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All Journal Archives of The Medicine and Case Reports Scientific Journal of Pediatrics Tropis: Jurnal Riset Teknologi Laboratorium Medis
Ka’bah
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Detection of SARS-CoV-2 Genes in Stool Samples of COVID-19 Survivors Using the Real-Time Reverse Transcriptase Polymerase Chain Reaction Method Ka’bah; Nur Laela Alydrus; Arlitha Dekayana; Kasmuddin; Rinaldi Indra Duma’ari
Archives of The Medicine and Case Reports Vol. 5 No. 1 (2024): Archives of The Medicine and Case Reports
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/amcr.v5i1.453

Abstract

The spread of a pneumonia outbreak infected with the new coronavirus (COVID-19) from Wuhan, China, has spread globally. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the pathogen responsible for the pneumonic plague. The angiotensin conversion enzyme (ACE2) is the cellular receptor for SARS-CoV-2 entry. ACE2 is highly expressed in the epithelium of the stomach, duodenum and rectum. In infected patients, some patients infected with SARS-CoV-2 experience unusual symptoms, namely gastrointestinal symptoms, namely diarrhea, nausea and vomiting. This study aims to detect the presence of the SARS-CoV-2 gene in stool samples from COVID-19 survivors using the method real time RT-PCR as the gold standard for confirming COVID-19 with the RdRp and Helicase (ORF1b) target genes. There were 12 samples of COVID-19 survivors who met the inclusion criteria. The results showed that of the 12 samples, 2 samples were positive for SARS-CoV-2 RNA with Ct values of 32.79 and 36.86 which were detected on days 18 and 22 days after their respiratory tract tests were negative.
Prevalence of Enterotoxigenic Escherichia coli -LT Gene Expression in Pediatric Diarrhea Patients in Makassar City, Indonesia: A Cross-Sectional Study Alydrus, Nur Laela; Ka’bah; Kasmuddin; Prelly Loupatti
Archives of The Medicine and Case Reports Vol. 5 No. 4 (2024): Archives of The Medicine and Case Reports
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/amcr.v5i4.612

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a significant cause of diarrheal disease worldwide, particularly in children. The heat-labile toxin (LT) is a crucial virulence factor encoded by the LT gene. This study investigated the prevalence of ETEC LT gene expression in children with diarrhea in Makassar City, Indonesia. A cross-sectional study was conducted from January 2023 to December 2023. Rectal swabs were collected from children under five years of age presenting with diarrhea at outpatient clinics in Makassar. DNA was extracted, and the presence of the ETEC LT gene was detected using polymerase chain reaction (PCR). Demographic and clinical data were collected via questionnaires. The ETEC LT gene was detected in 81 (18%) children. The prevalence was significantly higher in children under one year of age (51%) compared to older age groups. ETEC LT positive children were more likely to experience vomiting and fever. ETEC expressing the LT gene contributes to a significant proportion of pediatric diarrhea cases in Makassar. The higher prevalence in younger children highlights the vulnerability of this age group. These findings emphasize the need for improved sanitation and hygiene practices to reduce ETEC transmission.
High Prevalence of Soil-Transmitted Helminths Without Concomitant Anemia: A Cross-Sectional Study of Children in an Indonesian Urban Waste Disposal Site Ka’bah; Risma Yuliana
Scientific Journal of Pediatrics Vol. 2 No. 2 (2024): Scientific Journal of Pediatrics
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjped.v1i2.96

Abstract

Introduction: Soil-transmitted helminth (STH) infections are a dominant cause of morbidity, particularly anemia, in pediatric populations in low-resource settings. Children residing in urban waste disposal sites ("Tempat Pembuangan Akhir" - TPA) represent a uniquely vulnerable, under-studied population. This study investigates the paradoxical relationship between STH infection, hematological status, and iron reserves in children living at the TPA Tamangapa, Makassar, Indonesia. Methods: A cross-sectional study was conducted involving 120 children aged 5-10 years. Sociodemographic and hygiene data were collected via a structured questionnaire. Stool samples were analyzed using the quantitative Kato-Katz technique (duplicate slides) to determine STH prevalence and infection intensity (Eggs Per Gram - EPG). Venous blood was analyzed for complete blood count (CBC) using an automated hematology analyzer and for iron status (serum ferritin, serum iron, TIBC) via ELISA and colorimetric assays. Statistical analysis included descriptive statistics, Chi-square tests, t-tests, and logistic regression to identify associations. Results: The overall prevalence of STH infection was 55.0% (66/120). Ascaris lumbricoides was the most common infection (25.0%), followed by Trichuris trichiura (15.0%) and co-infections (15.0%). The majority of infections (86.4%) were of light-to-moderate intensity. Overt anemia (Hb < 11.5 g/dL) was rare and its prevalence was not significantly different between infected (10.6%) and non-infected (9.3%, p=0.814) children. Mean hemoglobin levels were statistically indistinguishable (12.4 g/dL vs. 12.6 g/dL, p=0.082). However, STH-infected children demonstrated significantly depleted iron stores: mean serum ferritin was 28.5 ng/mL versus 47.2 ng/mL in non-infected children (p<0.001). Consequently, iron depletion (ferritin < 20 ng/mL) was epidemic in the infected group (43.9%) compared to the non-infected group (7.4%, p<0.001). Conclusion: In this high-risk population, STH prevalence is high but is not associated with overt anemia. This paradox is resolved by comprehensive analysis revealing a strong, significant association with depleted iron stores. This "hidden morbidity" of iron depletion, undetectable by simple hemoglobin screening, underscores the need for integrated WASH (Water, Sanitation, Hygiene) programs and more sophisticated iron-status screening in high-risk pediatric populations.
Identifikasi larva nyamuk di Kelurahan Bulu Cendea Kabupaten Pangkep Rahmadana, Nurul; Ka’bah; Sulfiani
Tropis: Jurnal Riset Teknologi Laboratorium Medis Vol 1 No 1 (2024): Tropis: Jurnal Riset Teknologi Laboratorium Medis
Publisher : Program Studi Teknologi Laboratorium Medis

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Abstract

Mosquitoes are abundant in nature almost everywhere and are detrimental to human life. This research aims to determine the types of mosquito larvae in Bulu Cendea Village, Pangkep Regency. This research was carried out by collecting mosquito larvae in 17 residential houses. Collection of larvae in water reservoirs in houses and yards in Bulu Cendea Village, Pangkep Regency. Furthermore, observations were made in the DIII Medical Laboratory Technology tropical infections laboratory in July 2019. The results of the research showed that from 17 residential houses in Bulu Cendea Village, Pangkep Regency, there were 110 Aedes agepty larvae, 94 Culex, 9 Anopheles, 2 Mansonia. The total number of mosquito larvae present in Bulu Cindea District, Pangkep Regency, there were 215 larvae.
The Influence of Personal Hygiene on the Prevalence of Soil-Transmitted Helminths (STH) in Children: A Case Series in a Remote Indigenous Community in Indonesia Nur Laela Alydrus; Ka’bah; Rugayyah Alyidrus; Risky Nurul Fadlila RN; Fika Andriani
Scientific Journal of Pediatrics Vol. 3 No. 1 (2025): Scientific Journal of Pediatrics
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjped.v2i2.172

Abstract

Introduction: Soil-transmitted helminths (STHs) are a major public health concern, especially in remote indigenous communities with limited access to clean water and sanitation. This study aimed to investigate the influence of personal hygiene practices on the prevalence of STH infections in children in the Ammatoa Kajang community of Bulukumba Regency, Indonesia. Methods: An observational case series was conducted among children aged 3-13 years in the Ammatoa Kajang community. Data on personal hygiene practices were collected through questionnaires and interviews, while stool samples were examined for STH infections using the Kato-Katz method. Data were analyzed using correlation tests to determine the relationship between personal hygiene and STH prevalence. Results: The prevalence of STH infection was 45%, with Ascaris lumbricoides being the most common (30%), followed by Trichuris trichiura (10%) and hookworm (5%). Poor personal hygiene practices, such as infrequent handwashing, not using soap after defecation, and not wearing footwear, were significantly associated with an increased risk of STH infection. Conclusion: Personal hygiene plays a crucial role in the prevalence of STH infections among children in the Ammatoa Kajang community. Health promotion programs focused on improving personal hygiene practices, along with improved sanitation facilities, are essential for reducing the burden of STH infections in this remote indigenous community.
Co-Authors Alydrus, Nur Laela Arlitha Dekayana Fika Andriani Kasmuddin Nur Laela Alydrus Prelly Loupatti Rahmadana, Nurul Rinaldi Indra Duma’ari Risky Nurul Fadlila RN Risma Yuliana Rugayyah Alyidrus Sulfiani