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Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements Fahira, Nurul Rifqah; Latunra, Andi Ilham; Johannes, Eva
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 39 No. 3 (2023)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v39i3.565

Abstract

Robusta coffee (Coffea canephora) is Indonesia's essential leading trade commodity. Robusta coffee is preferred for its bitter taste. However, robusta coffee productivity decreased due to bad agriculture practice. Tissue culture is an alternative to cultivation with various advantages. The plant growth regulators (PGRs) influence tissue culture’s success. Due to its stability, 2,4 dichlorophenoxyacetic acid has become a common synthetic PGRs. PGRs can be obtained from natural ingredients, such as tomatoes. This study aimed to analyze the effect of 2,4-dichlorophenoxyacetic acid and tomato extract addition and its appropriate concentration for callus induction of robusta coffee in vitro. This research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This study used a Complete Randomized Design with two factors. The first factor was the concentration of tomato extract (0%; 7.5%; 10%; and 12.5%). Meanwhile, the second factor was the concentration of 2,4 dichlorophenoxyacetic acid (0 ppm; 1 ppm; 2 ppm; and 3 ppm). Observation parameters include the percentage of callus formation, callus growing time, callus fresh weight, callus color, and callus texture. The quantitative data were analyzed by the Kruskal-Wallis test followed by the Mann-Whitney Test to compare the effect of each treatment. The results showed that among other treatment combinations, treatment with the addition of 2 ppm 2,4-D and 10% tomato extract (T2D2) and treatment with the addition of 3 ppm 2,4-D and 10% tomato extract (T2D3) had the best effect in inducing the callus of robusta coffee.
Optimization of Quillbot Utilization in The Preparation of Scientific Papers by Teachers at SMA Negeri 2 Parepare, South Sulawesi Rahmi, Nur; Ekasasmita, Wahyuni; Miftahulkhairah, Miftahulkhairah; Fajri S, Ahmad; Lawi, Armin; Sir, Muhammad Mochsen; Latunra, Andi Ilham; Anggitamaya, Anggitamaya; Amal, Muhammad Ikhlasul
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 9 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i9.7487

Abstract

Writing scientific work is an important indicator of the development of knowledge and critical thinking. But still, many teachers have difficulty writing scientific papers, including avoiding plagiarism. The dedication to the society conducted by the Bacharuddin Institute of Technology team Jusuf Habibie aims to improve the skills of teachers at 2 Parepare State High School in reducing the risk of plagiarism in the writing of scientific works through optimization of the use of QuillBot and implementation of paraphrase techniques in writing. The method used is ABCD (asset-based community development). The activities include identification of needs, planning based on problem analysis, preparation of training material books, training on paraphrase techniques and QuillBot, and evaluation of writing quality as well as the effectiveness of training by participants. The training was held on June 6, 2024, followed by 26 participants. The average pretest for the Similarity Index (SI) writing percentage of participants was 54.62%, while the average posttest value for the participant's SI writing percent was 37.85%, with an average SI decrease of 16.77%, showing this training has proven to be effective in reducing the rate of plagiarism among participants. A total of 21 of the 26 participants, or 80.8% of the participants, succeeded in reducing the level of plagiarism in their writings. Thus, this dedication program succeeds in helping teachers improve their skills in writing scientific papers to avoid plagiarism.
The potential of coffee husk biochar for chromium removal from acid mine drainage using constructed wetlands Titania, Opi Mida; Fahruddin, Fahruddin; Latunra, Andi Ilham
Journal of Degraded and Mining Lands Management Vol. 13 No. 1 (2026)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2026.131.9587

Abstract

This study aimed to characterize physicochemical properties of biochar generated from coffee husks and assess its efficacy in the removal of chromium (Cr) from acid mine drainage (AMD) using a constructed wetland system. The biochar was made through pyrolysis and then chemically activated with H?PO? and KOH to enhance its adsorption. Characterization results showed increased surface area and porosity, and that functional groups such –OH, –COOH, and C=O were present, which are good for metal binding. In the experiment, 5 g of biochar (T2) reduced the amount of Cr from 59.53 ppm to 4.47 ppm in 12 days, which was better than other amounts of biochar applied. The study observed significant reductions in sulfate content and improvements in pH. The sulfate levels in T2 went down from 176.78 mg/L to 81.60 mg/L, while the pH increased from below 3 to 6.85. This shows that the acid was neutralized well. These improvements show that biochar not only absorbs contaminants but also lowers the acidity of AMD. The addition of mangrove sediment also enhanced the remediation process by using ion exchange, complexation, and microbial activity, especially from sulfate-reducing bacteria (SRB). The results show that coffee husk biochar, especially when combined with mangrove sediment, is a sustainable and eco-friendly material for treating heavy metal-contaminated acidic wastewater.