that the BV inclined to affect neuronal cells death in the amygdala and cerebral cortex. Based on the study, the highest doses (7.48 mg/kg) of BV caused the highest neuronal cell death.
Effect Of NAA And Kinetin Administration On Callus Induction In Vanilla Plant Stem Explants (Vanilla planifolia Andrews) Dhavin, Muhammad; Nurokhman, Amin; Ummi Hiras Habisukan; Yustina Hapida; Anggun Wicaksono; Weni Lestari; Arif Yachya
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.01.10574.%p

Abstract

Vanilla (Vanilla planifolia Andrews) is a plantation plant that is used for its fruit. Indonesia is one of the countries that exports vanilla and has climate factors that support increasing vanilla production to meet global demand, One of the efforts that can be made in vanilla propagation is through plant tissue culture. This research aims to induce calluses. This study used a Random Design using stem explants and several different hormone concentrations, namely: 0.0 ppm NAA and 0.0 ppm Kinetin, 1.0 ppm NAA and 0.5 ppm Kinetin, 1.0 ppm NAA and 1.0 ppm Kinetin, 2.0 ppm NAA and 0.5 ppm Kinetin, 2.0 ppm NAA and 1.0 ppm Kinetin, 2.0 ppm NAA and 2.0 ppm Kinetin. There was an effect on the administration of NAA and Kinetin on callus induction in the explants of the vanilla plant (Vanilla planifolia Andrews). It is based on the Anova one-way Hypothesis Test with a significance value of 0.000 (Asymp. Sig <0.05) then Ho was rejected and H1 was accepted. The optimal concentration to induce callus in stem explants is the administration of 1.0 ppm NAA + 0.5 ppm Kinetin with an average callus growth rate of 7 HST and a 100% callus growth percentage of explants.   Keywords: Vanilla, induction, callus, tissue culture, hormones
Co-Authors Anggun Wicaksono Anggun Wicaksono Arif Yachya Dhavin, Muhammad Fahmy Armanda Nurokhman, Amin Rian Oktiansyah Ummi Hiras Habisukan Weni Lestari