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ISOLASI, SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN FUNGI ENDOFIT TANGKAI DAUN MURBEI (Morus alba L.) Irwandi Irwandi; Ratih Arum Astuti
JURNAL ETNOFARMASI Vol. 1 No. 02 (2022): JURNAL ETNOFARMASI
Publisher : Universitas Pendidikan Muhammadiyah Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36232/jurnalfarmasiunimuda.v1i02.1731

Abstract

Fungi endofit merupakan fungi yang hidup di bagian dalam tanaman yang berpotensi penghasil metabolit sekunder yang mirip atau sama dengan tanaman inangnya. Penelitian ini bertujuan untuk mengidentifikasi isolat fungi dari tangkai daun murbei (Morus alba L) memiliki aktivitas antioksidan dan menentukan golongan senyawa metabolit sekunder dari tangkai daun murbei (Morus alba L) yang aktif sebagai antioksidan. Isolasi fungi endofit dilakukan dengan teknik isolasi langsung pada medium PDAC (Potato Dekstrosa Agar) ditambahkan kloramfenikol 0,2 g/L, selanjutnya dilakukan pemurnian isolat dan didapatkan 2 isolat yaitu isolat MAIRP dan MAIRH. Kemudian dilakukan fermentasi menggunakan medium PDY, setelah itu diekstraksi menggunakan etil asetat. Ekstrak etil asetat selanjutnya dilakukan uji kualitatif menggunakan lempeng KLT setelah itu disemprotkan DPPH 0,04 mM. Hasil yang didapatkan yang mempunyai aktivitas Antioksidan yaitu isolat MAIRP. Hasil uji kuantitatif pengujian aktivitas Antioksidan IC50 298,044 µg/mL yang termasuk kategori sedang. Hasil dari skrining fitokimia ekstrak etil asetat hasil fermentasi fungi yang diisolasi dari tangkai daun murbei (Morus alba L.)Diduga kuat mengandung golongan senyawa alkaloid, flavonoid, dan polifenol.
FORMULASI DAN EFEKTIVITAS SEDIAAN GEL PEWARNA RAMBUT ALAMI EKSTRAK BUAH MERAH (Pandanus conoideus L.) Muhammad Andy Irfan; Irwandi Irwandi; A.M. Muslihin
JURNAL ETNOFARMASI Vol. 3 No. 01 (2025): JURNAL ETNOFARMASI
Publisher : Universitas Pendidikan Muhammadiyah Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36232/jurnalfarmasiunimuda.v3i01.1960

Abstract

Synthetic dyes are a type of dye that contains chemical substances as their main component, and to date, they remain a primary choice in hair coloring processes due to their excellent color results and long-lasting durability. However, the discovery of several cases of contact dermatitis resulting from the use of synthetic hair dyes has highlighted the potential side effects of these dyes. One common effect is irritation to the scalp and hair. Red fruit (Pandanus conoideus L.) is a Papua-specific plant rich in carotenoids. To facilitate its use, red fruit can be formulated into a hair dye gel preparation. Gel is chosen for its practicality, ease of absorption, formation of a film layer that is easy to clean, and provision of a cooling effect on the skin. The stages of this research include sample preparation, formulation of hair dye gel at concentrations of 6%, 8%, and 10%, physical characteristic testing, and effectiveness testing of the red fruit ethanol extract hair dye gel preparation. The results showed that 141.9 grams of thick extract were obtained from 750 grams of dry simplicia, resulting in a yield of 70.45%. The physical characteristic and effectiveness testing of the hair dye gel preparation at concentrations of 6%, 8%, and 10% were satisfactory. The ethanol extract of red fruit can be formulated into a hair dye gel preparation at concentrations of 6%, 8%, and 10%, with comparable coloring results.