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All Journal Natural Science: Journal of Science and Technology Biocelebes KOVALEN: Jurnal Riset Kimia Jurnal Biologi Tropis
Wahyu Harso
Jurusan Biologi, Fakultas MIPA Universitas Tadulako
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology

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Test Of The Effectiveness Of Root Alelopathic Extract (Cyperus rotundus L.) and Bandotan (Ageratum conyzoides L.) On The Growth Of Tomato Plants (Solanum lycopersicum L.) Dewi Arini; Wahyu Harso; Asri Pirade Paserang
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.796 KB) | DOI: 10.22487/bioceb.v13i3.14970

Abstract

Cyperus rotundus L. and Ageratum conyzoides L. are commonly found in tomato field as weeds species. Weed compete with the crop for nutrient, water and light. In addition, weed released allelopathy that inhibited the growth of crop. The aim of this study was to compare the ability of allelopathy from C. rotundus L. and Ageratum conyzoides L. root extract to inhibit tomato plant growth. The study was conducted in Completey Randomized Design with two factors. The first factor was root extract from either C. rotundus L. and A. conyzoides L. as allelopathy. The second factor was concentration of root extract from both weeds (100, 300 and 500 g/ml). Each treatment combination was repeated four times. The results showed that A. conyzoides L. root extract had higher inhibition to growth of tomato plant than C. rotundus L. root extract. Increasing root extract concentration from both weeds increased inhibition of tomato plant growth.
PENINGKATAN PERTUMBUHAN TANAMAN JAGUNG ( Zea mays L.) MENGGUNAKAN JAMUR MIKORIZA ARBUSKULAR DARI JENIS YANG BERBEDA PADA KONDISI CEKAMAN AIR isna isna; Wahyu Harso; Yusran Yusran
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15081

Abstract

Arbsucular mycorrhizal fungi promote plant growth by enhancing mineral uptake. Contribution degree of arbuscular mycorrhizal fungi to promote plant growth depend on species of plant-fungus association. The aim of this study was to compare the ability of three species of Glomus to promote maize plant growth. Maize plants were inoculated with 20 g inoculum of either Glomus deserticola, Glomus etunicatum, or Glomus clorum. Inoculum was soil containing spore, hyphae and infected root. Maize plants without addition inoculum were also used as a control. Water availability in the soil as growing medium was maintained on 40% field capacity. The results showed that addition of inoculum from three species of Glomus increased average of maize plant shoot dry weight although there was no statisticaly significant differences. Maize plant inoculated with G. clorum had higher shoot dry weight than maize plant inoculated either with G. etunicatum or G. deserticola while root colonization by G. clorum was lowest.
PROFIL TOKSIKOLOGIS EKSTRAK DAUN TUMBUHAN BAKA-BAKA (Hyptis capitata Jacq.) PADA HATI TIKUS PUTIH (Rattus norvegicus) Sitti Ayu; Wahyu Harso; Maghfirahtul Jannah
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15082

Abstract

Hyptis capitata Jacq. plant has been using for traditional medicine. Utilization of medicinal plant must always consider to given dose because of toxic effect when too much medicine is taken. The aim of this study was to measure the liver damage in rats caused by H. capitata Jacq. leave extract. Either 300 (P1), 600 (P2) or 900 (P3) µg/kgBW H. capitata Jacq. leave extract was given orally to rats every 24 hours during 14 days. Zero point five ml ethanol 96% was given daily (K-) and without given anything (K+) was also conducted as a control. Both macroscopic and microscopic of liver damage were assessed. The result showed that rats given P3 treatment had the highest liver damage. The liver damage in rats was not statistically significant difference between P3 and K- treatments. The lowest liver damage was in rats given K+ treatment. There was no significant difference between P1 and P2 treatments on rats liver damage. Utilization of medicinal plant as traditional medicine should always be consider to doses.
UJI KEEFEKTIFAN EKSTRAK ALELOPATI AKAR TEKI (Cyperus rotundus L.) DAN BANDOTAN (Ageratum conyzoides L.) TERHADAP PERTUMBUHAN SAWI HIJAU (Brassica rapa L.) Sri Rahayu; Asri Pirade Paserang; Wahyu Harso
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15083

Abstract

Weeds such as Cyperus rotundus L. dan Ageratum conyzoides L. release allelopatic compounds which can inhibit the growth of crops. The aim of this study was to compare the inhibition of allelopatic compounds contained C. rotundus L. and A. conyzoides root exctract to Brassica rapa L. growth. This study was conducted in a greenhouse and was based on Completely Randomized Design with two factors. The first factor was type of root extracts (extract from either C. rotundus root or A. conyzoides root) and the second factor was concentration of root extract. Root extract concentrations added to growth medium of B. rapa L. 10 days after planting were 0, 100, 300 and 500 g/L respectifely. The addition of root extract was repeated every week until crop harvested. The results showed that there was not significantly different between C. rotundus and A. conyzoides roots extract to inhibit B. rapa growth. Increassing of root extract concentrations from both roots increased inhibition of B. rapa growth.
PENGARUH INTENSITAS CAHAYA MATAHARI DANKETERSEDIAAN AIR TERHADAP PERTUMBUHANTANAMAN CABAI RAWIT (Capsicum frutescens L.) Ajis Ajis; Wahyu Harso
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15084

Abstract

Light intensity is one the most important factor to the growth of Capsicum frutescens L, however high light intensity can cause high evaporation which causes plant suffering from drought. The aim of this study was to determine the effect of different sunlight intensities and soilwater availabity to C. frutescens growth. The study was conducted by a completely randomized design method with two factors. The first factor was light entensity which consisted of 100 and 50% sunlight intensity (4287 and 2587 lux). The second factor was soil water avaibility which consisted of 75, 50 and 25% fied capacity. The results showed that plants grown at 100% light intensity had higher growth than plants grown at 50% light intensity on every soil water availability treatments. Soil water availability was not significantly affecting plant growth. It might be caused by plant received short period of sunlight.
KAJIAN AUTEKOLOGI Areca vestiaria Giseke PADA HUTAN PEGUNUNGAN BAWAH NGATA TORO KAWASAN TAMAN NASIONAL LORE LINDU SULAWESI TENGAH Andi Wirdani Pettalolo; Wahyu Harso; Ramadanil Pitopang
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15265

Abstract

The research entitled Autecolgical study of Areca vestaria in Sub-Montane Forest Lore Lindu National Park at Toro village area has been conducted from April to May 2016. The objective of the research was to know the biotics and abiotics factor arround the habitat of Areca vestiaria. The results showed that The highest important value index (IVI) of tree, sapling, pole and seedling species wereFicus ampelos (68.69%), Aphanaximis polistachy (40.01%), Ardisia lateriflora (31.73%) and Ardisia lateriflora (19.23%) respectively. The insects actively interacted with A. vestaria were black ant and bee. Based on soil analyzed indicated that pH of soil 6.6 while N, P and organic matter concentration were 0.26, 0.207 mg/g and 76.1% respectively. A. vestaria grew under light intensity of 540.4 lux, humidity of 76.1% and average daily temperature of 24.4%.
PERTUMBUHAN BAWANG MERAH (Allium cepa L.) YANG DIBERI PUPUK KOMPOS CAIR DAN JAMUR MIKORIZA ARBUSKULAR Riska Palesa; Wahyu Harso
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15267

Abstract

The application of liquid compost and of beneficial soil microorganism such as arbsucular mycorrhizal fungi can be used to replace chemical fertilizer application. The aim of this study was to investigate the growth of red onion (Allium cepa L.) plant fertilized by liquid compost and inoculated by arbuscular mycorrhizal fungi. This study was conducted based on a completely randomized design with two factors. The first factor was an addition of liquid compost dosages (0, 50, 100 and 200 ml/polybag). The second factor was an addition of AM fungal inoculum (with and without addition). The results showed that the growth of red onion plant was not significantly affected by the addition of liquid compost and AM fungal inoculum. However, the red onion plant fertilized by 200 ml liquid compost per polybag had the lowest shoot dry weight. The addition of AM fungal inoculum was not increasing plant growth because the quality of used inoculum was not good.
EFEKTIVITAS Glomus clorum TERHADAP PERTUMBUHAN TANAMAN CABAI RAWIT (Capsicum frutescens L.), TOMAT (Solanum lycopersicum L.) DAN TERUNG (Solanum melongena L.) Khairun Nisa; Yusran; Wahyu Harso
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15271

Abstract

Increasing plant growth by arbuscula mycorrhizal fungi depends on the fungus and plant spesies. The aim of this study was to determine the efectivity of fungal mycorrhizal fungus on the growth of cayenne pepper, tomato and eggplant which are Solanaceae family members. The study was conducted by Completely Randomized Design method.with two factors. The fisrt factor was species of experimental plant consisted of cayenne pepper (Capsicum frutescens L.), tomato (Solanum lycopersicum L.) and eggplant (Solanum melongena L.). The second factor was addition of Glomus clorum inokulum consisted of with and without inoculum addition. The result showed that there was no effect of inoculum addition on plant growth of three experiemal plants. The quality of inoculum used for this experiment was not good enough furthermore fungi could not germinate and colonize plant root.
PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN Amalia Puji` Cahyani; Asri Pirade Paserang; Meryany Ananda; Wahyu Harso
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15781

Abstract

Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.
FITOKIMIA, DAN AKTIFITAS ANTIBAKTERI DARI Etlingera sublimata Poulsen (ZINGIBERACEAE), TUMBUHAN ENDEMIK SULAWESI: Phytochemical and Antibacterial Activity of Etlingera sublimata Poulsen (Zingiberaceae), Endemic Plant to Sulawesi Ramadanil Pitopang; Endang Lestari; PUTI ANDALUSIA SARIGANDO Banilai; Wahyu Harso
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.15957

Abstract

The research entitled "Phytochemical Screening and antibacterial activity of Etlingera sublimata Poulsen (Zingiberaceae), an Endemic Sulawesi of Plants was conducted from January 2020 to June 2020, at the Plant Biosystematics Laboratory and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. Samples of E. sublimata were collected from the mountane forests of the Lore Lindu National Park (LLNP), arround Sedoa village, Lore Utara District, Poso Regency The objectives of the research were to analyze the secondary metabolite compounds in E. sublimata plants and to determine the antibacterial activity of E. sublimata leaves extract in againts Salmonella thypii bacteria. Plant samples were extracted by maceration methods with 96% ethanol as a solvent. Actibacterial activity was tested by using agar well diffusion methods. The experiment was designed by Completely Randomized Design (CRD) with seven different concentration of E. sublimata extract (15, 25, 50, 75 and 85%). Extract and standard drugs were prepared in double-distilated water using Nutrient Agar tubes. 2% Chloramphenicol as standard drugs was used as a positive control and sterile aquadest was used as a negative control. The inhibition zone of bacteria and yeast growth around the disk was measured after 18 to 24 h incubation at 37°C. The results showed that the stem contains alkaloids, flavonoids, terpenoids, tannins and saponins. The rhizome containts alkaloid, flavonoids, terpenoids, tannins and saponins, the flowers containts alkaloid, terpenoids and tannins, while the leaves containts tannin and saponin compounds. Leaves extract of E.sublimata has inhibition activity on the cell growth a pathogenic Salmonella thypi bacteria that the effective extract concentration was 50% with an average inhibition zone ​​2.67 mm.
Co-Authors Abi Gael Sidon Agustinur Agustinur Ajis Ajis Amalia Puji` Cahyani Andi Wirdani Pettalolo Asri Pirade Paserang Devan Prima Dewi Arini Endang Lestari Givani Oktaviana Gobel, Sri Amaliah isna isna Khairun Nisa Magfirahtul Jannah Melsi Mengkido Meryany Ananda Moh Iqbal Moh. Iqbal, Moh. Mustafid Rasyiid Mutmainnah Mutmainnah Orryani Lambui Paserang, Asri Prismawiryanti Prismawiryanti PUTI ANDALUSIA SARIGANDO Banilai Ramadanil Ramadanil Pitopang Ramadanil, Ramadanil Riska Palesa Risma Risma Rudy, Sri Utami Satria Dhika Saputra Sitti Ayu Sri Rahayu Tagentju, Indra A. Umrah Umrah Umrah Umrah Wasilahturahman Yayang Istikana Yusran Yusran Yusran Yusran