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All Journal Research Journal of Life Science Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science Jurnal HPT (Hama Penyakit Tumbuhan)
Syib’li, Muhammad Akhid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Journal : Research Journal of Life Science

 1 
Antifungal activity of clove (Eugenia caryophyllata) extract against Fusarium oxysporum cutinase enzyme in Tomato (Solanum lycopersicum) from in vitro study Khairunnisa', Amalia; Syib’li, Muhammad Akhid; Abadi, Abdul Latief
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.3

Abstract

Fusarium oxysporum f.sp. lycopersici presents a notable threat to tomato plants due to the hydrolytic activity of its cutinase enzyme, which facilitates the penetration of plant root tissues. Thus, reducing cutinase activity through the application of cloves as inhibitors offers an environmentally friendly alternative to hazardous chemical fungicides, showing great promise in controlling Fusarium wilt disease, particularly by targeting the cutinase enzyme of Fusarium oxysporum. This study involved GC-MS analysis of clove extract, fungal growth and cutinase activity assay using rhodamine-b olive oil media. Hence, this study aims to reveal the potential and mechanism of clove extracts as inhibitors of cutinase enzymes to reduce the penetration of the Fusarium oxysporum pathogen in tomato plants. The result shows that clove extract, containing approximately 20% antifungal compounds primarily eugenol (11% of total peak area), inhibits Fusarium oxysporum growth. It also reduces the hydrolytic activity of cutinase crucial for fungal penetration, evidenced by decreased fluorescence halos in tests with rhodamine-B and olive oil media under UV light after adding 1%, 2%, and 3% clove extract. Further in vivo studies are needed to explore its direct effects as a plant inhibitor.
Resistance of two sugarcane genotypes towards Sporisorium scitamineum Scoring Using Novel Method on Sugarcane buds tissue and Phenol Plant dynamics Supriyono, Supriyono; Abadi, Abdul Latief; Syib’li, Muhammad Akhid; Hidayah, Nurul
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.1

Abstract

Smut disease caused by a basidiomycete fungus, Sporisorium scitamineum, is one of major diseases on sugarcane worldwide. The disease could cause considerable yield losses up to 60% on susceptible varieties. Understanding of resistance characteristics of sugarcane clones is essential to support development of resistant sugarcane varieties to smut fungus infection. This study aimed to determine characteristics of sugarcane genotypes J19R17-325/15/3-9 and ASA Agribun toward S. scitamineum infection and the role of phenolic compound on defense mechanism of sugarcane to smut infection. The research consisted of four treatments and was arranged according to the randomized block design with five replicates. The results revealed that J19R17-325/15/3-9 produced higher phenolic compound (0,192 mg/gr) rather than ASA Agribun (0,182 mg/gr). In addition, the distribution of fungal mycelia within sugarcane tissues on J19R17-325/15/3-9 was also lower than those on ASA Agribun. These results indicated that J19R17-325/15/3-9 might have resistance properties to S. scitamineum infection.
The Use of Beneficial Microbes Tolerant of the Pyraclostrobin Fungicide, in Controlling the Pathogen Colletotrichum, the Cause of Anthracnose Disease in Chili Plants Tubagus, Satria Wahyu; Syib’li, Muhammad Akhid; Abadi, Abdul Latief
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.1

Abstract

Global production of chilies is significantly influenced by various biotic and abiotic factors. anthracnose is likely the main inhibiting factor reducing chili productivity in various countries. The use chemicals still formed the basis of disease control. Trichoderma sp. and FB1 bacterial is one such tolerant beneficial microbe belonging to the fungus and bacteria group that can survive under chemical pressure and exhibit antagonistic effects against the pathogen Colletotrichum sp. by the in vitro assay, it was found that in the antagonistic of Colletotrichum sp. with bacteria FB1 and Trichoderma sp., there was a significant difference, that the growth of Colletotrichum sp. could be inhibited by 69%. In the compatibility assay of bacteria FB1 and Trichoderma sp., no significant antagonistic response was found from both beneficial microbes. In the fungicide tolerance test of all isolates, it was found that beneficial microbes could still grow on growth media with a mixture of pyraclostrobin fungicide at a dose of 0.25 ul/ml. FTIR analysis showed the functional groups of the secondary metabolites of both the antagonistic bacteria and the antagonistic fungi had very high similarity in O-H and C=O groups. However, further research is needed on the mechanism of the secondary metabolites of the beneficial microbes involved in the inhibition of the pathogen Colletotrichum sp. 
Co-Authors Abdul Latief Abadi Aminudin Afandhi Fernando, Ito Istifada, Valia Karina, Anisya Putri Khairunnisa', Amalia Limin, Atfritedy Luqman Qurata Aini NURUL HIDAYAH Pamungkas, Bayu Aji Ratdiana, Ratdiana Retno Dyah Puspitarini Rina Rachmawati Sektiono, Antok Wahyu Supriyono Supriyono Taruna, Ardiyan tubagus, satria wahyu Yogo Setiawan