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Immunological parameters of dental alloy corrosion; A study of gingival inflammation after placement of stainless steel crown Indriyanti, Ratna
Padjadjaran Journal of Dentistry Vol 20, No 3 (2008): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

The dental alloy is widely used in many fields of dentistry as a restoration material, orthodontic, prosthodontic, oral surgery and endodontic treatments. Naturally, most of the metallic materials without exception to stainless steel alloy will experience a process of corrosion in a form of electrochemical reaction to achieve thermodynamic equilibrium. The corrosion process in the oral cavity is due to the reaction of metal with saliva as an oral cavity electrolyte fluid. SSC has preformed restoration material conform with dental anatomy, manufactured from stainless steel alloy which is formable and adaptable to the teeth. Stainless Steel Crown generally made of austenitic stainless steel 18/8 of AISI 304 group contain chrome 18% and Nickel 8%, can be used as a restoration for teeth with excessive caries, crown fracture, email hypoplasia, or restoration after endodontic treatment. The toxic effect of Ni+2 released due to corrosion process may cause an inflammation of the gingiva and periodontal tissue. Laboratorically this condition indicated by the expression of pro-inflammation cytokines as immunological parameters such as IL-6, IL-8, TNF and IL-1Î² whose main role is to initiate and enhance any inflammation responses. The presence of pro-inflammation cytokines can be detected as soon as 1 hour after placement of SSC by examination of gingival crevicular fluid (GCF) by ELISA technique. The magnitude of the toxic effect depends on corrosion rate and ions release which is influenced by metal chemical composition, environment temperature and pH, metal wear due to abrasion and friction, soldering if any, and elongation of the metal. Conclusion: The release of Ni+2 during corrosion process after placement of SSC cause gingival inflammation which is indicated by the change of the immunological parameters.

An illustration of speech articulation impairment in children with cerebral palsy tested by the Goldman-Fristoe method Rusmarini, Ade Pungky; Haroen, Edeh Rolleta; Indriyanti, Ratna
Padjadjaran Journal of Dentistry Vol 21, No 1 (2009): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Seventy percent of children with cerebral palsy were found to suffer from speech articulation impairment. The purpose of this research was to obtain an illustration of speech articulation impairÂment in children with cerebral palsy tested by the Goldman-Fristoe method at the SLB-D School for DisÂabled Children Bandung in 2007. This was a descriptive research. Sampling was carried out by purposive sampling. The speech articulation impairment test was carried out on the basis of the Goldman-Fristoe method, that is, an articulation test which places the consonant at the beginning, middle, and at the end of a word, to test speech articulation impairment in children with cerebral palsy. Research results indicated that speech articulation impairment in the bilabial consonants /p/,/b/, and /m/ is the average 85.51%. Speech articulation impairment of the labiodental consonants /f/ and /v/ is an average 89.13%. Speech articulation impairment of the alveolar or dental consonants /t/ and /d/ is an average of 80.43%. Speech articulation impairment in the palatal consonants /c/ is an average of 82.60%. Speech articulation impairment in velar consonants /k/ and glottal consonants /h/ is an average of 86.96%. ReÂsearch results indicated that more than three-fourths of children with cerebral palsy at the SLB-D School for Disabled Children Bandung in 2007 suffered from speech articulation impairment.

Daya antibakteri asam palmitat bawang putih (Allium sativum) terhadap Streptococcus mutans ATCC 25175Antibacterial activity of garlic (Allium sativum) palmitic acid towards Streptococcus mutans ATCC 25175 Nadya Oktarina Hendy; Ratna Indriyanti; Meirina Gartika
Padjadjaran Journal of Dental Researchers and Students Vol 4, No 2 (2020): Oktober 2020
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v4i2.27595

Pendahuluan: Bawang putih sering digunakan masyarakat untuk mengobati berbagai penyakit termasuk yang disebabkan oleh bakteri. Bawang putih (Allium sativum) mengandung asam palmitat yang diduga memiliki aktivitas antibakteri terhadap Streptococcus mutans melalui lisis membran sitoplasma bakteri dan dapat menghambat aktivitas kerja enzim bakteri. Tujuan penelitian ini untuk mengetahui daya antibakteri asam palmitat bawang putih (Allium sativum) terhadap pertumbuhan bakteri Streptococcus mutans ATCC 25175. Metode: Penelitian bersifat deskriptif. Analisis kemurnian isolat menggunakan kromatografi lapis tipis (KLT), kemudian dilakukan uji zona hambat terhadap Streptococcus mutans menggunakan metode difusi agar Kirby-Bauer pada medium agar Mueller Hinton. Kontrol penelitian adalah klorheksidin. Zona hambat yang terbentuk di sekitar paper disk diukur menggunakan jangka sorong. Hasil: Hasil KLT menunjukkan adanya senyawa asam palmitat sebagai senyawa aktif. Asam palmitat bawang putih pada konsentrasi 0,5%, 2%, 4%, 8%, dan 12% tidak memiliki zona hambat terhadap Streptococcus mutans dan memperlihatkan gumpalan putih berupa asam lemak di sekeliling paper disk. Zona hambat klorheksidin menunjukkan rata-rata 18 mm. Simpulan: Asam palmitat bawang putih (Allium sativum) tidak memiliki daya antibakteri pada konsentrasi yang 0,5%, 2%, 4%, 8%, dan 12% terhadap Streptococcus mutans ATCC 25175. Kata kunci: Zona hambat asam palmitat bawang putih, Streptococcus mutans ATCC 25175, disc diffusion. ABSTRACTIntroduction: Garlic is often used by people to treat various diseases, especially bacterial infection disease. Garlic (Allium sativum) contains palmitic acid, which is assumed to have the antibacterial activity against Streptococcus mutans through bacterial cytoplasmic membrane lysis and inhibits the activity of bacterial enzymes. The purpose of this study was to determine the antibacterial activity of garlic (Allium sativum) palmitic acid on the growth of Streptococcus mutans ATCC 25175. Methods: This research was descriptive. Purity analysis of the isolated compound of palmitic acid was performed using a thin layer chromatography (TLC). The inhibition zone test of Streptococcus mutans was carried out afterwards, using the Kirby-Bauer diffusion method on the Mueller Hinton agar medium. The research control was chlorhexidine. A calliper measured the inhibition zone formed around the paper disk. Results: The TLC results showed that the isolated active compound was palmitic acid. Garlic palmitic acid in the concentrations of 0.5%, 2%, 4%, 8%, and 12% did not have inhibitory zone towards Streptococcus mutans and showed white lumps of fatty acids around the paper disk. Chlorhexidine showed an inhibition zone with an average of 18 mm. Conclusion: Garlic (Allium sativum) palmitic acid has no antibacterial activity towards Streptococcus mutans ATCC 25175.Keywords: Inhibitory zone of garlic palmitic acid, Streptococcus mutans ATCC 25175, disc diffusion.

PERBEDAAAN PENURUNAN MASA BIOFILM Streptococcus mutans ANTARA PEMBERIAN FRAKSI N-HEKSANA DAN ETIL ASETAT EKSTRAK ETANOL BAWANG PUTIH SIUNG TUNGGGAL (Allium sativum L.) Ratna Indriyanti; Faizal Hasan; Meirina Gartika
ODONTO : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.6.0.21-28

Background: Garlic (Allium sativum L) is one of the most important Allium species consumed worldwide and has been used for decades as a cure for various diseases. The aim of this research was to compare the efficacy of single-clove garlic’s n-hexane and ethyl acetate fractions in inhibiting the growth of Streptococcus mutans biofilmMethod: This was a true experimental research. The biofilm mass was determined by absorbance value at 590 nm wavelength with ELISA reader in a microplate using safranin. Data was analyzed using ANOVA followed by post hoc analysis with ρ value <0.05.Result: The result showed that average decrease of biofilm mass after 1-30 minutes of ethyl acetate fraction administration was 33.4 ± 8.03 mg/mL, n-hexane 23.6 ± 0.97 mg/mL and chlorhexidine 35.5 ± 0.98 mg/mL. The average decrease of biofilm mass after 30-60 minutes of ethyl acetate fraction administration was 11.94 ± 7.44 mg/mL, n-hexane 43.87 ± 41.6 mg/mL and chlorhexidine 16.35 ± 4.6 mg/ mL. Statistic analysis showed that there was a notable difference in the decrease of Streptococcus mutans biofilm mass (ρ value= 0.00). Conclusion: The conclusion of this research was that administrating either n-hexane or ethyl acetate fractions of a single-clove garlic has the ability to decreaseS. mutans biofilm mass.

Effectiveness of Applied Behavior Analysis (ABA) with regard to tooth brushing in autistic children Felicia Melati; Ratna Indriyanti; Arlette Suzy Setiawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 3 (2019): September 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p117-121

Background: Children demonstrating autistic spectrum disorders tend to be uncooperative when receiving dental treatment. Actions as simple as brushing the teeth with a prophylactic brush can constitute complex processes for children with such conditions. Applied behavior analysis (ABA) can train children in new positive behavior and it is, therefore, anticipated that an ABA-based approach iscapable of influencing the behavior of individuals with autistic spectrum disorder. Purpose: This study aimed to assess the effectiveness of the ABA-based approach for autistic children during dental brushing procedures. Methods: The research constituted a quasiexperimental single subject investigation of children presenting autistic spectrum disorders who attended the Lembaga Pendidikan Autisma Prananda, Bandung. Potential changes in the behavior of subjects were monitored four times during treatment with a one-week interval between consultations. Those subjects satisfying the inclusion criteria consisted of 11 boys and 4 girls. The data analysis used in this study consisted of an ANOVA test and a non-parametric Kruskal-Wallis test with a p–value < 0.005. Results: Changes in scores between the initial and final consultations were statistically significant with a p-value (0.269) <0.05. Statistically significant differences existed between changes in the behavior of level 1 and level 2 autistic subjects. Conclusion: An ABA-based approach effectively changes the behavior of autistic children with regard to prophylactic brushing. Children with level 1 autistic spectrum disorder demonstrate greater capacity to follow instructions and consistently implement a prophylactic brushing technique.

An illustration of speech articulation impairment in children with cerebral palsy tested by the Goldman-Fristoe method Ade Pungky Rusmarini; Edeh Rolleta Haroen; Ratna Indriyanti
Padjadjaran Journal of Dentistry Vol 21, No 1 (2009): March 2009
Publisher : Faculty of Dentistry Universitas Padjadjaran

Seventy percent of children with cerebral palsy were found to suffer from speech articulation impairment. The purpose of this research was to obtain an illustration of speech articulation impairment in children with cerebral palsy tested by the Goldman-Fristoe method at the SLB-D School for Disabled Children Bandung in 2007. This was a descriptive research. Sampling was carried out by purposive sampling. The speech articulation impairment test was carried out on the basis of the Goldman-Fristoe method, that is, an articulation test which places the consonant at the beginning, middle, and at the end of a word, to test speech articulation impairment in children with cerebral palsy. Research results indicated that speech articulation impairment in the bilabial consonants /p/,/b/, and /m/ is the average 85.51%. Speech articulation impairment of the labiodental consonants /f/ and /v/ is an average 89.13%. Speech articulation impairment of the alveolar or dental consonants /t/ and /d/ is an average of 80.43%. Speech articulation impairment in the palatal consonants /c/ is an average of 82.60%. Speech articulation impairment in velar consonants /k/ and glottal consonants /h/ is an average of 86.96%. Research results indicated that more than three-fourths of children with cerebral palsy at the SLB-D School for Disabled Children Bandung in 2007 suffered from speech articulation impairment.

Role of P27kip1 Protein, P45skp2 Coactivator, and P38jab1 Coactivator in Preventing Rhabdomyosarcoma (RMS) in Oral Cavity of Children Inne Suherna Sasmita; Ratna Indriyanti; Willyanti Soewondo
Majalah Kedokteran Bandung Vol 54, No 2 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n2.259

Rhabdomyosarcoma is a rare malignant tumor that attacks the differentiation of skeletal muscle and usually affects children, contributing to about 60% of all soft tissue sarcomas. The purpose of this study was to examine the relationship between p27Kip1 immunoexpression and p45Skp2 and p38Jab1 coactivators, as well as the relationship between p27Kip1 immunoexpression and p45Skp2 and p38Jab1 coactivators on stages and prognosis of oral RMS in children. This was a restrospective study on the immunoexpression of p27Kip1 and p45Skp2 and p38Jab1 coactivators on RMS cells. The RMS stage was determined according to the American Joint Committee of Cancer/AJCC of stages 1–4, and were divided into group I (stages 1 and 2) and group II (3 and 4). Samples were retrieved fromt he paraffin blocks of patients with embryonal RMS. Each paraffin block was cut, and 6 samples with 5 µm thickness from each block were examined using p27Kip1, p45Skp2, and p38Jab1 proteins. The analysis was performed using a linear regression test on the relationship between p27Kip1 and p45Skp2 and p38Jab1, resulting in a p-value of 0.000 (<0.05) and a coefficient value of b -1.36. Meanwhile, the stage was analyzed using the Wald test of 8.0688, resulting in a p-value of 0.0045 with a significant negative correlation. Analysis on the relationship between p45Skp2 and p38Jab1 and the RMS stage was performed using the Gamma test, resulting in a significant positive correlation (p<0.05).

LEBAR SALURAN NAFAS FARING DAN POSISI TULANG HYOID PASIEN MALOKLUSI SKELETAL KELAS I DAN II PADA RADIOGRAF SEFALOMETRI Nova Rosdiana; Suhardjo Sitam; Farina Pramanik; Ratna Indriyanti
Cakradonya Dental Journal Vol 13, No 2 (2021): Agustus 2021
Publisher : FKG Unsyiah

Lebar saluran nafas faring dapat dipengaruhi pola skeletal wajah dan posisi tulang hyoid. Tujuan penelitian ini adalah untuk menganalisis perbedaan lebar saluran nafas faring atas, bawah, dan posisi tulang hyoid antara pasien maloklusi skeletal kelas I dan kelas II ditinjau menggunakan radiograf sefalometri. Penelitian ini berupa deskriptif analitik yang menggunakan arsip data sekunder radiograf sefalometri pasien di Instalasi Radiologi Kedokteran Gigi RSGM UNPAD. Sampel penelitian ditentukan dengan metode purposive sampling dengan jumlah 44 arsip radiograf sefalometri. Rata- rata lebar saluran nafas faring atas pasien maloklusi skeletal kelas I 14,81±4,08 mm dan kelas II 12,27±3,16 mm dengan nilai P 0,0026 (P 0,05). Rata-rata lebar saluran nafas faring bawah pasien maloklusi skeletal kelas I 11,66±2,57 mm dan kelas II 10,62±1,77 mm dengan nilai P 0,202 (P0,05). Posisi tulang hyoid kelas I dan kelas II menunjukkan hasil yang sama, 7 posisi segitiga positif dan 15 segitiga negatif dengan nilai P 1,000 (P0,05). Terdapat perbedaan signifikan lebar saluran nafas faring atas antara pasien maloklusi skeletal kelas I dan kelas II ditinjau menggunakan radiograf sefalometri. Tidak ditemukan perbedaan signifikan lebar saluran nafas faring bawah dan posisi tulang hyoid.

PERBEDAAAN PENURUNAN MASA BIOFILM Streptococcus mutans ANTARA PEMBERIAN FRAKSI N-HEKSANA DAN ETIL ASETAT EKSTRAK ETANOL BAWANG PUTIH SIUNG TUNGGGAL (Allium sativum L.) Ratna Indriyanti; Faizal Hasan; Meirina Gartika
Odonto : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Garlic (Allium sativum L) is one of the most important Allium species consumed worldwide and has been used for decades as a cure for various diseases. The aim of this research was to compare the efficacy of single-clove garlic’s n-hexane and ethyl acetate fractions in inhibiting the growth of Streptococcus mutans biofilmMethod: This was a true experimental research. The biofilm mass was determined by absorbance value at 590 nm wavelength with ELISA reader in a microplate using safranin. Data was analyzed using ANOVA followed by post hoc analysis with ρ value <0.05.Result: The result showed that average decrease of biofilm mass after 1-30 minutes of ethyl acetate fraction administration was 33.4 ± 8.03 mg/mL, n-hexane 23.6 ± 0.97 mg/mL and chlorhexidine 35.5 ± 0.98 mg/mL. The average decrease of biofilm mass after 30-60 minutes of ethyl acetate fraction administration was 11.94 ± 7.44 mg/mL, n-hexane 43.87 ± 41.6 mg/mL and chlorhexidine 16.35 ± 4.6 mg/ mL. Statistic analysis showed that there was a notable difference in the decrease of Streptococcus mutans biofilm mass (ρ value= 0.00). Conclusion: The conclusion of this research was that administrating either n-hexane or ethyl acetate fractions of a single-clove garlic has the ability to decreaseS. mutans biofilm mass.

LEBAR SALURAN NAFAS FARING DAN POSISI TULANG HYOID PASIEN MALOKLUSI SKELETAL KELAS I DAN II PADA RADIOGRAF SEFALOMETRI Nova Rosdiana; Suhardjo Sitam; Farina Pramanik; Ratna Indriyanti
Cakradonya Dental Journal Vol 13, No 2 (2021): Agustus 2021
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v13i2.23529

Lebar saluran nafas faring dapat dipengaruhi pola skeletal wajah dan posisi tulang hyoid. Tujuan penelitian ini adalah untuk menganalisis perbedaan lebar saluran nafas faring atas, bawah, dan posisi tulang hyoid antara pasien maloklusi skeletal kelas I dan kelas II ditinjau menggunakan radiograf sefalometri. Penelitian ini berupa deskriptif analitik yang menggunakan arsip data sekunder radiograf sefalometri pasien di Instalasi Radiologi Kedokteran Gigi RSGM UNPAD. Sampel penelitian ditentukan dengan metode purposive sampling dengan jumlah 44 arsip radiograf sefalometri. Rata- rata lebar saluran nafas faring atas pasien maloklusi skeletal kelas I 14,814,08 mm dan kelas II 12,273,16 mm dengan nilai P 0,0026 (P 0,05). Rata-rata lebar saluran nafas faring bawah pasien maloklusi skeletal kelas I 11,662,57 mm dan kelas II 10,621,77 mm dengan nilai P 0,202 (P0,05). Posisi tulang hyoid kelas I dan kelas II menunjukkan hasil yang sama, 7 posisi segitiga positif dan 15 segitiga negatif dengan nilai P 1,000 (P0,05). Terdapat perbedaan signifikan lebar saluran nafas faring atas antara pasien maloklusi skeletal kelas I dan kelas II ditinjau menggunakan radiograf sefalometri. Tidak ditemukan perbedaan signifikan lebar saluran nafas faring bawah dan posisi tulang hyoid.

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