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Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java Rokhmani, Rokhmani; Setyawati, Endang Ariyani; Wahyono, Daniel Joko
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.11720

Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its bodyâs morphological variations, not yet molecular. A research has been conducted to identify molekuler of the Trichodina spp. with the infecting 18S rRNA gene marking on the gourami larvae in Exs. Residence of Banyumas, Central Java. The research used a survey method with the samples of gourami. Amplification of 18S rRNA gene from Trichodina heterodentata was Performed using PCR technique. Primer used is Forward primer (5 â-AAC CTG GTT GAT CCT GCC ATG-3â) and Reverse primer (5 â-TGA TCC TTC TGC AGG TTC ACC TAC-3â) which produces a 600 pb amplicon of DNA. Molecular research can be a complementary identification of organisms morphologically. Amplification of the partial 18S rRNA gene may be used to identify Trichodina specifically. Gourami larvae taken from the hatchery centers in Banyumas, Purbalingga, and Banjarnegara. The results show that the detected percentage of Trichodina heterodentata genes with the infecting 18S rRNA gene marking on the gourami larvae in Central Java taken from the hatchery centers in Banyumas, Purbalingga and Banjarnegara are respectively 10%, 10%, and 45%. This research provides a benefit in mapping the presence of protozoa pathogen of Trichodina spp. in gourami hatcheries in the Former Exs. Residence of Banyumas, Central Java

SPESIFISITAS PARASIT PROTOZOA Trichodina sp. PADA GURAME, TAWES, NILEM DAN NILA YANG DIBUDIDAYAKAN POLIKULTUR Utami, Prasetyarti; Rokhmani, Rokhmani
Jurnal Harpodon Borneo Vol 9, No 2 (2016): Volume 9 No 2 Oktober 2016
Publisher : Jurnal Harpodon Borneo

Parasites Protozoa Trichodina sp. have the ability to divide the rapid reproduction, spread wide, and can infect more than one species of fish. Fish farmers in Banyumas is still prevalent cultivate carp polyculture together with Tawes, nilem and tilapia. Research was conducted to determine the specificity of protozoan parasites Trichodina sp. certain that infects each cultivated fish in polyculture. The research method is a survey. Sampling sites in the village, Banyumas Regency Baturaden Kutasari and implementation of isolation and identification of parasites conducted at the Laboratory of Parasitology- Entomology Biology Faculty Unsoed Purwokerto. The results based on measurements of the variation morfomertrik for determination of species Trichodina sp. found on each fish is no difference or no spesifisitas. Species Trichodina sp. found in carp, Tawes, nilem and farmed tilapia polyculture is the same, Trichodina nigra, Trichodina acuta, Trichodina Trichodina nobilis and heterodentata. Keywords : Trichodina sp., Specificity, Aquaculture, polyculture

STUDI KASUS ANISAKIASIS PADA BEBERAPA IKAN LAUT DI TEMPAT PELELANGAN IKAN CILACAP SEBAGAI SALAH SATU RUJUKAN KUALITAS DAGING IKAN LAUT HASIL TANGKAPAN Rokhmani, Rokhmani
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 2 No 2 (2009): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6004.381 KB)

Analisakiasis were one of parasite diseases at sea fishs which caused of larva stadium III infection anisakid, and infected to human being (Zoonosis). Infection at human being happened if human being eat less raw or matured sea fish concidering stadium larva of III (L3) Aniasakid in its, and will result eosinofilik granuloma the hardness at human being intestines. The research about anasakid larva at some sea fishes were conducted at Cilacap. The Method of these research was survey with purposive random sampling randome. Location sample intake was conducted at Auction Fish ; PPSC, Teluk Penyu and Lengkong Cilacap. Amount of fishs sampel taking was 5% from day haul. Fish type of sampel which in taking was belanak, flatulent, and bean. Inspection of fish conducted in Entomology ? Parasitology Laboratoty of Biology Faculty of Jenderal Soedirman University Purwokerto, The examination was conducted to checking organ in, body cavity and flesh concerning to make ensure that was no anisakis Sp. in it. The result showed high percentage of anisakis sp were found at fish of belanak, flatulent, and bean were equal to 83,3%, 91,7% and 100%. Body cavity at most found anisakis sp. most found at body cavity in state of ganging or capsulated.  

Prevalensi dan Variasi Morfometrik Trichodina sp. pada Benih Ikan Gurami (Osphronemus gouramy Lac.) di Desa Rajapolah Tasikmalaya Mukholladun, Wildan; Rokhmani, Rokhmani; Riwidiharso, Edy
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3220

Gurami (Osphronemusgouramy Lac.) Is a type of freshwater fish that is widely cultivated by the people of Indonesia. The high price of gouramy is a measure of consumption and the meat is tender and tasty. In Tasikmalaya, carp farmers in the cultivation of gouramy seeds are still experiencing problems. Gouramy seeds raised to adult size experience high mortality due to ectoparasites. One of the ectoparasites was Trichodina sp. Research has been conducted aimed at 1. Knowing the prevalence of gouramy seeds that were attacked by Trichodina sp. obtained from fish farmers in the village of Rajapolah, Tasikmalaya Regency and 2. Knowing the morphometric variations of Trichodina sp. found in gouramy seeds obtained from fish farmers in Rajapolah Village, Tasikmalaya Regency. The method used in this study is a survey method. Seed gourami aged 2 weeks is use as a sample. Isolation and identification were carried out by making a range preparation and painting using a 2% AgNO3 solution. The results found that the prevalence of 88% or high, while the results of the examination of morphometric variations found 4 species of trichodina sp. namely T. Pediculus, T. Nigra, T.heterodentata, and T. Acuta.Keywords:Trichodina sp., Gourami, morphometric variations, abundance, Rajapolah

Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java Rokhmani, Rokhmani; Wahyono, Daniel Joko; Mulyani, Lilis
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3924

Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.

Prevalensi Infestasi Cacing Gastrointestinal Pada Kambing (Capra sp.) Yang Dipelihara Secara Intensif Dan Semi Intensif Khirqah, Aulia; Rokhmani, Rokhmani; Riwidiharso, Edy
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4224

Bahaya akibat infestasi cacing gastrointestinal sangat merugikan pertumbuhan dan perkembangbiakan budidaya kambing. Penelitian ini bertujuan untuk mengetahui jenis cacing, prevalensi dan intensitas telur cacing gastrointestinal pada kambing yang dipelihara secara intensif dan semi intensif, serta mengetahui perbandingan prevalensi dan intensitas cacing gastrointestinal berdasarkan jenis kelamin dan usia kambing. Penelitian ini dilakukan pada peternakan kambing intensif dan semi intensif di Desa Klareyan, kecamatan Petarukan, Pemalang dengan metode survey purposive random sampling pada bulan November 2020. Jenis cacing gastrointestinal yang ditemukan pada kambing yang dipelihara secara intensif yaitu Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., dan Ascaris sp., sedangkan jenis cacing yang ditemukan pada kambing yang dipelihara secara semi intensif yaitu Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., Ascaris sp., Moniezia expansa, Moniezia benedeni, Fasciola sp., dan Schistosoma sp. Intensitas telur cacing gastrointestinal pada kambing yang dipelihara secara intensif yaitu 1184,44±1743,283 lebih tinggi dibandingkan dengan intensitas telur cacing pada kambing yang dipelihara secara semi intensif yaitu 1109,50±1573,737 (P>0,05). Prevalensi infestasi cacing gastrointestinal pada kambing yang dipelihara intensif yaitu 90%, lebih rendah dibandingkan dengan prevalesi telur cacing pada kambing yang dipelihara secara semi intensif dengan prevalensi 100% (Sign. 0,147). Prevalensi dan intensitas cacing gastrointestinal pada kambing jantan lebih kecil dibandingkan pada kambing betina (Sign. 0,189; P>0,05). Selain itu prevalensi dan intensitas telur cacing gastrointestinal pada kambing anakan lebih besar dibandingkan pada kambing medium dan dewasa (Sign. 0,241; F<0,05). Kata kunci: Helminthiasis, Intensif, Kambing, Semi intensif.

Prevalensi dan Kelimpahan Ektoparasit pada Ikan Belanak (Mugil Cephalus) Hasil Tangkapan di Muara Sungai Serayu Adipala Cilacap Pajrulloh, Ade Rizki; Rokhmani, Rokhmani; Riwidiharso, Edy
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4733

Belanak (Mugil cephalus) is a fish that can adapt to a wide salinity 17-30% and its distribution in tropical to subtropical areas, so that mullet can be found in rivers to river mouths, one of which is found in the Serayu River Estuary, Adipala Cilacap. Fish diseases can be caused by parasites, one of which is ectoparasites. Ectoparasites are organisms that benefit from infesting the body surface of fish. This study aims to determine the prevalence of mullet infested with ectoparasites and to determine the abundance of ectoparasites in mullet (Mugil cephalus) in the Serayu Adipala River, Cilacap. The research method used is survey method with purposive random sampling technique. The variables observed were the prevalence and abundance of ectoparasites. The parameters observed were the type and number of ectoparasites found in mullet. The results showed that the prevalence of ectoparasites in mullet, which was 31±13.216%, was included in the general infestation category. The types of ectoparasites found were Dactylogyrus sp. and Apiosoma sp. The abundance of ectoparasites found in mullet is Dactylogyrus sp. of 0.25±0.14 ind/head and Apiosoma sp. of 0.36± 0.14 ind/head. Both results included in the category of very rare infestations and the highest ectoparasites found in the organs of the mullet fish were found in the gills, namely Dactylogyrus sp. as many as 25 individuals.

Jenis dan Kelimpahan Ektoparasit pada Ikan Budidaya di Balai Benih Ikan Sidabowa Banyumas Jawa Tengah Rokhmani, Rokhmani; Prihastina, Marlina Yoga; Setyowati, Endang Ariyani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 2 (2024): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.2.11613

Balai Benih Ikan (BBI) Sidabowa merupakan salah satu Balai Benih Ikan di Banyumas yang terletak di Kecamatan Patikraja Kabupaten Banyumas. Komoditas ikan yang dibudidayakan di BBI Sidabowa adalah ikan tawes (Barbonymus ganionotus) dan ikan nila (Oreochromis niloticus). Pada pembudidayaannya ada satu tantangan yaitu serangan penyakit. Salah satu penyakit yang menyerang ikan adalah ektoparasit. Penelitian ini bertujuan untuk mengetahui jenis ektoparasit apa saja yang menyerang ikan tawes dan ikan nila serta mengetahui kelimpahan ektoparasit yang menyerang ikan tawes dan ikan nila di BBI Sidabowa, Banyumas. Metode penelitian yang digunakan yaitu metode survei dengan teknik pengambilan sampel random sampling. Parameter yang diamati adalah jenis dan jumlah ektoparasit. Kelimpahan ektoparasit ikan tawes dan ikan nila dengan menghitung jumlah individu ektoparasit yang ditemukan dibagi jumlah ikan sampel yang diamati. Hasil penelitian menunjukkan bahwa ektoparasit yang ditemukan pada ikan tawes dan ikan nila terdapat empat jenis ektoparasit yaitu Ichthyophthirius multifilis, Trichodina sp, Dactylogyrus sp dan Gyrodactylus sp. Kelimpahan ikan tawes pada Ichthyophthirius multifilis (17,66), Dactylogyrus sp (0,28), dan Gyrodactylus sp (0,07). Kelimpahan ikan nila pada Trichodina sp (2,44), Dactylogyrus sp (0,15), dan Gyrodactylus sp (0,08).

Integrasi Sustainable Development Goals (SDGD) dalam Problem Based Learning: Sebuah Pendekatan Untuk Penguatan Keterampilan Berpikir Kritis Baadilla, Irwan; Hidayatullah, Ahmad; Rokhmani, Rokhmani; Zamzam, Rohimi
Ranah: Jurnal Kajian Bahasa Vol 14, No 2 (2025): Ranah: jurnal Kajian Bahasa
Publisher : Badan Pengembangan dan Pembinaan Bahasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26499/rnh.v14i2.8411

This study aims to analyze the effect of implementing Problem-Based Learning (PBL) integrated with the Sustainable Development Goals (SDGs) on improving students’ critical thinking skills in the Indonesian language course. The research employed a quasi-experimental method using a Nonequivalent Control-Group Design involving two classes: an experimental class applying PBL-SDGs and a control class using the lecture method. Data were collected through tests to measure students’ initial (pretest) and final (posttest) critical thinking abilities. Data analysis techniques included instrument validity and reliability tests, descriptive analysis, assumption tests (normality and homogeneity tests), paired sample t-test, and independent sample t-test. The results showed that the average critical thinking score of students in the experimental class increased more significantly than that of the control class (86.04 vs. 77.36) with a significance value of 0.000 < 0.05. The findings confirm that the integration of PBL-SDGs has a significant impact on critical thinking skills. This result encourages students to be more active in identifying problems, analyzing information, constructing arguments, and formulating contextual solutions. The study concludes that the implementation of SDG-based PBL is more effective than the lecture method in enhancing critical thinking while also fostering students’ critical awareness of global sustainability issues. Abstrak Penelitian ini bertujuan untuk menganalisis pengaruh penerapan Problem-Based Learning (PBL) yang diintegrasikan dengan Sustainable Development Goals (SDGs) terhadap peningkatan keterampilan berpikir kritis mahasiswa pada mata kuliah Bahasa Indonesia. Penelitian menggunakan metode quasi eksperimen dengan desain Nonequivalent Control-Group Design pada dua kelas, yaitu kelas eksperimen dengan penerapan PBL-SDGs dan kelas kontrol dengan metode ceramah. Teknik pengumpulan data dilakukan dengan tes untuk mengukur kemampuan awal (pretes) dan kemampuan akhir (postes) berpikir kritis mahasiswa. Teknik analisis data dalam penelitian ini meliputi uji validitas dan reliabilitas instrumen, analisis deskriptif, uji prasyarat (uji normalitas dan homogenitas), paired sample t-test, dan independent sample t-test. Hasil penelitian menunjukkan bahwa skor rata-rata kemampuan berpikir kritis mahasiswa kelas eksperimen meningkat lebih signifikan dibanding kelas kontrol (86,04 vs 77,36) dengan nilai signifikansi 0,000 < 0,05. Temuan ini menegaskan bahwa integrasi PBL-SDGs memiliki pengaruh terhadap keterampilan berpikir. Hasil ini mendorong mahasiswa lebih aktif mengidentifikasi masalah, menganalisis informasi, menyusun argumen, serta merumuskan solusi kontekstual. Penelitian ini menyimpulkan bahwa penerapan PBL berbasis SDGs lebih efektif dibanding metode ceramah dalam meningkatkan berpikir kritis sekaligus menumbuhkan kesadaran kritis mahasiswa terhadap isu global keberlanjutan.

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