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All Journal At-Taqaddum THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Biologi Tropis Conference on Innovation and Application of Science and Technology (CIASTECH) Klinikal Sains (Jurnal Analis Kesehatan) JURNAL KESEHATAN PERINTIS Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Jurnal Peduli Masyarakat Indonesian Journal of Global Health research International Journal of Health Science and Technology
Muhammad Taufiq Qurrohman, Muhammad Taufiq
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : International Journal of Health Science and Technology

 1 
Comparison of DNA isolation of Candida albicans with filter-based kit and cooling methods Muhammad Thoriq Aulia Khamim; Qurrohman, Muhammad Taufiq
International Journal of Health Science and Technology Vol. 5 No. 2 (2023): November
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/ijhst.v5i2.3273

Abstract

Candidiasis is a disease caused by the fungus Candida sp.,  which can infect the skin, hair, mucous membranes, and internal organs. Laboratory diagnosis  of Candida albicans can be done molecularly with the aim of identifying fungal DNA through DNA isolation as an initial stage. This study used an experimental research design used to compare two DNA isolation methods, namely filter-based kits and cooling. DNA purity is assessed by measuring absorbance at 260 nm and 280 nm. Qualitative evaluation is carried out through visualization of DNA bands in electrophoresis. After conducting five DNA re-isolation experiments for both filter-based kit and cooling methods, the highest DNA concentrations were observed in samples isolated using filter-based kits, with a result of 6.465 μg/μL. In addition, an adequate level of purity is achieved with this method. In addition, thicker and lighter bands of DNA are observed, which can be attributed to the use of the enzyme Lyticase and other chemicals. The variability of isolation results can be affected by several factors, including extraction speed, sample type, extraction method, and environmental conditions. Technical errors during measurements can also affect the amount of DNA. In comparison, DNA isolation using filter-based kits for Candida albicans resulted in superior quantity and quality of DNA compared to cooling methods.  
Quality and quantity of Aspergillus niger DNA isolation using filter-based kit and cooling method Renatasya Silviana Herningtyas; Qurrohman, Muhammad Taufiq
International Journal of Health Science and Technology Vol. 6 No. 1 (2024): July
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/ijhst.v6i1.3274

Abstract

Polymerase Chain Reaction (PCR) is an important technique to improve sensitivity in the detection of fungal infections, such as those caused by Aspergillus niger. The availability of pure DNA and DNA isolation techniques are important factors in implementing PCR. This study aims to compare the quality and quantity of DNA isolation of Aspergillus niger using the Filter Based Kit method and cooling. The experimental research design was used with a qualitative test of DNA isolate using Agarose Gel Electrophoresis (1.5%) and a quantitative DNA test using a UV-Vis Spectrophotometer (wavelengths of 260 nm and 280 nm). Data analysis compared the qualitative and quantitative results of DNA isolates from both methods. The results showed the presence of DNA bands in both isolation methods, with thicker bands in the Filter Based Kit method. The average concentration of DNA after isolation using the Filter Based Kit (6,478 ng/μl) was higher than that of the cooling method (5,994 ng/μl). The purity of DNA was also higher in the filter-based kit (1.7) than in the cooling method (1.1). The Filter Based Kit method contains chemical components that support the successful isolation of DNA. It can be concluded that the filter-based kit method produces Aspergillus niger DNA isolate with better quality and quantity than the cooling method. The implication of these findings is that the Filter Based Kit could be a better option for the isolation of Aspergillus niger DNA in laboratory applications.
Co-Authors Annisa, Mariana Suryanti Nur Ariyanti Ariyanti Cahyanti, Eva Dewangga, Vector Stephen Dewangga, Vector Stephen Dhiya, Salsabila Shafa Exzora, Helmalia Bilqis Fitri, Syifa Aurelya Hastuti, Novita Dwi Khasanah, Defi Karmila Uswatun Maharani, Raras Muhammad Thoriq Aulia Khamim Murwani, Antik Ningrum, Anik Puspita Noviana Dewi Priyandari, Damar Ajeng Pujianti, Yunita Putri Putra, Muhammad Andriyansyah Sanky Putri, Adila Purnama Putri, Alya Ernita Rahmawati, Desinta Ramadhan, Intan Nur Rasid, Bryan Aditya Nur Renatasya Silviana Herningtyas Rizki, Andita Ainal Sanita, Hanifah Velda Sari, Monica Ayu Retno Sawitri, Debora Sayekti, Fitria Diniah Janah Shaleha, Annisa Azizah Sitohang, Dahlan Srikandini, Chris Tambunan, Yohana Nathalia Wijayanto, Mustofa Tri Yasinta, Tia Yoga Pratama Putra