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Exploration of Symbiotic Bacteria with the Potential to Degrade Chlorpyrifos Insecticide in the Digestive Tract of the Beet Armyworm (Spodoptera exigua) (Lepidoptera: Noctuidae) in Shallot Fields, Probolinggo SyamsulHadi, Mochammad; Restikasari, Viera Annisa; Taufiqurrahman, Achmad Fitriadi; Widjayanti, Tita
Plantropica: Journal of Agricultural Science Vol. 9 No. 2 (2024): Agustus
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpt.2024.009.2.01

Abstract

The beet armyworm, Spodoptera exigua is a notorious pest that inflicts significant damage to shallot crops, especially leeks, causing leaves to wither due to brown spots and transparency. This issue often leads to excessive chemical control measures, which, in turn, promote insecticide resistance. This study hypothesized that symbiotic bacteria within S.exigua have the capacity to degrade chlorpyrifos-based insecticides. S.exigua samples were collected in Dringu, Gending, and Leces districts, Probolinggo, and subsequently examined for symbiotic bacteria with biodegradation potential. Bacterial exploration and identification were conducted from April to October 2023 at the Pesticide Toxicology Laboratory, Brawijaya University. The study analyzed the clear zone diameter produced by bacteria in media contaminated with chlorpyrifos-based insecticides as an indicator of biodegradation. Out of 41 surviving bacterial isolates, 10 demonstrated notable biodegradation capabilities. The Genus identification revealed that Erwinia sp., Pantoea sp., and Coryneform sp. were among the contributing bacteria. This research emphasizes the potential role of S.exigua-associated symbiotic bacteria in managing chlorpyrifos-resistant pest populations, offering a promising avenue for sustainable pest control methods.
The Effectiveness Test of Trichoderma harzianum, Mycorrhiza and Bacteria B8 on the Growth of Phytophthora nicotianae in Pineapple Plants (Ananas comosus L. Merr) at PT.Great Giant Pineapple Larasati, Candra Violita; Syamsulhadi, Mochammad; Widjayanti, Tita
Journal of Tropical Plant Protection Vol. 4 No. 2 (2023)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2023.004.2.5

Abstract

This study aims to determine the potential of biological agents to inhibit the pathogen P.  nicotianae that causes liver rot and the effect of biological agents on the growth of pineapple plants at PT. Great Giant Pineapple. The research was carried out at the Experimental Field and Research and Development Protection Laboratory of PT. Great Giant Pineapple, Terbanggi Besar, Central Lampung from September to November 2022. The research was carried out in several stages, namely the preparation stage and the implementation stage. The preparation stage includes land preparation, breeding and application of T. harzianum, preparation of liquid culture of T. harzianum and B8 bacteria, dipping crowns, and selection of crowns and tagging. While the implementation phase includes planting, application of T. harzianum, mycorrhiza, and B8 bacteria, observation of disease occurrence level, antagonist test, observation of new leaf growth, and plant observation. The data obtained were analyzed using the T-test, which compared the control plot with the treatment plot (T. harzianum, mycorrhiza, and B8 bacteria). The results showed that the application of the biological agent T. harzianum had a significant effect on suppressing the pathogen P. nicotianae that causes heart rot in pineapple plants in vitro. In addition, the application of T. harzianum, mycorrhiza, and B8 bacteria significantly affected the growth of the number of tillers, d-Leaf height, and d-Leaf index.
Induced Resistance of Rice Plants to Brown Planthoppers (Nilaparvata lugens Stål.) Through The Application of Compost SyamsulHadi, Mochammad; Taufiqurrahman, Achmad Fitriadi; Rahardjo, Bambang Tri; Tarno, Hagus
Plantropica: Journal of Agricultural Science Vol. 8 No. 2 (2023)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpt.2023.008.2.09

Abstract

The brown planthopper (Nilaparvata lugens Stål) poses a major threat to rice plants. Integrated Pest Management (IPM) emphasizes induced resistance as a cost-effective and sustainable strategy for protection. This research aims to investigate the impact of compost on rice plant resistance against brown planthoppers, the levels of silicate (SiO2) content and stem hardness in rice, and the mechanisms of resistance in rice plants attributed to compost. The research was conducted from June to November 2022 at the screen house of the Faculty of Agriculture, Brawijaya University and the Pest Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Brawijaya University. Analysis of silicate content (SiO2) was carried out at the Indonesian Sugar Plantation Research Center Laboratory. Analysis of the hardness lever of rice stem was carried out at the Food Quality and Safety Testing Laboratory, Faculty of Agricultural Technology, Brawijaya University. A randomized design featured 5 treatments and 5 replications, including varying compost doses (8, 11, 14, and 17 t ha-1) and a compost-free control. Parameters observed were brown planthopper mortality, damage intensity, silicate content, and stem hardness. Results exhibited compost is positive impact on brown planthopper mortality, reduced damage, higher silicate content, and increased stem hardness with higher compost doses. This research highlights compost is potential in enhancing rice plant resistance and protecting crops in an environmentally friendly manner.
IDENTIFIKASI SERANGGA HAMA PASCAPANEN KOMODITAS EKSPOR BIJI KOPI (Coffea sp.) TUJUAN JEPANG DAN BIJI PINANG (Areca catechu L.) TUJUAN IRAN MELALUI BALAI BESAR KARANTINA HEWAN, IKAN DAN TUMBUHAN (BBKHIT) SUMATERA UTARA: IDENTIFICATION OF POSTHARVEST INSECT PESTS ON EXPORT COMMODITIES OF COFFEE BEANS (Coffea sp.) TO JAPAN AND ARECA NUTS (Areca catechu L.) TO IRAN THROUGH THE NORTH SUMATERA CENTER FOR ANIMAL, FISH, AND PLANT QUARANTINE (BBKHIT) Pasaribu, Debora; Syamsulhadi, Mochammad
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2026.014.1.1

Abstract

Serangga hama pascapanen adalah serangga perusak yang menyerang produk di tempat penyimpanan dan dapat bertahan di dalam gudang. Kondisi lembab dan hangat serta ketersediaan makanan yang melimpah menciptakan lingkungan yang ideal bagi pertumbuhan hama ini. Tanaman kopi dari genus Coffea memiliki ciri morfologi unik, seperti batang tegak dan bercabang. Penelitian dilaksanakan dari Agustus hingga Oktober 2024 di gudang kopi PT Olam Indonesia, PT Mas Kawi Wijoyo, dan PT Royal Pacific tujuan Jepang, serta gudang biji pinang di PT Gajamukha, PT Usaha Rajin, dan PT VJ & J International tujuan Iran. Alat yang digunakan termasuk mikroskop stereo dan perangkap yellow sticky trap. Hasil penelitian menemukan tujuh spesies serangga hama di gudang kopi: A. fasciculatus, A. advena, C. ferrugineus, H. hampei, L. serricorne, L. entomophila, T. castaneum dan di gudang biji pinang ditemukan delapan spesies: A. fasciculatus, A. advena, C. ferrugineus, H. hampei, L. serricorne, L.  entomophila, O. surinamensis, T. castaneum. Ini menunjukkan bahwa perangkap yellow sticky trap efektif dalam menangkap serangga hama pada komoditas kopi dan pinang.