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All Journal E-Jurnal Medika Udayana Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Journal of Pharmacy Tiara Bunda
Ilmia, Nisrien
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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PENELUSURAN FRAKSI AKTIF BIJI BUAH KALANGKALA (Litsea angulata) SEBAGAI ANTIOKSIDAN DAN ANTIDIABETES Ilmia, Nisrien
E-Jurnal Medika Udayana Vol 13 No 5 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i05.P17

Abstract

Biji buah kalangkala (Litsea angulata) adalah tanaman yang sering digunakan dalam pengobatan tradisional Kalimantan dan telah dilaporkan mengandung senyawa fenolik dan flavonoid. Senyawa fenolik dan flavonoid merupakan senyawa yang berperan dalam aktivitas antioksidan dalam tanaman, selain itu fenolik diketahui mampu melindungi sel beta pankreas dan menjaga kadar insulin dalam tubuh. Tujuan penelitian ini adalah mengetahui aktivitas antioksidan dan antidiabetes dari fraksi n-heksan, etil asetat dan metanol ekstrak etanol biji buah kalangkala. Metode yang digunakan dalam penentuan aktivitas antioksidan adalah metode DPPH dengan pembanding vitamin C, sedangkan dalam uji aktivitas antidiabetes dilakukan secara in vitro menggunakan metode aktivitas enzim ?-glukosidase dengan pembanding akarbosa. Hasil dari penelitian ini, aktivitas antioksidan fraksi n-heksan, etil asetat dan metanol menghasilkan nilai IC50 msing-masing 52,05+1,33 ppm; 46,10+0,16 ppm; 32,84+1,51 ppm dan aktivitas ?-glukosidase n-heksan 42,67+10,53 U/L, etil asetat 59,91+4,35 U/L, dan metanol 6,84+12,86 U/L, berdasarkan hasil penelitian menunjukkan fraksi metanol memiliki aktivitas antioksidan dan antidiabetes tebaik
ANALISIS PENAMBATAN SENYAWA CHARANTIN DARI BUAH PARE (Momordica charantia) PADA GLUCOSE TRANSPORTER 3 (GLUT3) Ilmia, Nisrien
Journal of Pharmacy Tiara Bunda Vol. 5 No. 2 (2025): Vol. 5 No. 2 (2025): Journal of Pharmacy Tiara Bunda (JPTB)
Publisher : Politeknik Tiara Bunda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62619/jptb.v5i2.256

Abstract

Glucose transporters have an important role in the body to transport blood sugar to other organs. In patients with diabetes mellitus, the signaling of GLUT, especially class 1, is reduced because it is affected by impaired insulin secretion. Bitter gourd is thought to have an anti-diabetic effect by lowering blood pressure, with the main component in it being charantin. Purpose: This study aims to determine the activity of charantin as a blood sugar lowering agent by inducing GLUT3 through a compound anchoring test. Methods: The type of research in this study was a computer-based experimental study using an in silico test or molecular docking by comparing the charantin test ligand and the native ligand as a comparison in the form of Octyl Glucose Neopentyl Glycol. Conclusion: The results of the compound anchoring test show that the binding energy (ΔG) affinity of charantin for GLUT3 is -7.5 Kcal/mol, while for Octyl Glucose Neopentyl Glycol it is -6.15 Kcal/mol, and charantin has activity like Octyl Glucose Neopentyl Glycol with amino acid residues that bind to the active site of the GLUT3 protein, namely AGR228. This indicates that the compounds present in bitter gourd can be predicted to be used as therapy for diabetes mellitus patients.
Antioxidant Activity and Lipid Content of Aurantiochytrium sp. from Raja Ampat and Kepulauan Seribu Witasari, Hardi Astuti; Kintoko, Kintoko; Mahfudh, Nurkhasanah; Suhendra, Suhendra; Rangkuti, Ahmad Faizal; Ilmia, Nisrien; Santoso, Blegoh Iwan; Hutari, Andri
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 2 (2025): August 2025
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.1015

Abstract

Raja Ampat (Southwest Papua Province) and Kepulauan Seribu (Jakarta Raya Province) are part of Indonesia#39;s territory, which includes the mangrove forest area. This habitat supports a diverse array of animals, plants, and microalgae, including Aurantiochytrium sp. It has antioxidant activity related to lipid content. This study aims to identify the lipid content and the antioxidant effects of the microalgae Aurantiochytrium sp.’s extract isolated from the mangrove forest areas of Raja Ampat and Kepulauan Seribu. The extraction process was conducted using the Folch method, which involves a 2:1 (v/v) chloroform-methanol solvent ratio. The microalgae Aurantiochytrium sp. extract of Raja Ampat (MAERA) and microalgae Aurantiochytrium sp. extract of Kepulauan Seribu (MAEKS) were analyzed using GC-MS, antioxidant potential tested in silico by target proteins (5ZLG, 2A1X, 3VLN, and 1EEM) using Autodock Tools, and in vitro by DPPH and ABTS radicals and the carotenoid bleaching method. The results showed that total squalene and fatty acids in MAERA and MAEKS were at different levels. Squalene as a ligand had the lowest binding energy (-9.4, -6.6, -7.7, and -6.5) compared to the native ligand (-5.6, -4.4, -5.2, and -5.2) and ascorbic acid (-5.6, -4.8, -5.3, and -4.9) by in silico antioxidant activity. The in vitro antioxidant activities by DPPH, ABTS, and ß-caroteen bleaching were 103.00 ± 3.75, 216.39 ± 367, 214.62 ± 7,07 (MAERA); and 131.85 ± 23.48, 225.50 ± 3.36, 251.69 ± 5.39 (MAEKS) were not significantly different (pgt;0.05) by all methods. These findings showed that the Aurantiochytrium sp. strains Raja Ampat and Kepulauan Seribu contained diverse levels of fatty acids and squalene. In the in silico study, squalene was evaluated and in vitro, the MAERA and MAEKS extracts showed moderate antioxidant activity.
Co-Authors Hardi Astuti Witasari Hutari, Andri Kintoko Kintoko Nurkhasanah Mahfudh Rangkuti, Ahmad Faizal Santoso, Blegoh Iwan Suhendra Suhendra