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All Journal Journal of Marine Research Omni-Akuatika Media Akuakultur Jurnal Perikanan Pantura JARI : Jurnal Akuakultur Rawa Indonesia
Ahmad Beni Rouf
Program Studi Pendidikan Kelautan Dan Perikanan, Universitas Pendidikan Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Other

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Efficacy of GP-11 KHV DNA Vaccine in Common Carp (Cyprinus carpio) through Feed by Different Frequency of Administration Ahmad Beni Rouf; Sri Nuryati; Sukenda Sukenda; Alimuddin Alimuddin
Journal Omni-Akuatika Vol 16, No 1 (2020): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.1.768

Abstract

GP-11 KHV DNA vaccine is a vaccine that can be used to induce immunity against the KHV virus (Koi herpesvirus). Vaccination through feed is an alternative way of administering vaccines. The study aimed to examine the effect of giving KHV GP-11 DNA vaccine through feed with different frequencies to KHV infection. The frequency of vaccine administration is GP-11 vaccination once a week; GP-11(1x), GP-11 vaccination twice a week; GP-11(2x), GP-11 vaccination three times a week; GP-11(3x), GP-25 vaccinations three times a week; GP-25(3x), negative control (without KHV test) and positive control (KHV tested). The fish were kept for 28 days after vaccination and then continued with the KHV challenge test for 28 days. The weight of carp ranges from 13.82±2.37 g maintained with a density of 15 fish/aquarium. The results showed that vaccine treatment was able to induce an immune response as indicated by the number of white blood cells, lysozyme activity and post-vaccination antibody titer showed a significant effect compared to controls. Likewise, after the challenge test, supported by IFNγ and IgM gene expression parameters after the challenge test showed the highest value of vaccine treatment rather than control. The efficacy of vaccine was showed by RPS value (%) in each vaccine treatment obtained GP-11(1x) value of 44.7±3.7a, GP-11(2x) of 78.9±18.2b, GP-11(3x) 85.6±12.6b and GP-25(3x) 79.5±18.1b. It was concluded that administering the GP-11 vaccine frequency 2 times a week provides protection as strong as giving a vaccine frequency 3 times a week.Keywords: common carp, DNA vaccine, frequency of administration, koi herpesvirus
PENAMBAHAN FERMENTASI DAUN LAMTORO (Leucaena leucocephala) PADA PAKAN UNTUK PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN LELE SANGKURIANG (Clarias gariepinus) Yasna Hoerunnisa; Mad Rudi; Ahmad Beni Rouf
Jurnal Perikanan Pantura (JPP) Vol 7 No 2 (2024): September 2024
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/jpp.v7i2.8339

Abstract

Lamtoro leaves (Leucaena leucocephala) are used as feed ingredients because they contain a lot of protein but are hampered by the high fiber content but can be reduced by fermentation to cover the smell of lamtoro leaves which fish do not like. This research aims to examine the effect of fermenting lamtoro leaves into artificial feed for sangkuriang catfish (C.gariepinus) on the growth and survival of sangkuriang catfish (Clarias gariepinus). This research used 120 Sangkuriang catfish with ± 3 grams and a length of ± 5-6 cm. This research method uses a quantitative and experimental approach using a Completely Randomized Design (CRD) consisting of four and three replications consisting of control, 10% lamtoro leaf fermentation, 15% lamtoro leaf fermentation, and 20% lamtoro leaf fermentation. The research observation parameters were absolute weight, absolute length, feed conversion, specific growth rate, and survival of sangkuriang catfish (Clarias gariepinus). The results showed that treatment with the addition of fermented lamtoro leaves had no significant effect (P>0.05) on the survival and growth of sangkuriang catfish. The conclusion from this research is that fermentation of lamtoro leaves has no significant effect on the growth and survival of sangkuriang catfish (Clarias gariepinus).
PENGARUH PENCAMPURAN TEPUNG SPIRULINA PADA IKAN MAS KOKI (Carassius auratus) TERHADAP KECERAHAN WARNA Rouf, Ahmad Beni; Alfiansyah, Dimas Arif; Rudi, Mad
Jurnal Perikanan Pantura (JPP) Vol 8 No 1 (2025): Jurnal Perikanan Pantura (JPP)
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/jpp.v8i1.9328

Abstract

Ikan mas koki memiliki ciri khas yaitu tubuh bulat dan warna cerah yang menarik perhatian para pecinta ikan hias. Warna cerah pada ikan mas koki dihasilkan oleh kromatofor pada kulit yang membutuhkan asupan nutrisi tertentu untuk mempertahankan intensitasnya. Tanpa pakan yang mengandung pigmen, warna ikan akan memudar, sehingga diperlukan suplemen tambahan untuk menjaga kecerahan warna. Salah satu suplemen yang terbukti efektif adalah tepung Spirulina platensis. Kandungan karotenoidnya, seperti xantophyll dan zeaxanthin, berperan dalam meningkatkan warna merah jingga pada ikan mas koki. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian tepung Spirulina platensis dalam pakan buatan terhadap kecerahan warna ikan mas koki serta menentukan dosis optimalnya. Penelitian dilaksanakan selama 30 hari menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan: dosis 0%/kg pakan (A), 1%/kg pakan (B), 3%/kg pakan (C), dan 5%/kg pakan (D), masing-masing dengan tiga kali ulangan. Hasil menunjukkan bahwa tepung Spirulina platensis secara signifikan meningkatkan kecerahan warna ikan mas koki (P<0,05). Perlakuan D dengan dosis 5%/kg pakan menghasilkan peningkatan kecerahan warna tertinggi, yakni 59,47%, dibanding perlakuan lainnya. Penambahan tepung Spirulina platensis tidak memengaruhi panjang mutlak, berat mutlak, maupun tingkat kelangsungan hidup ikan, sehingga aman digunakan. Suplemen ini efektif untuk meningkatkan estetika warna ikan mas koki tanpa efek samping negatif pada pertumbuhan atau kesehatan ikan.
PENGARUH BAKTERI Lactobacillus casei FREEZE DRY TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN MAS (Cyprinus carpio) Yudistira, Piero Eka; Karima, Silviyani Nurul; Rouf, Ahmad Beni; Rudi, Mad; Krishanti, Ni Putu Ratna Ayu
Media Akuakultur Vol 19, No 2 (2024): Desember 2024
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.19.2.2024.63-70

Abstract

Ikan mas (Cyprinus carpio) merupakan salah satu komoditas penting dalam budidaya perikanan air tawar. Kualitas pakan yang kurang optimal sering kali mempengaruhi pertumbuhan dan kelangsungan hidup ikan mas. Peningkatan pertumbuhan dan kelangsungan hidup ikan ini dapat didukung oleh penggunaan Lactobacillus casei probiotik freeze dry dalam pakan. Teknologi freeze dry memperpanjang penyimpanan probiotik tanpa mengurangi efektivitas dan menjaga viabilitasnya dalam pakan ikan. Penelitian ini bertujuan untuk mengevaluasi efektivitas Lactobacillus casei probiotik freeze dry terhadap pertumbuhan dan kelangsungan hidup ikan mas. Metode penelitian yang digunakan adalah eksperimental dengan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan dan tiga ulangan: PK1 (105 CFU/g), PK2 (106 CFU/g), PK3 (107 CFU/g), dan K (tanpa probiotik). Ikan mas yang digunakan berukuran 7-8 cm dengan padat tebar 10 ekor/20L air. Waktu pemeliharaan dilakukan selama 14 hari pada pemberian pakan dengan perlakuan dosis probiotik berbeda. Penambahan dosis probiotik L. casei pada pakan ikan mas menunjukkan peningkatan signifikan pada pertumbuhan dan kelangsungan hidup, dengan dosis optimal 0,1 gr/100 gr pakan (105 CFU/g), yang menghasilkan bobot mutlak (2,8 ± 0,12g), panjang mutlak (1,1 ± 0,21cm), specific growth rate (SGR) (1,7 ± 0,06%/hari), feed conversion ratio (FCR) (1,5 ± 0,06), efisiensi pemanfaatan pakan (EPP) (65,3 ± 2,27%), dan survival rate (SR) (100 ± 0%).The common carp (Cyprinus carpio) is an important commodity in freshwater aquaculture. Suboptimal feed quality often affects the growth and survival of common carp. Growth and survival of this species can be enhanced by incorporating freeze-dried probiotic bacteria into their feed. Freeze-drying technology extends the storage of probiotics without reducing their effectiveness, while maintaining viability in fish feed. This study aims to evaluate the effectiveness of freeze-dried probiotic bacteria on the growth and survival of common carp. The experimental method used was a completely randomized design (CRD) with four treatments and three replicates: PK1 (0.1 g probiotics/100 g feed, 10u CFU/g), PK2 (1 g probiotics/100 g feed, 10v CFU/g), PK3 (10 g probiotics/100 g feed, 10w CFU/g), and K (no probiotics). The common carp used were 7-8 cm in size with a stocking density of 10 fish/20 L of water. The feeding period lasted 14 days with probiotic doses applied. Adding Lactobacillus casei probiotic to the feed of carp significantly improved growth and survival, with the optimal dosage of 0.1 g/100 g of feed (105 CFU/g), resulting in absolute weight (2.8 ± 0.12g), absolute length (1.1 ± 0.21cm), specific growth rate (SGR) (1.7 ± 0.06%/day), feed conversion ratio (FCR) (1.5 ± 0.06), feed utilization efficiency (EPP) (65.3 ± 2.27%), and survival rate (SR) (100 ± 0%)
PERENDAMAN BURAYAK IKAN CUPANG (Betta sp.) MENGGUNAKAN MADU TIKUNG UNTUK MENINGKATKAN PERSENTASE JUMLAH JANTAN Anggoro, Aldio Purbo; Rudi, Mad; Prasetiyo, Himawan; Rouf, Ahmad Beni
JARI : Jurnal Akuakultur Rawa Indonesia Vol. 12 No. 1 (2024): JARI : JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v12i1.5

Abstract

Male betta fish (Betta sp.) have a high commercial value due to their superior aesthetic qualities compared to females. Sex reversal applications to produce male populations would provide higher profits for ornamental fish breeders. Synthetic steroid hormones are commonly used for sex reversal to induce masculinization. However, synthetic hormones have been banned in aquaculture due to their negative environmental impacts. Therefore, alternative natural and environmentally friendly substances, such as honey, are needed. This study aimed to immerse betta fish fry (Betta sp.) in tikung honey to enhance the percentage of males. The research was conducted experimentally using a completely randomized design with five treatments (P0, P1, P2, P3, P4) and three replications each. The experiment involved immersing 5-day-old betta fry in tualang honey at predetermined doses for each treatment. Treatment P0 served as the control group without honey immersion, while treatments P1, P2, P3, and P4 involved tikung honey immersion at doses of 5, 7, 9, and 11 ml/L, respectively. The results showed that the immersion of betta fry in tikung honey significantly influenced the increase in the percentage of males (P<0,05). The post hoc LSD test revealed a significant difference (P<0,05) between the control group (P0) and all other treatments (P1, P2, P3, and P4). The highest percentage of males was observed in treatment P2 (67.88±10.65%). Based on the findings of this study, the immersion of betta fish fry in tikung honey can effectively increase the percentage of males.
Kandungan Logam Berat Pada Ikan Pelagis di Pesisir Kota Cilegon Silvi Olivia Putra Nia; Agung Setyo Sasongko; Ahmad Beni Rouf
Journal of Marine Research Vol 14, No 2 (2025): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v14i2.44745

Abstract

Penelitian ini dilakukan dengan tujuan untuk mengetahui kandungan logam berat tembaga (Cu) dan juga kadmium (Cd) pencemaran pada perairan muara sungai sekitar wilayah Kota Cilegon. Stasiun penelitian ditentukan dengan menggunakan metode purposive sampling. Penelitian yang dilakukan merupakan penelitian deskriptif kuantitatif dengan menggunakan data primer dari observasi. Penelitian ini juga akan mengambil sampel organisme yaitu ikan pelagis yang ada di sekitar muara sungai. Selanjutnya dilakukan analisis logam berat menggunakan Atomic Absorption Spectrophotometry (AAS) untuk mengetahui kandungan logam pada organisme. Hasil penelitian sesuai dengan Peraturan Pemerintah No. 22 Tahun 2021 tentang Penyelenggaraan Perlindungan dan Pengelolaan Lingkungan Hidup menunjukkan perairan muara sungai di wilayah Kota Cilegon sebagian tidak terdeteksi adanya kandungan logam berat Kadmium (Cd), namun sebagian lagi terdeteksi adanya kandungan logam tembaga (Cu), sebesar 0,116 mg/L. Demikian pula organisme yang hidup di dalam perairan, yaitu ikan. Ikan pelagis kecil sering ditemui di berbagai muara sungai, ikan ini bersifat estuari (air tawar, dan air asin). Hasil pengamatan logam berat menunjukkan beberapa ikan (ikan belanak, ikan kaca asia, dan ikan mujair) yang sering ditemui mengandung logam berat kadmium (Cd) dan tembaga (Cu), dengan rata-rata logam kadmium (Cd) sebesar 1,2517 mg/kg dan rata-rata logam tembaga (Cu) sebesar 0,5527 mg/kg. Hasil ini tentu saja lebih dari batas standar baku mutu yang ada sesuai dengan Permen KP Nomor: 37/PERMEN-KP/2019 baku mutu tembaga (Cu) ialah 0,3 mg/kg dan sesuai dengan Peraturan Badan Pengawas Obat dan Makanan Nomor 9 Tahun 2022 baku mutu Kadmium (Cd) ialah 0,05 mg/kg, sehingga ikan-ikan di perairan ini tidak bisa dikonsumsi.  This study was conducted to determine the content of heavy metals, such as copper (Cu) and cadmium (Cd), that are polluting the estuary waters around the Cilegon City area. The research station was determined using the purposive sampling method. The research was conducted in a quantitative descriptive study using primary data from observations. This study will also take samples of organisms, namely pelagic fish, around the estuary. Furthermore, heavy metal analysis was carried out using Atomic Absorption Spectrophotometry (AAS) to determine the metal content in organisms. The results of the study following Government Regulation No. 22 of 2021 concerning the Implementation of Environmental Protection and Management showed that the waters of the river estuary in the Cilegon City area were partially undetectable for heavy metal Cadmium (Cd). Still, some were detected for copper (Cu), amounting to 0.116 mg / L. Similarly, the organisms living in the waters of the river estuary in the Cilegon City area were not detected. Similarly, the organisms that live in the waters are fish. Small pelagic fish are often found in various river estuaries; these fish are found in both freshwater and saltwater estuaries. The results of heavy metal observations show that some fish (mullet, Asian glassfish, and tilapia) are often found to contain heavy metals cadmium (Cd) and copper (Cu), with an average cadmium metal (Cd) of 1.2517 mg/kg and an average copper metal (Cu) of 0.5527 mg/kg. These results are of course more than the limit of the existing quality standards following KP Regulation Number: 37/PERMEN-KP/2019 copper (Cu) quality standard is 0.3 mg/kg and following the Food and Drug Administration Regulation Number 9 of 2022 the quality standard for Cadmium (Cd) is 0.05 mg/kg, so the fish in these waters cannot be consumed. 
Co-Authors Agung Setyo Sasongko Alfiansyah, Dimas Arif Alimuddin Alimuddin Anggoro, Aldio Purbo Karima, Silviyani Nurul Krishanti, Ni Putu Ratna Ayu Mad Rudi Prasetiyo, Himawan Rudi, Mad Silvi Olivia Putra Nia Sri Nuryati Sukenda Sukenda Yasna Hoerunnisa Yudistira, Piero Eka