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All Journal JBIO: jurnal biosains (the journal of biosciences) Syntax Literate: Jurnal Ilmiah Indonesia Makara Journal of Science
Rahadianto, Rahadianto
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Other

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Potential Effect of Phlorotannins in Brown Algae for Antidiabetic Therapy: Molecular Docking Approach Renagupita, Melani Sinastri; Rahadianto, Rahadianto; Garianto, Efyluk; Setianingsih, Herin
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to manifest the effect of the active compound phlorotannins in brown algae on decreasing insulin resistance by analyzing the predicted interaction between phlorotannins and protein tyrosine phosphatase 1B (PTP 1B) and estimating the pharmacokinetics and toxicity of the active compound for type 2 diabetes mellitus (DM) therapy. This type of research uses an in silico study to test the effect of using phlorotannins as an active compound in brown algae against PTP 1B inhibition. Starting from preparing materials, i.e., downloading the three-dimensional structure of phlorotannins via PubChem and PTP 1B via RSCB PDB (PDB 1A5Y), molecular docking using Molegro Virtual Docker 5, molecular visualization using PyMol and Discovery Studio, and predicting pharmacokinetics and toxicity via pkCSM have been conducted. Here, the phlorotannins include phloroglucinol, dioxinodehydroeckol, eckol, phlorofucofuroeckol-A, dieckol, 7-phloroeckol, and 6,6′-bieckol. In addition, Ertiprotafib and S-phosphocysteine are used here as the comparison controls for docking validation. All phlorotannins can bind to PTP 1B at the same binding site with drug control. Experimental results revealed that phlorotannins–PTP 1B produces lower energy than complex S-phosphocysteine–PTP 1B (−266.8 kJ/mol), which acts as a control here. However, phloroglucinol–PTP 1B produces (−208 kJ/mol) under the same condition. Compared with the drug control Ertiprotafib (−322.8 kJ/mol), the lower bond energy is owned by phlorofucofuroeckol-A (−370.6 kJ/mol), 7-phloroeckol (−328 kJ/mol), dieckol (−331.8 kJ/mol), and 6.6'-bieckol (−341 kJ/mol). Furthermore, phlorotannins are very well absorbed in the intestine. According to Lipinski’s rule, active compounds, such as phloroglucinol, eckol, and dioxinodehydroeckol have high potential as a drug. Phlorotannins are nontoxic against hepatocytes and have fewer side effects than drug control. Based on the obtained data, use of the active compound phlorotannins in brown algae can decrease insulin resistance, which can be employed as adjunctive therapy in type 2 DM.
Effects of grey mangrove leaf extract (Avicennia marina) on the growth of Staphylococcus aureus Priambodo, Bima; Budiarti, Retno; Rahadianto, Rahadianto; Nefertiti, Eva
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43222

Abstract

The emergence and spread of methicillin-resistant Staphylococcus aureus in recent decades complicates the antibiotic therapy. The grey mangrove (Avicennia marina) has flavonoid, terpenoid, saponin, phenolic, tannin, and alkaloid chemical compounds that have antimicrobial activity, thus potentially inhibiting Staphylococcus aureus. This study aims to examine the effects of the grey mangrove (Avicennia marina) leaf extracts on the growth of Staphylococcus aureus. This research uses laboratory experimental design with disc diffusion method to test the inhibition of Staphylococcus aureus bacteria growth in Mueller-Hinton medium. There were 6 treatment groups, namely negative control of 0.2% DMSO,  positive control of amoxicillin 30 µg, Avicennia marina leaf extract at concentration of 25%, 50%, 75%, and 100%. The results of this research showed that Avicennia marina leaf extract produces inhibition zone of 7.06 mm, 8.51 mm, 10.07 mm, 13.29 mm at concentration of 25%, 50%, 75%, and 100%. Meanwhile, positive control produces inhibiton zone of 23.65 mm, and negative control has no inhibition zone. The statistical tests using one-way ANOVA resulted in a significance value of less than 0.05 (p<α). The conclusion of this study showed that the grey mangrove (Avicennia marina) leaf extract at 100% concentration is the most effective treatment group in inhibiting the growth of Staphylococcus aureus, although the inhibition was not much better when compared to the inhibition of the positive control group.
Pengaruh Pemberian Gel Chitosan Dari Ekstrak Kulit Udang Vannamei (Litopenaeus Vannamei) Terhadap Angiogenesis Pada Tikus Jantan (Rattus Norvegicus) Pramana, Anak Agung Ngurah Gede Anggra; Rahadianto, Rahadianto; Biutifasari, Verna; Saputra, I Gede Agus Rio
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i1.56203

Abstract

Luka merupakan keadaan hilang atau rusaknya sebagian jaringan tubuh yang dapat disebabkan oleh trauma benda tajam atau tumpul, perubahan suhu, zat kimia, ledakan, sengatan listrik, atau gigitan serangga. Menurut WHO pada tahun 2018, Luka bakar adalah masalah kesehatan masyarakat global, yang menyebabkan sekitar 180.000 kematian setiap tahunnya. Kulit udang vannamei diketahui mengandung chitosan yang berpotensi dapat meningkatkan penyembuhan luka bakar. Tujuan penelitian ini untuk mengetahui pengaruh pemberian gel chitosan dari ekstrak kulit udang selama 7 hari secara topikal terhadap angiogenesis pada tikus jantan galur Wistar dengan luka bakar derajat II. Desain penelitian ini yang dilakukan di Laboratorium Biokimia Fakultas Kedokteran Universitas Hang Tuah Surabaya. rancangan penelitian ini adalah Post Test Only Control Group Design. Sampel yang digunakan adalah 18 ekor tikus jantan galur Wistar yang dibagi menjadi dua kelompok ; kelompok pertama, luka hanya dibiarkan sebagai kelompok kontrol. Kelompok kedua diberi gel chitosan dari ekstrak kulit udang secara topikal sebagai kelompok perlakuan, kemudian hari ke-8 tikus diambil jaringan kulitnya kemudian dibuat preparat histopatologi di Universitas Hang Tuah Surabaya. Tahap terakhir penelitian, dilakukan perhitungan jumlah angiogenesis. Data yang diperoleh dianalisis menggunakan uji statistik t dua sampel bebas. Hasil rerata jumlah angiogenesis pada kelompok kontrol sebanyak 3,83, pada kelompok perlakuan 6,72. Uji statistika uji t dua sampel bebas didapatkan hasil sebesar 0.001 (<0.05). Penelitian menunjukan bahwa terdapat pengaruh pemberian gel chitosan dari ekstrak kulit udang vannamei (litopenaeus vannamei) terhadap angiogenesis pada tikus jantan (rattus norvegicus) galur wistar dengan luka bakar derajat II.
Effects of grey mangrove leaf extract (Avicennia marina) on the growth of Staphylococcus aureus Priambodo, Bima; Budiarti, Retno; Rahadianto, Rahadianto; Nefertiti, Eva
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.43222

Abstract

The emergence and spread of methicillin-resistant Staphylococcus aureus in recent decades complicates the antibiotic therapy. The grey mangrove (Avicennia marina) has flavonoid, terpenoid, saponin, phenolic, tannin, and alkaloid chemical compounds that have antimicrobial activity, thus potentially inhibiting Staphylococcus aureus. This study aims to examine the effects of the grey mangrove (Avicennia marina) leaf extracts on the growth of Staphylococcus aureus. This research uses laboratory experimental design with disc diffusion method to test the inhibition of Staphylococcus aureus bacteria growth in Mueller-Hinton medium. There were 6 treatment groups, namely negative control of 0.2% DMSO,  positive control of amoxicillin 30 µg, Avicennia marina leaf extract at concentration of 25%, 50%, 75%, and 100%. The results of this research showed that Avicennia marina leaf extract produces inhibition zone of 7.06 mm, 8.51 mm, 10.07 mm, 13.29 mm at concentration of 25%, 50%, 75%, and 100%. Meanwhile, positive control produces inhibiton zone of 23.65 mm, and negative control has no inhibition zone. The statistical tests using one-way ANOVA resulted in a significance value of less than 0.05 (p<α). The conclusion of this study showed that the grey mangrove (Avicennia marina) leaf extract at 100% concentration is the most effective treatment group in inhibiting the growth of Staphylococcus aureus, although the inhibition was not much better when compared to the inhibition of the positive control group.
Co-Authors Biutifasari, Verna Garianto, Efyluk Nefertiti, Eva Pramana, Anak Agung Ngurah Gede Anggra Priambodo, Bima Renagupita, Melani Sinastri Retno Budiarti Saputra, I Gede Agus Rio Setianingsih, Herin