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All Journal Indonesian Journal of Tropical and Infectious Disease Majalah Kedokteran Andalas Jurnal Kesmas dan Gizi (JKG) Sumatera Medical Journal Journal of Community Service and Empowerment Narra J Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI) Eduvest - Journal of Universal Studies
Evita Mayasari
Department Of Microbiology, Faculty Of Medicine, Universitas Sumatera Utara
Aerospace Engineering Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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PREVALENCE OF ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL SPECIMENS IN ADAM MALIK HOSPITAL Evita Mayasari; Cherry Siregar
Majalah Kedokteran Andalas Vol 37, No 1 (2014): Published in May 2014
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.994 KB) | DOI: 10.22338/mka.v37.i1.p1-7.2014

Abstract

AbstrakAcinetobacter baumannii merupakan spesies Acinetobacter spp. tersering diisolasi darimanusia, dan lebih sering dijumpai pada infeksi nosokomial dibandingkan dengan infeksi dikomunitas. Eksistensi bakteri ini di lingkungan terkait dengan keragaman reservoir, kemampuanmemperoleh gen pembawa sifat resisten antimikroba, dan sifat resisten terhadap pengeringan.Infeksi disebabkan strain A.baumannii yang resisten terhadap banyak antibiotik tidak mudahdikendalikan dan menjadi permasalahan di berbagai negara. Penelitian ini bertujuan untukmengetahui prevalensi A.baumannii dari spesimen klinis di instalasi mikrobiologi klinik RSUPHaji Adam Malik serta pola kepekaannya terhadap berbagai antibiotik. Identifikasi dan ujikepekaan menggunakan mesin otomatis Vitek 2 dengan Advanced Expert System (AES).Penelitian ini menemukan 644/3693 (17,44%) isolat A.baumannii dari berbagai spesimen klinis.A.baumannii paling banyak diisolasi dari spesimen dahak. Penelitian ini menemukan 147/644(23%) bahwa isolat carbapenem-resistent A.baumannii (imipenem dan meropenem). Sebagianbesar isolat sensitif terhadap colistin, amikacin dan tigecycline. Prevalensi A.baumanni yangditemukan pada penelitian ini adalah rendah namun resistensinya tinggi terhadap antibiotikterutama golongan penicillin, cephalosporin dan fluoroquinolon.AbstractAcinetobacter baumannii is the most frequent species of Acinetobacter spp. isolated fromhumans and more common in nosocomial infection than it is in community acquired infection.A.baumannii existence in environment is associated with the diversity of its reservoirs, itscapacity to accumulate genes of antimicrobial resistence, and its resistence to desiccation.Infection of Multidrug resistent (MDR) strain of A.baumannii is not easy to manage and it hasbecome a problem in many countries. The aim of this retrospective study was to investigatethe prevalence of A.baumannii from routine clinical specimens sent to clinical microbiologylaboratory RSUP HAM Medan and its susceptibility pattern to various antibiotics. Identificationand susceptibility testing of A. baumannii was performed by Vitek 2 with Advanced ExpertSystem (AES). A total of 644/3693 (17.44%) A.baumannii isolates were identified from variousclinical specimens. From those isolates, there were 147 (23%) isolates of carbapenemresistentA.baumannii (imipenem and meropenem). A.baumannii mainly isolated from sputumspecimens, and most isolates were highly sensitive to colistin, amikacin and tigecycline.Low prevalence of A.baumannii was found in this study. However, the isolates showed highresistence level to antibiotics, particularly penicillin, cephalosporin and fluoroquinolones.
Improving knowledge and attitude to tackle COVID-19 transmission in a health care facility of North Sumatra’s village Evita Mayasari; Lokot D. Lubis; Nuraiza Meutia; Cut A. Adella; Eka R. Megawati
Journal of Community Service and Empowerment Vol. 4 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v4i2.25205

Abstract

COVID-19 hits Indonesia with massive social restrictions in many aspects of life. In some Indonesian rural areas, information related to the pandemic through online platforms and social media is accessible. However, most of the information is inaccurate. Improving individual knowledge is crucial to support the government’s campaign for COVID-19 prevention. This cross-sectional, pretest-posttest study aims to evaluate the knowledge and attitude of health personnel toward COVID-19 prevention in a primary healthcare facility in a rural area of North Sumatra. All twenty-two health personnel who worked in the hospital during the study were asked to fill in a two-sectioned questionnaire. The first section contained questions related to knowledge, and the second section was related to attitude toward COVID-19. Following the educational activity, each health personnel filled in the same questionnaire again. We analyzed the data statistically using two-way ANOVA (p<0.05, 95% CI). There were increases in knowledge (p < 0.001) and attitude (p = 0.0002) of the health personnel after the educational activity. Educational activity improves health personnel’s knowledge and attitude regarding COVID-19 prevention.  
The Relationship between Knowledge and Attitude with Behavior of Preventing Leptospirosis in Healthcare Workers at the Binjai City Yolanda Hanalena; Evita Mayasari
Sumatera Medical Journal Vol. 5 No. 1 (2022): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/sumej.v5i1.7845

Abstract

Leptospirosis is an infectious disease caused by spirochete from the genus Leptospira, namely Leptospira interrogans which is pathogenic for both humans and animals. L. interrogans infection is often associated with flooding, where L. interrogans is carried from soil contaminated with animal urine reservoirs and settles in water. Leptospirosis is estimated to cause 1030000 cases and 58900 deaths annually worldwide. Until now, Leptospirosis is still a threat to public health because it causes Extraordinary Events (KLB) in several regions in Indonesia. Leptospirosis outbreaks are related to the presence of risk factors, namely the high population of rats as reservoirs of Leptospira spp., poor environmental sanitation, and the increasing number of flood areas in Indonesia. The purpose of this study was to determine the relationship between knowledge and attitude with the behavior of preventing Leptospirosis in healthcare workers at the Sub-District Health Center, Binjai Timur, Binjai City, against Leptospirosis. This research is an analytic study with a cross sectional design. The research was conducted using a questionnaire instrument of knowledge level about Leptospirosis, attitudes towards Leptospirosis, and preventive behavior towards Leptospirosis.
Mesenchymal stem cells for immune modulation in systemic lupus erythematosus: From bench research to clinical applications Ginting, Andi R.; Munir, Delfitri; Amin, Mustafa M.; Darlan, Dewi M.; Putra, Agung; Rusda, Muhammad; Mutiara, Erna; Mayasari, Evita; Rozi, Muhammad F.
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.994

Abstract

Systemic lupus erythematosus (SLE) is a prevalent autoimmune disease affecting multiple organ systems. Disease progression is inevitable as part of its natural course, necessitating aggressive therapeutic strategies, particularly with the use of immunosuppressants. Long-term use of steroids and other immunosuppressants is associated with significant adverse effects. Mesenchymal stem cells (MSCs) have been shown to modulate the immune response, leading to immunosuppressive effects against self-antigens. MSCs have demonstrated the ability to modulate several immune cell populations, contributing to favorable outcomes in controlling immune and inflammatory conditions. Recent evidence has shown an increase in Treg and Breg cell subsets following MSC administration, along with modulation of other immune cells, including dendritic cells, B cells, and T cells. However, the balance between MSC pro-inflammatory and anti-inflammatory phenotypic activation remains a critical factor in determining therapeutic outcomes. Various covariates also influence the efficacy of MSC therapy. The aim of this study was to provide a comprehensive overview of the utilization of mesenchymal stem cells (MSCs) in SLE treatment, leveraging their immunomodulatory and immunosuppressive capabilities. Understanding the fundamental preclinical effects of MSCs and recent findings from clinical studies may enhance the potential of MSC therapy in the management of SLE patients.
Antimicrobial Activity of the Ethanolic Extract of Durio zibethinus Rind against ESBL-producing Escherichia coli and Klebsiella pneumoniae : Antimicrobial Activity of the Ethanolic Extract of Durian Rind against ESBL-producing E. coli and K. pneumoniae Kannady, Johnson; Mayasari, Evita; Mardiani, Tengku Helvi; Hutagalung, Sunna Vyatra; Alona, Ivana; Simatupang, Maria Magdalena
Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI) Vol. 7 No. 1 (2025): Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Publisher : TALENTA Publisher, Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jetromi.v7i1.19027

Abstract

Background: Durio zibethinus (durian) contains secondary metabolites with antibacterial potency, such as alkaloids, flavonoids, saponins, tannins, and glycosides. Indonesia is one of the world’s largest producing countries of durian, yet the thorny part of the fruit rind often ends up as waste. Investigations of durian rind for its antimicrobial activity will maximize the benefits and prevent waste accumulation. Objective: In this experimental study, we analyzed the antimicrobial activity of ethanolic extract of durian rind against extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and K. pneumoniae in vitro. Methods: This study used a one-group posttest-only design. The disc diffusion method for antimicrobial susceptibility test was used to test the ethanolic extract of durian rind against ESBL-producing E. coli and K. pneumoniae, each with six experimental repetitions to observe the inhibition zone produced around discs containing the extracts at various concentrations (12.5%, 25%, 50%, 75%, 80%, and 100%). Results: Ethanolic extract of durian rind showed an inhibition zone against ESBL-producing E. coli at the extract concentration of 50%, with an average inhibition zone of 3.57 mm. The inhibition zone was also observed against ESBL-producing K. pneumoniae at the extract concentration of 75%, with an average inhibition zone of 3.73 mm. Conclusions: The ethanolic extract of durian rind showed inhibition zones against ESBL-producing E. coli and K. pneumoniae, showing its potential antimicrobial activity in vitro.
Direct PCR for Escherichia coli: a straightforward and cost-effective method Harahap, Desy Aryani; Yamamoto, Zulham; Widjaja, Sry Suryani; Mayasari, Evita
JURNAL KESMAS DAN GIZI (JKG) Vol. 7 No. 2 (2025): Jurnal Kesmas dan Gizi (JKG)
Publisher : Fakultas Kesehatan Masyarakat Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jkg.v7i2.2606

Abstract

This study compares the direct PCR method with standard PCR to detect Escherichia coli. Escherichia coli is the most widely used organism in biology. Detection of E. coli in water and food is routinely performed, utilizing methods like PCR. The initial stage of PCR preparation involves DNA extraction, which requires a commercial kit. Consequently, this extraction process incurs additional expenses, time, and labor. Therefore, an alternative method is needed, such as direct PCR, which can circumvent the need for DNA extraction. The PCR process facilitates lysis of the bacterial cell wall, releasing nucleic acids, which can then be amplified by Taq polymerase. For the PCR procedure, two groups were formed, each comprising three replicates of the reaction with different DNA templates. The first group utilized a direct culture of Escherichia coli, while the second group incorporated the extracted DNA of Escherichia coli. Our study successfully amplified the metH gene of Escherichia coli without DNA extraction. Electrophoresis analysis revealed that the direct PCR product, sized at 300 bp, appeared more pronounced than the standard PCR product. The findings of this research demonstrated direct PCR as an alternative for detecting Escherichia coli, which would lead to reductions in costs, time, and labor.
The Relationship Between Knowledge and the Utilization of the Maternal and Child Health (MCH) Book Among Pregnant Women at Tanjung Rejo Public Health Center, Deli Serdang Regency Hamonangan Sihombing, Rahmat Jody; Sukatendel, Khairani; Rangkuti, Deske Muhadi; Mayasari, Evita; Hasiana Siburian, Miranda Olivia
Eduvest - Journal of Universal Studies Vol. 5 No. 11 (2025): Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v5i11.52192

Abstract

Maternal Mortality (MMR) is still a global health problem that needs serious attention. In 2022, Deli Serdang Regency was recorded to have the highest AKI in North Sumatra Province, which was 16 per 42,363 live births. One of the efforts that can be made to reduce this number is by using the Maternal and Child Health Book (KIA). This study aims to analyze the relationship between the level of knowledge of pregnant women and the use of KIA books at the Tanjung Rejo Health Center, Deli Serdang Regency. This study uses an analytical observational design with a cross-sectional approach. A total of 100 pregnant women became respondents to the study. Data was collected through questionnaires, then analyzed using the Spearman statistical test. The use of KIA books is assessed through indicators of readability, comprehension, and application of information, while mother's knowledge is measured through the level of mastery of the content of KIA books. The results showed that the majority of pregnant women were at healthy reproductive age (55%), poorly educated (72%), unemployed (89%), and had high parity (56%). Most respondents (73%) fall into the category of poor utilization of KIA books, while 79% have a low level of knowledge. Statistical analysis showed a significant relationship between the level of knowledge of pregnant women and the use of KIA books (p = 0.001). The use of KIA books in pregnant women is significantly influenced by the level of knowledge.
Antibacterial Effects of Cranberry and Lime Extracts Against Uropathogenic Escherichia coli (UPEC). Nasution, Afrida Aryani; Mayasari, Evita; Simatupang, Maria Magdalena; Amelia, Sri; Alona, Ivana
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.64056

Abstract

Urinary tract infections (UTIs) continue to increase in the number of cases and death rates every year. This increase in the number of deaths is due to an increasing trend in UTI cases in elderly patients. In Indonesia, UTI is generally caused by the uropathogenic Escherichia coli (UPEC) strain. The existence of resistant bacterial infections also triggers increased mortality in UTI patients. Several types of herbs, such as cranberries, are often used traditionally to treat UTI symptoms. Another herb that is also known to have antibacterial effects is lime. This experimental study examined the in vitro antimicrobial effects of ethanol extract of cranberry (Vaccinium macrocarpon) and ethanol extract of lime (Citrus aurantifolia L.) against UPEC by the Kirby-Bauer disc diffusion method. This study used a clinical sample obtained from an outpatient diagnosed with UTI in the Prof. dr. Chairuddin P. Lubis Universitas Sumatera Utara Hospital in March 2023. The UPEC strain was isolated and identified using the Vitek 2 Compact automated system at a local university hospital. The antimicrobial susceptibility test results indicated that the ethanol extract of cranberry did not produce an inhibition zone at concentrations of 12.5%, 25%, 50%, 60%, 70%, and 96%. The ethanol extract of lime demonstrated inhibitory zones against UPEC, with a mean diameter of 12.01 mm at a concentration of 12.5% and a mean diameter of 15.62 mm at 96%. The cranberry extract did not demonstrate an inhibitory zone against UPEC in vitro, whereas the lime extract did.
Correlation between Physical Activity and Happiness: A Cross-Sectional Study in Medan Aufa Awalia Said; Megawati, Eka Roina; Evita Mayasari; Farhat
Sumatera Medical Journal Vol. 9 No. 1 (2026): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/sumej.v9i1.17213

Abstract

Introduction: Physical activity contributes to mental well-being by stimulating neurotransmitters associated with pleasure and positive mood. Understanding this relationship within specific populations is important for promoting community health. Objective: This study aimed to evaluate the correlation between physical activity and happiness among residents of Medan, Indonesia. Methods: This cross-sectional study was conducted in Medan from May to November 2021 and involved 260 participants. Physical activity was assessed using the International Physical Activity Questionnaire, while happiness and affect were measured using the Oxford Happiness Questionnaire and the Positive and Negative Affect Scale. Data were analyzed using Spearman’s rank correlation test. Results: Overall physical activity was positively correlated with happiness (r = 0.132; p = 0.034) and positive affect (r = 0.214; p = 0.001). Vigorous physical activity showed positive correlations with happiness (r = 0.185; p = 0.003) and positive affect (r = 0.244; p = 0.001). Walking was also positively correlated with happiness (r = 0.132; p = 0.034) and positive affect (r = 0.214; p = 0.001). Moderate physical activity was not correlated with happiness (r = –0.017; p > 0.05) or positive affect (r = 0.065; p > 0.05). No correlation was found between physical activity and negative affect. Conclusions: Physical activity is positively correlated with happiness among residents of Medan.
Co-Authors Agung Putra Alona, Ivana Amin, Mustafa M. Aufa Awalia Said Cherry Siregar Cut A. Adella Darlan, Dewi M. Delfitri Munir Eka R. Megawati Eka Roina Megawati, Eka Roina Erna Mutiara Farhat Ginting, Andi R. Hamonangan Sihombing, Rahmat Jody Harahap, Desy Aryani Hasiana Siburian, Miranda Olivia Hutagalung, Sunna Vyatra Kannady, Johnson Lokot D. Lubis Mardiani, Tengku Helvi Nasution, Afrida Aryani Nuraiza Meutia Rangkuti, Deske Muhadi Rozi, Muhammad F. Rusda, Muhammad Simatupang, Maria Magdalena Sri Amelia, Sri Sukatendel, Khairani Widjaja, Sry Suryani Yamamoto, Zulham Yolanda Hanalena