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All Journal Journal of Innovation and Applied Technology Jurnal Sumberdaya Akuatik Indopasifik Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Akuakultura Universitas Teuku Umar Sewagati Biodiversitas Journal of Biological Diversity Ganec Swara
I Made Dedi Mahariawan
Department Of Aquaculture, Faculty Of Fisheries And Marine Science, University Of Brawijaya, Malang, Jalan Veteran, Ketawanggede, Lowokwaru, Malang, Jawa Timur, 65145, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Physics Public Health Social Sciences Other

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Performance of Amino Acid Supplementation in Feed on the Production of Cantang Grouper (Epinephelus lanceolatus x Epinephelus fuscoguttatus) in the Nursery Phase Rahardjo, Seto Sugianto Prabowo; Kurniawan, Hendra Agung; Oktaviani, Elsa Dian; Wibawa, Gigih Setia; Widyawati, Yuni; Mahariawan, I Made Dedi
JURNAL AKUAKULTURA Vol 8, No 2 (2024): Jurnal Akuakultura Universitas Teuku Umar
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/ja.v8i2.10200

Abstract

The growth of Cantang grouper is influenced not only by genetic factors but also by the quality of feed, particularly the protein content, which is closely related to amino acid levels. This study aims to investigate the effect of adding commercial amino acids to feed on the growth of Cantang grouper. The research was conducted using an experimental method with a Randomized Block Design (RBD), comprising four treatments and three replicates of amino acid doses added to commercial feed: P0 (0%), P1 (1%), P2 (2%), and P3 (3%). The parameters observed included survival rate, weight growth rate, length growth rate, specific growth rate, and feed conversion ratio. The results showed that adding amino acids to commercial feed over a 21-day rearing period significantly affected all parameters (p<0.05). The highest survival rate, weight growth, length increase, and specific growth rate were found in the P2 treatment with the values of 95.00 ± 5.00%, 7.40 ± 0.24 g, 3.69 ± 0.75 cm, 9.83 ± 0.15%/day, respectively. In addition, the lowest feed conversion ratio was obtained in the P2 treatment (1.22 ± 0.03).
Implementation of Laserpuncture, Microdiet, and Synbiotic in Fish Farming Community in Boyolali Regency Hariati, Anik Martinah; Mahariawan, I Made Dedi; Rahardjo, Seto Sugianto Prabowo; Hariyadi, Hariyadi; Suciati, Lusia Dyah; Nugroho, Nurul; Putri, Ajeng Ayu Restuaning; Permatasari, Felyta Dian; Salsabila, Sivana Yesica Ayu; Satria, Ibnu Darma; Denanta, I Made Kresna; Dailami, Muhammad
Journal of Innovation and Applied Technology Vol 11, No 1 (2025)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2024.010.002.13

Abstract

The community service program in Boyolali Regency aims to increase the productivity of the fisheries sector through assistance with laserpuncture technology, the application of microdiet and natural feed, and the production of synbiotics. The methods used include counseling and direct practice involving more than 40 local fish farmers. The results of the program show that the application of laserpuncture and nutritional engineering through microdiet and synbiotics tecknology are increase fish growth. Participation and positive responses from the community show high enthusiasm for new technology which has a direct impact on productivity and the local economy. It is hoped that this program can be implemented independently in sustainable fisheries businesses.
Dinamika Populasi Bakteri Pada Budidaya Ikan Koi (Cyprinus rubrofuscus) Sistem Resirkulasi Mahariawan, I Made Dedi; Andayani, Sri; Suprastyani, Heny; Sa’adati, Feni Tri
Jurnal Sumberdaya Akuatik Indopasifik Vol 8 No 1 (2024): Februari
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2024.Vol.8.No.1.406

Abstract

Koi fish (Cyprinus rubrofuscus) is one of the aquaculture commodities that is highly marketable because of its unique color pattern and body shape. The prevalence of parasites and pathogenic agents in Koi fish among farmers poses challenges and leads to the occurrence of numerous illnesses. Water quality management is implemented in an effort to prevent several infectious microorganisms. The application of a recirculation system is used to enhance water quality and bolster the disease resistance of Koi fish. The objective of this study was to evaluate the application of RAS technology in the koi fish culture under different filter media on bacterial density. This study used four treatments, namely treatment A (RAS with a dacron filter and pumice), B (RAS with a dacron filter and sand), C (RAS with a sand and pumice filter), and K (control without a recirculation system). The parameter observed in this study was the density of bacteria in the koi fish rearing-media. These findings demonstrated that the utilization of various filters had an impact on bacterial density. Treatment C (RAS with sand and pumice filter) exhibited the greatest bacterial density with an average value exceeding 3.4 log CFU. mL-1 from the first week to the third week. The density of nitrifying bacteria in the koi fish production system is positively influenced by the high-quality maintenance practices that are maintained under optimal conditions.
Optimization of Temperature and Drying Time of Encapsulated Synbiotic Powder on the Characteristics and Viability of Microcapsules Lintang Dion Pangestu; I Made Dedi Mahariawan; Ating Yuniarti; Anik Martinah Hariati
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9979

Abstract

The drying process is often a problem in maintaining the stability of probiotics and the quality of microcapsules in the processing of synbiotic microencapsulation. This study aims to optimize the temperature and drying time to maintain the core material's stability and the microcapsules' quality. The research design in this study was a completely randomized design with two factorials, namely drying temperature (30°C, 35°C, and 40°C) and drying time (2, 2.5, and 3 hours). The results showed that the highest viability and efficiency values ​​were obtained in the drying process at 40°C for 2 hours, which were 9.29 log CFU/mL and 98.10%. In addition, the water content and gel strength of the microcapsules obtained also showed optimal conditions. Based on the results obtained, it can be concluded that the drying process at 40°C for 2 hours is the best treatment, with the highest viability and EE values ​​and low water content values.
The Effect of Different Guanidino Acetic Acid (GAA) Levels and Protein Source on Blood Profile of Tilapia Fish (Oreochromis niloticus) Fakhirah Oktafiani Afifah; I Made Dedi Mahariawan; Wahyu Endra Kusuma; Bagus R. Hidayat; Ating Yuniarti2; Anik Martinah Hariati
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.7608

Abstract

Tilapia (Oreochromis niloticus) is a freshwater fish that has high economic value and potential to be cultivated. The problem that often arises in tilapia cultivation is the lack of optimal use of the feed given. The purpose of this research was to find out blood profile of tilapia during culture period. The method used was a completely randomized design (CRD) with five treatments and every treatment with four replications. This study used 240 Tilapia with average weight of 15 g. The stocking density of the aquaria is 15 fish. Test feed in this study is the form of dry pellets consisting combination of GAA levels, protein content (25% dry matter bases), and source of protein (fish meal and non-fishmeal). During the cultured period of 70 days, fish fed by formula diet of 3% biomass per day. Sampling for blood profile tested of erythrocytes, leukocytes, and hematocrit is carried out after the research. The water quality of temperature, pH, and DO were daily monitoring. The combination treatment of guanidino acetic acid (GAA) levels with different protein sources (fishmeal and non-fishmeal) had a significant effect on total erythrocyte of tilapia, but did not have significant differences between treatments on total leukosyte and hematocrit. Treatment with 1.2 g/kg GAA, fish meal protein source was the best treatment in this research.
Pemberdayaan Pembudidaya Ikan Desa Pasirian melalui Pelatihan Produksi Probiotik dan Pakan Fermentasi untuk Kemandirian Pangan Muhammad Fakhri; I Made Dedi Mahariawan; Wahyu Endra Kusuma; Nasrullah Bai Arifin; mfakhri@ub.ac.id
Sewagati Vol 9 No 5 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i5.9046

Abstract

Pembudidaya ikan di Desa Pasirian menghadapi kendala meningkatknya biaya pakandan rendahnya kelulushidupan ikan. Program pengabdian kepada masyarakat inibertujuan untuk memperkuat kemandirian pangan bagi pembudidaya ikan di DesaPasirian melalui pengenalan teknologi produksi probiotik dan pakan fermentasi.Metode pelaksanaan dilakukan melalui survei lapangan, sosialisasi dengan ceramahinteraktif, evaluasi pre-test dan post-test, pelatihan teknis berbasis praktik langsung,serta pendampingan selama satu bulan. Peserta mempelajari proses pembuatan probiotikberbahan lokal dan menerapkan teknik fermentasi dalam produksi pakan.Hasil evaluasi menunjukkan peningkatan rata-rata pengetahuan peserta dari 30%pada pre-test menjadi 85% pada post-test, sedangkan keterampilan teknis meningkatdari 15% menjadi 70%. Temuan ini membuktikan adanya peningkatan signifikandalam pemahaman akan pentingnya pakan mandiri dan kemampuan praktik fermentasisecara mandiri. Program ini mengurangi ketergantungan pada pakan komersial,membangun model pemberdayaan partisipatif, serta mendorong inovasi lokal. Simpulanprogram menegaskan bahwa pelatihan berbasis masyarakat dengan dukunganpendampingan efektif dalam meningkatkan kapasitas teknis, mendorong praktikbudidaya berkelanjutan, serta mendukung ketahanan pangan lokal.
Application of wheat flour (Triticum aestivum) on spore density and sporulation efficiency of Bacillus megaterium isolated from Litopenaeus vannamei gastrointestinal tract I MADE DEDI MAHARIAWAN; WAHYU ENDRA KUSUMA; ATING YUNIARTI; M.A.G. BELTRAN; ANIK MARTINAH HARIATI
Biodiversitas Journal of Biological Diversity Vol. 22 No. 9 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220914

Abstract

Abstract. Mahariawan IMD, Kusuma WE, Yuniarti A, Beltran MAG, Hariati AM. 2021. Application of wheat flour (Triticum aestivum) on spore density and sporulation efficiency of Bacillus megaterium isolated from Litopenaeus vannamei gastrointestinal tract. Biodiversitas 22: 3709-3715. Bacillus megaterium is frequently used in fish farming, such as white shrimp (Litopenaeus vannamei) pond, which can produce spores with high stability in its implementation. Currently, spore production still requires the availability of high-cost carbon sources. The objective of this research was to evaluate the effect of different wheat flour doses on spore density and sporulation efficiency of B. megaterium BM1. In flasks, 50 mL of each test medium was treated with different doses of wheat (10, 20, 30 and 40 g. L-1, respectively) and glucose was used as a control. Each treatment was inoculated with B. megaterium BM1 (2.6 x 108 CFU. mL-1) and incubated in a shaker incubator (120 rpm) at 37 °C for 120 hours. The results showed that the highest vegetative cell concentration (17 x 108 CFU. mL-1), growth rate (0.8 hour-1) and spore (14.7 x 108 spores. mL-1) were found in the wheat flour dose of 30 g. L-1. Furthermore, the highest sporulation efficiency was achieved at 20 g. L-1 of wheat (91.30%) and germination should be done at a dose less than 40 g. L-1. The size of the spores was 1.35-1.39 µm. Thus, 30 g. L-1 of wheat flour is a potential dose to produce spore for probiotic candidates.  
POTENSI EKSTRAK TINTA GURITA SEBAGAI IMUNOSTIMULAN ANTI-BIOFILM STREPTOCOCCUS AGALACTIAE PADA AKUAKULTUR: Potential of Octopus Ink Extract as an Anti-Biofilm Immunostimulant for Streptococcus agalactiae in Aquaculture Rangga Idris Affandi; Bagus Dwi Hari Setyono; I Made Dedi Mahariawan; Jefri Anjaini; Feri Setiawan
Ganec Swara Vol. 20 No. 2 (2026): Juni 2026
Publisher : Yayasan Al-Amin Qalbu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59896/gara.v20i2.639

Abstract

Streptococcus agalactiae is a pathogenic bacteria in aquaculture, causing streptococcosis in fish and causing significant economic losses. The pathogenicity of this bacteria is related to its virulence factors, including its ability to form biofilms that increase antibiotic resistance and host immune responses. One alternative approach to controlling infection is to disrupt the bacterial quorum sensing (QS) communication system through a quorum quenching mechanism. This study aims to analyze the potential of octopus ink extract as a quorum quenching agent in inhibiting S. agalactiae biofilm formation in aquaculture. The method used was a systematic literature review by collecting and analyzing various scientific sources such as journals, books, and relevant articles. Data analysis was carried out using content analysis techniques by selecting the literature most relevant to the research topic. The results of the study indicate that octopus ink contains bioactive compounds, especially alkaloids, which have the potential to inhibit the QS system, suppress the expression of virulence factors, and inhibit bacterial biofilm formation. In addition, octopus ink extract also has the potential to improve the immune system of farmed fish. Thus, octopus ink extract has the potential to be developed as an environmentally friendly antibiofilm and immunostimulant agent. Further research is needed to identify specific active compounds and test their effectiveness and safety on a cultivation scale.
Co-Authors ANIK MARTINAH HARIATI Anik Martinah Hariati ATING YUNIARTI Ating Yuniarti Ating Yuniarti2 Bagus Dwi Hari Setyono Bagus R. Hidayat Denanta, I Made Kresna Fakhirah Oktafiani Afifah Feri Setiawan Gigih Setia Wibawa Hariati, Anik Martinah Heny Suprastyani, Heny Jefri Anjaini Kurniawan, Hendra Agung Lintang Dion Pangestu M.A.G. BELTRAN Mega Fatimah Rosana mfakhri@ub.ac.id Muhammad Dailami, Muhammad Muhammad Fakhri Nasrullah Bai Arifin Nugroho, Nurul Oktaviani, Elsa Dian Permatasari, Felyta Dian Putri, Ajeng Ayu Restuaning Rahardjo, Seto Sugianto Prabowo Rangga Idris Affandi Salsabila, Sivana Yesica Ayu Satria, Ibnu Darma Sa’adati, Feni Tri Sri Andayani Suciati, Lusia Dyah WAHYU ENDRA KUSUMA Wahyu Endra Kusuma Wahyu Endra Kusuma Widyawati, Yuni