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Screening for Resistance of Tomato Lines Against Tomato chlorosis crinivirus Wahyudin, Denih; Damayanti, Tri Asmira; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol 18 No 1 (2022): Januari 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.1.19-29

Abstract

Screening for Resistance of Tomato Lines Against Tomato chlorosis crinivirus Infection of Tomato chlorosis crinivirus (ToCV) has been reported involved in yellow disease of tomato. Recently, incidence of Crinivirus is increasing in tomato growing areas in West Java. Growing resistant tomato varieties will be effective for viral disease management, although finding resistance sources is quite a challenge. The aim of the study was to determine the resistance level of 12 tomato lines to ToCV infection. Fourteen days after transplanting, tomato lines were inoculated with ToCV using 10 viruliferous whiteflies (Trialeurodes vaporariorum). Observations were made on disease variables (incubation period, incidence and severity of disease, virus titer) and agronomic variables (plant height, number of leaves, number and weight of fruits). The average incubation period ranged from 9.4–13.5 days, and disease incidence ranged from 90.9–100%. Visual symptoms varied from mild to moderate chlorosis and leaf curling with disease severity scores ranging from 1.0–3.0. Virus titers were measured based on ELISA’s absorbance values, which ranged from 0.358 to 1.122. In general, ToCV infection inhibited plant growth and decreased leaf number, inhibited fruit weight and number i.e. 6.0–37.8%, 8.6–39.5%, 2.7–33.7%, and 7.0–25.5%, respectively. Based on disease assessement paramaters, responses of tomato lines were categorized as susceptible (BISILB#1029A, BISILB#22, and BISILB#724B), moderate resistant (BISILB#825B, BISILB#60D, BISIKC#402, BISIKC#96D, and BISILB#40I), and resistant (BISILB#1372ORA, BISILB#703A, BISILB#703B, and BISILB#724A). However, the resistance trait showed no correlation with tomato yield. Therefore, it is necessary to improve the trait of four resistant genotype so that is more adaptive to cultivation environmental factors and can be utilized as the parent of elite ToCV-resistant tomato variety.
The Effect of Volume and Inoculation Method on the Development of Bacterial Panicle Blight Disease in Rice Plant Kusdiana, Alchemi Putri Juliantika; Hidayat, Joni; Zulaiha, Siti; Wahyudin, Denih
Akta Agrosia Vol 25 No 1 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.25.1.17-23

Abstract

Bacterial panicle blight in rice plants caused by Burkholderia glumae. These bacteria interfere with the grain filling process so that the grain is not filled and can cause yield losses of up to 75% on pathogenic infested land. This study was conducted to determine the effect of B. glumaesuspension volume and inoculation method on the development of bacterial panicle blight. The research was conducted on vegetative and generative rice plants using a factorial completely randomized design with the volume of bacterial suspension as the first factor and the inoculation method as the second factor. The results showed that the higher the volume of the B. glumaesuspension inoculated, the higher the severity of bacterial panicle blight in rice plants. Both the inoculation and injection methods can cause the same disease severity. Besides, the severity of bacterial panicle blight in the generative phase is more severe than in the vegetative phase