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DNASe I and structure Catalytic site Nurul Sulviani
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.43429

Abstract

This Article describes 3Dimention studies carried out of catalytic site  over the past year which have advanced of understanding the substrate to recognizition and how the catalytic mechanism of the several key DNAse I. The hydrolysis reaction that occurs in DNASe I is influenced by Mg 2+ and Ca 2+ but let the Binding of DNASe I ions is still unclear, the active side is found, the amount and mechanism of Ca2+ and Mg2+ . The results of the review in this article indicate that DNASe I is needed externally from experimental animals and simulations improve the binding reaction of Ca 2+ and Mg 2+ which is supported by some literature that uses crystallographic studies where Ca 2+ ions can stabilize DNASe I and also in the presence of Mg 2+ ions that can accelerate the hydrolytic failure of the cation. The results of the review explain the role of cations collectively electrostatic matching between DNASe I and DNA.
Edukasi dan Konseling Kesehatan Mental di Kalangan Mahasiswa Teuku Muhammad Ilzana; Asyrafun Nisa Adelaidey; Irpan Efendi Rambe; Zanurul Rifhan; Vito Filbert Jayalie; Nurul Sulviani
Marsipature Hutanabe: Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 02 (2024): Marsipature Hutanabe: Jurnal Pengabdian Kepada Masyarakat
Publisher : CV. Devi Tara Innovations

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Abstract

This community service aims to increase students' awareness, understanding, and skills in maintaining mental health through education and counseling programs. Student life is often filled with academic, social, and adaptable pressures to a new environment that can trigger mental health problems such as stress, anxiety, and depression. The program includes seminars, workshops, and counseling sessions held at several colleges with a focus on introducing the importance of mental health, coping techniques, and appropriate psychological support. This activity involves students' active participation in discussions, role-plays, and simulations, which helps them better understand how to manage emotions and seek help when facing problems. The evaluation of the program showed an increase in students' knowledge and skills in dealing with mental health problems, as well as an increase in the number of students willing to access counseling services. Through this activity, it is hoped that a campus environment that is more supportive of mental health and reduces the stigma of seeking professional help.
The Relationship Between the Frequency of Independent Anatomy Study and the Anatomy Exam Results of Students of the Faculty of Medicine and Health, Imelda University, Medan Nurul Sulviani; Inawaty Inawaty; Indah Suhety; Febi Putri Lestari Hutasuhut; Zanurul Rifhan; Teuku muhammad ilzana; Asyrafun Nisa Adelaide; Ibnu arizona; Meldawati Meldawati
Benih : Journal of Midwifery Vol. 4 No. 01 (2025): Benih : Journal of Midwifery
Publisher : Cattleya Darmaya Fortuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54209/benih.v4i01.365

Abstract

This study aims to examine the relationship between the frequency of independent anatomy study and anatomy block exam results among undergraduate medical students. The data analysis revealed a positive and significant correlation between the two variables, with a Pearson correlation coefficient of r = 0.684, indicating a strong relationship. Furthermore, the simple linear regression analysis showed that each increase in the level of independent study frequency contributed to a 4.82-point increase in the anatomy block exam score. These findings suggest that the more frequently students engage in independent anatomy study, the more likely they are to achieve better academic outcomes in anatomy courses
The Relationship between Stress Levels and Cardiovascular Health Parameters in Students of the Faculty of Medicine, Imelda University, Medan Irpan Efendi Rambe; Ibnu Arizona Siregar; Nurul Sulviani; Inawaty; Meldawati
Benih : Journal of Midwifery Vol. 4 No. 02 (2025): Benih : Journal of Midwifery
Publisher : Cattleya Darmaya Fortuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54209/benih.v4i02.494

Abstract

Cardiovascular health is a critical aspect of overall well-being, particularly among medical students who often experience high levels of academic stress. Persistent stress has been shown to influence autonomic function, elevate blood pressure, and disrupt heart rate variability (HRV), thereby increasing the risk of early cardiovascular dysfunction. This study aims to examine the relationship between stress levels and cardiovascular health parameters—specifically blood pressure, resting heart rate, and body mass index (BMI)—among medical students at Universitas Imelda Medan. Using a cross-sectional design, data were collected through standardized stress questionnaires and physiological measurements. The findings are expected to demonstrate a significant association between higher stress levels and adverse cardiovascular indicators. Understanding this relationship may contribute to the development of preventive strategies and stress management interventions within medical education settings. Ultimately, this study highlights the importance of promoting mental well-being and healthy lifestyle practices to protect cardiovascular health in young adult populations.
Differences in Serum Triglyceride Levels Between Blood Samples Immediately Centrifuged and Those Allowed to Clot Before Centrifugation Mariska Vacsriani; Nurul Sulviani; Irpan Efendi Rambe; Roby Pahala Januario Gultom; Meriani Herlina; Noradina Noradina; Mutiara Siahaan; Hastrina Novasari
Benih : Journal of Midwifery Vol. 5 No. 02 (2026): Benih : Journal of Midwifery
Publisher : Cattleya Darmaya Fortuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54209/benih.v5i02.616

Abstract

The pre-analytical phase is a critical stage in clinical laboratory testing because inappropriate specimen handling may affect the accuracy of laboratory results, including serum triglyceride measurements. This study aimed to determine the differences in serum triglyceride levels between blood samples that were immediately centrifuged and those allowed to clot before centrifugation. This analytical study employed a cross-sectional paired-comparison design involving 31 respondents selected using purposive sampling. Venous blood samples from each respondent were subjected to two different pre-centrifugation treatments: immediate centrifugation and clotting for approximately 20 minutes before centrifugation. Serum triglyceride levels were determined using the enzymatic colorimetric glycerol phosphate oxidase–phenol aminophenazone (GPO-PAP) method with a photometer at a wavelength of 546 nm. Data were analyzed using descriptive statistics and the Wilcoxon Signed Rank Test at a significance level of 0.05. The results showed that the mean serum triglyceride level in blood samples that were immediately centrifuged was 142 mg/dL, whereas the mean level in blood samples allowed to clot before centrifugation was 156 mg/dL. Statistical analysis revealed a significant difference between the two treatments (p < 0.001), indicating that pre-centrifugation sample handling significantly influenced serum triglyceride measurements. It can be concluded that blood samples allowed to clot before centrifugation produced significantly higher serum triglyceride levels than samples that were immediately centrifuged. Therefore, adherence to standardized pre-analytical procedures is essential to ensure the accuracy, reliability, and consistency of serum triglyceride measurements in clinical laboratory practice.
Development of a Digital Quality Assurance Framework to Support Sustainable Accreditation in the Faculty of Medicine Astrida N Marpaung; Nurul Sulviani; Febi Putri Lestari Hutasuhut; Irpan Efendi Rambe; Inawaty Inawaty; Naswa Atika Riskian; Rayhan Azizan
Benih : Journal of Midwifery Vol. 5 No. 02 (2026): Benih : Journal of Midwifery
Publisher : Cattleya Darmaya Fortuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54209/benih.v5i02.630

Abstract

This study aims to develop and evaluate a Digital Quality Assurance (DQA) Framework to support continuous accreditation readiness at the Faculty of Medicine, Universitas Imelda Medan (FK UIM). The study employed a mixed-method approach, combining Design Science Research for framework prototyping with a descriptive correlational (cross-sectional) design using questionnaires administered to lecturers and administrative staff involved in quality assurance. The results show that the level of Digital Quality Assurance Framework implementation was predominantly in the moderate category (45.0%), while the level of continuous accreditation readiness was predominantly in the not-ready category (57.5%). The Chi-Square test indicated a significant relationship between framework implementation and accreditation readiness (p = 0.001), with Spearman correlation showing a strong positive relationship (rs = 0.612). The resulting framework prototype consists of five modules: digital document management, performance indicator dashboard, learning outcome monitoring, early-warning, and automated reporting.