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All Journal MULTIPLE: Journal of Global and Multidisciplinary Scientific Journal of Pediatrics
Risky Nurul Fadlila RN
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Religion Humanities Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Deteksi Gen blaTEM Salmonella typhi sebagai Penanda Resistensi Ampisilin Pada Sampel Darah Pasien Demam Tipoid Nurfitri Arfani; Indas Wari Rahman; A. Meryam Susanti; Andi Maya Kesrianti; Hilda Suku; Risky Nurul Fadlila RN; Ayusti Dirga
MULTIPLE: Journal of Global and Multidisciplinary Vol. 2 No. 8 (2024): Agustus
Publisher : Institute of Educational, Research, and Community Service

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Abstract

Deman tifoid adalah penyakit yang menyerang saluran pencernaan yang disebabkan oleh Salmonella typhi, dengan penularan melalui jalur fekal oral pada makanan dan minuman yang terkontaminasi kemudian masuk ke tubuh. Penanganan demam tifoid biasanya menggunakan jenis antibiotik ampisilin, penisilin, amoksisilin, namun penggunaan antibiotik ini sudah banyak yang mengalami resistensi antibiotik. Salah satu antibiotik yang sering mengalami resistensi adalah ampisilin yang dikode oleh gen blaTEM (ꞵ-Lactamase Temoneira) pada Salmonella typhi. Penelitian ini bertujuan untuk mendeteksi gen blaTEM Salmonella typhi sebagai penanda resistensi ampisilin pada penderita demam tipoid. Jenis penelitian yang dilakukan adalah deskriptif kualitatif dengan pendekatan cross sectional study menggunakan sampel darah untuk mendeteksi spesies bakteri dan gen pengkode resistensi dengan metode Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan dari 20 sampel yang dideteksi Salmonella typhi, ditemukan satu sampel yang positif berdasarkan hasil visualisasi elektroforesis yaitu pada target pita 401 bp, kemudian sampel yang positif tersebut dilanjutkan deteksi gen dan ditemukan gen blaTEM pada target pita 717 bp. Sehingga dapat disimpulkan bahwa terdeteksi satu sampel (5%) gen blaTEM Salmonella typhi dari sampel darah penderita demam tifoid dengan metode PCR
The Influence of Personal Hygiene on the Prevalence of Soil-Transmitted Helminths (STH) in Children: A Case Series in a Remote Indigenous Community in Indonesia Nur Laela Alydrus; Ka’bah; Rugayyah Alyidrus; Risky Nurul Fadlila RN; Fika Andriani
Scientific Journal of Pediatrics Vol. 3 No. 1 (2025): Scientific Journal of Pediatrics
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjped.v2i2.172

Abstract

Introduction: Soil-transmitted helminths (STHs) are a major public health concern, especially in remote indigenous communities with limited access to clean water and sanitation. This study aimed to investigate the influence of personal hygiene practices on the prevalence of STH infections in children in the Ammatoa Kajang community of Bulukumba Regency, Indonesia. Methods: An observational case series was conducted among children aged 3-13 years in the Ammatoa Kajang community. Data on personal hygiene practices were collected through questionnaires and interviews, while stool samples were examined for STH infections using the Kato-Katz method. Data were analyzed using correlation tests to determine the relationship between personal hygiene and STH prevalence. Results: The prevalence of STH infection was 45%, with Ascaris lumbricoides being the most common (30%), followed by Trichuris trichiura (10%) and hookworm (5%). Poor personal hygiene practices, such as infrequent handwashing, not using soap after defecation, and not wearing footwear, were significantly associated with an increased risk of STH infection. Conclusion: Personal hygiene plays a crucial role in the prevalence of STH infections among children in the Ammatoa Kajang community. Health promotion programs focused on improving personal hygiene practices, along with improved sanitation facilities, are essential for reducing the burden of STH infections in this remote indigenous community.
Co-Authors A. Meryam Susanti Andi Maya Kesrianti Ayusti Dirga Fika Andriani Hilda Suku Indas Wari Rahman Ka’bah Nur Laela Alydrus Nurfitri Arfani Rugayyah Alyidrus