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All Journal Natural Science: Journal of Science and Technology Biocelebes KOVALEN: Jurnal Riset Kimia Jurnal Biologi Tropis

Wahyu Harso

Jurusan Biologi, Fakultas MIPA Universitas Tadulako

Author-ID : 717985

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology

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PENINGKATAN KEMAMPUAN TUMBUH TANAMAN JAGUNG (Zea mays L.) PADA KONDISI CEKAMAN KEKERINGAN OLEH JAMUR MIKORIZA ARBUSKULAR Agustinur Agustinur; Yusran Yusran; Wahyu Harso
Biocelebes Vol. 12 No. 3 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.486 KB)

Increasing maize production in Indonesia has been efforted regarding to fulfill the need of human and animal foods by utilizing the marginal land which has poor soil and is often dry. Application of Arbuscular Mycorrhizal Fungi as a biofertilizer in marginal land is a suitable alternative to overcome its problems. The aim of this research was to observe the effect of the different amounts of mycorrhizal fungal inoculum application on maize plant growth under different water regimes. Maize plants were inoculated by 0, 15, and 20 gram inoculum from Gigaspora margarita species and grown under soil water content at 60, 40 and 20% of soil fied capacity respectively. The results showed that application of mycorrhizal inoculum increased P absorption and hence plant growth under different water regimes. Plant inoculated by 20 gram inoculum had higher plant growth than plant inoculated by 10 gram inoculum particularly under soil water content at 60% of field capacity however the degree of fungal colonization was not significantly different.

KAJIAN AUTEKOLOGI HARAO Areca vestiaria Giseke PADA HUTAN DATARAN RENDAH DI KAWASAN TAMAN NASIONAL LORE LINDU (TNLL) SULAWESI TENGAH Risma Risma; Wahyu Harso; Ramadanil Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.852 KB)

Areca vestiaria Giseke is an endemic palm and the main component of tropical rain forest in Wallacea region. The study of autecology of A. vestiaria at the lowland forest has never been done. This study was aimed to observe the biotic and abiotic factors surround the habitat A. vestiaria.. The results indicated that there were a number of plants growing surrounding A. vestiaria, but the highest important Value Index at the level tree, sapling, pole and seedling was Polyalthia glauca Boerl. with the IVI 59.04%, Semecarpus forstenii Blume. (66.90%), Polyalthia glauca Boerl. (82.95%) and Arenga undulatifolia (32.92%), respectively. Ordo Hymenoptera and Lepidoptera insects found on A. vestiaria during the observation time. Soil at observed area had pH value of 6.9 while N, P and organic matter concentration in the soil were 0.40%, 4.46 mg/g and 7.22%, respectively. Areca vestiaria grew under light intensity of 1005.5 lux, humidity of 89.3% and average daily temperature of 24.7%.

KAJIAN ETNOBOTANI MASYARAKAT SUKU DAMPELAS DI DESA TALAGA KECAMATAN DAMPELAS KABUPATEN DONGGALA, SULAWESI TENGAH Satria Dhika Saputra; Wahyu Harso; Ramadanil Ramadanil
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research entitled “Ethnobotany Study of Dampelas Tribe Community in Talaga Village, Dampelas District of Donggala Regency, Central Sulawesi” and had been conducted from June to August 2016. This research aimed to obtain information about the type of plants and their utilization by Dampelas tribe community. Since the purpose of this research was to find out the type of plants and their utilization, this research employed a descriptive design that used quantitative approach with Equation Index of Cultural Significance (ICS). The equation used in this research was aimed at analysing the cultural importance of a plant. Based on the results obtained from the field, Dampelas tribe community in Talaga Villaga had been utilizing 82 species of plants for their daily life. The researcher dicovered that plants that have the highest ICS value were “Pae” (Oryza sativa L.) and “Aluku” (Cocos nucifera L.). Pae was used as a ceremony material, animal feed and cosmetics, while Aluku or coconut tree was used as handicrafts, building materials and materials for cultural ceremony. Both plants have the highest ICS value which is 104. It was also dicovered that plants that have the lowest ICS score, which was 6, were “Gamir” (Uncaria gambir Hunter Roxb) and “Jarak” (Jatropha Curcas L). Both plants were used as materials for cultural ceremony.

UJI DAYA HAMBAT EKSTRAK DAUN BANDOTAN (Ageratum conyzoides L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Melsi Mengkido; Orryani Lambui; Wahyu Harso
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (853.409 KB)

Ageratum conyzoides L. is commonly known as weed species however leaf from this plant is used as traditional medicine for wound and skin infection. Furthermore it is important to test A. conyzoides L. leaves extract can inhibit the growth of Staphylococcus aureus bacteria which is causing infection disease. The content of this leaf which can inhibit bacterial growth is also obeserved. The study was conducted by growing S. aureus bacteria on nutrient agar (NA) and then a number of leave extract from A. conyzoides leaves (7.5, 15.0, 35 and 50%) were injected to NA by well diffusion method. Amoxicillin 0,6% and DMSO 1% were also injected as positve control and negative control respectively. Inhibition zone was measured based on diameter formed. The results showed that increasing leaves extract increased inhibition of S. aureus growth however 50% leave extract inhibited S. aureus growth less than Amoxicilin 0,6%.A. conyzoides leaf can inhibit S. aureus growth may be caused by terpenoid, fenol, saponin and alkaloid on its content.

UJI DAYA HAMBAT EKSTRAK DAUN LALUMPA (Melastoma malabathricum L.) TERHADAP PERTUMBUHAN BAKTERI Vibrio cholerae dan Staphylococcus aureus Mutmainnah Mutmainnah; Wahyu Harso; Orryani Lambui
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Vibrio cholera and Staphylococcus aureus are a gram negative and a gram positive bacteria respectively. Both of them can cause diseaces in human. They have differences in their cell wall composition. Differences in both bacteria in the resistance to antibacterial compounds interesting to learn. The aim of this study was to observe inhibition of Melastoma malabathricum leave extract to the growth of V. Cholera and S. aureus. The study was conducted with Completely Randomized Design. The treatment was tested with 10%, 20%, 40%, 60%, 80% of leave extract concentration. Amoxicillin 2% and Na-CMC 1% were also treated as positif and negative control. Each treatments was repeated three times. Extract was obtained by maceration method. Extract was injected on bacterial growth medium by well diffusion method. The result showed that increasing extract concentration increased inhibition of growth to both bacteria. V. cholera tended to be more resistant than S. aureus.

PEMBERIAN DOSIS INOKULUM JAMUR MIKORIZA ABUSKULA (JMA) DAN PUPUK P YANG BERBEDA TERHADAP SERAPAN P DAN PERTUMBUHAN TANAMAN JAGUNG (Zea mays L.) Givani Oktaviana; Yusran Yusran; Wahyu Harso
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1281.091 KB)

The effectivity of Arbuscular Mycorrhizal (AM) Fungi to increase P absorption is not only affected by AM fungal inoculum dose but also affected by soil P concentration. The aim of this study was to observe the effect of AM fungus inoculum doses and appropriate P concentration added in soil to increase maize growth. The study was conducted with completely randomized design based on two factors i.e. dose of AM fungus inoculums (0, 10, 20 g/polybag) and different P fertilizer levels (0, 1,8, 0,37 and 0,74 g/polybag). Gigaspora margarita species was used as a fungus inoculum. A polybag contained 5 kg air-dried soil. The results showed that application of AM fungus inoculum increased plant growth in low P soil while it did not increase plant growth in high P soil. Increasing fungus inoculum dose did not increase plant growth since the rate of root colonization was not significantly different.

KOMUNITAS GULMA PADA PERKEBUNAN KAKAO (Theobroma cacao) DI DATARAN TINGGI DESA DONGI-DONGI DAN DATARAN RENDAH DESA SIDERA Yayang Istikana; Wahyu Harso; Ramadanil Pitopang
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Different elevation of lands can affect environmental conditions such as light intensity, temperature, and humidity. The environmental conditions will affect the growth and species of weeds. The aim of this study was to compare the diversity and species composition of weed comunities on highland and lowland of cacao plantation. The study was conducted by colecting the weed species from 25 plots with a size of 2 x 2 m. Every plot was placed by purposive sampling. The result showed that there were 26 species of weeds on highland while only 15 species of weeds were found on lowland. Weeds on highland was dominated by Ageratum conyzoides with important value index (IVI) of 62.07% while on lowland was dominated by Euphorbia hirta with IVI of 26.56%. The weeds community has a medium diversity index while the value of Similarity Index (IS) was low (4.87%) between weeds community on highland and lowland. It could be concluded that different environmental condition would affect the number and species of weeds.

Test Of The Effectiveness Of Root Alelopathic Extract (Cyperus rotundus L.) and Bandotan (Ageratum conyzoides L.) On The Growth Of Tomato Plants (Solanum lycopersicum L.) Dewi Arini; Wahyu Harso; Asri Pirade Paserang
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Cyperus rotundus L. and Ageratum conyzoides L. are commonly found in tomato field as weeds species. Weed compete with the crop for nutrient, water and light. In addition, weed released allelopathy that inhibited the growth of crop. The aim of this study was to compare the ability of allelopathy from C. rotundus L. and Ageratum conyzoides L. root extract to inhibit tomato plant growth. The study was conducted in Completey Randomized Design with two factors. The first factor was root extract from either C. rotundus L. and A. conyzoides L. as allelopathy. The second factor was concentration of root extract from both weeds (100, 300 and 500 g/ml). Each treatment combination was repeated four times. The results showed that A. conyzoides L. root extract had higher inhibition to growth of tomato plant than C. rotundus L. root extract. Increasing root extract concentration from both weeds increased inhibition of tomato plant growth.

PENINGKATAN PERTUMBUHAN TANAMAN JAGUNG ( Zea mays L.) MENGGUNAKAN JAMUR MIKORIZA ARBUSKULAR DARI JENIS YANG BERBEDA PADA KONDISI CEKAMAN AIR isna isna; Wahyu Harso; Yusran Yusran
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15081

Arbsucular mycorrhizal fungi promote plant growth by enhancing mineral uptake. Contribution degree of arbuscular mycorrhizal fungi to promote plant growth depend on species of plant-fungus association. The aim of this study was to compare the ability of three species of Glomus to promote maize plant growth. Maize plants were inoculated with 20 g inoculum of either Glomus deserticola, Glomus etunicatum, or Glomus clorum. Inoculum was soil containing spore, hyphae and infected root. Maize plants without addition inoculum were also used as a control. Water availability in the soil as growing medium was maintained on 40% field capacity. The results showed that addition of inoculum from three species of Glomus increased average of maize plant shoot dry weight although there was no statisticaly significant differences. Maize plant inoculated with G. clorum had higher shoot dry weight than maize plant inoculated either with G. etunicatum or G. deserticola while root colonization by G. clorum was lowest.

PROFIL TOKSIKOLOGIS EKSTRAK DAUN TUMBUHAN BAKA-BAKA (Hyptis capitata Jacq.) PADA HATI TIKUS PUTIH (Rattus norvegicus) Sitti Ayu; Wahyu Harso; Maghfirahtul Jannah
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15082

Hyptis capitata Jacq. plant has been using for traditional medicine. Utilization of medicinal plant must always consider to given dose because of toxic effect when too much medicine is taken. The aim of this study was to measure the liver damage in rats caused by H. capitata Jacq. leave extract. Either 300 (P1), 600 (P2) or 900 (P3) µg/kgBW H. capitata Jacq. leave extract was given orally to rats every 24 hours during 14 days. Zero point five ml ethanol 96% was given daily (K-) and without given anything (K+) was also conducted as a control. Both macroscopic and microscopic of liver damage were assessed. The result showed that rats given P3 treatment had the highest liver damage. The liver damage in rats was not statistically significant difference between P3 and K- treatments. The lowest liver damage was in rats given K+ treatment. There was no significant difference between P1 and P2 treatments on rats liver damage. Utilization of medicinal plant as traditional medicine should always be consider to doses.

Co-Authors Abi Gael Sidon Agustinur Agustinur Ajis Ajis Amalia Puji` Cahyani Andi Wirdani Pettalolo Asri Pirade Paserang Devan Prima Dewi Arini Endang Lestari Givani Oktaviana Gobel, Sri Amaliah isna isna Khairun Nisa Magfirahtul Jannah Melsi Mengkido Meryany Ananda Moh Iqbal Moh. Iqbal, Moh. Mustafid Rasyiid Mutmainnah Mutmainnah Orryani Lambui Paserang, Asri Prismawiryanti Prismawiryanti PUTI ANDALUSIA SARIGANDO Banilai Ramadanil Pitopang Riska Palesa Risma Risma Rudy, Sri Utami Satria Dhika Saputra Sitti Ayu Sri Rahayu Tagentju, Indra A. Umrah Umrah Umrah Umrah Wasilahturahman Yayang Istikana Yusran Yusran Yusran Yusran

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